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Experiment: Comparative gene expression analysis between Arabidopsis wt and wrky33 mutant plants upon Botrytis cinerea infection
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-577
Wrky33 mutant plants are highly susceptible to the fungus Botrytis cinerea (Bc) while wt is not. To identify differentially expressed genes 5 weeks old wt and wrky33 (GABI_324B11) plants were mock treated and spray infected with Bc (isolate 2100 Spanish Type Culture Collection) at 5x105 spores/ml, and kept for 14 hours at high humidity and constant light. To resuspend the spores and for mock treatment Vogel buffer was used (in 1l: 15g sucrose, 3g Na-citrate, 5g K2HPO4, 0.2g MgSO4 7H2O, 0.1g CaCl2 2H2O, 2 g NH4NO3). 100 mg of leaf tissue was collected and total RNA isolated using TRI reagent following the suppliers instructions (Ambion). Leaves from three plants were pooled to make one biological replicate, and samples from three independent biological replicates for each genotype and treatment were submitted to hybridisation against a total number of 12 Arabidopsis ATH1 GeneChip arrays (Affymetrix).
About the ExperimenterName: | Dr Rainer Birkenbihl |
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Head of Lab Name: | Dr Imre Somssich |
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Lab:
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Address: | Max-Planck-Institute for Plant Breeding Dept. Plant-Microbe Interactions Carl-von-Linné Weg 10 Cologne
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Postcode:
| 50829 |
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Country:
| Germany |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| genetic_modification_design, pathogenicity_design |
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Number of Slides: | 12 |
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| Experimental Parameters:
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parameter | infect |
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Quality Control Measures Taken:
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Birkenbihl: Comparative gene expression analysis between Arabidopsis wt and wrky33 mutant plants upon Botrytis cinerea infection_genome
Slide: Birkenbihl_wt_contr_repl_1 | | |
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Tissue:
| rosette leaves |
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in vivo Treatment:
| mock treatment with Vogelbuffer |
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Additional Organism Information:
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Sample Description | The plants were 5 weeks old, had many rosette leaves and no buds. Typical short day plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Birkenbihl_wt_contr_repl_2 | | |
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Tissue:
| rosette leaves |
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in vivo Treatment:
| Mock-treatment with Vogelbuffer. |
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Additional Organism Information:
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Sample Description | The plants were 5 weeks old, had many rosette leaves and no buds. Typical short day plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Birkenbihl_wt_contr_repl_3 | | |
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Tissue:
| rosette leaves |
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in vivo Treatment:
| Mock-treatment with Vogelbuffer. |
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Additional Organism Information:
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Sample Description | The plants were 5 weeks old, had many rosette leaves and no buds. Typical short day plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Birkenbihl_wt_Bc_repl_1 | | |
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Tissue:
| rosette leaves |
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in vivo Treatment:
| The Botrytis cinerea isolate 2100 (also designated B. cinerea 1.29) from the Spanish Type Culture Collection was cultivated on potato dextrose plates at 22°C for 10 days. Spores were isolated, washed and frozen at -80 °C in 0.8 % NaCl at a concentration of 107 spores/ml. The Arabidopsis plants were spray infected with spores diluted in Vogelbuffer (in 1l: 15g sucrose, 3g Na-citrate, 5g K2HPO4, 0.2g MgSO4 7H2O, 0.1g CaCl2 2H2O, 2 g NH4NO3) to 5x105 spores/ml. For mock-treatment Vogelbuffer alone was used. One day before and during the entire infection process plants were incubated at high humidity and constant light under a hood. |
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Additional Organism Information:
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Sample Description | The plants were 5 weeks old, had many rosette leaves and no buds. Typical short day plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Birkenbihl_wt_Bc_repl_2 | | |
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Tissue:
| rosette leaves |
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in vivo Treatment:
| The Botrytis cinerea isolate 2100 (also designated B. cinerea 1.29) from the Spanish Type Culture Collection was cultivated on potato dextrose plates at 22°C for 10 days. Spores were isolated, washed and frozen at -80 °C in 0.8 % NaCl at a concentration of 107 spores/ml. The Arabidopsis plants were spray infected with spores diluted in Vogelbuffer (in 1l: 15g sucrose, 3g Na-citrate, 5g K2HPO4, 0.2g MgSO4 7H2O, 0.1g CaCl2 2H2O, 2 g NH4NO3) to 5x105 spores/ml. For mock-treatment Vogelbuffer alone was used. One day before and during the entire infection process plants were incubated at high humidity and constant light under a hood. |
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Additional Organism Information:
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Sample Description | The plants were 5 weeks old, had many rosette leaves and no buds. Typical short day plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Birkenbihl_wt_Bc_repl_3 | | |
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Tissue:
| rosette leaves |
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in vivo Treatment:
| The Botrytis cinerea isolate 2100 (also designated B. cinerea 1.29) from the Spanish Type Culture Collection was cultivated on potato dextrose plates at 22°C for 10 days. Spores were isolated, washed and frozen at -80 °C in 0.8 % NaCl at a concentration of 107 spores/ml. The Arabidopsis plants were spray infected with spores diluted in Vogelbuffer (in 1l: 15g sucrose, 3g Na-citrate, 5g K2HPO4, 0.2g MgSO4 7H2O, 0.1g CaCl2 2H2O, 2 g NH4NO3) to 5x105 spores/ml. For mock-treatment Vogelbuffer alone was used. One day before and during the entire infection process plants were incubated at high humidity and constant light under a hood. |
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Additional Organism Information:
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Sample Description | The plants were 5 weeks old, had many rosette leaves and no buds. Typical short day plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Birkenbihl_wrky33_contr_repl_1 | | |
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Tissue:
| rosette leaves |
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in vivo Treatment:
| mock treatment with Vogelbuffer |
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Additional Organism Information:
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Sample Description | The plants were 5 weeks old, had many rosette leaves and no buds. Typical short day plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Birkenbihl_wrky33_contr_repl_2 | | |
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Tissue:
| rosette leaves |
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in vivo Treatment:
| mock treatment with Vogelbuffer |
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Additional Organism Information:
| |
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Sample Description | The plants were 5 weeks old, had many rosette leaves and no buds. Typical short day plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Birkenbihl_wrky33_contr_repl_3 | | |
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Tissue:
| rosette leaves |
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in vivo Treatment:
| mock treatment with Vogelbuffer |
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Additional Organism Information:
| |
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Sample Description | The plants were 5 weeks old, had many rosette leaves and no buds. Typical short day plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Birkenbihl_wrky33_Bc_repl_1 | | |
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Tissue:
| rosette leaves |
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in vivo Treatment:
| The Botrytis cinerea isolate 2100 (also designated B. cinerea 1.29) from the Spanish Type Culture Collection was cultivated on potato dextrose plates at 22°C for 10 days. Spores were isolated, washed and frozen at -80 °C in 0.8 % NaCl at a concentration of 107 spores/ml. The Arabidopsis plants were spray infected with spores diluted in Vogelbuffer (in 1l: 15g sucrose, 3g Na-citrate, 5g K2HPO4, 0.2g MgSO4 7H2O, 0.1g CaCl2 2H2O, 2 g NH4NO3) to 5x105 spores/ml. For mock-treatment Vogelbuffer alone was used. One day before and during the entire infection process plants were incubated at high humidity and constant light under a hood. |
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Additional Organism Information:
| |
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Sample Description | The plants were 5 weeks old, had many rosette leaves and no buds. Typical short day plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Birkenbihl_wrky33_Bc_repl_2 | | |
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Tissue:
| rosette leaves |
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in vivo Treatment:
| The Botrytis cinerea isolate 2100 (also designated B. cinerea 1.29) from the Spanish Type Culture Collection was cultivated on potato dextrose plates at 22°C for 10 days. Spores were isolated, washed and frozen at -80 °C in 0.8 % NaCl at a concentration of 107 spores/ml. The Arabidopsis plants were spray infected with spores diluted in Vogelbuffer (in 1l: 15g sucrose, 3g Na-citrate, 5g K2HPO4, 0.2g MgSO4 7H2O, 0.1g CaCl2 2H2O, 2 g NH4NO3) to 5x105 spores/ml. For mock-treatment Vogelbuffer alone was used. One day before and during the entire infection process plants were incubated at high humidity and constant light under a hood. |
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Additional Organism Information:
| |
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Sample Description | The plants were 5 weeks old, had many rosette leaves and no buds. Typical short day plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Birkenbihl_wrky33_Bc_repl_3 | | |
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Tissue:
| rosette leaves |
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in vivo Treatment:
| The Botrytis cinerea isolate 2100 (also designated B. cinerea 1.29) from the Spanish Type Culture Collection was cultivated on potato dextrose plates at 22°C for 10 days. Spores were isolated, washed and frozen at -80 °C in 0.8 % NaCl at a concentration of 107 spores/ml. The Arabidopsis plants were spray infected with spores diluted in Vogelbuffer (in 1l: 15g sucrose, 3g Na-citrate, 5g K2HPO4, 0.2g MgSO4 7H2O, 0.1g CaCl2 2H2O, 2 g NH4NO3) to 5x105 spores/ml. For mock-treatment Vogelbuffer alone was used. One day before and during the entire infection process plants were incubated at high humidity and constant light under a hood. |
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Additional Organism Information:
| |
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Sample Description | The plants were 5 weeks old, had many rosette leaves and no buds. Typical short day plants. |
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Other Information:
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|
Protocols for BioSource 1 |
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Problems? Comments? Suggestions? Contact the Affymetrix Team