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Experiment: Effect of the explosive compound TNT on wild type sedlings
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-472
The aim of this experiment was to identify the genes involved in the detoxification of the toxic pollutant and explosive compound 2,4,6-trinitrotolune (TNT).Fourteen-day-old, liquid culture grown, Arabidopsis seedlings, ecotype Col0 (NASC stock code N1093), were dosed with 60 uM TNT dissolved in 60 ul dimethyl formamide (DMF) solvent, or 60 ul DMF only. After six hours, RNA was extracted and used for the microarray analysis.Further details and characterisation of glucosyltransferases identified using this method are presented in: Gandia-Herrero F, Lorenz A, Larson T, Graham I, Bowles D, Rylott E, Bruce N. Detoxification of the explosive 2,4,6-trinitrotoluene in Arabidopsis- discovery of bifunctional O- and C-glucosyltransferases (2008). The Plant Journal Dec;56(6):963-74.
About the ExperimenterName: | Dr Elizabeth Rylott |
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Head of Lab Name: | Prof Neil Bruce |
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Lab:
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Address: | CNAP Department of Biology University of York PO Box 373 York
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Postcode:
| YO10 5YW |
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Country:
| UK |
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| Telephone Number:
| 01904 328777 |
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Fax Number:
| 01904 328801 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| compound_treatment_design |
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Number of Slides: | 6 |
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| Experimental Parameters:
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parameter | compound_based_treatment |
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Quality Control Measures Taken:
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References:
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| Other Information:
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GEO accession | GSE18983 |
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Slides in this Experiment
Hybridisation Set: Rylott: Effect of the explosive compound TNT on wild type sedlings_genome
Slide: Rylott_1-4_DMF-treated-control-seedlings_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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Treatment | Seedlings in flasks were dosed with 60 microlitres dimethyl formamide (DMF)solvent |
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Other Information:
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sampledescription | Whole, 14-day-old seedlings |
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Protocols for BioSource 1 |
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Slide: Rylott_1-3_TNT-treated-seedlings_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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Treatment | Seedlings in flasks were dosed with 60 micromolar TNT dissolved in 60 microlitres dimethyl formamide (DMF)solvent |
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Other Information:
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sampledescription | Whole, 14-day-old seedlings |
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Protocols for BioSource 1 |
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Slide: Rylott_1-2_TNT-treated-seedlings_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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Treatment | Seedlings in flasks were dosed with 60 micromolar TNT dissolved in 60 microlitres dimethyl formamide (DMF)solvent |
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Other Information:
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sampledescription | Whole, 14-day-old seedlings |
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Protocols for BioSource 1 |
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Slide: Rylott_1-1_TNT-treated-seedlings_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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Treatment | Seedlings in flasks were dosed with 60 micromolar TNT dissolved in 60 microlitres dimethyl formamide (DMF)solvent |
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Other Information:
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sampledescription | Whole, 14-day-old seedlings |
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Protocols for BioSource 1 |
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Slide: Rylott_1-5_DMF-treated-control-seedlings_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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Treatment | Seedlings in flasks were dosed with 60 microlitres dimethyl formamide (DMF)solvent |
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Other Information:
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sampledescription | Whole, 14-day-old seedlings |
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Protocols for BioSource 1 |
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Slide: Rylott_1-6_DMF-treated-control-seedlings_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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Treatment | Seedlings in flasks were dosed with 60 microlitres dimethyl formamide (DMF)solvent |
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Other Information:
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sampledescription | Whole, 14-day-old seedlings |
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Protocols for BioSource 1 |
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