 | NASCArrays | ,
,
,
,
,
,
,
,
,
,
|
Experiment: Growth of suspension-cultured cells
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-381
The growth of Arabidopsis cell cultures following their sub-culture into fresh media follows standard growth kinetics of a period of exponential increase associated with high rate of cell division, followed by a slowing of the rate of increase as cells approach stationary phase. For the analysis described here, MM2d cells were subcultured into fresh MSS-medium and samples were taken at day 1, day3, day 5 and day7. We have carried out transcriptional profiling analysis with the aim to follow growth stage specific gene expression during unperturbed growth using the near full genome ATH1 arrays (Menges et al., 2003).
Journal Absract:
(Plant Molecular Biology: 53, 2003)
Plant cell suspension cultures are invaluable models for the study of cellular processes. Here we develop the recently described Arabidopsis suspension culture MM2d as a transcript profiling platform by means of Affymetrix ATH1 microarrays. Analysis of gene expression profiles during normal culture growth, during synchronous cell cycle re-entry and during synchronous cell cycle progression provides a unique integrated view of gene expression responses in a higher-plant system. Particularly striking is that expression of over 14 000 genes belonging to all defined categories can be reliably detected, suggesting that integrated and comparative analysis of data sets derived from transcript profiling of cultures is a powerful approach to identify candidate components involved in a wide range of biological processes. Combinatorial analysis of independent cell cycle synchrony methods allows the identification of genes that are apparently cell-cycle-regulated but are most likely responding to the induction of synchrony. We thus present an integrated genome-wide view of the transcriptional profile of a plant suspension culture and identify a refined set of 1082 cell cycle regulated genes largely independent of synchrony method.
About the ExperimenterName: | Dr Jim Murray |
---|
Head of Lab Name: | Dr Jim Murray |
---|
Lab:
| J Murray Laboratory |
---|
Institute:
| University of Cambridge |
---|
Address: | University of Cambridge Institute of Biotechnology Tennis Court Road Cambridge
|
---|
Postcode:
| CB2 1QT |
---|
Country:
| United Kingdom |
---|
| Telephone Number:
| 44 1223 334166 |
---|
Fax Number:
| 44 1223 334162 |
---|
All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
|
| About this ExperimentExperiment Type:
| time_series_design |
---|
Number of Slides: | 4 |
---|
| Experimental Parameters:
| |
---|
parameter | timepoint |
---|
Quality Control Measures Taken:
| |
---|
References:
| |
---|
reference | Menges et al., Plant Molecular Biology: 53, 2003 |
---|
| Other Information:
| |
---|
|
Slides in this Experiment
Hybridisation Set: Murray: Growth of suspension-cultured cells_genome
Slide: Murray_3-1_D1-GROWTH_Rep1_ATH1 | | |
|
|
|
Tissue:
| cell suspension |
---|
in vivo Treatment:
| |
---|
treatment | An aliquot of 5 ml of an early stationary phase MM2d cell suspension (7 days after previous subculture) was inoculated into 100 ml fresh MSS-medium (MS-salt, 3% sucrose, 0.5mg/l NAA, 0.05mg/l kinetin). Cells were incubated under continuous darkness by rotating at 130 rpm at a temperature of 27C and samples were taken every two days for analysis. The D1 sample was taken at day 1 after subculture into fresh medium (exp. Variable; time course). |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Murray_3-2_D3-GROWTH_Rep1_ATH1 | | |
|
|
|
Tissue:
| cell suspension |
---|
in vivo Treatment:
| |
---|
treatment | An aliquot of 5 ml of an early stationary phase MM2d cell suspension (7 days after previous subculture) was inoculated into 100 ml fresh MSS-medium (MS-salt, 3% sucrose, 0.5mg/l NAA, 0.05mg/l kinetin). Cells were incubated under continuous darkness by rotating at 130 rpm at a temperature of 27C and samples were taken every two days for analysis. The D3 sample was taken at day 3 after subculture into fresh medium (exp. variable; time course). |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Murray_3-3_D5-GROWTH_Rep1_ATH1 | | |
|
|
|
Tissue:
| cell suspension |
---|
in vivo Treatment:
| |
---|
treatment | An aliquot of 5 ml of an early stationary phase MM2d cell suspension (7 days after previous subculture) was inoculated into 100 ml fresh MSS-medium (MS-salt, 3% sucrose, 0.5mg/l NAA, 0.05mg/l kinetin). Cells were incubated under continuous darkness by rotating at 130 rpm at a temperature of 27C and samples were taken every two days for analysis. The D5 sample was taken at day 5 after subculture into fresh medium (exp. variable; time course). |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Murray_3-4_D7-GROWTH_Rep1_ATH1 | | |
|
|
|
Tissue:
| cell suspension |
---|
in vivo Treatment:
| |
---|
treatment | An aliquot of 5 ml of an early stationary phase MM2d cell suspension (7 days after previous subculture) was inoculated into 100 ml fresh MSS-medium (MS-salt, 3% sucrose, 0.5mg/l NAA, 0.05mg/l kinetin). Cells were incubated under continuous darkness by rotating at 130 rpm at a temperature of 27C and samples were taken every two days for analysis. The D7 sample was taken at day 7 after subculture into fresh medium (exp. variable; time course). |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Problems? Comments? Suggestions? Contact the Affymetrix Team