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Experiment: GA Treatment of Arabidopsis Root Tips
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-385
The plant hormone gibberellin represents an important regulator of growth and development. Early transcriptional events controlled by GA are not well characterised. Previous microarray studies have identified genes responsive to GA treatment in the whole seedling. The whole seedling represents many tissues where subtle effects of GA treatment in specific tissues may be masked. When treated with GA an effect on the growth rate of roots was observed. More specifically the shorter root of a GA deficient plant can be rescued to wild type length by the application of GA.
This experiment was designed to identify GA regulated genes in the root tips of Arabidopsis. The use of a GA deficient mutant provides a greater potential to identify genes responding to GA treatment. Root tips are ideally suited for the quick uptake of the hormone treatment. There will be two biological replicates which will each consist of a control treatment at 0 minutes and 2 hours as well as the experimental GA treated 2 hour time point. This system provides an opportunity to compare gene expression between treated and non-treated root tips and allow the identification of early GA responsive genes.
About the ExperimenterName: | Miss Jayne Griffiths |
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Head of Lab Name: | Professor Peter Hedden |
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Lab:
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Institute:
| Rothamsted Research |
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Address: | CPI Rothamsted Research Harpenden Hertfordshire
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Postcode:
| AL5 2JQ |
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Country:
| UK |
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| Telephone Number:
| 01582763133 |
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Fax Number:
| 01582760981 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| genetic_modification_design; compound_treatment_design; time_series_design |
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Number of Slides: | 6 |
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| Experimental Parameters:
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parameter | gene_knock_out |
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parameter | compound_based_treatment |
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parameter | timepoint |
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Quality Control Measures Taken:
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Griffiths: GA Treatment of Arabidopsis Root Tips_genome
Slide: Griffiths_1-1_AtCON0.1_Rep1_ATH1 | | |
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Tissue:
| root tips |
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in vivo Treatment:
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Treatment | Roots were grown on the surface of plates, they were acclimatised to being submerged for 24 hours prior to GA treatment. After 24 hours plates were transferred from the acclimatisation solution to an identical solution +/- 2µM GA. Treated samples were harvested after 0 hours. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Griffiths_1-2_AtCON120.1_Rep1_ATH1 | | |
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Tissue:
| root tips |
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in vivo Treatment:
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Treatment | Roots were grown on the surface of plates, they were acclimatised to being submerged for 24 hours prior to GA treatment. After 24 hours plates were transferred from the acclimatisation solution to an identical solution +/- 2µM GA. Treated samples were harvested after 0 hours. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Griffiths_1-3_AtGA120.1_Rep1_ATH1 | | |
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Tissue:
| root tips |
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in vivo Treatment:
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Treatment | Roots were grown on the surface of plates, they were acclimatised to being submerged for 24 hours prior to GA treatment. After 24 hours plates were transferred from the acclimatisation solution to an identical solution +/- 2µM GA. Treated samples were harvested after 0 hours. |
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Additional Organism Information:
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Allele knocked out | Phaseolus coccineus GA2ox1 |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Griffiths_1-4_AtCON0.2_Rep2_ATH1 | | |
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Tissue:
| root tips |
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in vivo Treatment:
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Treatment | Roots were grown on the surface of plates, they were acclimatised to being submerged for 24 hours prior to GA treatment. After 24 hours plates were transferred from the acclimatisation solution to an identical solution +/- 2µM GA. Treated samples were harvested after 0 hours. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Griffiths_1-5_AtCON120.2_Rep2_ATH1 | | |
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Tissue:
| root tips |
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in vivo Treatment:
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Treatment | Roots were grown on the surface of plates, they were acclimatised to being submerged for 24 hours prior to GA treatment. After 24 hours plates were transferred from the acclimatisation solution to an identical solution +/- 2µM GA. Treated samples were harvested after 0 hours. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Griffiths_1-6_AtGA120.2_Rep2_ATH1 | | |
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Tissue:
| root tips |
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in vivo Treatment:
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Treatment | Roots were grown on the surface of plates, they were acclimatised to being submerged for 24 hours prior to GA treatment. After 24 hours plates were transferred from the acclimatisation solution to an identical solution +/- 2µM GA. Treated samples were harvested after 0 hours. |
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Additional Organism Information:
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Allele knocked out | Phaseolus coccineus GA2ox1 |
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Other Information:
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Protocols for BioSource 1 |
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