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Experiment: Expression patterns of genes induced by sugar accumulation during early leaf senescence
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-136
The aim of our experiment is to analyse the expression pattern of genes induced by sugar accumulation during early leaf senescence.We have recently shown that senescence is induced by glucose in combination with low, but not with high nitrogen supply (Wingler et al. (2004), New Phytol, in press).
We would like to use Affymetrix analysis to compare senescence-specific gene expression in plants grown on
(i) low nitrogen without glucose (LNM),
(ii) low nitrogen with 2% glucose (LNG) and
(iii) high nitrogen with 2% glucose (HNG).
Comparison of (i) and (ii) will allow us to determine the effect of glucose, while comparison of (ii) and (iii) will reveal effects of nitrogen supply.
For this experiment, plants will be harvested on day 30, when first signs of senescence become visible on the LNG medium. All samples will be taken around mid-day. For each sample, 12 plants from 3 different petri dishes will be pooled. We are planning to analyse 3 independent samples per treatment, i.e. 9 samples in total. RNA will be extracted from whole leaf rosettes (without roots and inflorescences) using the RNeasy Plant Kit (Qiagen).
At a later stage, we are also intending to compare gene expression in wild-type plants with expression in the abi5-1 mutant (Ws-2 background), which shows a delay in sugar-induced senescence (Wingler, Astrid, Marès, Magali & Pourtau, Nathalie (2004)
Spatial patterns and metabolic regulation of photosynthetic parameters during leaf senescence.
New Phytologist 161 (3), 781-789). We have therefore chosen to use Ws-2 for the experiment proposed here. The experiment with abi5-1 will be conducted once we have obtained data on treatment-specific effects in Ws-2.
About the ExperimenterName: | Dr Nathalie Pourtau |
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Head of Lab Name: | Dr Astrid Wingler |
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Lab:
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Institute:
| University College London |
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Address: | University College London, Biology Department, Gower Street, LONDON.
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Postcode:
| WC 1E 6BT |
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Country:
| UK |
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| Telephone Number:
| 020 7679 7268 |
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Fax Number:
| 020 7679 7096 |
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are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
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co-authors in the work.
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| About this ExperimentExperiment Type:
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Number of Slides: | 9 |
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| Experimental Parameters:
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parameter | nutrients |
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Quality Control Measures Taken:
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no-plants-pooled | 12 |
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Pourtau_genome
Slide: Pourtau_1-9_highN-glu_Rep3_ATH1 | | |
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Tissue:
| whole leaf rosettes without the roots and inflorescences |
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Diseased:
| Normal |
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in vivo Treatment:
| Half-strength Murashige and Skoog medium containing 30 mM nitrogen (10.3mM NH4+ and 19.7mM NO3-). For sugar treatments, 2% of glucose will be added to the corresponding medium. |
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Additional Organism Information:
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tissue | We are going to use whole leaf rosettes without the roots and inflorescences (to avoid treatment-dependent effects on flowers-specific gene expression). |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Pourtau_1-6_lowN-glu_Rep3_ATH1 | | |
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Tissue:
| whole leaf rosettes without the roots and inflorescences |
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Diseased:
| Normal |
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in vivo Treatment:
| Half-strength Murashige and Skoog medium containing 4.7mM (NO3- only). For sugar treatments, 2% of glucose will be added to the corresponding medium. |
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Additional Organism Information:
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tissue | We are going to use whole leaf rosettes without the roots and inflorescences (to avoid treatment-dependent effects on flowers-specific gene expression). |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Pourtau_1-3_lowN_Rep3_ATH1 | | |
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Tissue:
| whole leaf rosettes without the roots and inflorescences |
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Diseased:
| Normal |
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in vivo Treatment:
| Half-strength Murashige and Skoog medium containing 4.7mM (NO3- only). |
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Additional Organism Information:
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tissue | We are going to use whole leaf rosettes without the roots and inflorescences (to avoid treatment-dependent effects on flowers-specific gene expression). |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Pourtau_1-8_highN-glu_Rep2_ATH1 | | |
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Tissue:
| whole leaf rosettes without the roots and inflorescences |
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Diseased:
| Normal |
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in vivo Treatment:
| Half-strength Murashige and Skoog medium containing 30 mM nitrogen (10.3mM NH4+ and 19.7mM NO3-). For sugar treatments, 2% of glucose will be added to the corresponding medium. |
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Additional Organism Information:
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tissue | We are going to use whole leaf rosettes without the roots and inflorescences (to avoid treatment-dependent effects on flowers-specific gene expression). |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Pourtau_1-5_lowN-glu_Rep2_ATH1 | | |
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Tissue:
| whole leaf rosettes without the roots and inflorescences |
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Diseased:
| Normal |
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in vivo Treatment:
| Half-strength Murashige and Skoog medium containing 4.7mM (NO3- only). For sugar treatments, 2% of glucose will be added to the corresponding medium. |
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Additional Organism Information:
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tissue | We are going to use whole leaf rosettes without the roots and inflorescences (to avoid treatment-dependent effects on flowers-specific gene expression). |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Pourtau_1-2_lowN_Rep2_ATH1 | | |
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Tissue:
| whole leaf rosettes without the roots and inflorescences |
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Diseased:
| Normal |
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in vivo Treatment:
| Half-strength Murashige and Skoog medium containing 4.7mM (NO3- only). |
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Additional Organism Information:
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tissue | We are going to use whole leaf rosettes without the roots and inflorescences (to avoid treatment-dependent effects on flowers-specific gene expression). |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Pourtau_1-7_highN-glu_Rep1_ATH1 | | |
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Tissue:
| whole leaf rosettes without the roots and inflorescences |
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Diseased:
| Normal |
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in vivo Treatment:
| Half-strength Murashige and Skoog medium containing 30 mM nitrogen (10.3mM NH4+ and 19.7mM NO3-). For sugar treatments, 2% of glucose will be added to the corresponding medium. |
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Additional Organism Information:
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tissue | We are going to use whole leaf rosettes without the roots and inflorescences (to avoid treatment-dependent effects on flowers-specific gene expression). |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Pourtau_1-4_lowN-glu_Rep1_ATH1 | | |
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Tissue:
| whole leaf rosettes without the roots and inflorescences |
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Diseased:
| Normal |
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in vivo Treatment:
| Half-strength Murashige and Skoog medium containing 4.7mM (NO3- only). For sugar treatments, 2% of glucose will be added to the corresponding medium. |
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Additional Organism Information:
| |
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tissue | We are going to use whole leaf rosettes without the roots and inflorescences (to avoid treatment-dependent effects on flowers-specific gene expression). |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Pourtau_1-1_lowN_Rep1_ATH1 | | |
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Tissue:
| whole leaf rosettes without the roots and inflorescences |
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Diseased:
| Normal |
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in vivo Treatment:
| Half-strength Murashige and Skoog medium containing 4.7mM (NO3- only). |
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Additional Organism Information:
| |
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tissue | We are going to use whole leaf rosettes without the roots and inflorescences (to avoid treatment-dependent effects on flowers-specific gene expression). |
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Other Information:
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|
Protocols for BioSource 1 |
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Problems? Comments? Suggestions? Contact the Affymetrix Team