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Experiment: Xylella fastidiosa infection of Arabidopsis thaliana Tsu-1
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-582
The experiment compares Xylella fastidiosa (GFP labeled Temecula) inoculated Arabidopsis with mock inoculated tissue. Bacterial were mechanically inoculated into midrib of leaves. At 9dpi, midribs above the inoculation point were dissected out and frozen. Total RNA was isolated using Trizol, and hybridized to Affy ATH-1 arrays. Three biological replicate pairs of Xf and mock inoculated RNA were run.
About the ExperimenterName: | Elizabeth Rogers |
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Head of Lab Name: | Elizabeth Rogers |
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Lab:
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Address: | USDA-ARS-SJVASC 9611 S. Riverbend Ave Parlier, CA
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Postcode:
| 93648 |
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Country:
| United States |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| pathogenicity_design |
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Number of Slides: | 6 |
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| Experimental Parameters:
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parameter | infect |
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Quality Control Measures Taken:
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biological-replicates | 3 |
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References:
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Slides in this Experiment
Hybridisation Set: Rogers: Xylella fastidiosa infection of Arabidopsis thaliana Tsu-1_genome
Slide: Rogers_Xylella-fastidiosa inoculated 3_SLD | | |
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Tissue:
| leaf midrib from inoculated leaf |
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in vivo Treatment:
| plants were 4-4.5 weeks old at inoculation. Xylella fastidiosa (GFP Temecula from Steve Lindow) inoculation - 1 ul drop of bacteria OD600 = 0.2 in water on midrib at leaf-petiole junction and poked 4-5 times under drop with very small pin. harvest 9 dpi |
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Additional Organism Information:
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Sample Description | fully mature plant, prior to bolting |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Rogers_Xylella-fastidiosa inoculated 2_SLD | | |
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Tissue:
| leaf midrib from inoculated leaf |
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in vivo Treatment:
| plants were 4-4.5 weeks old at inoculation. Xylella fastidiosa (GFP Temecula from Steve Lindow) inoculation - 1 ul drop of bacteria OD600 = 0.2 in water on midrib at leaf-petiole junction and poked 4-5 times under drop with very small pin. harvest 9 dpi |
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Additional Organism Information:
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Sample Description | fully mature plant, prior to bolting |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Rogers_Xylella-fastidiosa inoculated 1_SLD | | |
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Tissue:
| leaf midrib from inoculated leaf |
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in vivo Treatment:
| plants were 4-4.5 weeks old at inoculation. Xylella fastidiosa (GFP Temecula from Steve Lindow) inoculation - 1 ul drop of bacteria OD600 = 0.2 in water on midrib at leaf-petiole junction and poked 4-5 times under drop with very small pin. harvest 9 dpi |
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Additional Organism Information:
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Sample Description | fully mature plant, prior to bolting |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Rogers_mock-inoculated 3_SLD | | |
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Tissue:
| leaf midrib |
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in vivo Treatment:
| plants were 4-4.5 weeks old at inoculation. mock inoculation - 1 ul drop of water on midrib at leaf-petiole junction and poked 4-5 times under drop with very small pin. harvest 9 dpi |
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Additional Organism Information:
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Sample Description | fully mature plant, prior to bolting |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Rogers_mock-inoculated 2_SLD | | |
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Tissue:
| leaf midrib |
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in vivo Treatment:
| plants were 4-4.5 weeks old at inoculation. mock inoculation - 1 ul drop of water on midrib at leaf-petiole junction and poked 4-5 times under drop with very small pin. harvest 9 dpi |
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Additional Organism Information:
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Sample Description | fully mature plant, prior to bolting |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Rogers_mock-inoculated 1_SLD | | |
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Tissue:
| leaf midrib |
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in vivo Treatment:
| plants were 4-4.5 weeks old at inoculation. mock inoculation - 1 ul drop of water on midrib at leaf-petiole junction and poked 4-5 times under drop with very small pin. harvest 9 dpi |
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Additional Organism Information:
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Sample Description | fully mature plant, prior to bolting |
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Other Information:
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Protocols for BioSource 1 |
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