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Experiment: NPA-induced vascular overgrowth in Arabidopsis
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-428
We used NPA-induced vascular overgrowth in Arabidopsis leaves to look for differential up-regulation of genes in NPA-treated tissues that may be involved in vascular differentiation. Arabidopsis thaliana Col-0 plants were grown for approximately 2 weeks on solid ATS medium (1) containing a final concentration of 10 um NPA (dissolved in DMSO) or an equivalent volume of DMSO (control). At this stage plants had approximately 6 rosette leaves. RNA was prepared from entire shoot tissues of control (DMSO) or NPA-treated plants.(1) Lincoln et al., 1990. Plant Cell 2: 1071-1080.
About the ExperimenterName: | Dr Carol Wenzel |
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Head of Lab Name: | Dr Jim Mattsson |
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Lab:
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Address: | Department of Biological Sciences Simon Fraser University 8888 University Drive Burnaby, BC
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Postcode:
| V5A 1S6 |
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Country:
| Canada |
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| Telephone Number:
| 17787824391 |
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Fax Number:
| 17787823496 |
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are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| compound_treatment_design; |
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Number of Slides: | 2 |
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| Experimental Parameters:
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parameter | compound_based_treatment |
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Quality Control Measures Taken:
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References:
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| Other Information:
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GEO Accession | GSE19262 |
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Slides in this Experiment
Hybridisation Set: Wenzel: NPA-induced vascular overgrowth in Arabidopsis_genome
Slide: Wenzel_1-1_DMSO-control-2wk-shoots_Rep1_ATH1 | | |
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Tissue:
| shoot |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Wenzel_1-2_NPA-treated-2wk-shoots_Rep1_ATH1 | | |
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Tissue:
| shoot |
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in vivo Treatment:
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treatment | added N-(1-Naphthyl) phthalamic acid (NPA; Sigma, St. Louis, MO) dissolved in DMSO to a final concentration of 10 uM |
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Other Information:
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Protocols for BioSource 1 |
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