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Experiment: The effects of mutants in stress response pathways on gene expression during senescence
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-52
Many signalling pathways are involved in controlling gene expression during plant senescence. Pathways involving SA, JA and ethylene have a role in senescence but none are essential for the senescence process to occur. The aim of this experiment is to classify senescence-enhanced genes into groups depending on the signalling pathways that regulate them. This will provide useful information on the relative importance of each signalling pathway during senescence and allow us to separate potential senescence-specific genes and pathways from the stress response pathways.Mutants in genes in the ethylene pathway (ein2) and the jasmonate pathway (coi1) and the NahG transgenic plant which is defective in the salicylic acid pathway will be grown until the mid flowering stage. Fully developed green and partially senescent leaves will be harvested from the plants at this stage. In addition, two different lines of Arabidopsis (Col5 glabrous and Col-0) will be grown as controls. Leaves will be harvested from the two control plants before flowering (green) and at mid flowering as above. The control plants will be harvested at two stages to identify the senescence- enhanced genes. The effects of each mutation on the senescence related expression of these genes will then be studied.Mutant RNAs will be isolated in duplicate. The two control accessions will act as replicates for the wild type. Two wild type accessions will be used to reduce possible differences that could be observed the mutants due to slight differences in background.
About the ExperimenterName: | Dr Vicky Buchanan-Wollaston |
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Head of Lab Name: | |
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Lab:
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Institute:
| Horticulture Research International |
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Address: | Horticulture Research International Wellesbourne Warwick
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Postcode:
| CV35 9EF |
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Country:
| UK |
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| Telephone Number:
| 01789 470382 |
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Fax Number:
| 01789 470552 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| genetic_modification_design; development_or_differentiation_design |
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Number of Slides: | 10 |
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| Experimental Parameters:
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parameter | developmental_stage |
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parameter | gene_knock_out |
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Quality Control Measures Taken:
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no-plants-pooled | leaves from 10-20 plants per pool |
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References:
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| Other Information:
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ArrayExpressAccession | E-NASC-17 |
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Slides in this Experiment
Hybridisation Set: Buchanan-Wollaston_genome
Slide: Buchanan-Wollaston_A-1-bwoll-C0G_SLD | | |
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Tissue:
| fully expanded leaves |
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Diseased:
| Normal |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Buchanan-Wollaston_A-2-bwoll-C5G_SLD | | |
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Tissue:
| fully expanded leaves |
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Diseased:
| Normal |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Buchanan-Wollaston_A-3-bwoll-C0S_SLD | | |
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Tissue:
| fully expanded leaves |
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Diseased:
| Normal |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Buchanan-Wollaston_A-4-bwoll-C5S_SLD | | |
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Tissue:
| fully expanded leaves |
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Diseased:
| Normal |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Buchanan-Wollaston_A-5-bwoll-NG1_SLD | | |
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Tissue:
| fully expanded leaves |
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Diseased:
| Normal |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Buchanan-Wollaston_A-6-bwoll-NG2_SLD | | |
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Tissue:
| fully expanded leaves |
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Diseased:
| Normal |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Buchanan-Wollaston_A-7-bwoll-Ei1_SLD | | |
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Tissue:
| fully expanded leaves |
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Diseased:
| Normal |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Buchanan-Wollaston_A-8-bwoll-Ei2_SLD | | |
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Tissue:
| fully expanded leaves |
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Diseased:
| Normal |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Buchanan-Wollaston_A-9-bwoll-Co1_SLD | | |
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Tissue:
| fully expanded leaves |
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Diseased:
| Normal |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Buchanan-Wollaston_A-10-bwoll-Co2_SLD | | |
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Tissue:
| fully expanded leaves |
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Diseased:
| Normal |
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Other Information:
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Protocols for BioSource 1 |
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