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Experiment: HAP5 Triple Mutant vs Constans Mutant
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-440
Aims:To compare the transcriptional profiles of a a AtHAP5 (AtNF-YC, AtCBF-C) triple mutant vs. Constans mutant. Background Information on Mutants:AtHAP5 Triple mutant created by cross between the following SALK TDNA inserts; SALK_034838, SALK_058903, SALK_032163 these correspond to At1G54830, At1G08970, At5G63470, respectively. co-SAIL TDNA insertion SAIL_24_H04 corresponds to AT5G15840 Tissue:Above ground whole plants grown for 10 days, under 16 hr light 8 hrs dark at constant 22ºC. Tissue collected 14 hrs after lights on. RT-PCR analysis on test RNA confirmed FT levels reduced in mutants lines compared to wild-type.
About the ExperimenterName: | Dr. Ben Holt |
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Head of Lab Name: | Dr. Ben Holt |
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Lab:
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Address: | University of Oklahoma Department of Botany and Microbiology 770 Van Vleet Oval Room 219 GLCH Norman, OK
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Postcode:
| 73019 |
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Country:
| USA |
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| Telephone Number:
| 4053259018 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| genetic_modification_design |
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Number of Slides: | 9 |
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| Experimental Parameters:
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parameter | gene_knock_out |
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Quality Control Measures Taken:
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References:
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Slides in this Experiment
Hybridisation Set: Holt: HAP5 Triple Mutant vs Constans Mutant_genome
Slide: Holt_1-1_HAP5-triple-mutant_Rep1_ATH1 | | |
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Tissue:
| Above ground |
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Additional Organism Information:
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Genes knocked out | At1G54830, At5G63470, At1G08970 |
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Sample description | Above ground tissue from 10 day old plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Holt_1-2_HAP5-triple-mutant_Rep2_ATH1 | | |
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Tissue:
| Above ground |
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Additional Organism Information:
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Genes knocked out | At1G54830, At5G63470, At1G08970 |
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Sample description | Above ground tissue from 10 day old plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Holt_1-3_HAP5-triple-mutant_Rep3_ATH1 | | |
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Tissue:
| Above ground |
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Additional Organism Information:
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Genes knocked out | At1G54830, At5G63470, At1G08970 |
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Sample description | Above ground tissue from 10 day old plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Holt_1-4_Constans-mutant_Rep1_ATH1 | | |
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Tissue:
| Above ground |
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Additional Organism Information:
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Sample description | Above ground tissue from 10 day old plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Holt_1-5_Constans-mutant_Rep2_ATH1 | | |
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Tissue:
| Above ground |
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Additional Organism Information:
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Sample description | Above ground tissue from 10 day old plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Holt_1-6_Constans-mutant_Rep3_ATH1 | | |
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Tissue:
| Above ground |
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Additional Organism Information:
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Sample description | Above ground tissue from 10 day old plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Holt_1-7_Columbia-control_Rep1_ATH1 | | |
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Tissue:
| Above ground |
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Additional Organism Information:
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Sample description | Above ground tissue from 10 day old plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Holt_1-8_Columbia-control_Rep2_ATH1 | | |
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Tissue:
| Above ground |
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Additional Organism Information:
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Sample description | Above ground tissue from 10 day old plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Holt_1-9_Columbia-control_Rep3_ATH1 | | |
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Tissue:
| Above ground |
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Additional Organism Information:
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Sample description | Above ground tissue from 10 day old plants |
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Other Information:
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Protocols for BioSource 1 |
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