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Experiment: Arabidopsis treated with nitrite and nitrate
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-481
Nitrate serves as a potent signal to control gene expression in plants and algae, but little is known about the signaling role of nitrite, the direct product of nitrate reduction. Analysis of several nitrate-induced genes showed that nitrite increases mRNA levels as rapidly as nitrate in nitrogen-starved Arabidopsis (Arabidopsis thaliana) roots. Both nitrite and nitrate induction are apparent at concentrations as low as 100 nm. The response at low nitrite concentrations was not due to contaminating nitrate, which was present at <1% of the nitrite concentration. High levels of ammonium (20 mm) in the growth medium suppressed induction of several genes by nitrate, but had varied effects on the nitrite response. Transcriptome analysis using 250 or 5 microm nitrate or nitrite showed that over one-half of the nitrate-induced genes, which included genes involved in nitrate and ammonium assimilation, energy production, and carbon and nitrogen metabolism responded equivalently to nitrite; however, the nitrite response was more robust and there were many genes that responded specifically to nitrite. Thus, nitrite can serve as a signal as well as if not better than nitrate.
About the ExperimenterName: | Dr. Rongchen Wang |
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Head of Lab Name: | Professor Nigel Crawford |
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Lab:
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Address: | University of California, San Diego Division of Biological Sciences 9500 Gilman Drive, 0116 La Jolla, CA 92093-0116 USA
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Postcode:
| 92093-0116 |
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Country:
| USA |
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| Telephone Number:
| 18585342519 |
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Fax Number:
| 18585347108 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| compound_treatment_design |
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Number of Slides: | 6 |
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| Experimental Parameters:
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parameter | compound_based_treatment |
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parameter | gene_knock_out |
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Quality Control Measures Taken:
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References:
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Reference | Plant Physiology 145:1735-1745 (2007) |
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Wang: Arabidopsis treated with nitrite and nitrate_genome
Slide: Wang_3-6_WT-Col-250-microM-KNO2-20min-roots_Rep2_ATH1 | | |
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Tissue:
| Roots |
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Diseased:
| Treatment |
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in vivo Treatment:
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Treatment | 250 µM KNO2 |
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Additional Organism Information:
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Sample description | WT Col., 250 microM KNO2, 20min, roots, replicate 2 |
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Other Information:
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Reference | Plant Physiology 145:1735-1745 (2007) |
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Protocols for BioSource 1 |
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Slide: Wang_3-4_WT-Col-250-microM-KCL-20min-roots_Rep2_ATH1 | | |
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Tissue:
| Roots |
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Diseased:
| Control |
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in vivo Treatment:
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Treatment | 250 µM KCl |
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Additional Organism Information:
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Sample description | WT Col., 250 microM KCL, 20min, roots, replicate 2 |
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Other Information:
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Reference | Plant Physiology 145:1735-1745 (2007) |
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Protocols for BioSource 1 |
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Slide: Wang_3-5_WT-Col-250-microM-KNO3-20min-roots_Rep2_ATH1 | | |
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Tissue:
| Roots |
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Diseased:
| Treatment |
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in vivo Treatment:
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Treatment | 250 µM KNO3 |
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Additional Organism Information:
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Sample description | WT Col., 250 microM KNO3, 20min, roots, replicate 2 |
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Other Information:
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Reference | Plant Physiology 145:1735-1745 (2007) |
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Protocols for BioSource 1 |
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Slide: Wang_3-2_WT-Col-250-microM-KNO3-20min-roots_Rep1_ATH1 | | |
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Tissue:
| Roots |
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Diseased:
| Treatment |
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in vivo Treatment:
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Treatment | 250 µM KNO3 |
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Additional Organism Information:
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Sample description | WT Col., 250 microM KNO3, 20min, roots, replicate 1 |
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Other Information:
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Reference | Plant Physiology 145:1735-1745 (2007) |
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Protocols for BioSource 1 |
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Slide: Wang_3-3_WT-Col-250-microM-KNO2-20min-roots_Rep1_ATH1 | | |
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Tissue:
| Roots |
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Diseased:
| Treatment |
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in vivo Treatment:
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Treatment | 250 µM KNO2 |
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Additional Organism Information:
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Sample description | WT Col., 250 microM KNO2, 20min, roots, replicate 1 |
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Other Information:
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Reference | Plant Physiology 145:1735-1745 (2007) |
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Protocols for BioSource 1 |
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Slide: Wang_3-1_WT-Col-250-microM-KCL-20min-roots_Rep1_ATH1 | | |
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Tissue:
| Roots |
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Diseased:
| Control |
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in vivo Treatment:
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Treatment | 250 µM KCl |
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Additional Organism Information:
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Sample description | WT Col., 250 microM KCL, 20min, roots, replicate 1 |
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Other Information:
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Reference | Plant Physiology 145:1735-1745 (2007) |
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Protocols for BioSource 1 |
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