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Experiment: Comparison of wt and biotin-deficient mutant
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-549
In this experiment we have compared gene expression in wild-type Arabidopsis COL-ecotype and a T-DNA insertion mutant bio4-1 that has an altered biotin synthesis. The bio4-1 plants were either grown normally or watered with 200 µM biotin solution.
About the ExperimenterName: | Elina Helenius |
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Head of Lab Name: | T. Palva |
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Lab:
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Institute:
| Adaptive Responses in Plants Group, Genetics, Department of Biological and Environmental Sciences, Faculty of Biosciences, University of Helsinki |
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Address: | P.O. Box 56 (Viikinkaari 5D), Helsinki, Helsingfors
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Postcode:
| 00014 |
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Country:
| FINLAND |
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| Telephone Number:
| +3589191959085 |
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Fax Number:
| +3589191959079 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| genetic_modification_design |
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Number of Slides: | 6 |
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| Experimental Parameters:
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parameter | gene_knock_out |
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Quality Control Measures Taken:
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Helenius: Comparison of wt and biotin-deficient mutant
Slide: Helenius_549-1_Col-0_Rep2_ATH1 | | |
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Tissue:
| rosette leaves |
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Additional Organism Information:
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Sample Description | Samples were collected from 35 day-old plants. Rosette leaves from five plants were pooled for one sample. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Helenius_549-2_Col-0_Rep3_ATH1 | | |
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Tissue:
| rosette leaves |
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Additional Organism Information:
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Sample Description | Samples were collected from 35 day-old plants. Rosette leaves from five plants were pooled for one sample. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Helenius_549-3_Bio4-1_Rep2_ATH1 | | |
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Tissue:
| rosette leaves |
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Additional Organism Information:
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Sample Description | Samples were collected from 35 day-old plants. Rosette leaves from five plants were pooled for one sample. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Helenius_549-4_Bio4-1_Rep3_ATH1 | | |
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Tissue:
| rosette leaves |
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Additional Organism Information:
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Sample Description | Samples were collected from 35 day-old plants. Rosette leaves from five plants were pooled for one sample. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Helenius_549-5_Bio4-1+Biotin__Rep2_ATH1 | | |
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Tissue:
| rosette leaves |
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in vivo Treatment:
| The biotin treatment was done by watering the plants with 200µM biotin (in tap water) twice a week. |
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Additional Organism Information:
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Sample Description | Samples were collected from 35 day-old plants. Rosette leaves from five plants were pooled for one sample. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Helenius_549-6_Bio4-1+Biotin_Rep3_ATH1 | | |
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Tissue:
| rosette leaves |
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in vivo Treatment:
| The biotin treatment was done by watering the plants with 200µM biotin (in tap water) twice a week. |
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Additional Organism Information:
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Sample Description | Samples were collected from 35 day-old plants. Rosette leaves from five plants were pooled for one sample. |
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Other Information:
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Protocols for BioSource 1 |
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