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Experiment: Auxin induced in-vitro cambium initiation in Atrabidopsis thaliana
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-503
An in-vitro system has been used to induce Cambium initiation in Arabidopsis thaliana stems via Auxin treatments. A time course experiment has been performed and LCM harvested samples from different tissue types have been hybridized. The aim of the project is to isolate and characterize important regulators for cambium initiation.
About the ExperimenterName: | Dr Javier Agusti |
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Head of Lab Name: | Dr Thomas Greb |
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Lab:
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Address: | Gregor Mendel Institute of Plant Molecular Biology, Austrian Academy of Sciences, Dr. Bohr-Gasse 3
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Postcode:
| 1030 |
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Country:
| Austria |
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| Telephone Number:
| +43-(0)1-79044-9871 |
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Fax Number:
| +43-(0)1-79044-23-9870 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| compound_treatment_design; time_series_design; strain_or_line_design |
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Number of Slides: | 24 |
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| Experimental Parameters:
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parameter | hormones |
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parameter | timepoint |
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parameter | cell_type |
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Quality Control Measures Taken:
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References:
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| Other Information:
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title | Auxin induced in-vitro cambium initiation in Atrabidopsis thaliana |
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Slides in this Experiment
Hybridisation Set: Agusti: Auxin induced in-vitro cambium initiation in Atrabidopsis thaliana_genome
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Tissue:
| Stem starch sheath layer + interfascicular fibers |
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in vivo Treatment:
| Stems from 15cm plants were harvested. Only plants which first internode was at least 5cm long were selected. Stems were surface sterylised and a 1.5cm sample located 2cm away from the rosette was separated for our experiments. The samples were rapidly embedded in OCT and frozen until used. |
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Additional Organism Information:
| |
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Sample Description | 15cm plants with a first internode at least 5cm long. |
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Other Information:
| |
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ecotype_habitat | |
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Timecourse start procedure | Start point was set when plant were collected. |
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|
Protocols for BioSource 1 |
|
|
|
|
|
|
Tissue:
| Stem starch sheath layer + interfascicular fibers |
---|
in vivo Treatment:
| Stems from 15cm plants were harvested. Only plants which first internode was at least 5cm long were selected. Stems were surface sterylised and a 1.5cm sample located 2cm away from the rosette was separated for our experiments. The samples were rapidly embedded in OCT and frozen until used. |
---|
Additional Organism Information:
| |
---|
Sample Description | 15cm plants with a first internode at least 5cm long. |
---|
Other Information:
| |
---|
ecotype_habitat | |
---|
Timecourse start procedure | Start point was set when plant were collected. |
---|
|
Protocols for BioSource 1 |
|
|
|
|
|
|
Tissue:
| Stem starch sheath layer + interfascicular fibers |
---|
in vivo Treatment:
| Stems from 15cm plants were harvested. Only plants which first internode was at least 5cm long were selected. Stems were surface sterylised and a 1.5cm sample located 2cm away from the rosette was separated for our experiments. The samples were rapidly embedded in OCT and frozen until used. |
---|
Additional Organism Information:
| |
---|
Sample Description | 15cm plants with a first internode at least 5cm long. |
---|
Other Information:
| |
---|
ecotype_habitat | |
---|
Timecourse start procedure | Start point was set when plant were collected. |
---|
|
Protocols for BioSource 1 |
|
|
|
|
|
|
Tissue:
| Stem except starch sheath layer and vascular bundles. |
---|
in vivo Treatment:
| Stems from 15cm plants were harvested. Only plants which first internode was at least 5cm long were selected. Stems were surface sterylised and a 1.5cm sample located 2cm away from the rosette was separated for our experiments. The samples were rapidly embedded in OCT and frozen until used. |
---|
Additional Organism Information:
| |
---|
Sample Description | 15cm plants with a first internode at least 5cm long. |
---|
Other Information:
| |
---|
ecotype_habitat | |
---|
Timecourse start procedure | Start point was set when plant were collected. |
---|
|
Protocols for BioSource 1 |
|
|
|
|
|
|
Tissue:
| Stem except starch sheath layer and vascular bundles. |
---|
in vivo Treatment:
| Stems from 15cm plants were harvested. Only plants which first internode was at least 5cm long were selected. Stems were surface sterylised and a 1.5cm sample located 2cm away from the rosette was separated for our experiments. The samples were rapidly embedded in OCT and frozen until used. |
---|
Additional Organism Information:
| |
---|
Sample Description | 15cm plants with a first internode at least 5cm long. |
---|
Other Information:
| |
---|
ecotype_habitat | |
---|
Timecourse start procedure | Start point was set when plant were collected. |
---|
|
Protocols for BioSource 1 |
|
|
|
|
|
|
Tissue:
| Stem except starch sheath layer and vascular bundles. |
---|
in vivo Treatment:
| Stems from 15cm plants were harvested. Only plants which first internode was at least 5cm long were selected. Stems were surface sterylised and a 1.5cm sample located 2cm away from the rosette was separated for our experiments. The samples were rapidly embedded in OCT and frozen until used. |
---|
Additional Organism Information:
| |
---|
Sample Description | 15cm plants with a first internode at least 5cm long. |
---|
Other Information:
| |
---|
ecotype_habitat | |
---|
Timecourse start procedure | Start point was set when plant were collected. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Agusti_2dNAA_SS1_SLD | | |
|
|
|
Tissue:
| Stem starch sheath + interfascicular fibers. |
---|
in vivo Treatment:
| Stems from 15cm plants were harvested. Only plants which first internode was at least 5cm long were selected. Stems were surface sterylised and a 1.5cm sample located 2cm away from the rosette was separated for our experiments. The samples were then incubated with NAA in a MS split-plate. The samples were then rapidly embedded in OCT and frozen until used. |
---|
Additional Organism Information:
| |
---|
Sample Description | 15cm plants with a first internode at least 5cm long. |
---|
Other Information:
| |
---|
ecotype_habitat | |
---|
Timecourse start procedure | Start point was set when plant were collected. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Agusti_2dNAA_SS2_SLD | | |
|
|
|
Tissue:
| Stem starch sheath + interfascicular fibers. |
---|
in vivo Treatment:
| Stems from 15cm plants were harvested. Only plants which first internode was at least 5cm long were selected. Stems were surface sterylised and a 1.5cm sample located 2cm away from the rosette was separated for our experiments. The samples were then incubated with NAA in a MS split-plate. The samples were then rapidly embedded in OCT and frozen until used. |
---|
Additional Organism Information:
| |
---|
Sample Description | 15cm plants with a first internode at least 5cm long. |
---|
Other Information:
| |
---|
ecotype_habitat | |
---|
Timecourse start procedure | Start point was set when plant were collected. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Agusti_2dNAA_SS3_SLD | | |
|
|
|
Tissue:
| Stem starch sheath + interfascicular fibers. |
---|
in vivo Treatment:
| Stems from 15cm plants were harvested. Only plants which first internode was at least 5cm long were selected. Stems were surface sterylised and a 1.5cm sample located 2cm away from the rosette was separated for our experiments. The samples were then incubated with NAA in a MS split-plate. The samples were then rapidly embedded in OCT and frozen until used. |
---|
Additional Organism Information:
| |
---|
Sample Description | 15cm plants with a first internode at least 5cm long. |
---|
Other Information:
| |
---|
ecotype_habitat | |
---|
Timecourse start procedure | Start point was set when plant were collected. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Agusti_2dNAA_C1_SLD | | |
|
|
|
Tissue:
| Stem except stach sheath layer and vascular bundles. |
---|
in vivo Treatment:
| Stems from 15cm plants were harvested. Only plants which first internode was at least 5cm long were selected. Stems were surface sterylised and a 1.5cm sample located 2cm away from the rosette was separated for our experiments. The samples were then incubated with NAA in a MS split-plate. The samples were then rapidly embedded in OCT and frozen until used. |
---|
Additional Organism Information:
| |
---|
Sample Description | 15cm plants with a first internode at least 5cm long. |
---|
Other Information:
| |
---|
ecotype_habitat | |
---|
Timecourse start procedure | Start point was set when plant were collected. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Agusti_2dNAA_C2_SLD | | |
|
|
|
Tissue:
| Stem except stach sheath layer and vascular bundles. |
---|
in vivo Treatment:
| Stems from 15cm plants were harvested. Only plants which first internode was at least 5cm long were selected. Stems were surface sterylised and a 1.5cm sample located 2cm away from the rosette was separated for our experiments. The samples were then incubated with NAA in a MS split-plate. The samples were then rapidly embedded in OCT and frozen until used. |
---|
Additional Organism Information:
| |
---|
Sample Description | 15cm plants with a first internode at least 5cm long. |
---|
Other Information:
| |
---|
ecotype_habitat | |
---|
Timecourse start procedure | Start point was set when plant were collected. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Agusti_2dNAA_C3_SLD | | |
|
|
|
Tissue:
| Stem except stach sheath layer and vascular bundles. |
---|
in vivo Treatment:
| Stems from 15cm plants were harvested. Only plants which first internode was at least 5cm long were selected. Stems were surface sterylised and a 1.5cm sample located 2cm away from the rosette was separated for our experiments. The samples were then incubated with NAA in a MS split-plate. The samples were then rapidly embedded in OCT and frozen until used. |
---|
Additional Organism Information:
| |
---|
Sample Description | 15cm plants with a first internode at least 5cm long. |
---|
Other Information:
| |
---|
ecotype_habitat | |
---|
Timecourse start procedure | Start point was set when plant were collected. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Agusti_2dMS_SS1_SLD | | |
|
|
|
Tissue:
| Starch sheath + interfascicular fibers |
---|
in vivo Treatment:
| Stems from 15cm plants were harvested. Only plants which first internode was at least 5cm long were selected. Stems were surface sterylised and a 1.5cm sample located 2cm away from the rosette was separated for our experiments. The samples were then incubated in a MS split-plate. The samples were then rapidly embedded in OCT and frozen until used. |
---|
Additional Organism Information:
| |
---|
Sample Description | 15cm plants with a first internode at least 5cm long. |
---|
Other Information:
| |
---|
ecotype_habitat | |
---|
Timecourse start procedure | Start point was set when plant were collected. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Agusti_2dMS_SS2_SLD | | |
|
|
|
Tissue:
| Starch sheath + interfascicular fibers |
---|
in vivo Treatment:
| Stems from 15cm plants were harvested. Only plants which first internode was at least 5cm long were selected. Stems were surface sterylised and a 1.5cm sample located 2cm away from the rosette was separated for our experiments. The samples were then incubated in a MS split-plate. The samples were then rapidly embedded in OCT and frozen until used. |
---|
Additional Organism Information:
| |
---|
Sample Description | 15cm plants with a first internode at least 5cm long. |
---|
Other Information:
| |
---|
ecotype_habitat | |
---|
Timecourse start procedure | Start point was set when plant were collected. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Agusti_2dMS_SS3_SLD | | |
|
|
|
Tissue:
| Starch sheath + interfascicular fibers |
---|
in vivo Treatment:
| Stems from 15cm plants were harvested. Only plants which first internode was at least 5cm long were selected. Stems were surface sterylised and a 1.5cm sample located 2cm away from the rosette was separated for our experiments. The samples were then incubated in a MS split-plate. The samples were then rapidly embedded in OCT and frozen until used. |
---|
Additional Organism Information:
| |
---|
Sample Description | 15cm plants with a first internode at least 5cm long. |
---|
Other Information:
| |
---|
ecotype_habitat | |
---|
Timecourse start procedure | Start point was set when plant were collected. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Agusti_5dNAA_CAM1_SLD | | |
|
|
|
Tissue:
| Starch sheath + interfascicular fibers |
---|
in vivo Treatment:
| Stems from 15cm plants were harvested. Only plants which first internode was at least 5cm long were selected. Stems were surface sterylised and a 1.5cm sample located 2cm away from the rosette was separated for our experiments. The samples were then incubated with NAA in a MS split-plate. The samples were then rapidly embedded in OCT and frozen until used. |
---|
Additional Organism Information:
| |
---|
Sample Description | 15cm plants with a first internode at least 5cm long. |
---|
Other Information:
| |
---|
ecotype_habitat | |
---|
Timecourse start procedure | Start point was set when plant were collected. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Agusti_5dNAA_CAM2_SLD | | |
|
|
|
Tissue:
| Starch sheath + interfascicular fibers |
---|
in vivo Treatment:
| Stems from 15cm plants were harvested. Only plants which first internode was at least 5cm long were selected. Stems were surface sterylised and a 1.5cm sample located 2cm away from the rosette was separated for our experiments. The samples were then incubated with NAA in a MS split-plate. The samples were then rapidly embedded in OCT and frozen until used. |
---|
Additional Organism Information:
| |
---|
Sample Description | 15cm plants with a first internode at least 5cm long. |
---|
Other Information:
| |
---|
ecotype_habitat | |
---|
Timecourse start procedure | Start point was set when plant were collected. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Agusti_5dNAA_CAM3_SLD | | |
|
|
|
Tissue:
| Starch sheath + interfascicular fibers |
---|
in vivo Treatment:
| Stems from 15cm plants were harvested. Only plants which first internode was at least 5cm long were selected. Stems were surface sterylised and a 1.5cm sample located 2cm away from the rosette was separated for our experiments. The samples were then incubated with NAA in a MS split-plate. The samples were then rapidly embedded in OCT and frozen until used. |
---|
Additional Organism Information:
| |
---|
Sample Description | 15cm plants with a first internode at least 5cm long. |
---|
Other Information:
| |
---|
ecotype_habitat | |
---|
Timecourse start procedure | Start point was set when plant were collected. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Agusti_5dNAA_C1_SLD | | |
|
|
|
Tissue:
| Stem except stach sheath layer and vascular bundles. |
---|
in vivo Treatment:
| Stems from 15cm plants were harvested. Only plants which first internode was at least 5cm long were selected. Stems were surface sterylised and a 1.5cm sample located 2cm away from the rosette was separated for our experiments. The samples were then incubated with NAA in a MS split-plate. The samples were then rapidly embedded in OCT and frozen until used. |
---|
Additional Organism Information:
| |
---|
Sample Description | 15cm plants with a first internode at least 5cm long. |
---|
Other Information:
| |
---|
ecotype_habitat | |
---|
Timecourse start procedure | Start point was set when plant were collected. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Agusti_5dNAA_C2_SLD | | |
|
|
|
Tissue:
| Stem except stach sheath layer and vascular bundles. |
---|
in vivo Treatment:
| Stems from 15cm plants were harvested. Only plants which first internode was at least 5cm long were selected. Stems were surface sterylised and a 1.5cm sample located 2cm away from the rosette was separated for our experiments. The samples were then incubated with NAA in a MS split-plate. The samples were then rapidly embedded in OCT and frozen until used. |
---|
Additional Organism Information:
| |
---|
Sample Description | 15cm plants with a first internode at least 5cm long. |
---|
Other Information:
| |
---|
ecotype_habitat | |
---|
Timecourse start procedure | Start point was set when plant were collected. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Agusti_5dNAA_C3_SLD | | |
|
|
|
Tissue:
| Stem except stach sheath layer and vascular bundles. |
---|
in vivo Treatment:
| Stems from 15cm plants were harvested. Only plants which first internode was at least 5cm long were selected. Stems were surface sterylised and a 1.5cm sample located 2cm away from the rosette was separated for our experiments. The samples were then incubated with NAA in a MS split-plate. The samples were then rapidly embedded in OCT and frozen until used. |
---|
Additional Organism Information:
| |
---|
Sample Description | 15cm plants with a first internode at least 5cm long. |
---|
Other Information:
| |
---|
ecotype_habitat | |
---|
Timecourse start procedure | Start point was set when plant were collected. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Agusti_5dMS_SS1_SLD | | |
|
|
|
Tissue:
| Starch sheath + interfascicular fibers |
---|
in vivo Treatment:
| Stems from 15cm plants were harvested. Only plants which first internode was at least 5cm long were selected. Stems were surface sterylised and a 1.5cm sample located 2cm away from the rosette was separated for our experiments. The samples were then incubated in a MS split-plate. The samples were then rapidly embedded in OCT and frozen until used. |
---|
Additional Organism Information:
| |
---|
Sample Description | 15cm plants with a first internode at least 5cm long. |
---|
Other Information:
| |
---|
ecotype_habitat | |
---|
Timecourse start procedure | Start point was set when plant were collected. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Agusti_5dMS_SS2_SLD | | |
|
|
|
Tissue:
| Starch sheath + interfascicular fibers |
---|
in vivo Treatment:
| Stems from 15cm plants were harvested. Only plants which first internode was at least 5cm long were selected. Stems were surface sterylised and a 1.5cm sample located 2cm away from the rosette was separated for our experiments. The samples were then incubated in a MS split-plate. The samples were then rapidly embedded in OCT and frozen until used. |
---|
Additional Organism Information:
| |
---|
Sample Description | 15cm plants with a first internode at least 5cm long. |
---|
Other Information:
| |
---|
ecotype_habitat | |
---|
Timecourse start procedure | Start point was set when plant were collected. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Agusti_5dMS_SS3_SLD | | |
|
|
|
Tissue:
| Starch sheath + interfascicular fibers |
---|
in vivo Treatment:
| Stems from 15cm plants were harvested. Only plants which first internode was at least 5cm long were selected. Stems were surface sterylised and a 1.5cm sample located 2cm away from the rosette was separated for our experiments. The samples were then incubated in a MS split-plate. The samples were then rapidly embedded in OCT and frozen until used. |
---|
Additional Organism Information:
| |
---|
Sample Description | 15cm plants with a first internode at least 5cm long. |
---|
Other Information:
| |
---|
ecotype_habitat | |
---|
Timecourse start procedure | Start point was set when plant were collected. |
---|
|
Protocols for BioSource 1 |
|
|
|
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