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Experiment: Inhibitor of auxin response LI5
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-484
LI5 is an inhibitor of auxin response identified in chemical genetics screen for lateral root emergence inhibitors. Chemicals identified in this screen blocked the auxin induction of LAX3 in the outer tissues (Swarup et al, Nat Cell Biol, 2008). We observed that LI5 affected many other auxin responsive genes (unpublished data, November 2008). Our aim is to identified the overall effects on auxin response using the Arabidopsis thaliana chip Ath1. 3 days old Col-0 Arabidopsis seedlings, germinated on NPA 10uM, were treated for 6 hours with [DMSO, LI5 (5uM), NAA (10uM) or LI5 (5uM) + NAA (10uM)], the shoot and root tip removed and RNA was extracted from the mature part of the root.
About the ExperimenterName: | Mr. Antoine Larrieu |
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Head of Lab Name: | Prof Malcolm Bennett |
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Lab:
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Address: | University of Nottingham Sutton Bonington Campus School of Biosciences Plant Sciences
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Postcode:
| LE12 5RD |
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Country:
| United Kingdom |
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| Telephone Number:
| 00441159516377 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| compound_treatment_design |
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Number of Slides: | 12 |
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| Experimental Parameters:
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parameter | compound_based_treatment |
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Quality Control Measures Taken:
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Larrieu: Inhibitor of auxin response LI5_genome
Slide: Larrieu_1-1_mock-treated_Rep1_ATH1 | | |
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Tissue:
| Mature root |
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in vivo Treatment:
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Treatment | 5 days after sowing, seedlings were transferred for 6 hours to agar plates containing 1/2 strength MS (Murashige and Skoog) salts (pH 5.7), with 1% sucrose and 0.035% DMSO. DMSO was added to the medium after autoclaving |
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Additional Organism Information:
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Sample description | At the end of the treatment, shoots and root tips were removed and the mature part of the root used for RNA extraction. |
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Separation Technique:
| Shoot and root tip were cut off using a razor blade. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Larrieu_1-2_mock-treated_Rep2_ATH1 | | |
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Tissue:
| Mature root |
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in vivo Treatment:
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Treatment | 5 days after sowing, seedlings were transferred for 6 hours to agar plates containing 1/2 strength MS (Murashige and Skoog) salts (pH 5.7), with 1% sucrose and 0.035% DMSO. DMSO was added to the medium after autoclaving |
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Additional Organism Information:
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Sample description | At the end of the treatment, shoots and root tips were removed and the mature part of the root used for RNA extraction. |
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Separation Technique:
| Shoot and root tip were cut off using a razor blade. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Larrieu_1-3_mock-treated_Rep3_ATH1 | | |
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Tissue:
| Mature root |
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in vivo Treatment:
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Treatment | 5 days after sowing, seedlings were transferred for 6 hours to agar plates containing 1/2 strength MS (Murashige and Skoog) salts (pH 5.7), with 1% sucrose and 0.035% DMSO. DMSO was added to the medium after autoclaving |
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Additional Organism Information:
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Sample description | At the end of the treatment, shoots and root tips were removed and the mature part of the root used for RNA extraction. |
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Separation Technique:
| Shoot and root tip were cut off using a razor blade. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Larrieu_1-4_LI5-treated_Rep1_ATH1 | | |
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Tissue:
| Mature root |
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in vivo Treatment:
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Treatment | 5 days after sowing, seedlings were transferred for 6 hours to agar plates containing 1/2 strength MS (Murashige and Skoog) salts (pH 5.7), with 1% sucrose and 5uM LI5 (LI5 made in house, diluted in DMSO). DMSO final concentration is 0.035%. LI5 and DMSO were added to the medium after autoclaving |
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Additional Organism Information:
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Sample description | At the end of the treatment, shoots and root tips were removed and the mature part of the root used for RNA extraction. |
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Separation Technique:
| Shoot and root tip were cut off using a razor blade. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Larrieu_1-5_LI5-treated_Rep2_ATH1 | | |
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Tissue:
| Mature root |
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in vivo Treatment:
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Treatment | 5 days after sowing, seedlings were transferred for 6 hours to agar plates containing 1/2 strength MS (Murashige and Skoog) salts (pH 5.7), with 1% sucrose and 5uM LI5 (LI5 made in house, diluted in DMSO). DMSO final concentration is 0.035%. LI5 and DMSO were added to the medium after autoclaving |
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Additional Organism Information:
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Sample description | At the end of the treatment, shoots and root tips were removed and the mature part of the root used for RNA extraction. |
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Separation Technique:
| Shoot and root tip were cut off using a razor blade. |
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Other Information:
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|
Protocols for BioSource 1 |
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Slide: Larrieu_1-6_LI5-treated_Rep3_ATH1 | | |
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Tissue:
| Mature root |
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in vivo Treatment:
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Treatment | 5 days after sowing, seedlings were transferred for 6 hours to agar plates containing 1/2 strength MS (Murashige and Skoog) salts (pH 5.7), with 1% sucrose and 5uM LI5 (LI5 made in house, diluted in DMSO). DMSO final concentration is 0.035%. LI5 and DMSO were added to the medium after autoclaving |
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Additional Organism Information:
| |
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Sample description | At the end of the treatment, shoots and root tips were removed and the mature part of the root used for RNA extraction. |
---|
Separation Technique:
| Shoot and root tip were cut off using a razor blade. |
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Other Information:
| |
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|
Protocols for BioSource 1 |
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Slide: Larrieu_1-7_Auxin_treated_Rep1_ATH1 | | |
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Tissue:
| Mature root |
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in vivo Treatment:
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Treatment | 5 days after sowing, seedlings were transferred for 6 hours to agar plates containing 1/2 strength MS (Murashige and Skoog) salts (pH 5.7), with 1% sucrose and 10uM NAA (Sigma, UK). NAA was diluted in DMSO. DMSO final concentration was 0.035%. NAA and DMSO were added to the medium after autoclaving. |
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Additional Organism Information:
| |
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Sample description | At the end of the treatment, shoots and root tips were removed and the mature part of the root used for RNA extraction. |
---|
Separation Technique:
| Shoot and root tip were cut off using a razor blade. |
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Other Information:
| |
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|
Protocols for BioSource 1 |
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Slide: Larrieu_1-8_Auxin_treated_Rep2_ATH1 | | |
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Tissue:
| Mature root |
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in vivo Treatment:
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Treatment | 5 days after sowing, seedlings were transferred for 6 hours to agar plates containing 1/2 strength MS (Murashige and Skoog) salts (pH 5.7), with 1% sucrose and 10uM NAA (Sigma, UK). NAA was diluted in DMSO. DMSO final concentration was 0.035%. NAA and DMSO were added to the medium after autoclaving. |
---|
Additional Organism Information:
| |
---|
Sample description | At the end of the treatment, shoots and root tips were removed and the mature part of the root used for RNA extraction. |
---|
Separation Technique:
| Shoot and root tip were cut off using a razor blade. |
---|
Other Information:
| |
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|
Protocols for BioSource 1 |
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Slide: Larrieu_1-9_Auxin_treated_Rep3_ATH1 | | |
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Tissue:
| Mature root |
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in vivo Treatment:
| |
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Treatment | 5 days after sowing, seedlings were transferred for 6 hours to agar plates containing 1/2 strength MS (Murashige and Skoog) salts (pH 5.7), with 1% sucrose and 10uM NAA (Sigma, UK). NAA was diluted in DMSO. DMSO final concentration was 0.035%. NAA and DMSO were added to the medium after autoclaving. |
---|
Additional Organism Information:
| |
---|
Sample description | At the end of the treatment, shoots and root tips were removed and the mature part of the root used for RNA extraction. |
---|
Separation Technique:
| Shoot and root tip were cut off using a razor blade. |
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Other Information:
| |
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|
Protocols for BioSource 1 |
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Slide: Larrieu_1-10_Auxin-LI5-treated_Rep1_ATH1 | | |
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Tissue:
| Mature root |
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in vivo Treatment:
| |
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Treatment | 5 days after sowing, seedlings were transferred for 6 hours to agar plates containing 1/2 strength MS (Murashige and Skoog) salts (pH 5.7), with 1% sucrose, 10uM NAA (Sigma, UK) and 5uM LI5 (made in house, diluted in DMSO). NAA was diluted in DMSO. DMSO final concentration was 0.035%. NAA and LI5 were added to the medium after autoclaving. |
---|
Additional Organism Information:
| |
---|
Sample description | At the end of the treatment, shoots and root tips were removed and the mature part of the root used for RNA extraction |
---|
Separation Technique:
| Shoot and root tip were cut off using a razor blade. |
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Other Information:
| |
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|
Protocols for BioSource 1 |
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Slide: Larrieu_1-11_Auxin-LI5-treated_Rep2_ATH1 | | |
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Tissue:
| Mature root |
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in vivo Treatment:
| |
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Treatment | 5 days after sowing, seedlings were transferred for 6 hours to agar plates containing 1/2 strength MS (Murashige and Skoog) salts (pH 5.7), with 1% sucrose, 10uM NAA (Sigma, UK) and 5uM LI5 (made in house, diluted in DMSO). NAA was diluted in DMSO. DMSO final concentration was 0.035%. NAA and LI5 were added to the medium after autoclaving. |
---|
Additional Organism Information:
| |
---|
Sample description | At the end of the treatment, shoots and root tips were removed and the mature part of the root used for RNA extraction |
---|
Separation Technique:
| Shoot and root tip were cut off using a razor blade. |
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Other Information:
| |
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|
Protocols for BioSource 1 |
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Slide: Larrieu_1-12_Auxin-LI5-treated_Rep3_ATH1 | | |
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Tissue:
| Mature root |
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in vivo Treatment:
| |
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Treatment | 5 days after sowing, seedlings were transferred for 6 hours to agar plates containing 1/2 strength MS (Murashige and Skoog) salts (pH 5.7), with 1% sucrose, 10uM NAA (Sigma, UK) and 5uM LI5 (made in house, diluted in DMSO). NAA was diluted in DMSO. DMSO final concentration was 0.035%. NAA and LI5 were added to the medium after autoclaving. |
---|
Additional Organism Information:
| |
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Sample description | At the end of the treatment, shoots and root tips were removed and the mature part of the root used for RNA extraction |
---|
Separation Technique:
| Shoot and root tip were cut off using a razor blade. |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
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Problems? Comments? Suggestions? Contact the Affymetrix Team