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Experiment: Microarray analysis of Arabidopsis plants inoculated with HopAO1 mutants of Pst DC3000
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-417
This experiment was performed to identify gene expression changes in wild type (Col-0) Arabidopsis plants after inoculation with Pseudomonas syringae pv. tomato (Pst) strain DC3000 or a mutant derivative lacking the type III effector tyrosine phosphatase HopAO1. Col-0 Arabidopsis plants were inoculated by vacuum-infiltration with suspensions of Pst DC3000 or the deltaHopAO1 mutant at 10e8 cfu/ml or with a mock inoculum of sterile water. Inoculated leaf tissues were harvested 7 hours after infiltration and used for RNA isolation and subsequent microarray analysis using Affymetrix ATH1 genechips.
About the ExperimenterName: | Dr. William Underwood |
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Head of Lab Name: | Dr. Sheng Yang He |
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Lab:
| DOE Plant Research Laboratory |
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Institute:
| Michigan State University |
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Address: | DOE Plant Research Laboratory Michigan State University East Lansing, MI
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Postcode:
| 48824 |
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Country:
| USA |
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| Telephone Number:
| 5173539182 |
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are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| pathogenicity_design |
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Number of Slides: | 9 |
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| Experimental Parameters:
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parameter | infect |
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Quality Control Measures Taken:
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Underwood: Microarray analysis of Arabidopsis plants inoculated with HopAO1 mutants of Pst DC3000_genome
Slide: Underwood_3-1_Col0-PstDC3000-inocu_Rep1_ATH1 | | |
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Tissue:
| Leaves |
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in vivo Treatment:
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treatment | Plants were inoculated by vacuum-infiltration with a 1x10e8 cfu/ml suspension of Pst DC3000 in sterile water containing .004% Silwet L-77. Leaf tissue from at least 15 plants was collected 7 hours after inoculation for RNA isolation. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Underwood_3-2_Col0-PstDC3000-inocu_Rep2_ATH1 | | |
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Tissue:
| Leaves |
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in vivo Treatment:
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treatment | Plants were inoculated by vacuum-infiltration with a 1x10e8 cfu/ml suspension of Pst DC3000 in sterile water containing .004% Silwet L-77. Leaf tissue from at least 15 plants was collected 7 hours after inoculation for RNA isolation. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Underwood_3-3_Col0-PstDC3000-inocu_Rep3_ATH1 | | |
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Tissue:
| Leaves |
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in vivo Treatment:
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treatment | Plants were inoculated by vacuum-infiltration with a 1x10e8 cfu/ml suspension of Pst DC3000 in sterile water containing .004% Silwet L-77. Leaf tissue from at least 15 plants was collected 7 hours after inoculation for RNA isolation. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Underwood_3-4_Col0-PstDC3000-deltaHopAO1-inocu_Rep1_ATH1 | | |
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Tissue:
| Leaves |
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in vivo Treatment:
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treatment | Plants were inoculated by vacuum-infiltration with a 1x10e8 cfu/ml suspension of the Pst DC3000 deltaHopAO1 mutant in sterile water containing .004% Silwet L-77. Leaf tissue from at least 15 plants was collected 7 hours after inoculation for RNA isolation. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Underwood_3-5_Col0-PstDC3000-deltaHopAO1-inocu_Rep2_ATH1 | | |
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Tissue:
| Leaves |
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in vivo Treatment:
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treatment | Plants were inoculated by vacuum-infiltration with a 1x10e8 cfu/ml suspension of the Pst DC3000 deltaHopAO1 mutant in sterile water containing .004% Silwet L-77. Leaf tissue from at least 15 plants was collected 7 hours after inoculation for RNA isolation. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Underwood_3-6_Col0-PstDC3000-deltaHopAO1-inocu_Rep3_ATH1 | | |
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Tissue:
| Leaves |
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in vivo Treatment:
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treatment | Plants were inoculated by vacuum-infiltration with a 1x10e8 cfu/ml suspension of the Pst DC3000 deltaHopAO1 mutant in sterile water containing .004% Silwet L-77. Leaf tissue from at least 15 plants was collected 7 hours after inoculation for RNA isolation. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Underwood_3-7_Col0-H2O-mock-inocu_Rep1_ATH1 | | |
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Tissue:
| Leaves |
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in vivo Treatment:
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treatment | Plants were inoculated by vacuum-infiltration with a mock inoculum of sterile water containing .004% Silwet L-77. Leaf tissue from at least 15 plants was collected 7 hours after inoculation for RNA isolation. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Underwood_3-8_Col0-H2O-mock-inocu_Rep2_ATH1 | | |
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Tissue:
| Leaves |
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in vivo Treatment:
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treatment | Plants were inoculated by vacuum-infiltration with a mock inoculum of sterile water containing .004% Silwet L-77. Leaf tissue from at least 15 plants was collected 7 hours after inoculation for RNA isolation. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Underwood_3-9_Col0-H2O-mock-inocu_Rep3_ATH1 | | |
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Tissue:
| Leaves |
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in vivo Treatment:
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treatment | Plants were inoculated by vacuum-infiltration with a mock inoculum of sterile water containing .004% Silwet L-77. Leaf tissue from at least 15 plants was collected 7 hours after inoculation for RNA isolation. |
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Other Information:
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Protocols for BioSource 1 |
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Problems? Comments? Suggestions? Contact the Affymetrix Team