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Experiment: Gibberellin responsive genes in Arabidopsis
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-423
The aim of the experiment was to identify early gibberellin (GA) responsive genes in the roots of an Arabidopsis GA deficient mutant. The GA deficient mutant used in this study is a transgenic line overexpressing the PcGA2ox1 gene. This mutant has an identical phenotype to ga1-3, but it does not require exogenous GA treatment for germination. Seeds were germinated on 1xMS + 1% (w/v) sucrose plates containing 0.7% gelrite, and grown under continous light. The plates were orientated vertically. After six days growth the plates were transferred to acclimatisation baths containing 1 x MS liquid media so that the roots were submerged. After 24 hours acclimatisation plates were treated with or without 2uM GA4 for 0, 30, 60 and 180 minutes. Root tips (up to the end of the elongation zone) were harvested from these plants (approx 150 per sample), giving a total of 7 experimental samples (1: untreated, 2: 30 mins GA4, 3: 30 mins untreated, 4: 60 mins GA4, 5: 60 mins untreated, 6: 180 mins GA4, 7: 180 mins untreated. Three biological replicates were performed giving a total of 21 root samples. Total RNA was isolated from the roots using the QIAGEN RNeasy method.
About the ExperimenterName: | Dr Steve Thomas |
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Head of Lab Name: | Dr Steve Thomas |
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Lab:
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Address: | Rothamsted Research Harpenden Herts.
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Postcode:
| AL5 2JQ |
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Country:
| UK |
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| Telephone Number:
| 01582763133 |
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Fax Number:
| 01582763010 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| compound_treatment_design;time_series_design; |
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Number of Slides: | 21 |
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| Experimental Parameters:
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parameter | compound_based_treatment |
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parameter | timepoint |
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Quality Control Measures Taken:
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Thomas: Gibberellin responsive genes in Arabidopsis_genome
Slide: Thomas_1-21_GA4-180mins_Rep3_ATH1 | | |
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Tissue:
| root tips |
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in vivo Treatment:
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Treatment | 2uM GA4 |
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Additional Organism Information:
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Sample description | Plants were 7 day-old Arabidopsis seedlings grown on MS plates. The root tips were used to prepare the total RNA sample. The tip included the elongation zone, but not the differentiation zone. |
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allele | PcGA2ox1 (EMBL accession number: At132438) |
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Separation Technique:
| root tip was removed by cutting it off with a razor blade |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Thomas_1-20_GA4-60mins_Rep3_ATH1 | | |
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Tissue:
| root tips |
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in vivo Treatment:
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Treatment | 2uM GA4 |
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Additional Organism Information:
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Sample description | Plants were 7 day-old Arabidopsis seedlings grown on MS plates. The root tips were used to prepare the total RNA sample. The tip included the elongation zone, but not the differentiation zone. |
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allele | PcGA2ox1 (EMBL accession number: At132438) |
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Separation Technique:
| root tip was removed by cutting it off with a razor blade |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Thomas_1-19_GA4-30mins_Rep3_ATH1 | | |
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Tissue:
| root tips |
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in vivo Treatment:
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Treatment | 2uM GA4 |
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Additional Organism Information:
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Sample description | Plants were 7 day-old Arabidopsis seedlings grown on MS plates. The root tips were used to prepare the total RNA sample. The tip included the elongation zone, but not the differentiation zone. |
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allele | PcGA2ox1 (EMBL accession number: At132438) |
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Separation Technique:
| root tip was removed by cutting it off with a razor blade |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Thomas_1-18_Control-180mins_Rep3_ATH1 | | |
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Tissue:
| root tips |
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Additional Organism Information:
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Sample description | Plants were 7 day-old Arabidopsis seedlings grown on MS plates. The root tips were used to prepare the total RNA sample. The tip included the elongation zone, but not the differentiation zone. |
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allele | PcGA2ox1 (EMBL accession number: At132438) |
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Separation Technique:
| root tip was removed by cutting it off with a razor blade |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Thomas_1-17_Control-60mins_Rep3_ATH1 | | |
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Tissue:
| root tips |
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Additional Organism Information:
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Sample description | Plants were 7 day-old Arabidopsis seedlings grown on MS plates. The root tips were used to prepare the total RNA sample. The tip included the elongation zone, but not the differentiation zone. |
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allele | PcGA2ox1 (EMBL accession number: At132438) |
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Separation Technique:
| root tip was removed by cutting it off with a razor blade |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Thomas_1-16_Control-30mins_Rep3_ATH1 | | |
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Tissue:
| root tips |
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Additional Organism Information:
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Sample description | Plants were 7 day-old Arabidopsis seedlings grown on MS plates. The root tips were used to prepare the total RNA sample. The tip included the elongation zone, but not the differentiation zone. |
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allele | PcGA2ox1 (EMBL accession number: At132438) |
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Separation Technique:
| root tip was removed by cutting it off with a razor blade |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Thomas_1-15_Untreated-control_Rep3_ATH1 | | |
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Tissue:
| root tips |
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Additional Organism Information:
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Sample description | Plants were 7 day-old Arabidopsis seedlings grown on MS plates. The root tips were used to prepare the total RNA sample. The tip included the elongation zone, but not the differentiation zone. |
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allele | PcGA2ox1 (EMBL accession number: At132438) |
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Separation Technique:
| root tip was removed by cutting it off with a razor blade |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Thomas_1-1_Untreated-control_Rep1_ATH1 | | |
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Tissue:
| root tips |
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Additional Organism Information:
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Sample description | Plants were 7 day-old Arabidopsis seedlings grown on MS plates. The root tips were used to prepare the total RNA sample. The tip included the elongation zone, but not the differentiation zone. |
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allele | PcGA2ox1 (EMBL accession number: At132438) |
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Separation Technique:
| root tip was removed by cutting it off with a razor blade |
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Other Information:
| 7 days after sowing the seeds and transferring to the growth room |
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Protocols for BioSource 1 |
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Slide: Thomas_1-2_Control-30mins_Rep1_ATH1 | | |
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Tissue:
| root tips |
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Additional Organism Information:
| |
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Sample description | Plants were 7 day-old Arabidopsis seedlings grown on MS plates. The root tips were used to prepare the total RNA sample. The tip included the elongation zone, but not the differentiation zone. |
---|
allele | PcGA2ox1 (EMBL accession number: At132438) |
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Separation Technique:
| root tip was removed by cutting it off with a razor blade |
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Other Information:
| the start time was when the gibberellin hormone or water control was applied to the plants |
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Protocols for BioSource 1 |
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Slide: Thomas_1-3_Control-60mins_Rep1_ATH1 | | |
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Tissue:
| root tips |
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Additional Organism Information:
| |
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Sample description | Plants were 7 day-old Arabidopsis seedlings grown on MS plates. The root tips were used to prepare the total RNA sample. The tip included the elongation zone, but not the differentiation zone. |
---|
allele | PcGA2ox1 (EMBL accession number: At132438) |
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Separation Technique:
| root tip was removed by cutting it off with a razor blade |
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Other Information:
| the start time was when the gibberellin hormone or water control was applied to the plants |
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Protocols for BioSource 1 |
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Slide: Thomas_1-4_Control-180mins_Rep1_ATH1 | | |
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Tissue:
| root tips |
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Additional Organism Information:
| |
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Sample description | Plants were 7 day-old Arabidopsis seedlings grown on MS plates. The root tips were used to prepare the total RNA sample. The tip included the elongation zone, but not the differentiation zone. |
---|
allele | PcGA2ox1 (EMBL accession number: At132438) |
---|
Separation Technique:
| root tip was removed by cutting it off with a razor blade |
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Other Information:
| the start time was when the gibberellin hormone or water control was applied to the plants |
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Protocols for BioSource 1 |
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Slide: Thomas_1-5_GA4-30mins_Rep1_ATH1 | | |
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Tissue:
| root tips |
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in vivo Treatment:
| |
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Treatment | 2uM GA4 |
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Additional Organism Information:
| |
---|
Sample description | Plants were 7 day-old Arabidopsis seedlings grown on MS plates. The root tips were used to prepare the total RNA sample. The tip included the elongation zone, but not the differentiation zone. |
---|
allele | PcGA2ox1 (EMBL accession number: At132438) |
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Separation Technique:
| root tip was removed by cutting it off with a razor blade |
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Other Information:
| the start time was when the gibberellin hormone or water control was applied to the plants |
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Protocols for BioSource 1 |
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Slide: Thomas_1-6_GA4-60mins_Rep1_ATH1 | | |
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Tissue:
| root tips |
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in vivo Treatment:
| |
---|
Treatment | 2uM GA4 |
---|
Additional Organism Information:
| |
---|
Sample description | Plants were 7 day-old Arabidopsis seedlings grown on MS plates. The root tips were used to prepare the total RNA sample. The tip included the elongation zone, but not the differentiation zone. |
---|
allele | PcGA2ox1 (EMBL accession number: At132438) |
---|
Separation Technique:
| root tip was removed by cutting it off with a razor blade |
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Other Information:
| the start time was when the gibberellin hormone or water control was applied to the plants |
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|
Protocols for BioSource 1 |
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Slide: Thomas_1-7_GA4-180mins_Rep1_ATH1 | | |
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Tissue:
| root tips |
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in vivo Treatment:
| |
---|
Treatment | 2uM GA4 |
---|
Additional Organism Information:
| |
---|
Sample description | Plants were 7 day-old Arabidopsis seedlings grown on MS plates. The root tips were used to prepare the total RNA sample. The tip included the elongation zone, but not the differentiation zone. |
---|
allele | PcGA2ox1 (EMBL accession number: At132438) |
---|
Separation Technique:
| root tip was removed by cutting it off with a razor blade |
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Other Information:
| the start time was when the gibberellin hormone or water control was applied to the plants |
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|
Protocols for BioSource 1 |
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Slide: Thomas_1-8_Untreated-control_Rep2_ATH1 | | |
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Tissue:
| root tips |
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Additional Organism Information:
| |
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Sample description | Plants were 7 day-old Arabidopsis seedlings grown on MS plates. The root tips were used to prepare the total RNA sample. The tip included the elongation zone, but not the differentiation zone. |
---|
allele | PcGA2ox1 (EMBL accession number: At132438) |
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Separation Technique:
| root tip was removed by cutting it off with a razor blade |
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Other Information:
| |
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|
Protocols for BioSource 1 |
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Slide: Thomas_1-9_Control-30mins_Rep2_ATH1 | | |
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Tissue:
| root tips |
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Additional Organism Information:
| |
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Sample description | Plants were 7 day-old Arabidopsis seedlings grown on MS plates. The root tips were used to prepare the total RNA sample. The tip included the elongation zone, but not the differentiation zone. |
---|
allele | PcGA2ox1 (EMBL accession number: At132438) |
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Separation Technique:
| root tip was removed by cutting it off with a razor blade |
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Other Information:
| |
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|
Protocols for BioSource 1 |
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Slide: Thomas_1-10_Control-60mins_Rep2_ATH1 | | |
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Tissue:
| root tips |
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Additional Organism Information:
| |
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Sample description | Plants were 7 day-old Arabidopsis seedlings grown on MS plates. The root tips were used to prepare the total RNA sample. The tip included the elongation zone, but not the differentiation zone. |
---|
allele | PcGA2ox1 (EMBL accession number: At132438) |
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Separation Technique:
| root tip was removed by cutting it off with a razor blade |
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Other Information:
| |
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|
Protocols for BioSource 1 |
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Slide: Thomas_1-11_Control-180mins_Rep2_ATH1 | | |
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Tissue:
| root tips |
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Additional Organism Information:
| |
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Sample description | Plants were 7 day-old Arabidopsis seedlings grown on MS plates. The root tips were used to prepare the total RNA sample. The tip included the elongation zone, but not the differentiation zone. |
---|
allele | PcGA2ox1 (EMBL accession number: At132438) |
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Separation Technique:
| root tip was removed by cutting it off with a razor blade |
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Other Information:
| |
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|
Protocols for BioSource 1 |
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Slide: Thomas_1-12_GA4-30mins_Rep2_ATH1 | | |
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Tissue:
| root tips |
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in vivo Treatment:
| |
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Treatment | 2uM GA4 |
---|
Additional Organism Information:
| |
---|
Sample description | Plants were 7 day-old Arabidopsis seedlings grown on MS plates. The root tips were used to prepare the total RNA sample. The tip included the elongation zone, but not the differentiation zone. |
---|
allele | PcGA2ox1 (EMBL accession number: At132438) |
---|
Separation Technique:
| root tip was removed by cutting it off with a razor blade |
---|
Other Information:
| |
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|
Protocols for BioSource 1 |
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Slide: Thomas_1-13_GA4-60mins_Rep2_ATH1 | | |
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Tissue:
| root tips |
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in vivo Treatment:
| |
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Treatment | 2uM GA4 |
---|
Additional Organism Information:
| |
---|
Sample description | Plants were 7 day-old Arabidopsis seedlings grown on MS plates. The root tips were used to prepare the total RNA sample. The tip included the elongation zone, but not the differentiation zone. |
---|
allele | PcGA2ox1 (EMBL accession number: At132438) |
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Separation Technique:
| root tip was removed by cutting it off with a razor blade |
---|
Other Information:
| |
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|
Protocols for BioSource 1 |
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Slide: Thomas_1-14_GA4-180mins_Rep2_ATH1 | | |
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Tissue:
| root tips |
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in vivo Treatment:
| |
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Treatment | 2uM GA4 |
---|
Additional Organism Information:
| |
---|
Sample description | Plants were 7 day-old Arabidopsis seedlings grown on MS plates. The root tips were used to prepare the total RNA sample. The tip included the elongation zone, but not the differentiation zone. |
---|
allele | PcGA2ox1 (EMBL accession number: At132438) |
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Separation Technique:
| root tip was removed by cutting it off with a razor blade |
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Other Information:
| |
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|
Protocols for BioSource 1 |
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