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Experiment: Gibberellin and ethylene cross-talk at the level of transcriptional regulation in Arabidopsis
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-81
This work is part of an existing collaboration between the two laboratories, funded by the EU (EU-RTN-INTEGA). Both parties will share the cost of this microarray experiment.
Background: We have demonstrated that ethylene-insensitive mutants and wild type(col-0) Arabidopsis plants treated with an ethylene perception inhibitor have increased levels of expression of genes, such as GASA1 and g-TIP, that are thought to be regulated by GA (Vriezen et al, unpublished results). However, this observation was based on an RNA gel blot analysis and therefore limited to few genes.
Aim: To investigate whether plants with decreased ethylene perception are generally hypersensitive to GA or whether this effect is restricted to specific genes. We plan to undertake a complete transcriptome analysis of GA-treated wild type andetr1-1 plants. The aim is to identify genes that are induced directly as a result of the GA treatment, and we will therefore focus on the time window 0-3h.
Tissues to be sampled: Plants will be grown in vitroon MS/2 containing 1% sucrose, pH 5.7, at 22 C,70% RH, under white light (54 PAR) and a photoperiod of 16h light/8h dark. Plants will be treated at 14 days and harvested entirely, i.e. roots and shoots are extracted together.
Experimental set-up: Col-0 and the ethylene-insensitive mutant etr1-1 will be sprayed with 50 microM GA4 in water. GA4 is the major bio-active GA in Arabidopsis. Samples will be taken after 0, 30 min, 1h, and 3h. In order to correct for touch-induced genes a control, which is sprayed with water only and harvested at 1h, will be included for both genotypes. The total number of chips to be hybridized is 10. The time course with 4 data points is preferred to a single time point with 3 repeats, because it will allow us to follow the induction kinetics and identify early response genes. For each timepoint, RNA will be extracted from at least 40 individuals.
About the ExperimenterName: | Liesbeth De Grauwe |
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Head of Lab Name: | Prof.Dr. Dominique Van Der Straeten |
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Lab:
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Institute:
| Ghent University |
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Address: | Ledeganckstraat 35 Department of Genetics Ghent University Ghent
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Postcode:
| B-9000 |
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Country:
| Belgium |
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| Telephone Number:
| +32-9-2645185 |
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Fax Number:
| +32-9-2645333 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| compound_treatment_design |
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Number of Slides: | 18 |
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| Experimental Parameters:
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Parameter | compound_based_treatment |
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parameter | gene_knock_out |
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Quality Control Measures Taken:
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References:
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| Other Information:
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ArrayExpressAccession | E-NASC-20 |
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Slides in this Experiment
Hybridisation Set: De Grauwe_genome_Rep1
Slide: De Grauwe_1-1_water-Col0-0hr_Rep1_ATH1 | | |
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Tissue:
| Whole plant |
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Diseased:
| Normal |
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in vivo Treatment:
| This plant was sprayed with water and immediately harvested |
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Other Information:
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Protocols for BioSource 1 |
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Slide: De Grauwe_1-2_water-Col0-1hr_Rep1_ATH1 | | |
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Tissue:
| Whole plant |
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Diseased:
| Normal |
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in vivo Treatment:
| This plant was sprayed with water and harvested 1hour after treatment |
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Other Information:
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Protocols for BioSource 1 |
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Slide: De Grauwe_1-3_GA4-Col0-30min_Rep1_ATH1 | | |
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Tissue:
| Whole plant |
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Diseased:
| Normal |
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in vivo Treatment:
| This plant was sprayed GA4, which was dissolved in water, and harvested 30min after treatment |
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Other Information:
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Protocols for BioSource 1 |
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Slide: De Grauwe_1-4_GA4-Col0-1hr_Rep1_ATH1 | | |
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Tissue:
| Whole plant |
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Diseased:
| Normal |
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in vivo Treatment:
| This plant was sprayed GA4, which was dissolved in water, and harvested 1hour after treatment |
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Other Information:
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Protocols for BioSource 1 |
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Slide: De Grauwe_1-5_GA4-Col0-3hr_Rep1_ATH1 | | |
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Tissue:
| Whole plant |
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Diseased:
| Normal |
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in vivo Treatment:
| This plant was sprayed GA4, which was dissolved in water, and harvested 3hours after treatment |
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Other Information:
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Protocols for BioSource 1 |
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Slide: De Grauwe_1-6_water-etr1-0hr_Rep1_ATH1 | | |
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Tissue:
| Whole plant |
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Diseased:
| Normal |
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in vivo Treatment:
| This plant was sprayed with water, and immediately harvested |
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Other Information:
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Protocols for BioSource 1 |
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Slide: De Grauwe_1-7_water-etr1-1hr_Rep1_ATH1 | | |
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Tissue:
| Whole plant |
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Diseased:
| Normal |
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in vivo Treatment:
| This plant was sprayed with water, and harvested 1hour after treatment |
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Other Information:
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Protocols for BioSource 1 |
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Slide: De Grauwe_1-8_GA4-etr1-30min_Rep1_ATH1 | | |
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Tissue:
| Whole plant |
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Diseased:
| Normal |
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in vivo Treatment:
| This plant was sprayed with GA4, which was dissolved in water, and harvested 30min. after treatment |
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Other Information:
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Protocols for BioSource 1 |
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Slide: De Grauwe_1-9_GA4-etr1-1hr_Rep1_ATH1 | | |
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Tissue:
| Whole plant |
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Diseased:
| Normal |
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in vivo Treatment:
| This plant was sprayed with GA4, which was dissolved in water, and harvested 1hour after treatment |
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Other Information:
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Protocols for BioSource 1 |
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Slide: De Grauwe_1-10_GA4-etr1-3hr_Rep1_ATH1 | | |
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Tissue:
| Whole plant |
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Diseased:
| Normal |
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in vivo Treatment:
| This plant was sprayed with GA4, which was dissolved in water, and harvested 3hours after treatment |
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Other Information:
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Protocols for BioSource 1 |
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Hybridisation Set: De Grauwe_genome_Rep2
Slide: De Grauwe_1-2_water-Col0-1hr_Rep2_ATH1 | | |
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Tissue:
| Whole plant |
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Diseased:
| Normal |
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in vivo Treatment:
| This plant was sprayed with water and harvested 1hour after treatment |
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Other Information:
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Protocols for BioSource 1 |
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Slide: De Grauwe_1-4_GA4-Col0-1hr_Rep2_ATH1 | | |
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Tissue:
| Whole plant |
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Diseased:
| Normal |
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in vivo Treatment:
| This plant was sprayed GA4, which was dissolved in water, and harvested 1hour after treatment |
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Other Information:
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Protocols for BioSource 1 |
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Slide: De Grauwe_1-7_water-etr1-1hr_Rep2_ATH1 | | |
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Tissue:
| Whole plant |
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Diseased:
| Normal |
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in vivo Treatment:
| This plant was sprayed with water, and harvested 1hour after treatment |
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Other Information:
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Protocols for BioSource 1 |
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Slide: De Grauwe_1-9_GA4-etr1-1hr_Rep2_ATH1 | | |
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Tissue:
| Whole plant |
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Diseased:
| Normal |
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in vivo Treatment:
| This plant was sprayed with GA4, which was dissolved in water, and harvested 1hour after treatment |
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Other Information:
| |
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Protocols for BioSource 1 |
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Hybridisation Set: De Grauwe_genome_Rep3
Slide: De Grauwe_1-2_water-Col0-1hr_Rep3_ATH1 | | |
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Tissue:
| Whole plant |
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Diseased:
| Normal |
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in vivo Treatment:
| This plant was sprayed with water and harvested 1hour after treatment |
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Other Information:
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Protocols for BioSource 1 |
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Slide: De Grauwe_1-4_GA4-Col0-1hr_Rep3_ATH1 | | |
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Tissue:
| Whole plant |
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Diseased:
| Normal |
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in vivo Treatment:
| This plant was sprayed GA4, which was dissolved in water, and harvested 1hour after treatment |
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Other Information:
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Protocols for BioSource 1 |
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Slide: De Grauwe_1-7_water-etr1-1hr_Rep3_ATH1 | | |
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Tissue:
| Whole plant |
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Diseased:
| Normal |
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in vivo Treatment:
| This plant was sprayed with water, and harvested 1hour after treatment |
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Other Information:
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Protocols for BioSource 1 |
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Slide: De Grauwe_1-9_GA4-etr1-1hr_Rep3_ATH1 | | |
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Tissue:
| Whole plant |
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Diseased:
| Normal |
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in vivo Treatment:
| This plant was sprayed with GA4, which was dissolved in water, and harvested 1hour after treatment |
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Other Information:
| |
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|
Protocols for BioSource 1 |
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Problems? Comments? Suggestions? Contact the Affymetrix Team