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Experiment: Interaction Arabidopsis thaliana vs. Ralstonia solanacearum
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-447
Herein, whole genome expression analysis was conducted on rosettes of resistant and susceptible Arabidopsis thaliana accessions challenged with Ralstonia solanacearum. Two disease situations were considered: Col-5 plants inoculated with the virulent GMI1000 strain, as well as Nd-1 plants challenged with the same bacterial strain deleted of the avr PopP2 gene (DeltaPopP2), in both cases developing wilt disease symptoms. In contrast, Nd-1 plants challenged with GMI1000 are fully resistant to the pathogen and served as a control.Samples were harvested at the time of inoculation, as well as at 6, 12, 24 hours -at these times no symptoms were visible-, 5 days after inoculation when the first wilt symptoms became visible (25% of wilted leaves: disease index 1, D1), and finally 8 days after inoculation when 75 % of leaves were completely wilted (disease index 3, D3).
About the ExperimenterName: | Group leader Yves Marco |
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Head of Lab Name: | Director of the institute Pascal Gamas |
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Lab:
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Address: | Laboratoire des Interactions Plantes-Microorganism UMR CNRS-INRA 2594/441 Chemin de borde rouge BP 52627
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Postcode:
| 31326 |
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Country:
| France |
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| Telephone Number:
| 33561285509 |
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Fax Number:
| 33561285061 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| time_series_design;pathogenicity_design |
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Number of Slides: | 34 |
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| Experimental Parameters:
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parameter | infect |
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Quality Control Measures Taken:
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Marco: Interaction Arabidopsis thaliana vs. Ralstonia solanacearum_genome
Slide: Marco_2-1_Col-0H_Rep1_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
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Treatment protocol | Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+8 bacteria per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared. |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
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Sample description | Rosettes were harvested NucleoSpin RNAII kit (Macherey-Nagel, GmbH&Co.KG, Dueren, Germany) before inoculation. |
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Protocols for BioSource 1 |
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Slide: Marco_2-2_Col-0H_Rep2_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
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Treatment protocol | Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+8 bacteria per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared. |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
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Sample description | Rosettes were harvested NucleoSpin RNAII kit (Macherey-Nagel, GmbH&Co.KG, Dueren, Germany) before inoculation. |
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Protocols for BioSource 1 |
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Slide: Marco_2-3_Col-1000-6H_Rep1_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
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Treatment protocol | Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared. |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
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Sample description | 6H post inoculation |
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Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml |
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Protocols for BioSource 1 |
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Slide: Marco_2-4_Col-1000-6H_Rep2_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
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Treatment protocol | Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared. |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
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Sample description | 6H post inoculation |
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Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml |
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Protocols for BioSource 1 |
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Slide: Marco_2-5_Col-1000-12H_Rep1_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
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Treatment protocol | Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared. |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
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Sample description | 12H post inoculation. |
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Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. |
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Protocols for BioSource 1 |
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Slide: Marco_2-6_Col-1000-12H_Rep2_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
| |
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Treatment protocol | Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared. |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
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Sample description | 12H post inoculation. |
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Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. |
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Protocols for BioSource 1 |
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Slide: Marco_2-7_Col-1000-24H_Rep1_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
| |
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Treatment protocol | Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared. |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
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Sample description | 24H post inoculation. |
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Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. |
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Protocols for BioSource 1 |
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Slide: Marco_2-8_Col-1000-24H_Rep2_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
| |
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Treatment protocol | Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared. |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
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Sample description | 24H post inoculation. |
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Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. |
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Protocols for BioSource 1 |
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Slide: Marco_2-9_Col-1000-D1_Rep1_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
| |
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Treatment protocol | Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared. |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
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Sample description | 5 days post inoculation. 25% of wilted leaves: disease index 1, D1. |
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Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. |
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Protocols for BioSource 1 |
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Slide: Marco_2-10_Col-1000-D1_Rep2_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
| |
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Treatment protocol | Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared. |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
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Sample description | 5 days post inoculation. 25% of wilted leaves: disease index 1, D1. |
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Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. |
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Protocols for BioSource 1 |
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Slide: Marco_2-11_Col-1000-D3_Rep1_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
| |
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Treatment protocol | Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared. |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
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Sample description | 8 days post inoculation. 75% of wilted leaves: disease index 3, D3 |
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Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml |
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Protocols for BioSource 1 |
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Slide: Marco_2-12_Col-1000-D3_Rep2_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
| |
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Treatment protocol | Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared. |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
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Sample description | 8 days post inoculation. 75% of wilted leaves: disease index 3, D3 |
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Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml |
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Protocols for BioSource 1 |
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Slide: Marco_2-13_Nd-0H_Rep1_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
| |
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Treatment protocol | Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared. |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
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Sample description | 24H post inoculation. |
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Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml |
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Protocols for BioSource 1 |
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Slide: Marco_2-14_Nd-0H_Rep2_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
| |
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Treatment protocol | Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared. |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
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Sample description | 24H post inoculation. |
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Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml |
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Protocols for BioSource 1 |
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Slide: Marco_2-15_Nd-1000-6H_Rep1_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
| |
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Treatment protocol | Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared on susceptible plants |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
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Sample description | 6H post inoculation |
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Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml |
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Protocols for BioSource 1 |
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Slide: Marco_2-16_Nd-1000-6H_Rep2_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
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Treatment protocol | Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared on susceptible plants |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
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Sample description | 6H post inoculation |
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Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml |
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Protocols for BioSource 1 |
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Slide: Marco_2-17_Nd-1000-12H_Rep1_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
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Treatment protocol | Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared on susceptible plants. |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
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Sample description | 12H post inoculation. |
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Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. |
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Protocols for BioSource 1 |
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Slide: Marco_2-18_Nd-1000-12H_Rep2_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
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Treatment protocol | Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared on susceptible plants. |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
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Sample description | 12H post inoculation. |
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Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. |
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Protocols for BioSource 1 |
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Slide: Marco_2-19_Nd-1000-24H_Rep1_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
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Treatment protocol | Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared on susceptible plants |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
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Sample description | 24H post inoculation. |
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Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. |
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Protocols for BioSource 1 |
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Slide: Marco_2-20_Nd-1000-24H_Rep2_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
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Treatment protocol | Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared on susceptible plants |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
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Sample description | 24H post inoculation. |
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Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. |
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Protocols for BioSource 1 |
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Slide: Marco_2-21_Nd-1000-D1_Rep1_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
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Treatment protocol | Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared on susceptible plants |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
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Sample description | 5 days post inoculation. |
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Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. |
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Protocols for BioSource 1 |
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Slide: Marco_2-22_Nd-1000-D1_Rep2_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
| |
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Treatment protocol | Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared on susceptible plants |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
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Sample description | 5 days post inoculation. |
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Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. |
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Protocols for BioSource 1 |
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Slide: Marco_2-23_Nd-1000-D3_Rep1_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
| |
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Treatment protocol | Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared on susceptible plants |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
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Sample description | 8 days post inoculation. |
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Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. |
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Protocols for BioSource 1 |
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Slide: Marco_2-24_Nd-1000-D3_Rep2_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
| |
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Treatment protocol | Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared on susceptible plants |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
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Sample description | 8 days post inoculation. |
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Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. |
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Protocols for BioSource 1 |
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Slide: Marco_2-25_Nd-DeltaPopP2-6H_Rep1_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
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Treatment protocol | Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
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Sample description | 6H post inoculation |
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Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. |
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Protocols for BioSource 1 |
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Slide: Marco_2-26_Nd-DeltaPopP2-6H_Rep2_ATH1 | | |
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Tissue:
| Rosette |
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in vivo Treatment:
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Treatment protocol | 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared |
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Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
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Other Information:
| |
---|
Sample description | 6H post inoculation |
---|
Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Marco_2-27_Nd-DeltaPopP2-12H_Rep1_ATH1 | | |
|
|
|
Tissue:
| Rosette |
---|
in vivo Treatment:
| |
---|
Treatment protocol | 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared |
---|
Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
---|
Other Information:
| |
---|
Sample description | 12H post inoculation |
---|
Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Marco_2-28_Nd-DeltaPopP2-12H_Rep2_ATH1 | | |
|
|
|
Tissue:
| Rosette |
---|
in vivo Treatment:
| |
---|
Treatment protocol | 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared |
---|
Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
---|
Other Information:
| |
---|
Sample description | 12H post inoculation |
---|
Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Marco_2-29_Nd-DeltaPopP2-24H_Rep1_ATH1 | | |
|
|
|
Tissue:
| Rosette |
---|
in vivo Treatment:
| |
---|
Treatment protocol | 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared |
---|
Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
---|
Other Information:
| |
---|
Sample description | 24H post inoculation |
---|
Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Marco_2-30_Nd-DeltaPopP2-24H_Rep2_ATH1 | | |
|
|
|
Tissue:
| Rosette |
---|
in vivo Treatment:
| |
---|
Treatment protocol | 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared |
---|
Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
---|
Other Information:
| |
---|
Sample description | 24H post inoculation |
---|
Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Marco_2-31_Nd-DeltaPopP2-D1_Rep1_ATH1 | | |
|
|
|
Tissue:
| Rosette |
---|
in vivo Treatment:
| |
---|
Treatment protocol | 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared |
---|
Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
---|
Other Information:
| |
---|
Sample description | 5 days post inoculation. 25% of wilted leaves: disease index 1, D1 |
---|
Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Marco_2-32_Nd-DeltaPopP2-D1_Rep2_ATH1 | | |
|
|
|
Tissue:
| Rosette |
---|
in vivo Treatment:
| |
---|
Treatment protocol | 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared |
---|
Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
---|
Other Information:
| |
---|
Sample description | 5 days post inoculation. 25% of wilted leaves: disease index 1, D1 |
---|
Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Marco_2-33_Nd-DeltaPopP2-D3_Rep1_ATH1 | | |
|
|
|
Tissue:
| Rosette |
---|
in vivo Treatment:
| |
---|
Treatment protocol | 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared |
---|
Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
---|
Other Information:
| |
---|
Sample description | 8 days post inoculation. 75% of wilted leaves: disease index 3, D3 |
---|
Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Marco_2-34_Nd-DeltaPopP2-D3_Rep2_ATH1 | | |
|
|
|
Tissue:
| Rosette |
---|
in vivo Treatment:
| |
---|
Treatment protocol | 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared |
---|
Separation Technique:
| NucleoSpin RNA II kit (Macherey-Nagel, GmbH&Co.KG, Düren, Germany) |
---|
Other Information:
| |
---|
Sample description | 8 days post inoculation. 75% of wilted leaves: disease index 3, D3 |
---|
Start time | When the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml |
---|
|
Protocols for BioSource 1 |
|
|
|
Problems? Comments? Suggestions? Contact the Affymetrix Team