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Experiment: Calcium and the clock
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-529
We are aiming to analyze calcium effect on the circadian clock. Untrained plants lacking a calcium rhythm and artificially restored rhythm via ectopic calcium pulse. Plants were grown 10days in continuous light and then subjected to calcium/water treatment. Ramping from the subjective dawn: 0.5, 1, 5, 10, 20, 50, 60 mM CaCl2 for an hour. Every point (sampling every 4h) made in duplicate, with a water treated control. WT col used.
About the ExperimenterName: | Dr Sylvain Aubry |
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Head of Lab Name: | Julian Hibberd |
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Lab:
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Address: | Department of Plant Sciences, University of Cambridge Downing street, Cambridge, Cambridgeshire, CB2 3EA, UNITED KINGDOM
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Postcode:
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Country:
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| Telephone Number:
| 0122330220 |
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are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| compound_based_treatment |
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Number of Slides: | 28 |
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| Experimental Parameters:
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parameter | compound_based_treatment |
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Quality Control Measures Taken:
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Aubry: Calcium and the clock
Slide: Aubry_1-1_Water_0h_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
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Additional Organism Information:
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Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
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Other Information:
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Timecourse start procedure | subjective dawn |
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Protocols for BioSource 1 |
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Slide: Aubry_1-2_Water_0h_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
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Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
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Other Information:
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Timecourse start procedure | subjective dawn |
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Protocols for BioSource 1 |
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Slide: Aubry_1-3_Water_4h_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
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Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
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Other Information:
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Timecourse start procedure | subjective dawn |
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Protocols for BioSource 1 |
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Slide: Aubry_1-4_Water_4h_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
---|
Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
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Other Information:
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Timecourse start procedure | subjective dawn |
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Protocols for BioSource 1 |
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Slide: Aubry_1-5_Water_8h_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
---|
Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
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Other Information:
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Timecourse start procedure | subjective dawn |
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Protocols for BioSource 1 |
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Slide: Aubry_1-6_Water_8h_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
---|
Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
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Other Information:
| |
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Timecourse start procedure | subjective dawn |
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|
Protocols for BioSource 1 |
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Slide: Aubry_1-7_Water_12h_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
---|
Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
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Other Information:
| |
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Timecourse start procedure | subjective dawn |
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|
Protocols for BioSource 1 |
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Slide: Aubry_1-8_Water_12h_Rep2_ATH1 | | |
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Tissue:
| whole plant |
---|
in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
---|
Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
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Other Information:
| |
---|
Timecourse start procedure | subjective dawn |
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|
Protocols for BioSource 1 |
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Slide: Aubry_1-9_Water_16h_Rep1_ATH1 | | |
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Tissue:
| whole plant |
---|
in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
---|
Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
---|
Other Information:
| |
---|
Timecourse start procedure | subjective dawn |
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|
Protocols for BioSource 1 |
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Slide: Aubry_1-10_Water_16h_Rep2_ATH1 | | |
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Tissue:
| whole plant |
---|
in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
---|
Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
---|
Other Information:
| |
---|
Timecourse start procedure | subjective dawn |
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|
Protocols for BioSource 1 |
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Slide: Aubry_1-11_Water_20h_Rep1_ATH1 | | |
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Tissue:
| whole plant |
---|
in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
---|
Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
---|
Other Information:
| |
---|
Timecourse start procedure | subjective dawn |
---|
|
Protocols for BioSource 1 |
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Slide: Aubry_1-12_Water_20h_Rep2_ATH1 | | |
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|
Tissue:
| whole plant |
---|
in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
---|
Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
---|
Other Information:
| |
---|
Timecourse start procedure | subjective dawn |
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|
Protocols for BioSource 1 |
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Slide: Aubry_1-13_Water_24h_Rep1_ATH1 | | |
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Tissue:
| whole plant |
---|
in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
---|
Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
---|
Other Information:
| |
---|
Timecourse start procedure | subjective dawn |
---|
|
Protocols for BioSource 1 |
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Slide: Aubry_1-14_Water_24h_Rep2_ATH1 | | |
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|
Tissue:
| whole plant |
---|
in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
---|
Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
---|
Other Information:
| |
---|
Timecourse start procedure | subjective dawn |
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|
Protocols for BioSource 1 |
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Slide: Aubry_1-15_Calcium_0h_Rep1_ATH1 | | |
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Tissue:
| whole plant |
---|
in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
---|
Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
---|
Other Information:
| |
---|
Timecourse start procedure | subjective dawn |
---|
|
Protocols for BioSource 1 |
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Slide: Aubry_1-16_Calcium_0h_Rep2_ATH1 | | |
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|
Tissue:
| whole plant |
---|
in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
---|
Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
---|
Other Information:
| |
---|
Timecourse start procedure | subjective dawn |
---|
|
Protocols for BioSource 1 |
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Slide: Aubry_1-17_Calcium_4h_Rep1_ATH1 | | |
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|
Tissue:
| whole plant |
---|
in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
---|
Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
---|
Other Information:
| |
---|
Timecourse start procedure | subjective dawn |
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|
Protocols for BioSource 1 |
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Slide: Aubry_1-18_Calcium_4h_Rep2_ATH1 | | |
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|
Tissue:
| whole plant |
---|
in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
---|
Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
---|
Other Information:
| |
---|
Timecourse start procedure | subjective dawn |
---|
|
Protocols for BioSource 1 |
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Slide: Aubry_1-19_Calcium_8h_Rep1_ATH1 | | |
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|
Tissue:
| whole plant |
---|
in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
---|
Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
---|
Other Information:
| |
---|
Timecourse start procedure | subjective dawn |
---|
|
Protocols for BioSource 1 |
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|
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Slide: Aubry_1-20_Calcium_8h_Rep2_ATH1 | | |
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|
Tissue:
| whole plant |
---|
in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
---|
Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
---|
Other Information:
| |
---|
Timecourse start procedure | subjective dawn |
---|
|
Protocols for BioSource 1 |
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Slide: Aubry_1-21_Calcium_12h_Rep1_ATH1 | | |
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|
Tissue:
| whole plant |
---|
in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
---|
Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
---|
Other Information:
| |
---|
Timecourse start procedure | subjective dawn |
---|
|
Protocols for BioSource 1 |
|
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Slide: Aubry_1-22_Calcium_12h_Rep2_ATH1 | | |
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|
Tissue:
| whole plant |
---|
in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
---|
Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
---|
Other Information:
| |
---|
Timecourse start procedure | subjective dawn |
---|
|
Protocols for BioSource 1 |
|
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Slide: Aubry_1-23_Calcium_16h_Rep1_ATH1 | | |
|
|
|
Tissue:
| whole plant |
---|
in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
---|
Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
---|
Other Information:
| |
---|
Timecourse start procedure | subjective dawn |
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|
Protocols for BioSource 1 |
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Slide: Aubry_1-24_Calcium_16h_Rep2_ATH1 | | |
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Tissue:
| whole plant |
---|
in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
---|
Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
---|
Other Information:
| |
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Timecourse start procedure | subjective dawn |
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|
Protocols for BioSource 1 |
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Slide: Aubry_1-25_Calcium_20h_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
---|
Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
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Other Information:
| |
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Timecourse start procedure | subjective dawn |
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|
Protocols for BioSource 1 |
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Slide: Aubry_1-26_Calcium_20h_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
---|
Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
---|
Other Information:
| |
---|
Timecourse start procedure | subjective dawn |
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|
Protocols for BioSource 1 |
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|
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Slide: Aubry_1-27_Calcium_24h_Rep1_ATH1 | | |
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Tissue:
| whole plant |
---|
in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
---|
Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
---|
Other Information:
| |
---|
Timecourse start procedure | subjective dawn |
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|
Protocols for BioSource 1 |
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Slide: Aubry_1-28_Calcium_24h_Rep2_ATH1 | | |
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Tissue:
| whole plant |
---|
in vivo Treatment:
| From the subjective dawn,
a calcium ramp was applied,
every hour from 0.5, 1, 5, 10, 20, 50, 60 mM then in water the following hours. |
---|
Additional Organism Information:
| |
---|
Sample Description | bulk of 4-6 seedlings, at 2 leaf stage |
---|
Other Information:
| |
---|
Timecourse start procedure | subjective dawn |
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|
Protocols for BioSource 1 |
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