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Experiment: To identify changes in gene expression during silique senescence in Arabidopsis thaliana
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-82
The aim of the experiment is to compare and contrast the profiles of gene expression during silique senescence and to identify the molecular events that regulate the timing of the process. Silique occurs over a highly predictable time frame and as a consequence it may be easier to identify the molecular events that are critical for the process to take place than in systems such as leaf senescence. The material will be collected from 20 Arabidopsis thaliana ecotype Columbia plants (NASC order number N1093) grown under controlled conditions specified under NASC protocol: Baseline sample preparation. Two pods will be collected from each plant and pooled from 20 plants to reduce sampling variability. It is intended that 3 replicates of the experiment will be submitted. The RNA will be extracted from mature green silique tissues of 10 days after anthesis and senescing pod tissues of 20 days after anthesis using lithium chloride extraction protocol and cleaned with Qiagen RNAeasy column.
About the ExperimenterName: | Mr Thomas Yang |
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Head of Lab Name: | Professor Jerry Roberts |
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Lab:
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Institute:
| University of Nottingham |
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Address: | Division of Plant Sciences University of Nottingham Sutton Bonington Campus Loughborough Leicestershire
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Postcode:
| LE12 5RD |
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Country:
| UK |
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| Telephone Number:
| 01159516378 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
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Number of Slides: | 6 |
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| Experimental Parameters:
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parameter | age |
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Parameter | developmental_stage |
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Quality Control Measures Taken:
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no-plants-pooled | 20 |
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Yang_genome
Slide: Yang_1-1_young-pod_Rep1_ATH1 | | |
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Tissue:
| mature and senescing siliques |
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Diseased:
| Normal |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Yang_1-2_old-pod_Rep1_ATH1 | | |
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Tissue:
| mature and senescing siliques |
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Diseased:
| Normal |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Yang_1-3_young-pod_Rep2_ATH1 | | |
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Tissue:
| mature and senescing siliques |
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Diseased:
| Normal |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Yang_1-4_old-pod_Rep2_ATH1 | | |
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Tissue:
| mature and senescing siliques |
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Diseased:
| Normal |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Yang_1-5_young-pod_Rep3_ATH1 | | |
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Tissue:
| mature and senescing siliques |
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Diseased:
| Normal |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Yang_1-6_old-pod_Rep3_ATH1 | | |
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Tissue:
| mature and senescing siliques |
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Diseased:
| Normal |
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Other Information:
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Protocols for BioSource 1 |
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Problems? Comments? Suggestions? Contact the Affymetrix Team