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Experiment: P G62 study
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-590
Analysis of root transcriptome of wild type (Col-0) and mutant (pen1-3) Arabidopsis plants after seed inoculation with the growth promoting rhizobacterium Pseudomonas sp. G62.
About the ExperimenterName: | Melanie Schwochow |
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Head of Lab Name: | Dr Joost van Dongen |
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Lab:
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Address: | Department 1: Molecular Physiology, Max-Planck-Institut fur Molekulare Pflanzenphysiologie Wissenschaftspark Golm, Am Mühlenberg1, Potsdam, Golm, Brandenburg, 14476, GERMANY
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Postcode:
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Country:
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| Telephone Number:
| 0331-5678345 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| genetic_modification_design, stimulus_or_stress_design |
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Number of Slides: | 12 |
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| Experimental Parameters:
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parameter | rhizobacterium treatment of seeds |
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Quality Control Measures Taken:
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Schwochow: P G62 study
Slide: Schwochow_590-1_Col-0_Control_Root_Rep1_ATH1 | | |
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Tissue:
| roots |
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Additional Organism Information:
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Sample Description | - age: 33days old
- phenotype: inoculated plants grow significantly higher
- roots: grow until the end of the plate |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Schwochow_590-2_Col-0_Control_Root_Rep2_ATH1 | | |
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Tissue:
| roots |
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Additional Organism Information:
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Sample Description | - age: 33days old
- phenotype: inoculated plants grow significantly higher
- roots: grow until the end of the plate |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Schwochow_590-3_Col-0_Control_Root_Rep3_ATH1 | | |
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Tissue:
| roots |
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Additional Organism Information:
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Sample Description | - age: 33days old
- phenotype: inoculated plants grow significantly higher
- roots: grow until the end of the plate |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Schwochow_590-4_Col-0_Bacteria_Root_Rep1_ATH1 | | |
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Tissue:
| roots |
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Additional Organism Information:
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Sample Description | - age: 33days old
- phenotype: inoculated plants grow significantly higher
- roots: grow until the end of the plate |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Schwochow_590-5_Col-0_Bacteria_Root_Rep2_ATH1 | | |
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Tissue:
| roots |
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in vivo Treatment:
| - measure OD (around 0,6)
- take 2ml ³ centrifuge for 1 minute at 12000rpm
- discard supernatant
- wash pellet with 10mM MgSO4 ³ centrifuge for 1minute at 12000rpm
- discard supernatant
- resolve the bacteria pellet in 2ml 10mM MgSO4
- discard tap water from the seeds
- bacteria solution to the seeds
- incubate for 3 hours
- shake slightly every 30 minutes |
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Additional Organism Information:
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Sample Description | - age: 33days old
- phenotype: inoculated plants grow significantly higher
- roots: grow until the end of the plate |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Schwochow_590-6_Col-0_Bacteria_Root_Rep3_ATH1 | | |
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Tissue:
| roots |
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in vivo Treatment:
| - measure OD (around 0,6)
- take 2ml ³ centrifuge for 1 minute at 12000rpm
- discard supernatant
- wash pellet with 10mM MgSO4 ³ centrifuge for 1minute at 12000rpm
- discard supernatant
- resolve the bacteria pellet in 2ml 10mM MgSO4
- discard tap water from the seeds
- bacteria solution to the seeds
- incubate for 3 hours
- shake slightly every 30 minutes |
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Additional Organism Information:
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Sample Description | - age: 33days old
- phenotype: inoculated plants grow significantly higher
- roots: grow until the end of the plate |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Schwochow_590-7_pen1-3_Control_Root_Rep1_ATH1 | | |
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Tissue:
| roots |
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Additional Organism Information:
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Sample Description | - age: 33days old
- phenotype: inoculated plants grow significantly higher
- roots: grow until the end of the plate |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Schwochow_590-8_pen1-3_Control_Root_Rep2_ATH1 | | |
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Tissue:
| roots |
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Additional Organism Information:
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Sample Description | - age: 33days old
- phenotype: inoculated plants grow significantly higher
- roots: grow until the end of the plate |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Schwochow_590-9_pen1-3_Control_Root_Rep3_ATH1 | | |
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Tissue:
| roots |
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Additional Organism Information:
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Sample Description | - age: 33days old
- phenotype: inoculated plants grow significantly higher
- roots: grow until the end of the plate |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Schwochow_590-10_pen1-3_Bacteria_Root_Rep1_ATH1 | | |
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Tissue:
| roots |
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in vivo Treatment:
| - measure OD (around 0,6)
- take 2ml ³ centrifuge for 1 minute at 12000rpm
- discard supernatant
- wash pellet with 10mM MgSO4 ³ centrifuge for 1minute at 12000rpm
- discard supernatant
- resolve the bacteria pellet in 2ml 10mM MgSO4
- discard tap water from the seeds
- bacteria solution to the seeds
- incubate for 3 hours
- shake slightly every 30 minutes |
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Additional Organism Information:
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Sample Description | - age: 33days old
- phenotype: inoculated plants grow significantly higher
- roots: grow until the end of the plate |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Schwochow_590-11_pen1-3_Bacteria_Root_Rep1_ATH1 | | |
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Tissue:
| roots |
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in vivo Treatment:
| - measure OD (around 0,6)
- take 2ml ³ centrifuge for 1 minute at 12000rpm
- discard supernatant
- wash pellet with 10mM MgSO4 ³ centrifuge for 1minute at 12000rpm
- discard supernatant
- resolve the bacteria pellet in 2ml 10mM MgSO4
- discard tap water from the seeds
- bacteria solution to the seeds
- incubate for 3 hours
- shake slightly every 30 minutes |
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Additional Organism Information:
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Sample Description | - age: 33days old
- phenotype: inoculated plants grow significantly higher
- roots: grow until the end of the plate |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Schwochow_590-12_pen1-3_Bacteria_Root_Rep2_ATH1 | | |
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Tissue:
| roots |
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in vivo Treatment:
| - measure OD (around 0,6)
- take 2ml ³ centrifuge for 1 minute at 12000rpm
- discard supernatant
- wash pellet with 10mM MgSO4 ³ centrifuge for 1minute at 12000rpm
- discard supernatant
- resolve the bacteria pellet in 2ml 10mM MgSO4
- discard tap water from the seeds
- bacteria solution to the seeds
- incubate for 3 hours
- shake slightly every 30 minutes |
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Additional Organism Information:
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Sample Description | - age: 33days old
- phenotype: inoculated plants grow significantly higher
- roots: grow until the end of the plate |
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Other Information:
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Protocols for BioSource 1 |
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