 | NASCArrays | ,
,
,
,
,
,
,
,
,
,
|
Experiment: Arabidopsis thaliana/Phytophthora parasitica compatible interaction transcriptome
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-468
Oomycetes from the genus Phytophthora are fungus-like plant pathogens that are devastating for agriculture and natural ecosystems. Due to particular physiological characteristics, no treatments against diseases caused by oomycetes are presently available. To develop such treatments, it appears essential to dissect the molecular mechanisms that determine the interaction between Phytophthora species and host plants. The present project is focused on the molecular mechanisms that underlie the compatible plant-oomycete interaction and plant disease.The laboratory developed a novel interaction system involving the model plant, Arabidopsis thaliana and Phytophthora parasitica, a soil-borne pathogen infecting a wide host range, thus representing the majority of Phytophthora species. A characteristic feature of the compatible Arabidopsis/P. parasitica interaction is an extended biotrophic phase, before infection becomes necrotrophic. Because the initial biotrophic phase is extremely short on natural (e.g. solanaceous) hosts, the Arabidopsis system provides the opportunity to analyze, for both interaction partners, the molecular events that determine the initiation of infection and the switch to necrotrophy.The present project aims at analyzing the compatible interaction between A. thaliana roots and P. parasitica. The Affymetrix A. thaliana full genome chip will be used to characterize modulations of the transcriptome occurring over a period of 24h from the onset of plant root infection to the beginning of necrotrophy. Parallel to this study, a custom designed P. parasitica biochip will enable analyzing of P. parasitica gene expression during the same stages.
About the ExperimenterName: | Agnes Attard |
---|
Head of Lab Name: | Harald Keller |
---|
Lab:
| |
---|
Address: | 400, route des Chappes BP 167
|
---|
Postcode:
| F-06903 Sophia-antipolis |
---|
Country:
| France |
---|
| Telephone Number:
| 33492386594 |
---|
Fax Number:
| 33492386587 |
---|
All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
|
| About this ExperimentExperiment Type:
| pathogenicity_design |
---|
Number of Slides: | 10 |
---|
| Experimental Parameters:
| |
---|
parameter | infect |
---|
Quality Control Measures Taken:
|
|
---|
no-plants-pooled | |
---|
References:
| |
---|
| Other Information:
| |
---|
|
Slides in this Experiment
Hybridisation Set: Attard: Arabidopsis thaliana/Phytophthora parasitica compatible interaction transcriptome_genome
Slide: Attard_1-1_At-Control_Rep1_ATH1 | | |
|
|
|
Tissue:
| roots |
---|
Additional Organism Information:
| |
---|
Sample Description | mock inoculated roots |
---|
Other Information:
| |
---|
Timecourse start procedure | Start time was time when plants were inoculated |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Attard_1-2_At-Control_Rep2_ATH1 | | |
|
|
|
Tissue:
| roots |
---|
Additional Organism Information:
| |
---|
Sample Description | mock inoculated roots |
---|
Other Information:
| |
---|
Timecourse start procedure | Start time was time when plants were inoculated |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Attard_1-3_At-Pp-2.5-hai_Rep1_ATH1 | | |
|
|
|
Tissue:
| roots |
---|
Additional Organism Information:
| |
---|
Sample Description | A. thaliana roots were inoculated with Phytophthora parasitica 310 strain (10E6 zoospores per Petri dish |
---|
Other Information:
| |
---|
Timecourse start procedure | Start time was time when plants were inoculated |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Attard_1-4_At-Pp-2.5-hai_Rep2_ATH1 | | |
|
|
|
Tissue:
| roots |
---|
Additional Organism Information:
| |
---|
Sample Description | A. thaliana roots were inoculated with Phytophthora parasitica 310 strain (10E6 zoospores per Petri dish |
---|
Other Information:
| |
---|
Timecourse start procedure | Start time was time when plants were inoculated |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Attard_1-5_At-Pp-6-hai_Rep1_ATH1 | | |
|
|
|
Tissue:
| roots |
---|
Additional Organism Information:
| |
---|
Sample Description | A. thaliana roots were inoculated with Phytophthora parasitica 310 strain (10E6 zoospores per Petri dish |
---|
Other Information:
| |
---|
Timecourse start procedure | Start time was time when plants were inoculated |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Attard_1-6_At-Pp-6-hai_Rep2_ATH1 | | |
|
|
|
Tissue:
| roots |
---|
Additional Organism Information:
| |
---|
Sample Description | A. thaliana roots were inoculated with Phytophthora parasitica 310 strain (10E6 zoospores per Petri dish |
---|
Other Information:
| |
---|
Timecourse start procedure | Start time was time when plants were inoculated |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Attard_1-7_At-Pp-10.5-hai_Rep1_ATH1 | | |
|
|
|
Tissue:
| roots |
---|
Additional Organism Information:
| |
---|
Sample Description | A. thaliana roots were inoculated with Phytophthora parasitica 310 strain (10E6 zoospores per Petri dish |
---|
Other Information:
| |
---|
Timecourse start procedure | Start time was time when plants were inoculated |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Attard_1-8_At-Pp-10.5-hai_Rep2_ATH1 | | |
|
|
|
Tissue:
| roots |
---|
Additional Organism Information:
| |
---|
Sample Description | A. thaliana roots were inoculated with Phytophthora parasitica 310 strain (10E6 zoospores per Petri dish |
---|
Other Information:
| |
---|
Timecourse start procedure | Start time was time when plants were inoculated |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Attard_1-9_At-Pp-30-hai_Rep1_ATH1 | | |
|
|
|
Tissue:
| roots |
---|
Additional Organism Information:
| |
---|
Sample Description | A. thaliana roots were inoculated with Phytophthora parasitica 310 strain (10E6 zoospores per Petri dish |
---|
Other Information:
| |
---|
Timecourse start procedure | Start time was time when plants were inoculated |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Attard_1-10_At-Pp-30-hai_Rep2_ATH1 | | |
|
|
|
Tissue:
| roots |
---|
Additional Organism Information:
| |
---|
Sample Description | A. thaliana roots were inoculated with Phytophthora parasitica 310 strain (10E6 zoospores per Petri dish |
---|
Other Information:
| |
---|
Timecourse start procedure | Start time was time when plants were inoculated |
---|
|
Protocols for BioSource 1 |
|
|
|
Problems? Comments? Suggestions? Contact the Affymetrix Team