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Experiment: The effects of infection with wild-type or mutant cucumber mosaic virus on the Arabidopsis transcriptome
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-552
The cucumber mosaic virus (CMV) 2b counter-defense protein disrupts plant antiviral mechanisms mediated by RNA silencing and salicylic acid (SA). Using NASC ATH-1 microarrays to investigate defensive gene expression in 2b-transgenic Arabidopsis thaliana plants we found that, surprisingly, 2b inhibited expression of few SA-regulated genes and in some instances enhanced the effect of SA on certain genes. Strikingly, the 2b protein inhibited changes in the expression of 90% of genes regulated by jasmonic acid (NASCARRAYS-415: Lewsey et al. Molec. Plant-Microbe Interact. vol. 23, in press). We will extend this work to understand the effects of the 2b protein on plant gene expression during an authentic viral infection. By comparing the effects on the transcriptome of infection by wild-type CMV and the 2b gene deletion mutant, CMV∆2b, we will reveal not only the influence of the 2b protein but also the effects of its interactions with other viral gene products (and the process of infection itself), on the host transcriptome.
About the ExperimenterName: | Dr Zhiyou Du |
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Head of Lab Name: | John Carr |
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Lab:
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Address: | Department of Plant Sciences, University of Cambridge Downing street, Cambridge, Cambridgeshire, CB2 3EA, UNITED KINGDOM
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Postcode:
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Country:
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| Telephone Number:
| 01223330215 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| pathogenicity_design |
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Number of Slides: | 9 |
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| Experimental Parameters:
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parameter | infect |
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Quality Control Measures Taken:
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References:
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| Other Information:
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other factors | virus challenge |
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Slides in this Experiment
Hybridisation Set: Du: The effects of infection with wild-type or mutant cucumber mosaic virus on the Arabidopsis transcriptome
Slide: Du_552-1_Mock-At_Rep1_ATH1 | | |
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Tissue:
| Aerial tissues |
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in vivo Treatment:
| Approximately 24 plants were inoculated with distilled water when plants were 3 weeks old (growth stage 1.05 to 1.07). Aerial tissues of total 16 plants were harvested 14 days post inoculation ((growth stage 3.50 to 3.70) pooled to minimise variation. The pooled tissue was then used for total RNA extraction. |
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Additional Organism Information:
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Sample Description | |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Du_552-2_CMV-WT_Rep1_ATH1 | | |
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Tissue:
| Aerial tissues |
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in vivo Treatment:
| Approximately 24 plants were inoculated with wildtype Fny-CMV (CMV-WT) when plants were 3 weeks old (growth stage 1.05 to 1.07). Aerial tissues of total 16 plants were harvested 14 days post inoculation ((growth stage 3.50 to 3.70) pooled to minimise variation. The pooled tissue was then used for total RNA extraction. |
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Additional Organism Information:
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Sample Description | |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Du_552-3_CMV-A2b_mutant_Rep1_ATH1 | | |
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Tissue:
| Aerial tissues |
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in vivo Treatment:
| Approximately 24 plants were inoculated with purified Fny-CMV∆2b (CMV-∆2b) virion of 100 ng/µl when plants were 3 weeks old (growth stage 1.05 to 1.07). Aerial tissues of total 16 plants were harvested 14 days post inoculation ((growth stage 3.50 to 3.70) pooled to minimise variation. The pooled tissue was then used for total RNA extraction. |
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Additional Organism Information:
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Sample Description | |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Du_552-4_Mock-At_Rep2_ATH1 | | |
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Tissue:
| Aerial tissues |
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in vivo Treatment:
| Approximately 24 plants were inoculated with distilled water when plants were 3 weeks old (growth stage 1.05 to 1.07). Aerial tissues of total 16 plants were harvested 14 days post inoculation ((growth stage 3.50 to 3.70) pooled to minimise variation. The pooled tissue was then used for total RNA extraction. |
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Additional Organism Information:
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Sample Description | |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Du_552-5_CMV-WT_Rep2_ATH1 | | |
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Tissue:
| Aerial tissues |
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in vivo Treatment:
| Approximately 24 plants were inoculated with wildtype Fny-CMV (CMV-WT) when plants were 3 weeks old (growth stage 1.05 to 1.07). Aerial tissues of total 16 plants were harvested 14 days post inoculation ((growth stage 3.50 to 3.70) pooled to minimise variation. The pooled tissue was then used for total RNA extraction. |
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Additional Organism Information:
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Sample Description | |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Du_552-6_CMV-A2b_mutant_Rep2_ATH1 | | |
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Tissue:
| Aerial tissues |
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in vivo Treatment:
| Approximately 24 plants were inoculated with purified Fny-CMVΔ2b (CMV-Δ2b) virion of 100 ng/µl when plants were 3 weeks old (growth stage 1.05 to 1.07). Aerial tissues of total 16 plants were harvested 14 days post inoculation ((growth stage 3.50 to 3.70) pooled to minimise variation. The pooled tissue was then used for total RNA extraction. |
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Additional Organism Information:
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Sample Description | |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Du_552-7_Mock-At_Rep3_ATH1 | | |
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Tissue:
| Aerial tissues |
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in vivo Treatment:
| Approximately 24 plants were inoculated with distilled water when plants were 3 weeks old (growth stage 1.05 to 1.07). Aerial tissues of total 16 plants were harvested 14 days post inoculation ((growth stage 3.50 to 3.70) pooled to minimise variation. The pooled tissue was then used for total RNA extraction. |
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Additional Organism Information:
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Sample Description | |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Du_552-8_CMV-WT_Rep3_ATH1 | | |
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Tissue:
| Aerial tissues |
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in vivo Treatment:
| Approximately 24 plants were inoculated with purified wildtype Fny-CMV (CMV-WT) virion of 100 ng/µl when plants were 3 weeks old (growth stage 1.05 to 1.07). Aerial tissues of total 16 plants were harvested 14 days post inoculation ((growth stage 3.50 to 3.70) pooled to minimise variation. The pooled tissue was then used for total RNA extraction. |
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Additional Organism Information:
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Sample Description | |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Du_552-9_CMV-A2b_mutant_Rep3_ATH1 | | |
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Tissue:
| Aerial tissues |
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in vivo Treatment:
| Approximately 24 plants were inoculated with purified Fny-CMVΔ2b (CMV-Δ2b) virion of 100 ng/µl when plants were 3 weeks old (growth stage 1.05 to 1.07). Aerial tissues of total 11 plants were harvested 14 days post inoculation ((growth stage 3.50 to 3.70) pooled to minimise variation. The pooled tissue was then used for total RNA extraction. |
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Additional Organism Information:
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Sample Description | |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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