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Experiment: Biological function of Pip3
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-483
Ralstonia solanacearum is the causal agent of bacterial wilt. Arabidopsis thaliana monogenic resistance to the strain GMI1000 is conferred by the RRS1-R gene, present in Nd-1 resistant ecotype and absent in the susceptible ecotype Col-0. The corresponding protein is recognized by the avirulence protein PopP2 of R. solanacearum. In order to identify the plant proteins involved in the PopP2/RRS1-R perception complex, the screening of a root cDNA library in yeast was performed using PopP2 as bait. One interactor found was called Pip3, and we would like understanding better its biological function. Thereby we choose to compare two RNAi lines with their wild type Nd-1 background, and one KO line with its wilt type Col-0 background. Also, two overexpressor lines will be compared with their wilt type Nd-1 background and two with their Col-0 background.
About the ExperimenterName: | Dr Yves Marco |
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Head of Lab Name: | Dr Pascal Gamas |
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Lab:
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Address: | LIPM UMR CNRS/INRA 2594/441 chemin de Borderouge BP 52627 Castanet-Tolosan
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Postcode:
| 31326 |
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Country:
| France |
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| Telephone Number:
| 33561285509 |
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Fax Number:
| 33261285061 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| genetic_modification_design |
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Number of Slides: | 27 |
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| Experimental Parameters:
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parameter | gene_knock_in |
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parameter | gene_knock_out |
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Quality Control Measures Taken:
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no-plants-pooled | |
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References:
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Slides in this Experiment
Hybridisation Set: Marco: Biological function of Pip3_genome
Slide: Marco_3-27_KO-Pip3-3_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber and RNA was extracted from whole plantlets |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Marco_3-26_pGli-69-24-3_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber and RNA was extracted from whole plantlets.
Genetic background is Nd-1. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Marco_3-25_pGli-69-31-3_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber and RNA was extracted from whole plantlets.
Genetic background is Nd-1. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Marco_3-24_Pip3-3HA6H-5-3_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber and RNA was extracted from whole plantlets. The background of this line is Nd-1. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Marco_3-23_Pip3-3HA6H-1-3_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber and RNA was extracted from whole plantlets. The background of this line is Nd-1. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Marco_3-22_Pip3-3HA-9-3_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber. The RNA was extracted from these whole plantlets. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Marco_3-21_Pip3-3HA-1-3_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber. The RNA was extracted from these whole plantlets. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Marco_3-20_Nd-1-3_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber. The RNA was extracted from these whole plantlets. |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Marco_3-19_Col-0-3_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber. The RNA was extracted from these whole plantlets. |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Marco_3-18_KO-Pip3-2_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber and RNA was extracted from whole plantlets |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Marco_3-17_pGli-69-24-2_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber. The RNA was extracted from these whole plantlets. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Marco_3-16_pGli-69-31-2_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber. The RNA was extracted from these whole plantlets. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Marco_3-15_Pip3-3HA6H-5-2_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber and RNA was extracted from whole plantlets. The background of this line is Nd-1. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Marco_3-14_Pip3-3HA6H-1-2_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber and RNA was extracted from whole plantlets. The background of this line is Nd-1. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Marco_3-13_Pip3-3HA-9-2_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber and RNA was extracted from whole plantlets. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Marco_3-12_Pip3-3HA-1-2_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber. The RNA was extracted from these whole plantlets. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Marco_3-11_Nd-1-2_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber. The RNA was extracted from these whole plantlets. |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Marco_3-10_Col-0-2_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber. The RNA was extracted from these whole plantlets. |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Marco_3-9_KO-Pip3_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber and RNA was extracted from whole plantlets |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Marco_3-8_pGli-69-24_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber. The RNA was extracted from these whole plantlets. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Marco_3-7_pGli-69-31_Rep1_ATH1_2 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber. The RNA was extracted from these whole plantlets. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Marco_3-6_Pip3-3HA6H-5_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber and RNA was extracted from whole plantlets. The background of this line is Nd-1. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Marco_3-5_Pip3-3HA6H-1_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber and RNA was extracted from whole plantlets. The background of this line is Nd-1. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Marco_3-4_Pip3-3HA-9_Rep1_ATH1_2 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber and RNA was extracted from whole plantlets. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Marco_3-3_Pip3-3HA-1_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber. The RNA was extracted from these whole plantlets. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Marco_3-2_Nd-1_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber and RNA was extracted from whole plantlets |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Marco_3-1_Col-0_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Seeds were sown on MS medium (Murashige and Skoog 1962). Plantlets grown for 8 days at 20°C in a growth chamber. The RNA was extracted from these whole plantlets. |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Problems? Comments? Suggestions? Contact the Affymetrix Team