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Experiment: Identification of Genes Differentially Expressed in fry 2-1 Mutants
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-611
Low temperature, drought, and high salinity induce the expression of many plant genes. Recessive mutations in FIERY2 (FRY2) result in super-induction of the DRE/CRT class of stress-responsive genes, suggesting that FRY2 negatively regulates stress and ABA activation of these genes. The aim of this experiment is to identify genes differentially expressed in fry 2-1 mutant and their comparison with C24 wild type Arabidopsis plants. For this purpose, both C24 and fry2-1 mutant seeds were surface sterilized by 70% ethanol and washed with distilled water for several times. Seeds were grown on 1/4 strength Murashigee and Skoog basal media containing 0.5 % sucrose, solidified with 0.8 % plant cell tissue grade agar. Approximately 200 seeds were sown on each petri dish and stratified for 4 days at 4°C. Then, they were transferred to a growth chamber providing 16 h of light and 8 h of darkness at 25°C.After 10 days of growth at these conditions, total RNA was extracted from 100mg of seedlings by using TRIzol (Invitrogen, Cat No. 15596-018) method according to the manufacturer's procedure. Then, total RNA samples were treated with DNase I (Qiagen, Cat. No. 79254) at 37°C for 1 hours and cleaned using Qiagen minicolumns.
About the ExperimenterName: | EMRE AKSOY |
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Head of Lab Name: | Dr. HISASHI KOIWA |
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Lab:
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Address: | Department of Horticultural Sciences Texas A & M University, College Station,Texas
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Postcode:
| 77843-2133 |
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Country:
| USA |
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| Telephone Number:
| 979-845-5282 |
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Fax Number:
| 979-845-0627 |
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are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| genetic_modification_design |
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Number of Slides: | 6 |
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| Experimental Parameters:
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parameter | gene_knock_out |
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Quality Control Measures Taken:
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References:
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Slides in this Experiment
Hybridisation Set: AKSOY: Identification of Genes Differentially Expressed in fry 2-1 Mutants
Slide: Aksoy_1-1_C24 _Rep1_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Whole plantlets were used as sample. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Aksoy_1-3_C24 _Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Whole plantlets were used as sample. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Aksoy_1-5_C24 _Rep3_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Whole plantlets were used as sample. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Aksoy_1-2_fry2-1 _Rep1_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Whole plantlets were used as sample. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Aksoy_1-4_fry2-1 _Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Whole plantlets were used as sample. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Aksoy_1-6_fry2-1 _Rep3_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Whole plantlets were used as sample. |
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Other Information:
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Protocols for BioSource 1 |
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