 | NASCArrays | ,
,
,
,
,
,
,
,
,
,
|
Experiment: Finlayson: Transcriptomics of Axillary Bud Outgrowth Regulation by Shade Signals in Arabidopsis.
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-561
Aims: To determine the changes in the Arabidopsis axillary bud transcriptome in response to changes in the red light (R) to far red light (FR) ratio (R:FR).Background: The branching habit of plants is a key determinant of overall plant form and function with great relevance to modern agriculture. Shade signals transduced by phytochromes are major regulators of axillary bud outgrowth, and in turn control branching in both natural and agricultural environments. To continue our investigations into the regulation of branching by R:FR, we have developed a system using supplemental FR LEDs to tightly control the outgrowth of Arabidopsis axillary buds. Depending on the position of the bud in the rosette, outgrowth is either repressed (uppermost bud) or rapidly promoted (bud in the axil of the third leaf down) by the transition from low to high R:FR.Treatment: Two separate experiments were conducted to evaluate the effects of R:FR on transcriptome changes in the uppermost rosette bud (bud n) and the axillary bud in the axil of the third leaf from the top (bud n-2). WT Col-60000 was used as the experimental material. Plants were grown individually in 25 by 50 mm tubes and watered and fertilized optimally. Plants were grown in a split growth chamber (providing uniform temperature and PPFD but allowing for differential R:FR) with 18 h photoperiods (185 Moles m-2 s-1 PPFD provided by T12 VHO CW fluorescent lamps) and 24oC/18oC day/night temperatures. One day after sowing the R:FR was reduced on both sides of the chamber from 3.5 to 0.08 using FR LEDs fixed in clear overhead arrays. Prior to anthesis the plants were matched and split into two treatment groups. In experiment 1 the FR source for one of the groups was switched off at 12:00 pm on the day of anthesis, causing the R:FR to increase to 3.5. Unelongated axillary buds in the axil of the uppermost leaves (approx. 2.5 mm long) were harvested for RNA preparation from both groups (low and transiently increased R:FR) 3 h after changing the R:FR. Each treatment was composed of three biological replicates, each containing buds from about 15-18 plants. Experiment 2 was conducted exactly the same as experiment 1, except the R:FR was altered 3 days after anthesis and the unelongated axillary buds in the axils of the third leaves down (approx. 1 mm long) were harvested for RNA preparation.
About the ExperimenterName: | Dr Scott Finlayson |
---|
Head of Lab Name: | Dr Scott Finlayson |
---|
Lab:
| |
---|
Address: | Department of Soil and Crop Sciences, College of Agriculture and Life Science, Texas A & M University Heep Ctr., 370 Olsen Blvd, College Station, Texas, 77843-2474, UNITED STATES
|
---|
Postcode:
| |
---|
Country:
| |
---|
| Telephone Number:
| 9798479287 |
---|
Fax Number:
| 9798450456 |
---|
All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
|
| About this ExperimentExperiment Type:
| stimulus_or_stress_design |
---|
Number of Slides: | 12 |
---|
| Experimental Parameters:
| |
---|
parameter | change_light |
---|
Quality Control Measures Taken:
| |
---|
References:
| |
---|
| Other Information:
| |
---|
|
Slides in this Experiment
Hybridisation Set: Finlayson: Transcriptomics of Axillary Bud Outgrowth Regulation by Shade Signals in Arabidopsis.
Slide: Finlayson_561-10_high-R:FR-bud-n-2_Rep1_ATH1 | | |
|
|
|
Tissue:
| Rosette axillary bud n-2 |
---|
Other Information:
| |
---|
treatment | Two separate experiments were conducted to evaluate the effects of R:FR on transcriptome changes in the uppermost rosette bud (bud n) and the axillary bud in the axil of the third leaf from the top (bud n-2). WT Col-0 (CS60000) was used as the experimental material. Plants were grown individually in 26 by 60 mm tubes and watered and fertilized optimally. Plants were grown in a split growth chamber (providing uniform temperature and PPFD but\
allowing for differential R:FR) with 18 h photoperiods (185 microMoles m-2 s-1 PPFD provided by T12 VHO CW fluorescent lamps) and 24oC/18oC day/night temperatures. One day after sowing the R:FR was reduced on both sides of the chamber from 3.5 to 0.08 using FR LEDs fixed in clear overhead arrays. Prior to anthesis the plants were matched and split into two treatment groups. In experiment 1 the FR source for one of the groups was switched off at 12:00 pm on the\
day of anthesis, causing the R:FR to increase to 3.5. Unelongated axillary buds in the axil of the uppermost leaves (approx. 2.5 mm long) were harvested for RNA preparation from both groups (low and transiently increased R:FR) 3 h after changing the R:FR. Each treatment was composed of three biological replicates, each containing buds from about 15-18 plants. Experiment 2 was conducted exactly the same as experiment 1, except the R:FR was altered 3 days after \
anthesis and the unelongated axillary buds in the axils of the third leaves down (approx. 1 mm long) were harvested for RNA preparation.This sample was derived from plants grown under low R:FR from 1 day after sowing until 3 days after anthesis. At 12:00 on the third day after anthesis, the plants were exposed to high R:FR for 3 h and rosette axillary bud n-2 was harvested. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Finlayson_561-9_low-R:FR-bud-n-2_Rep3_ATH1 | | |
|
|
|
Tissue:
| Rosette axillary bud n-2 |
---|
Other Information:
| |
---|
treatment | Two separate experiments were conducted to evaluate the effects of R:FR on transcriptome changes in the uppermost rosette bud (bud n) and the axillary bud in the axil of the third leaf from the top (bud n-2). WT Col-0 (CS60000) was used as the experimental material. Plants were grown individually in 26 by 60 mm tubes and watered and fertilized optimally. Plants were grown in a split growth chamber (providing uniform temperature and PPFD but\
allowing for differential R:FR) with 18 h photoperiods (185 microMoles m-2 s-1 PPFD provided by T12 VHO CW fluorescent lamps) and 24oC/18oC day/night temperatures. One day after sowing the R:FR was reduced on both sides of the chamber from 3.5 to 0.08 using FR LEDs fixed in clear overhead arrays. Prior to anthesis the plants were matched and split into two treatment groups. In experiment 1 the FR source for one of the groups was switched off at 12:00 pm on the\
day of anthesis, causing the R:FR to increase to 3.5. Unelongated axillary buds in the axil of the uppermost leaves (approx. 2.5 mm long) were harvested for RNA preparation from both groups (low and transiently increased R:FR) 3 h after changing the R:FR. Each treatment was composed of three biological replicates, each containing buds from about 15-18 plants. Experiment 2 was conducted exactly the same as experiment 1, except the R:FR was altered 3 days after \
anthesis and the unelongated axillary buds in the axils of the third leaves down (approx. 1 mm long) were harvested for RNA preparation.
This sample was derived from plants maintained under low R:FR from 1 day after sowing until harvest at 3 days after anthesis |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Finlayson_561-8_low-R:FR-bud-n-2_Rep2_ATH1 | | |
|
|
|
Tissue:
| Rosette axillary bud n-2 |
---|
Other Information:
| |
---|
treatment | Two separate experiments were conducted to evaluate the effects of R:FR on transcriptome changes in the uppermost rosette bud (bud n) and the axillary bud in the axil of the third leaf from the top (bud n-2). WT Col-0 (CS60000) was used as the experimental material. Plants were grown individually in 26 by 60 mm tubes and watered and fertilized optimally. Plants were grown in a split growth chamber (providing uniform temperature and PPFD but\
allowing for differential R:FR) with 18 h photoperiods (185 microMoles m-2 s-1 PPFD provided by T12 VHO CW fluorescent lamps) and 24oC/18oC day/night temperatures. One day after sowing the R:FR was reduced on both sides of the chamber from 3.5 to 0.08 using FR LEDs fixed in clear overhead arrays. Prior to anthesis the plants were matched and split into two treatment groups. In experiment 1 the FR source for one of the groups was switched off at 12:00 pm on the\
day of anthesis, causing the R:FR to increase to 3.5. Unelongated axillary buds in the axil of the uppermost leaves (approx. 2.5 mm long) were harvested for RNA preparation from both groups (low and transiently increased R:FR) 3 h after changing the R:FR. Each treatment was composed of three biological replicates, each containing buds from about 15-18 plants. Experiment 2 was conducted exactly the same as experiment 1, except the R:FR was altered 3 days after \
anthesis and the unelongated axillary buds in the axils of the third leaves down (approx. 1 mm long) were harvested for RNA preparation.
This sample was derived from plants maintained under low R:FR from 1 day after sowing until harvest at 3 days after anthesis |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Finlayson_561-7_low-R:FR-bud-n-2_Rep1_ATH1 | | |
|
|
|
Tissue:
| Rosette axillary bud n-2 |
---|
Other Information:
| |
---|
treatment | Two separate experiments were conducted to evaluate the effects of R:FR on transcriptome changes in the uppermost rosette bud (bud n) and the axillary bud in the axil of the third leaf from the top (bud n-2). WT Col-0 (CS60000) was used as the experimental material. Plants were grown individually in 26 by 60 mm tubes and watered and fertilized optimally. Plants were grown in a split growth chamber (providing uniform temperature and PPFD but\
allowing for differential R:FR) with 18 h photoperiods (185 microMoles m-2 s-1 PPFD provided by T12 VHO CW fluorescent lamps) and 24oC/18oC day/night temperatures. One day after sowing the R:FR was reduced on both sides of the chamber from 3.5 to 0.08 using FR LEDs fixed in clear overhead arrays. Prior to anthesis the plants were matched and split into two treatment groups. In experiment 1 the FR source for one of the groups was switched off at 12:00 pm on the\
day of anthesis, causing the R:FR to increase to 3.5. Unelongated axillary buds in the axil of the uppermost leaves (approx. 2.5 mm long) were harvested for RNA preparation from both groups (low and transiently increased R:FR) 3 h after changing the R:FR. Each treatment was composed of three biological replicates, each containing buds from about 15-18 plants. Experiment 2 was conducted exactly the same as experiment 1, except the R:FR was altered 3 days after \
anthesis and the unelongated axillary buds in the axils of the third leaves down (approx. 1 mm long) were harvested for RNA preparation.
This sample was derived from plants maintained under low R:FR from 1 day after sowing until harvest at 3 days after anthesis |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Finlayson_561-6_high-R:FR-bud-n_Rep3_ATH1 | | |
|
|
|
Tissue:
| Rosette axillary bud n |
---|
Other Information:
| |
---|
treatment | Two separate experiments were conducted to evaluate the effects of R:FR on transcriptome changes in the uppermost rosette bud (bud n) and the axillary bud in the axil of the third leaf from the top (bud n-2). WT Col-0 (CS60000) was used as the experimental material. Plants were grown individually in 26 by 60 mm tubes and watered and fertilized optimally. Plants were grown in a split growth chamber (providing uniform temperature and PPFD but\
allowing for differential R:FR) with 18 h photoperiods (185 microMoles m-2 s-1 PPFD provided by T12 VHO CW fluorescent lamps) and 24oC/18oC day/night temperatures. One day after sowing the R:FR was reduced on both sides of the chamber from 3.5 to 0.08 using FR LEDs fixed in clear overhead arrays. Prior to anthesis the plants were matched and split into two treatment groups. In experiment 1 the FR source for one of the groups was switched off at 12:00 pm on the\
day of anthesis, causing the R:FR to increase to 3.5. Unelongated axillary buds in the axil of the uppermost leaves (approx. 2.5 mm long) were harvested for RNA preparation from both groups (low and transiently increased R:FR) 3 h after changing the R:FR. Each treatment was composed of three biological replicates, each containing buds from about 15-18 plants. Experiment 2 was conducted exactly the same as experiment 1, except the R:FR was altered 3 days after \
anthesis and the unelongated axillary buds in the axils of the third leaves down (approx. 1 mm long) were harvested for RNA preparation.
This sample was derived from plants grown under low R:FR from 1 day after sowing until the first day of anthesis. At 12:00 on the day of anthesis, the plants were exposed to high R:FR for 3 h and rosette axillary bud n was harvested. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Finlayson_561-5_high-R:FR-bud-n_Rep2_ATH1 | | |
|
|
|
Tissue:
| Rosette axillary bud n |
---|
Other Information:
| |
---|
treatment | Two separate experiments were conducted to evaluate the effects of R:FR on transcriptome changes in the uppermost rosette bud (bud n) and the axillary bud in the axil of the third leaf from the top (bud n-2). WT Col-0 (CS60000) was used as the experimental material. Plants were grown individually in 26 by 60 mm tubes and watered and fertilized optimally. Plants were grown in a split growth chamber (providing uniform temperature and PPFD but\
allowing for differential R:FR) with 18 h photoperiods (185 microMoles m-2 s-1 PPFD provided by T12 VHO CW fluorescent lamps) and 24oC/18oC day/night temperatures. One day after sowing the R:FR was reduced on both sides of the chamber from 3.5 to 0.08 using FR LEDs fixed in clear overhead arrays. Prior to anthesis the plants were matched and split into two treatment groups. In experiment 1 the FR source for one of the groups was switched off at 12:00 pm on the\
day of anthesis, causing the R:FR to increase to 3.5. Unelongated axillary buds in the axil of the uppermost leaves (approx. 2.5 mm long) were harvested for RNA preparation from both groups (low and transiently increased R:FR) 3 h after changing the R:FR. Each treatment was composed of three biological replicates, each containing buds from about 15-18 plants. Experiment 2 was conducted exactly the same as experiment 1, except the R:FR was altered 3 days after \
anthesis and the unelongated axillary buds in the axils of the third leaves down (approx. 1 mm long) were harvested for RNA preparation.
This sample was derived from plants grown under low R:FR from 1 day after sowing until the first day of anthesis. At 12:00 on the day of anthesis, the plants were exposed to high R:FR for 3 h and rosette axillary bud n was harvested. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Finlayson_561-4_high-R:FR-bud-n_Rep1_ATH1 | | |
|
|
|
Tissue:
| Rosette axillary bud n |
---|
Other Information:
| |
---|
treatment | Two separate experiments were conducted to evaluate the effects of R:FR on transcriptome changes in the uppermost rosette bud (bud n) and the axillary bud in the axil of the third leaf from the top (bud n-2). WT Col-0 (CS60000) was used as the experimental material. Plants were grown individually in 26 by 60 mm tubes and watered and fertilized optimally. Plants were grown in a split growth chamber (providing uniform temperature and PPFD but\
allowing for differential R:FR) with 18 h photoperiods (185 microMoles m-2 s-1 PPFD provided by T12 VHO CW fluorescent lamps) and 24oC/18oC day/night temperatures. One day after sowing the R:FR was reduced on both sides of the chamber from 3.5 to 0.08 using FR LEDs fixed in clear overhead arrays. Prior to anthesis the plants were matched and split into two treatment groups. In experiment 1 the FR source for one of the groups was switched off at 12:00 pm on the\
day of anthesis, causing the R:FR to increase to 3.5. Unelongated axillary buds in the axil of the uppermost leaves (approx. 2.5 mm long) were harvested for RNA preparation from both groups (low and transiently increased R:FR) 3 h after changing the R:FR. Each treatment was composed of three biological replicates, each containing buds from about 15-18 plants. Experiment 2 was conducted exactly the same as experiment 1, except the R:FR was altered 3 days after \
anthesis and the unelongated axillary buds in the axils of the third leaves down (approx. 1 mm long) were harvested for RNA preparation.
This sample was derived from plants grown under low R:FR from 1 day after sowing until the first day of anthesis. At 12:00 on the day of anthesis, the plants were exposed to high R:FR for 3 h and rosette axillary bud n was harvested. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Finlayson_561-3_low-R:FR-bud-n_Rep3_ATH1 | | |
|
|
|
Tissue:
| Rosette axillary bud n |
---|
Other Information:
| |
---|
treatment | Two separate experiments were conducted to evaluate the effects of R:FR on transcriptome changes in the uppermost rosette bud (bud n) and the axillary bud in the axil of the third leaf from the top (bud n-2). WT Col-0 (CS60000) was used as the experimental material. Plants were grown individually in 26 by 60 mm tubes and watered and fertilized optimally. Plants were grown in a split growth chamber (providing uniform temperature and PPFD but allowing for differential R:FR) with 18 h photoperiods (185 microMoles m-2 s-1 PPFD provided by T12 VHO CW fluorescent lamps) and 24oC/18oC day/night temperatures. One day after sowing the R:FR was reduced on both sides of the chamber from 3.5 to 0.08 using FR LEDs fixed in clear overhead arrays. Prior to anthesis the plants were matched and split into two treatment groups. In experiment 1 the FR source for one of the groups was switched off at 12:00 pm on the\
day of anthesis, causing the R:FR to increase to 3.5. Unelongated axillary buds in the axil of the uppermost leaves (approx. 2.5 mm long) were harvested for RNA preparation from both groups (low and transiently increased R:FR) 3 h after changing the R:FR. Each treatment was composed of three biological replicates, each containing buds from about 15-18 plants. Experiment 2 was conducted exactly the same as experiment 1, except the R:FR was altered 3 days after \
anthesis and the unelongated axillary buds in the axils of the third leaves down (approx. 1 mm long) were harvested for RNA preparation.This sample was derived from plants maintained under low R:FR from 1 day after sowing until harvest. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Finlayson_561-2_low-R:FR-bud-n_Rep2_ATH1 | | |
|
|
|
Tissue:
| Rosette axillary bud n |
---|
Other Information:
| |
---|
treatment | Two separate experiments were conducted to evaluate the effects of R:FR on transcriptome changes in the uppermost rosette bud (bud n) and the axillary bud in the axil of the third leaf from the top (bud n-2). WT Col-0 (CS60000) was used as the experimental material. Plants were grown individually in 26 by 60 mm tubes and watered and fertilized optimally. Plants were grown in a split growth chamber (providing uniform temperature and PPFD but allowing for differential R:FR) with 18 h photoperiods (185 microMoles m-2 s-1 PPFD provided by T12 VHO CW fluorescent lamps) and 24oC/18oC day/night temperatures. One day after sowing the R:FR was reduced on both sides of the chamber from 3.5 to 0.08 using FR LEDs fixed in clear overhead arrays. Prior to anthesis the plants were matched and split into two treatment groups. In experiment 1 the FR source for one of the groups was switched off at 12:00 pm on the\
day of anthesis, causing the R:FR to increase to 3.5. Unelongated axillary buds in the axil of the uppermost leaves (approx. 2.5 mm long) were harvested for RNA preparation from both groups (low and transiently increased R:FR) 3 h after changing the R:FR. Each treatment was composed of three biological replicates, each containing buds from about 15-18 plants. Experiment 2 was conducted exactly the same as experiment 1, except the R:FR was altered 3 days after \
anthesis and the unelongated axillary buds in the axils of the third leaves down (approx. 1 mm long) were harvested for RNA preparation.This sample was derived from plants maintained under low R:FR from 1 day after sowing until harvest. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Finlayson_561-1_low-R:FR-bud-n_Rep1_ATH1 | | |
|
|
|
Tissue:
| Rosette axillary bud n |
---|
Additional Organism Information:
| |
---|
Sample Description | The sample was derived from Col-0 plants differentially treated and harvested on the day of anthesis |
---|
Other Information:
| |
---|
treatment | Two separate experiments were conducted to evaluate the effects of R:FR on transcriptome changes in the uppermost rosette bud (bud n) and the axillary bud in the axil of the third leaf from the top (bud n-2). WT Col-0 (CS60000) was used as the experimental material. Plants were grown individually in 26 by 60 mm tubes and watered and fertilized optimally. Plants were grown in a split growth chamber (providing uniform temperature and PPFD but allowing for differential R:FR) with 18 h photoperiods (185 microMoles m-2 s-1 PPFD provided by T12 VHO CW fluorescent lamps) and 24oC/18oC day/night temperatures. One day after sowing the R:FR was reduced on both sides of the chamber from 3.5 to 0.08 using FR LEDs fixed in clear overhead arrays. Prior to anthesis the plants were matched and split into two treatment groups. In experiment 1 the FR source for one of the groups was switched off at 12:00 pm on the
day of anthesis, causing the R:FR to increase to 3.5. Unelongated axillary buds in the axil of the uppermost leaves (approx. 2.5 mm long) were harvested for RNA preparation from both groups (low and transiently increased R:FR) 3 h after changing the R:FR. Each treatment was composed of three biological replicates, each containing buds from about 15-18 plants. Experiment 2 was conducted exactly the same as experiment 1, except the R:FR was altered 3 days after
anthesis and the unelongated axillary buds in the axils of the third leaves down (approx. 1 mm long) were harvested for RNA preparation. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Finlayson_561-11_high-R:FR-bud-n-2_Rep2_ATH1 | | |
|
|
|
Tissue:
| Rosette axillary bud n-2 |
---|
Other Information:
| |
---|
treatment | Two separate experiments were conducted to evaluate the effects of R:FR on transcriptome changes in the uppermost rosette bud (bud n) and the axillary bud in the axil of the third leaf from the top (bud n-2). WT Col-0 (CS60000) was used as the experimental material. Plants were grown individually in 26 by 60 mm tubes and watered and fertilized optimally. Plants were grown in a split growth chamber (providing uniform temperature and PPFD but\
allowing for differential R:FR) with 18 h photoperiods (185 microMoles m-2 s-1 PPFD provided by T12 VHO CW fluorescent lamps) and 24oC/18oC day/night temperatures. One day after sowing the R:FR was reduced on both sides of the chamber from 3.5 to 0.08 using FR LEDs fixed in clear overhead arrays. Prior to anthesis the plants were matched and split into two treatment groups. In experiment 1 the FR source for one of the groups was switched off at 12:00 pm on the\
day of anthesis, causing the R:FR to increase to 3.5. Unelongated axillary buds in the axil of the uppermost leaves (approx. 2.5 mm long) were harvested for RNA preparation from both groups (low and transiently increased R:FR) 3 h after changing the R:FR. Each treatment was composed of three biological replicates, each containing buds from about 15-18 plants. Experiment 2 was conducted exactly the same as experiment 1, except the R:FR was altered 3 days after \
anthesis and the unelongated axillary buds in the axils of the third leaves down (approx. 1 mm long) were harvested for RNA preparation.This sample was derived from plants grown under low R:FR from 1 day after sowing until 3 days after anthesis. At 12:00 on the third day after anthesis, the plants were exposed to high R:FR for 3 h and rosette axillary bud n-2 was harvested. |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Finlayson_561-12_high-R:FR-bud-n-2_Rep3_ATH1 | | |
|
|
|
Tissue:
| Rosette axillary bud n-2 |
---|
Other Information:
| |
---|
treatment | Two separate experiments were conducted to evaluate the effects of R:FR on transcriptome changes in the uppermost rosette bud (bud n) and the axillary bud in the axil of the third leaf from the top (bud n-2). WT Col-0 (CS60000) was used as the experimental material. Plants were grown individually in 26 by 60 mm tubes and watered and fertilized optimally. Plants were grown in a split growth chamber (providing uniform temperature and PPFD but\
allowing for differential R:FR) with 18 h photoperiods (185 microMoles m-2 s-1 PPFD provided by T12 VHO CW fluorescent lamps) and 24oC/18oC day/night temperatures. One day after sowing the R:FR was reduced on both sides of the chamber from 3.5 to 0.08 using FR LEDs fixed in clear overhead arrays. Prior to anthesis the plants were matched and split into two treatment groups. In experiment 1 the FR source for one of the groups was switched off at 12:00 pm on the\
day of anthesis, causing the R:FR to increase to 3.5. Unelongated axillary buds in the axil of the uppermost leaves (approx. 2.5 mm long) were harvested for RNA preparation from both groups (low and transiently increased R:FR) 3 h after changing the R:FR. Each treatment was composed of three biological replicates, each containing buds from about 15-18 plants. Experiment 2 was conducted exactly the same as experiment 1, except the R:FR was altered 3 days after \
anthesis and the unelongated axillary buds in the axils of the third leaves down (approx. 1 mm long) were harvested for RNA preparation.This sample was derived from plants grown under low R:FR from 1 day after sowing until 3 days after anthesis. At 12:00 on the third day after anthesis, the plants were exposed to high R:FR for 3 h and rosette axillary bud n-2 was harvested. |
---|
|
Protocols for BioSource 1 |
|
|
|
Problems? Comments? Suggestions? Contact the Affymetrix Team