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Experiment: Modulation of AMP1 expression and activity
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-652
To assess the transcriptomic effect of AMP1 overexpression we generated an estradiol-inducible AMP1 overexpression construct (ESR>>AMP1:MYC; Zuo et al., 2000) and brought it into the amp1-1 background. AMP1 encodes a putative glutamate carboxypeptidase and its loss of function results in increased shoot meristem activity. To identify short-time responses to increased AMP1 expression we induced ESR>>AMP1:MYC for 6h and 24h, respectively, and compared it to the DMSO treated mock control. We also included as control 6h DMSO-treated Col-0 and 6h DMSO-treated amp1-13 samples.In parallel we treated Col-0 plants for 6h, 24h and 10 days with chemical 32C8 which induces amp1-like phenotypes in wild-type and compared it tomock-treated wild-type and amp1-13 plants.Plants were grown under long-day conditions at 22°C and whole seedlings were used for RNA extraction.Zuo, J., Niu, Q. W.,&Chua, N. H. (2000) 24: 265-273
About the ExperimenterName: | Dr Tobias Sieberer |
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Head of Lab Name: | Dr Tobias Sieberer |
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Lab:
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Address: | Department of Microbiology, Immunobiology and Genetics, Max F. Perutz Laboratories, University of Vienna Dr. Bohrgasse 9/4, Wien, Vienna, 1030, AUSTRIA
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Postcode:
| 1030 |
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Country:
| AUSTRIA
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| compound_based_treatment |
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Number of Slides: | 11 |
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| Experimental Parameters:
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parameter | genetic_modification |
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Quality Control Measures Taken:
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References:
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Reference | Zuo J, Niu QW, Chua NH. An estrogen receptor-based transactivator XVE mediates highly inducible gene expression in transgenic plants. Plant J. 2000;24:265-273 |
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Sieberer: Modulation of AMP1 expression and activity
Slide: Sieberer_652-1_WT+DMSO_6h_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| At 9th day the whole seedlings were taken out of agar medium and placed into a liquid growth medium (the same medium just without agar) and incubated in such conditions for 1 day.
At the next day (10th day) dimethyl sulfoxide (10µl DMSO/1ml of liquid medium) for 6 hours was added. After the DMSO treatment seedlings were frozen in liquid nitrogen and transferred to -80C. |
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Additional Organism Information:
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Sample Description | Healthy plant |
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Other Information:
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Timecourse start procedure | |
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Protocols for BioSource 1 |
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Slide: Sieberer_652-2_WT+DMSO_const_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| DMSO control treatment 10 µl/ml |
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Additional Organism Information:
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Sample Description | Healthy plant |
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Other Information:
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Timecourse start procedure | |
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Protocols for BioSource 1 |
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Slide: Sieberer_652-3_Line1+DMSO_6h_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| At 9th day the whole seedlings were taken out of agar medium and placed into a liquid growth medium (the same medium just without agar) and incubated in such conditions for 1 day.
At the next day (10th day) dimethyl sulfoxide (10µl DMSO/1ml of liquid medium) for 6 hours was added. After the DMSO treatment seedlings were frozen in liquid nitrogen and transferred to -80C. |
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Additional Organism Information:
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Sample Description | Healthy plant |
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Other Information:
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Timecourse start procedure | |
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Protocols for BioSource 1 |
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Slide: Sieberer_652-4_Line1+DMSO_24h_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| At 9th day the whole seedlings were taken out of agar medium and placed into a liquid growth medium (the same medium just without agar) and incubated in such conditions for 1 day.
At the next day (10th day) dimethyl sulfoxide (10µl DMSO/1ml of liquid medium) for 24 hours was added. After the DMSO treatment seedlings were frozen in liquid nitrogen and transferred to -80C. |
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Additional Organism Information:
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Sample Description | Healthy plant |
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Other Information:
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Timecourse start procedure | |
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Protocols for BioSource 1 |
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Slide: Sieberer_652-5_Line1+ES_6h_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| At 9th day the whole seedlings were taken out of agar and placed into a liquid growth medium (the same medium just without agar) and incubated in such conditions for 1 day.
At the next day (10th day) estradiol (50µM) for 6 hours was added. After the estradiol treatment seedlings were frozen in liquid nitrogen and transferred to -80C. |
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Additional Organism Information:
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Sample Description | Healthy plant |
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Other Information:
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Timecourse start procedure | |
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Protocols for BioSource 1 |
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Slide: Sieberer_652-6_Line1+ES_24h_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| At 9th day the whole seedlings were taken out of agar and placed into a liquid growth medium (the same medium just without agar) and incubated in such conditions for 1 day.
At the next day (10th day) estradiol (50µM) for 24 hours was added. After the estradiol treatment seedlings were frozen in liquid nitrogen and transferred to -80C. |
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Additional Organism Information:
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Sample Description | Healthy plant |
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Other Information:
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Timecourse start procedure | |
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Protocols for BioSource 1 |
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Slide: Sieberer_652-7_amp1.13+DMSO_6h_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| At 9th day the whole seedlings were taken out of agar medium and placed into a liquid growth medium (the same medium just without agar) and incubated in such conditions for 1 day.
At the next day (10th day) dimethyl sulfoxide (10µl DMSO/1ml of liquid medium) for 6 hours was added. After the DMSO treatment seedlings were frozen in liquid nitrogen and transferred to -80C. |
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Additional Organism Information:
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Sample Description | Healthy plant |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Sieberer_652-8_amp1.13+DMSO_const_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| DMSO control treatment (10µl/ml) |
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Additional Organism Information:
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Sample Description | Healthy plant |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Sieberer_652-9_WT+32_C8_6h_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| At 9th day the whole seedlings were taken out of agar and placed into a liquid growth medium (the same medium just without agar) and incubated in such conditions for 1 day.
At the next day (10th day) chemical 32C8 (50 µM) for 6 hours was added. After the 32C8 treatment seedlings were frozen in liquid nitrogen and transferred to -80C. |
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Additional Organism Information:
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Sample Description | Healthy plant |
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Other Information:
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Timecourse start procedure | |
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Protocols for BioSource 1 |
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Slide: Sieberer_652-10_WT+32_C8_24h_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| At 9th day the whole seedlings were taken out of agar and placed into a liquid growth medium (the same medium just without agar) and incubated in such conditions for 1 day.
At the next day (10th day) chemical 32C8 (50 µM) for 24 hours was added. After the 32C8 treatment seedlings were frozen in liquid nitrogen and transferred to -80C. |
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Additional Organism Information:
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Sample Description | Healthy plant |
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Other Information:
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Timecourse start procedure | |
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Protocols for BioSource 1 |
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Slide: Sieberer_652-11_WT+32_C8_const_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| chemical 32C8 50µM |
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Additional Organism Information:
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Sample Description | Healthy plant |
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Other Information:
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Protocols for BioSource 1 |
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