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Experiment: Functional Genomics of Ozone Stress in Arabidopsis.
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-26
Ozone is known to induce gene expression in plants. The roles of the induced genes and the molecular basis for the induction however largely remain to be elucidated. We will expose 2 week-old Arabidopsis seedlings to 200 ppb ozone for 1 h. RNA will be extracted from control and ozone-fumigated seedlings 3 h following the end of the fumigation period. The induction of the known anti-oxidant enzyme GST will be confirmed prior to submission of the RNA samples to GARNet. Changes in gene expression will be assessed by hybridising microarrays with fluorescently labelled cDNA prepared from control and ozone-fumigated seedlings. The role of selected genes will be inferred from their sequence and further established by over expression under the control of the 35S or cell-specific promoters and by searching for mutants among single transposon or T-DNA insertion collections. Plants will be analysed on the basis of traits that are known to be affected by ozone including growth, flowering and stomatal responses. In addition, the ozone calcium signature we have reported previously (Clayton et al.1999) will be studied by crossing transgenic or mutant plants with apoaequorin or yellow cameleon 2.1-transformed plants.
About the ExperimenterName: | Miss Eleri Short |
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Head of Lab Name: | Dr Alan Shirras |
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Lab:
| Department of Biological Sciences |
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Institute:
| Lancaster University |
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Address: | Department of Biological Sciences Lancaster University Lancaster
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Postcode:
| LA1 4YQ |
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Country:
| UK |
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| Telephone Number:
| 01524 593929 |
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Fax Number:
| 01524 843854 |
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| URL for this person/group: | http://biol.lancs.ac.uk/ADS/ads.html |
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are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| compound_treatment_design |
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Number of Slides: | 6 |
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| Experimental Parameters:
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Parameter | compound_based_treatment |
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Quality Control Measures Taken:
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Short_genome
Slide: Short_1-1_ozone_Rep1_ATH1 | | |
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Tissue:
| Whole Plant |
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Diseased:
| Normal |
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in vivo Treatment:
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Treatment | Fumigation with 500ppb Ozone for 6 hours from 10.30am in sealed chambers inside the growth cabinet. Flow rate was 910ml/min. The ozone was bubbled through water before entering fumigation chambers to humidify the gas. Seelings were fumigated in their growth plates and extracted immediately after fumigation. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Short_1-2_control_Rep1_ATH1 | | |
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Tissue:
| Whole Plant |
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Diseased:
| Normal |
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in vivo Treatment:
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Treatment | Fumigation with scrubbed air (filtered through charcoal and purafill) for 6 hours from 10.30am in sealed chambers inside the growth cabinet. Flow rate was 910ml/min. The air was bubbled through water before entering fumigation chambers to humidify the gas. Seelings were fumigated in their growth plates and RNA extracted immediately after fumigation. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Short_1-3_ozone_Rep2_ATH1 | | |
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Tissue:
| Whole Plant |
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Diseased:
| Normal |
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in vivo Treatment:
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Treatment | Fumigation with 500ppb Ozone for 6 hours from 10.30am in sealed chambers inside the growth cabinet. Flow rate was 910ml/min. The ozone was bubbled through water before entering fumigation chambers to humidify the gas. Seelings were fumigated in their growth plates and extracted immediately after fumigation. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Short_1-5_ozone_Rep3_ATH1 | | |
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Tissue:
| Whole Plant |
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Diseased:
| Normal |
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in vivo Treatment:
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Treatment | Fumigation with 500ppb Ozone for 6 hours from 10.30am in sealed chambers inside the growth cabinet. Flow rate was 910ml/min. The ozone was bubbled through water before entering fumigation chambers to humidify the gas. Seelings were fumigated in their growth plates and extracted immediately after fumigation. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Short_1-4_control_Rep2_ATH1 | | |
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Tissue:
| Whole Plant |
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Diseased:
| Normal |
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in vivo Treatment:
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Treatment | Fumigation with scrubbed air (filtered through charcoal and purafill) for 6 hours from 10.30am in sealed chambers inside the growth cabinet. Flow rate was 910ml/min. The air was bubbled through water before entering fumigation chambers to humidify the gas. Seelings were fumigated in their growth plates and RNA extracted immediately after fumigation. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Short_1-6_control_Rep3_ATH1 | | |
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Tissue:
| Whole Plant |
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Diseased:
| Normal |
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in vivo Treatment:
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Treatment | Fumigation with scrubbed air (filtered through charcoal and purafill) for 6 hours from 10.30am in sealed chambers inside the growth cabinet. Flow rate was 910ml/min. The air was bubbled through water before entering fumigation chambers to humidify the gas. Seelings were fumigated in their growth plates and RNA extracted immediately after fumigation. |
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Other Information:
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Protocols for BioSource 1 |
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