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Experiment: Cold acclimation in the sfr3-1 mutant
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-383
The sfr3-1 mutation causes freezing-sensitivity in Arabidopsis thaliana. The mutated gene has been identified by positional cloning and is currently being characterised. The mutant appears normal when grown in the warm (no phenotype has been identified associated with such growth). However, following cold acclimation and subsequent freezing mutant plants are severely damaged whilst wild type plants are not. This suggests that sfr3 is deficient in the cold acclimation process. Micro-array analysis will enable the identification of any transcriptional changes during the cold acclimation process. This information will then be used, together with information obtained by gene characterisation, in order to more fully understand the nature of the sfr3 mutation.
About the ExperimenterName: | Dr Sarah Garton |
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Head of Lab Name: | Dr Glenn Thorlby |
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Lab:
| Thorlby Lab |
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Institute:
| Royal Holloway University of London |
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Address: | School of Biological Sciences Royal Holloway University of London Egham Hill Egham Surrey
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Postcode:
| TW20 0EX |
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Country:
| UK |
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| Telephone Number:
| 01784443770 |
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Fax Number:
| 01784434326 |
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are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| genetic_modification_design; growth_condition_design; |
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Number of Slides: | 8 |
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| Experimental Parameters:
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parameter | gene_knock_out |
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parameter | change_temperature |
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Quality Control Measures Taken:
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Garton: Cold acclimation in the sfr3-1 mutant_genome
Slide: Garton_1-1_sfr3-warm_Rep1_ATH1 | | |
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Tissue:
| aerial tissues |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Garton_1-2_sfr3-cold_Rep1_ATH1 | | |
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Tissue:
| aerial tissues |
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in vivo Treatment:
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Treatment protocol | Following 28 days growth in short day conditions at 20 degrees centigrade, the plants were transferred to 4 degrees centigrade for 10 days prior to sampling. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Garton_1-3_sfr3-warm_Rep2_ATH1 | | |
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Tissue:
| aerial tissues |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Garton_1-4_sfr3-cold_Rep2_ATH1 | | |
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Tissue:
| aerial tissues |
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in vivo Treatment:
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Treatment protocol | Following 28 days growth in short day conditions at 20 degrees centigrade, the plants were transferred to 4 degrees centigrade for 10 days prior to sampling. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Garton_1-5_wildtype-warm_Rep1_ATH1 | | |
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Tissue:
| aerial tissues |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Garton_1-6_wildtype-cold_Rep1_ATH1 | | |
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Tissue:
| aerial tissues |
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in vivo Treatment:
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Treatment protocol | Following 28 days growth in short day conditions at 20 degrees centigrade, the plants were transferred to 4 degrees centigrade for 10 days prior to sampling. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Garton_1-7_wildtype-warm_Rep2_ATH1 | | |
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Tissue:
| aerial tissues |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Garton_1-8_wildtype-cold_Rep2_ATH1 | | |
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Tissue:
| aerial tissues |
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in vivo Treatment:
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Treatment protocol | Following 28 days growth in short day conditions at 20 degrees centigrade, the plants were transferred to 4 degrees centigrade for 10 days prior to sampling. |
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Other Information:
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Protocols for BioSource 1 |
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Problems? Comments? Suggestions? Contact the Affymetrix Team