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Experiment: Chemical compound induced somatic embryogenesis in Arabidopsis
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-584
Dry seed of wild-type columbia were germinated in containers with liquid 0.5ms including 1µM 2.4-D.After two days the seedlings were induced with 1µM of 2,4-D incombination with 25µM of the chemical compound. As a control we used seedlings incubated with DMSO.After 0.5hr induction the seedlings were harvested.RNA was extracted using the RNA isolation kit of invitek with an on-column DNAse treatment during the RNA isolation.
About the ExperimenterName: | Mr Tom Stekelenburg |
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Head of Lab Name: | Gerco Angenent |
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Lab:
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Address: | Business Unit Bioscience, Plant Research International B.V., Wageningen University Post box nr 619, Wageningen, , 6700 AP, NETHERLANDS
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Postcode:
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Country:
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| Telephone Number:
| 0317-480889 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| compound_based_treatment |
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Number of Slides: | 6 |
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| Experimental Parameters:
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parameter | compound_based_treatment |
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Quality Control Measures Taken:
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Stekelenburg: Chemical compound induced somatic embryogenesis in Arabidopsis
Slide: Stekelenburg_584-6_compound_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| After 2 days 25µM Chemical compound was added. 1µM 2.4-D was present from the beginning.
seedlings were harvested after 0.5hr of induction. |
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Additional Organism Information:
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Sample Description | RNA was isolated from whole 2 day old seedlings. |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Stekelenburg_584-5_compound_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| After 2 days 25µM Chemical compound was added. 1µM 2.4-D was present from the beginning.
seedlings were harvested after 0.5hr of induction. |
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Additional Organism Information:
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Sample Description | RNA was isolated from whole 2 day old seedlings. |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Stekelenburg_584-4_compound_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| After 2 days 25µM Chemical compound was added. 1µM 2.4-D was present from the beginning.
seedlings were harvested after 0.5hr of induction. |
---|
Additional Organism Information:
| |
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Sample Description | RNA was isolated from whole 2 day old seedlings. |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Stekelenburg_584-3_cont_DMSO_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| After 2 days DMSO was added. 1µM 2.4-D was present from the beginning.
seedlings were harvested after 0.5hr of induction. |
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Additional Organism Information:
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Sample Description | RNA was isolated from whole 2 day old seedlings. |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Stekelenburg_584-1_cont_DMSO_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| After 2 days DMSO was added. 1µM 2.4-D was present from the beginning.
seedlings were harvested after 0.5hr of induction. |
---|
Additional Organism Information:
| |
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Sample Description | RNA was isolated from whole 2 day old seedlings. |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Stekelenburg_584-2_cont_DMSO_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| After 2 days DMSO was added. 1µM 2.4-D was present from the beginning.
seedlings were harvested after 0.5hr of induction. |
---|
Additional Organism Information:
| |
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Sample Description | RNA was isolated from whole 2 day old seedlings. |
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Other Information:
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---|
ecotype_habitat | |
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Protocols for BioSource 1 |
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