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Experiment: Over-expression of MBF1c enhances stress tolerance
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-335
Multiprotein bridging factor 1c MBF1c (At3g24500) is a stress-response transcription co-activator. To test the function of MBF1c, we over-expressed it in transgenic Arabidopsis plants using the 35S-CaMV promoter. T4 seeds form 3 independent lines were tested for their tolerance to biotic and abiotic stress conditions. Constitutive expression of MBF1c in Arabidopsis enhanced the tolerance of transgenic plants to bacterial infection, salinity, heat and osmotic stress. Moreover, the enhanced tolerance of transgenic plants to osmotic and heat stress was maintained even when these two stresses were combined. The expression of MBF1c in transgenic plants augmented the accumulation of a number of sugars and defense transcrtipts in response to heat stress. Transcriptome profiling and inhibitor studies suggest that MBF1c expression enhances the tolerance of transgenic plants to heat and osmotic stress by partially activating, or perturbing, the ethylene-response signal transduction pathway. MBF1 proteins could be used to enhance the tolerance of plants to different abiotic stresses. Suzuki et al., 2005 Plant Physiology, submitted.
About the ExperimenterName: | Dr Ron Mittler |
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Head of Lab Name: | Dr Ron Mittler |
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Lab:
| Dept. of Biochemistry |
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Institute:
| University of Nevada |
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Address: | MS200 Reno Nevada
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Postcode:
| 89557 |
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Country:
| USA |
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| Telephone Number:
| 1-775-784-1384 |
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Fax Number:
| 1-775-784-1650 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| genetic_modification_design; stimulus_or_stress_design; compound_based_treatment_design |
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Number of Slides: | 6 |
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| Experimental Parameters:
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parameter | gene_knock_out |
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parameter | compound_based_treatment |
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Quality Control Measures Taken:
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References:
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reference | Suzuki et al., 2005 Plant Physiology, submitted |
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| Other Information:
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GEO accession | GSE5539 |
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Slides in this Experiment
Hybridisation Set: Mittler genome
Slide: Mittler_1-1_control_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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Diseased:
| Normal |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Mittler_1-2_control_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Diseased:
| Normal |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Mittler_1-3_control_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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Diseased:
| Normal |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Mittler_1-4_MBF1c_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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Diseased:
| Normal |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Mittler_1-5_MBF1c_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Diseased:
| Normal |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Mittler_1-6_MBF1c_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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Diseased:
| Normal |
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Other Information:
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Protocols for BioSource 1 |
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