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Experiment: Transcriptome analysis during axillary shoot outgrowth
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-498
In plant axillary bud dormancy and outgrowth are regulated by phytohoromones, but it is still unknown about its molecular mechanism. We reveal that Arabidopsis axillary buds located at axil of rosette leaves show dormancy and that this is broken by the decapitation of main stem, resulting in the bud outgrowth. To investigate about the molecular mechanisms of dormancy and outgrowth, we carried out gene expression analysis during axillary shoot outgrowth in Arabidopsis wild type Columbia accession. Since axillary buds did not initiate outgrowth (dormancy) at 5 day after bolting of main stem, we used 5-day bolted plants as a control (before decapitation). Then, main stems were decapitated, and axillary shoots were collected at 24 hours after decapitation (named as growing shoot). Total RNA was prepared from either control or growing shoots and used for the microarray analysis. We carried out duplicate microarray analysis using independent plant materials.Ref):Tatematsu et al., Plant Physiol. 138: 757-766 (2005).
About the ExperimenterName: | Kiyoshi Tatematsu |
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Head of Lab Name: | Eiji Nambara |
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Lab:
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Address: | Department of Cell and System Biology University of Toronto 25 Willcocks St. Toronto Ontario
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Postcode:
| M5S 3B2 |
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Country:
| Canada |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| organism_part_comparison_design; growth_condition_design; |
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Number of Slides: | 4 |
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| Experimental Parameters:
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parameter | organism_part |
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parameter | grow |
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Quality Control Measures Taken:
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References:
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Reference | Tatematsu et al., Plant Physiol. 138: 757-766 (2005) |
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Slides in this Experiment
Hybridisation Set: Tatematsu: Transcriptome analysis during axillary shoot outgrowth_genome
Slide: Tatematsu_2-1_axillary-buds-before-decapitation_Rep1_ATH1 | | |
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Tissue:
| axillary buds |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Tatematsu_2-2_axillary-buds-before-decapitation_Rep2_ATH1 | | |
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Tissue:
| axillary buds |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Tatematsu_2-3_axillary-shoots-at-24-h-after-decapitation_Rep1_ATH1 | | |
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Tissue:
| axillary shoots |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Tatematsu_2-4_axillary-shoots-at-24-h-after-decapitation_Rep2_ATH1 | | |
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Tissue:
| axillary shoots |
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Other Information:
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Protocols for BioSource 1 |
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