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Experiment: Expression analysis in HDA6 mutants
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-478
In addition to the canonical RNAi pathways that targets mRNAs in the cytoplasm, several RNA-dependent pathways operate in the nucleus to induce sequence-specific epigenetic modifications. One specialized type of RNAi-mediated pathway is RNA-directed DNA methylation (RdDM). During RdDM, nuclear DNA with sequence identity to the trigger dsRNA is de novo methylated at almost all cytosine residues, providing a mark for the formation of transcriptionally silent heterochromatin. Genetic forward screens identified the RPD3-like histone deacetylase HDA6 as the enzyme responsible for the histone deacetylation step of RdDM and suggest that HDA6 might have acquired specific functions for RNA-directed transcriptional silencing processes [Aufsatz et al., 2002a; Aufsatz et al., 2002b].Complete loss-of-function mutants for AtHDA6 (rts1-1; RNA-mediated transcriptional gene silencing) exhibit reactivation of RdDM-silenced promoters, despite the continuous presence of the silencing-inducing RNA signal. One of the found mutant alleles, rts1-5, has an amino acid substitution located at a conserved position within the HDAC domain (Naumann et al., manuscript in preparation). This mutant is characterized by strong reactivation of an RdDM-silenced target promoter, despite maintaining wild-type levels of cytosine methylation.An evaluation of the transcription pattern among the mutants with opposite methylation phenotype and wild-type plants should show which genes are differentially regulated between the mutants in comparison to wild-type plants.
About the ExperimenterName: | Franziska Schuller |
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Head of Lab Name: | Dr Werner Aufsatz |
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Lab:
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Address: | GMI - Gregor Mendel Institute of Molecular Plant Biology GmbH Dr. Bohr-Gasse 3
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Postcode:
| A-1030 Wien |
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Country:
| Austria |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
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co-authors in the work.
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| About this ExperimentExperiment Type:
| genetic_modification_design |
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Number of Slides: | 9 |
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| Experimental Parameters:
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Quality Control Measures Taken:
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Slides in this Experiment
Hybridisation Set: Schuller: Expression analysis in HDA6 mutants_genome
Slide: Schuller_1-1_double-transformant_Rep1_ATH1_rpt | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Schuller_1-3_double-transformant_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Schuller_1-2_double-transformant_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Schuller_1-4_null-mutant_Rep1_ATH1_rpt | | |
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Tissue:
| whole plant |
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Sample Description | |
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Protocols for BioSource 1 |
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Slide: Schuller_1-5_null-mutant_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Sample Description | |
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Protocols for BioSource 1 |
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Slide: Schuller_1-6_null-mutant_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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Sample Description | |
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Protocols for BioSource 1 |
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Slide: Schuller_1-7_amino-acid-substitution_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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Sample Description | |
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Protocols for BioSource 1 |
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Slide: Schuller_1-8_amino-acid-substitution_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | |
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Protocols for BioSource 1 |
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Slide: Schuller_1-9_amino-acid-substitution_Rep3_ATH1_rpt | | |
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Tissue:
| whole plant |
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Sample Description | |
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Other Information:
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Protocols for BioSource 1 |
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