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Experiment: Photoreceptor control of shoot meristem activity and leaf initiation
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-426
When Arabidopsis seedlings are grown in the dark, their shoot meristem remains in a repressed state and does not develop leaves. The tight control of leaf development by light provides the opportunity to analyse a fundamental plant developmental process in its very early stages. We have observed that activation of photoreceptors in dark-grown seedlings leads to a rapid and dramatic increase in mitotic activity in and around the shoot meristem. Cotyledons also exhibit an induction of cell cycle activity by light, but this consists of endoreduplication and occurs later. Many other processes take place upon induction by light, including the development of the photosynthetic apparatus and the synthesis of sunscreens, but these processes are expected to take place in both the leaf primordia and the cotyledons. We have used this information to carry out a whole transcriptome analysis of shoot meristem activation and early stages of leaf development by light. For this we dissected the shoot apical regions from seedlings grown in the dark or 1, 2, 6, 24, 48 or 72 h after transfer to light. We also dissected cotyledons from equivalent seedlings grown in the dark or 1 or 6 h after transfer to light. From these samples we obtained RNA used for hybridisations against the ATH1 GenChip.
About the ExperimenterName: | Dr Enrique Lopez-Juez |
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Head of Lab Name: | Dr Enrique Lopez-Juez |
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Lab:
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Address: | School of Biological Sciences Royal Holloway, University of London Egham Surrey
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Postcode:
| TW20 0EX |
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Country:
| UK |
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| Telephone Number:
| 01784443951 |
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Fax Number:
| 01784 414224 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| time_series_design; growth_condition_design; organism_part_comparison_design |
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Number of Slides: | 16 |
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| Experimental Parameters:
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parameter | timepoint |
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parameter | organism_part |
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parameter | change_light |
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Quality Control Measures Taken:
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References:
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| Other Information:
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GEO Accession | GSE19261 |
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Slides in this Experiment
Hybridisation Set: Lopez-Juez: Photoreceptor control of shoot meristem activity and leaf initiation_genome
Slide: Lopez-Juez_1-1_Shoot-apex-dark_Rep1_ATH1 | | |
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Tissue:
| shoot apical meristem region |
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Additional Organism Information:
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sample description | 3 day old, dark-grown seedling. Approximately 10 mm long, folded cotyledons. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Lopez-Juez_1-2_Shoot-apex-dark_Rep2_ATH1 | | |
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Tissue:
| shoot apical meristem region |
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Additional Organism Information:
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sample description | 3 day old, dark-grown seedling. Approximately 10 mm long, folded cotyledons. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Lopez-Juez_1-3_Shoot-apex-1hr-light_Rep1_ATH1 | | |
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Tissue:
| shoot apical meristem region |
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in vivo Treatment:
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Treament | Light (transfer from the dark) |
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Additional Organism Information:
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sample description | 3 day old, dark-grown seedling, after 1 h in the light. Approximately 10 mm long, folded cotyledons. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Lopez-Juez_1-4_Shoot-apex-1hr-light_Rep1_ATH1 | | |
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Tissue:
| shoot apical meristem region |
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in vivo Treatment:
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Treament | Light (transfer from the dark) |
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Additional Organism Information:
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sample description | 3 day old, dark-grown seedling, after 1 h in the light. Approximately 10 mm long, folded cotyledons. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Lopez-Juez_1-5_Shoot-apex-2hr-light_Rep1_ATH1 | | |
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Tissue:
| shoot apical meristem region |
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in vivo Treatment:
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Treament | Light (transfer from the dark) |
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Additional Organism Information:
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sample description | 3 day old, dark-grown seedling, after 2 h in the light. Approximately 10 mm long, folded cotyledons. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Lopez-Juez_1-6_Shoot-apex-6hr-light_Rep1_ATH1 | | |
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Tissue:
| shoot apical meristem region |
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in vivo Treatment:
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Treament | Light (transfer from the dark) |
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Additional Organism Information:
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sample description | 3 day old, dark-grown seedling, after 6 h in the light. Approximately 10 mm long, cotyledons unfolding and starting to green. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Lopez-Juez_1-7_Shoot-apex-6hr-light_Rep2_ATH1 | | |
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Tissue:
| shoot apical meristem region |
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in vivo Treatment:
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Treament | Light (transfer from the dark) |
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Additional Organism Information:
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sample description | 3 day old, dark-grown seedling, after 6 h in the light. Approximately 10 mm long, cotyledons unfolding and starting to green. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Lopez-Juez_1-8_Shoot-apex-24hr-light_Rep1_ATH1 | | |
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Tissue:
| shoot apical meristem region |
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in vivo Treatment:
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Treament | Light (transfer from the dark) |
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Additional Organism Information:
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sample description | 4 day old seedling (3 days in the dark, one in the light). Approximately 10 mm long, cotyledons unfolded and green. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Lopez-Juez_1-9_Shoot-apex-48hr-light_Rep1_ATH1 | | |
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Tissue:
| shoot apical meristem region and developing leaf primordia |
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in vivo Treatment:
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Treament | Light (transfer from the dark) |
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Additional Organism Information:
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sample description | 5 day old seedling (3 days in the dark, two in the light). Approximately 10 mm long, cotyledons unfolded and green, leaf primordia visible. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Lopez-Juez_1-10_Shoot-apex-72hr-light_Rep1_ATH1 | | |
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Tissue:
| shoot apical meristem region and developing leaf primordia |
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in vivo Treatment:
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Treament | Light (transfer from the dark) |
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Additional Organism Information:
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sample description | 6 day old seedling (3 days in the dark, 3 in the light). Approximately 10 mm long, cotyledons unfolded and green, very young leaves emerging. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Lopez-Juez_1-11_Cotyledons-dark_Rep1_ATH1 | | |
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Tissue:
| Cotyledon, with both the cotyledon petiole and the cotyledon tip removed |
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Additional Organism Information:
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sample description | 3 day old seedling. Approximately 10 mm long, cotyledons folded. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Lopez-Juez_1-12_Cotyledons-dark_Rep2_ATH1 | | |
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Tissue:
| Cotyledon, with both the cotyledon petiole and the cotyledon tip removed |
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Additional Organism Information:
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sample description | 3 day old dark-grown seedling. Approximately 10 mm long, cotyledons folded. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Lopez-Juez_1-13_Cotyledons-1hr-light_Rep1_ATH1 | | |
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Tissue:
| Cotyledon, with both the cotyledon petiole and the cotyledon tip removed |
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in vivo Treatment:
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Treament | Light (transfer from the dark) |
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Additional Organism Information:
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sample description | 3 day old dark-grown seedling, after only 1 h in the light. Approximately 10 mm long, cotyledons folded. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Lopez-Juez_1-14_Cotyledons-1hr-light_Rep2_ATH1 | | |
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Tissue:
| Cotyledon, with both the cotyledon petiole and the cotyledon tip removed |
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in vivo Treatment:
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Treament | Light (transfer from the dark) |
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Additional Organism Information:
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sample description | 3 day old dark-grown seedling, after only 1 h in the light. Approximately 10 mm long, cotyledons folded. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Lopez-Juez_1-15_Cotyledons-6hr-light_Rep1_ATH1 | | |
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Tissue:
| Cotyledon, with both the cotyledon petiole and the cotyledon tip removed |
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in vivo Treatment:
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Treament | Light (transfer from the dark) |
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Additional Organism Information:
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sample description | 3 day old dark-grown seedling, after 6 h in the light. Approximately 10 mm long, cotyledons unfolding and starting to green. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Lopez-Juez_1-16_Cotyledons-6hr-light_Rep2_ATH1 | | |
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Tissue:
| Cotyledon, with both the cotyledon petiole and the cotyledon tip removed |
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in vivo Treatment:
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Treament | Light (transfer from the dark) |
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Additional Organism Information:
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sample description | 3 day old dark-grown seedling, after 6 h in the light. Approximately 10 mm long, cotyledons unfolding and starting to green. |
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Other Information:
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Protocols for BioSource 1 |
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Problems? Comments? Suggestions? Contact the Affymetrix Team