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Experiment: WT vs NR null mutant high nitrate concn treatment
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-480
A nitrate reductase (NR)-null mutant of Arabidopsis was constructed that had a deletion of the major NR gene NIA2 and an insertion in the NIA1 NR gene. This mutant had no detectable NR activity and could not use nitrate as the sole nitrogen source. Starch mobilization was not induced by nitrate in this mutant but was induced by ammonium, indicating that nitrate was not the signal for this process. Microarray analysis of gene expression revealed that 595 genes responded to nitrate (5 mM nitrate for 2 h) in both wild-type and mutant plants. This group of genes was overrepresented most significantly in the functional categories of energy, metabolism, and glycolysis and gluconeogenesis. Because the nitrate response of these genes was NR independent, nitrate and not a downstream metabolite served as the signal. The microarray analysis also revealed that shoots can be as responsive to nitrate as roots, yet there was substantial organ specificity to the nitrate response.
About the ExperimenterName: | Dr Rongchen Wang |
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Head of Lab Name: | Professor Nigel Crawford |
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Lab:
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Address: | University of California, San Diego Division of Biological Sciences 9500 Gilman Drive, 0116 La Jolla, CA 92093-0116 USA
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Postcode:
| 92093-0116 |
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Country:
| USA |
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| Telephone Number:
| 18585342519 |
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Fax Number:
| 18585347108 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| compound_treatment_design; genetic_modification_design |
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Number of Slides: | 16 |
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| Experimental Parameters:
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parameter | compound_based_treatment |
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parameter | gene_knock_out |
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Quality Control Measures Taken:
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References:
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Reference | Plant Physiol. 2004 September; 136(1): 2512-2522. |
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Wang: WT vs NR null mutant high nitrate concn treatment_genome
Slide: Wang_2-16_Wild-type-Col-5-mM-KNO3-2-hr-shoots_Rep2_ATH1 | | |
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Tissue:
| shoots |
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Diseased:
| Treatment |
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in vivo Treatment:
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Treatment | 5 mM KNO3 |
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Additional Organism Information:
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Sample description | Wild type Col, 5 mM KNO3, 2 hr, shoots, replicate 2 |
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Other Information:
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Reference | Plant Physiol. 2004 September; 136(1): 2512-2522 |
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Protocols for BioSource 1 |
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Slide: Wang_2-15_Wild-type-Col-5-mM-KCL-2-hr-shoots_Rep2_ATH1 | | |
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Tissue:
| shoots |
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Diseased:
| Control |
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in vivo Treatment:
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Treatment | 5 mM KCl |
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Additional Organism Information:
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Sample description | Wild type Col, 5 mM KCL, 2 hr, shoots, replicate 2 |
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Other Information:
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Reference | Plant Physiol. 2004 September; 136(1): 2512-2522 |
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Protocols for BioSource 1 |
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Slide: Wang_2-13_Wild-type-Col-5-mM-KCL-2-hr-shoots_Rep1_ATH1 | | |
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Tissue:
| shoots |
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Diseased:
| Control |
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in vivo Treatment:
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Treatment | 5 mM KCl |
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Additional Organism Information:
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Sample description | Wild type Col, 5 mM KCL, 2 hr, shoots, replicate 1 |
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Other Information:
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Reference | Plant Physiol. 2004 September; 136(1): 2512-2522 |
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Protocols for BioSource 1 |
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Slide: Wang_2-14_Wild-type-Col-5-mM-KNO3-2-hr-shoots_Rep1_ATH1 | | |
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Tissue:
| shoots |
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Diseased:
| Treatment |
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in vivo Treatment:
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Treatment | 5 mM KNO3 |
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Additional Organism Information:
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Sample description | Wild type Col, 5 mM KNO3, 2 hr, shoots, replicate 1 |
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Other Information:
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Reference | Plant Physiol. 2004 September; 136(1): 2512-2522 |
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Protocols for BioSource 1 |
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Slide: Wang_2-11_Wild-type-Col-5-mM-KCL-2-hr-roots_Rep2_ATH1 | | |
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Tissue:
| Roots |
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Diseased:
| Control |
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in vivo Treatment:
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Treatment | 5 mM KCl |
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Additional Organism Information:
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Sample description | Wild type Col, 5 mM KCL, 2 hr, roots, replicate 2 |
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Other Information:
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Reference | Plant Physiol. 2004 September; 136(1): 2512-2522 |
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Protocols for BioSource 1 |
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Slide: Wang_2-12_Wild-type-Col-5-mM-KNO3-2-hr-roots_Rep2_ATH1 | | |
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Tissue:
| Roots |
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Diseased:
| Treatment |
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in vivo Treatment:
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Treatment | 5 mM KNO3 |
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Additional Organism Information:
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Sample description | Wild type Col, 5 mM KNO3, 2 hr, roots, replicate 2 |
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Other Information:
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Reference | Plant Physiol. 2004 September; 136(1): 2512-2522 |
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Protocols for BioSource 1 |
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Slide: Wang_2-10_Wild-type-Col-5-mM-KNO3-2-hr-roots_Rep1_ATH1 | | |
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Tissue:
| Roots |
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Diseased:
| Treatment |
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in vivo Treatment:
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Treatment | 5 mM KNO3 |
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Additional Organism Information:
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Sample description | Wild type Col, 5 mM KNO3, 2 hr, roots, replicate 1 |
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Other Information:
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Reference | Plant Physiol. 2004 September; 136(1): 2512-2522 |
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Protocols for BioSource 1 |
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Slide: Wang_2-9_Wild-type-Col-5-mM-KCL-2-hr-roots_Rep1_ATH1 | | |
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Tissue:
| Roots |
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Diseased:
| Control |
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in vivo Treatment:
| |
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Treatment | 5 mM KCl |
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Additional Organism Information:
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Sample description | Wild type Col, 5 mM KCL, 2 hr, roots, replicate 1 |
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Other Information:
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Reference | Plant Physiol. 2004 September; 136(1): 2512-2522 |
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Protocols for BioSource 1 |
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Slide: Wang_2-8_Nitrate-reductase-null-mutant-5-mM-KNO3-2-hr-shoots_Rep2_ATH1 | | |
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Tissue:
| shoots |
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Diseased:
| Treatment |
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in vivo Treatment:
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Treatment | 5 mM KNO3 |
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Additional Organism Information:
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Sample description | Nitrate reductase null mutant, 5 mM KNO3, 2 hr, shoots, replicate 2 |
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Other Information:
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Reference | Plant Physiol. 2004 September; 136(1): 2512-2522 |
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Protocols for BioSource 1 |
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Slide: Wang_2-7_Nitrate-reductase-null-mutant-5-mM-KCL-2-hr-shoots_Rep2_ATH1 | | |
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Tissue:
| shoots |
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Diseased:
| Control |
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in vivo Treatment:
| |
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Treatment | 5 mM KCl |
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Additional Organism Information:
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Sample description | Nitrate reductase null mutant, 5 mM KCL, 2 hr, shoots, replicate 2 |
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Other Information:
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Reference | Plant Physiol. 2004 September; 136(1): 2512-2522 |
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Protocols for BioSource 1 |
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Slide: Wang_2-6_Nitrate-reductase-null-mutant-5-mM-KNO3-2-hr-shoots_Rep1_ATH1 | | |
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Tissue:
| shoots |
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Diseased:
| Treatment |
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in vivo Treatment:
| |
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Treatment | 5 mM KNO3 |
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Additional Organism Information:
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Sample description | Nitrate reductase null mutant, 5 mM KNO3, 2 hr, shoots, replicate 1 |
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Other Information:
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Reference | Plant Physiol. 2004 September; 136(1): 2512-2522 |
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Protocols for BioSource 1 |
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Slide: Wang_2-5_Nitrate-reductase-null-mutant-5-mM-KCL-2-hr-shoots_Rep1_ATH1 | | |
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Tissue:
| shoots |
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Diseased:
| Control |
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in vivo Treatment:
| |
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Treatment | 5 mM KCl |
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Additional Organism Information:
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Sample description | Nitrate reductase null mutant, 5 mM KCL, 2 hr, shoots, replicate 1 |
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Other Information:
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Reference | Plant Physiol. 2004 September; 136(1): 2512-2522 |
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Protocols for BioSource 1 |
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Slide: Wang_2-1_Nitrate-reductase-null-mutant-5-mM-KCL-2-hr-roots_Rep1_ATH1 | | |
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Tissue:
| Roots |
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Diseased:
| Control |
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in vivo Treatment:
| |
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Treatment | 5 mM KCl |
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Additional Organism Information:
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Sample description | Nitrate reductase null mutant, 5 mM KCL, 2 hr, roots, replicate 1 |
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Other Information:
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Reference | Plant Physiol. 2004 September; 136(1): 2512-2522 |
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Protocols for BioSource 1 |
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Slide: Wang_2-2_Nitrate-reductase-null-mutant-5-mM-KNO3-2-hr-roots_Rep1_ATH1 | | |
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Tissue:
| Roots |
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Diseased:
| Treatment |
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in vivo Treatment:
| |
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Treatment | 5 mM KNO3 |
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Additional Organism Information:
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Sample description | Nitrate reductase null mutant, 5 mM KNO3, 2 hr, roots, replicate 1 |
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Other Information:
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Reference | Plant Physiol. 2004 September; 136(1): 2512-2522 |
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Protocols for BioSource 1 |
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Slide: Wang_2-3_Nitrate-reductase-null-mutant-5-mM-KCL-2-hr-roots_Rep2_ATH1 | | |
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Tissue:
| Roots |
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Diseased:
| Control |
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in vivo Treatment:
| |
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Treatment | 5 mM KCl |
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Additional Organism Information:
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Sample description | Nitrate reductase null mutant, 5 mM KCL, 2 hr, roots, replicate 2 |
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Other Information:
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Reference | Plant Physiol. 2004 September; 136(1): 2512-2522 |
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Protocols for BioSource 1 |
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Slide: Wang_2-4_Nitrate-reductase-null-mutant-5-mM-KNO3-2-hr-roots_Rep2_ATH1 | | |
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Tissue:
| Roots |
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Diseased:
| Treatment |
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in vivo Treatment:
| |
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Treatment | 5 mM KNO3 |
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Additional Organism Information:
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Sample description | Nitrate reductase null mutant, 5 mM KNO3, 2 hr, roots, replicate 2 |
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Other Information:
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Reference | Plant Physiol. 2004 September; 136(1): 2512-2522 |
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Protocols for BioSource 1 |
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Problems? Comments? Suggestions? Contact the Affymetrix Team