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Experiment: Escherichia coli O157:H7 Induces Stronger Plant Immunity than Salmonella enterica Typhimurium SL1344
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-674
There have been increased concerns of leafy vegetable contamination by human pathogens and food safety due to outbreaks associated with consumption of fresh produce. In this study, we assessed defense responses induced by the fully pathogenic bacteria Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium SL1344 in both Arabidopsis thaliana. Unlike SL1344, O157:H7 induces strong plant immunity at both pre-invasion and post-invasion steps of infection. For instance, O157:H7 triggers stomatal closure even under high relative humidity (RH); an environmental condition that generally weakens plant defenses against bacteria in the field and laboratory conditions. SL1344 instead induces a transient stomatal immunity. We also observed that O157:H7 modulates more components of innate immunity, i.e. genes and signaling pathways, as compared to SL1344. These results suggest that plants may recognize and respond to some human pathogens more effectively than others. Furthermore, stomatal immunity can diminish the penetration of human pathogens through the leaf epidermis resulting in low bacterial titers in the plant apoplast, which suggest that additional control measures can be employed to prevent food contamination. The understanding of how plant responses can diminish bacterial contamination is paramount in preventing outbreaks and improving the safety of food's supplies.
About the ExperimenterName: | Shweta Panchal |
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Head of Lab Name: | Dr. Maeli Melotto |
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Lab:
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Address: | 501 S. Nedderman Dr Box 19498 Arlington, TX
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Postcode:
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Country:
| USA |
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work based on any of this data, please acknowledge us, contact the
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| About this ExperimentExperiment Type:
| infect |
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Number of Slides: | 6 |
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| Experimental Parameters:
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parameter | infect |
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Quality Control Measures Taken:
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References:
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Slides in this Experiment
Hybridisation Set: Panchal: Escherichia coli O157:H7 Induces Stronger Plant Immunity than Salmonella enterica Typhimurium SL1344
Slide: Panchal_674-6_Mock_7h_Rep3_ATH1 | | |
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Protocols for BioSource 1 |
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Slide: Panchal_674-5_Mock_7h_Rep2_ATH1 | | |
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Protocols for BioSource 1 |
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Slide: Panchal_674-4_Mock_7h_Rep1_ATH1 | | |
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Protocols for BioSource 1 |
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Slide: Panchal_674-3_0e7-SL1344_7h_Rep3_ATH1 | | |
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Protocols for BioSource 1 |
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Slide: Panchal_674-1_0e7-SL1344_7h_Rep1_ATH1 | | |
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Protocols for BioSource 1 |
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Slide: Panchal_674-2_0e7-SL1344_7h_Rep2_ATH1 | | |
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Protocols for BioSource 1 |
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