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Experiment: ATHB1 mutant response to methyl jasmonate
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-572
ATHB1 is a member of the HD-ZIP I family of transcription factors, peculiar to plants. To understand the functional role of ATHB-1 in Arabidopsis, we identified athb1-1 and athb1-2 loss-of-function insertional mutants. Both alleles do not show any phenotypic alteration respect to overall size and morphology under our growth conditions, likely due to a functional redundancy within the HD-ZIP I family. However, gene expression analysis suggests that ATHB1 could take part in plant responses to environmental stresses. In fact, ATHB1 expression is affected by flooding and wounding, and by ethylene and methyl jasmonate (MeJA). Moreover, although wild type and mutant plants were clearly impaired by MeJA in a dose dependent manner, both athb1 alleles were less affected and showed a reduced rate of seedling mortality.In order to investigate the role of ATHB-1 in the MeJA response at genomic level, a transcriptome analysis will be performed on wild type (Ler) and athb1-2 plants treated with 30 μM MeJA. To this end, RNA samples have been prepared from 1 week-old plants treated with MeJA or ethanol (mock) for 1 hour and 4 hour.
About the ExperimenterName: | Simona Baima |
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Head of Lab Name: | Giorgio Morelli |
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Lab:
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Address: | Istituto Nazionale di Ricerca per gli Alimenti e la Nutrizione (INRAN) Via Ardeatina 546, Rome, Roma, , 00178, ITALY
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Postcode:
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Country:
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| Telephone Number:
| +39-06-51494453 |
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Fax Number:
| +39-06-51494550 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| compound_based_treatment |
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Number of Slides: | 24 |
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| Experimental Parameters:
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parameter | compound_based_treatment |
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Quality Control Measures Taken:
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Baima: athb1 mutant response to methyl jasmonate
Slide: Baima_572-1_Ler_EtOH_1h_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction. |
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Additional Organism Information:
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Sample Description | Plants didnt show any alteration after the treatment. |
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Other Information:
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Timecourse start procedure | Start time was the time when 7-days-old seedlings were transferred on MS medium containing MeJA or ethanol. |
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Protocols for BioSource 1 |
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Slide: Baima_572-2_athb1-2_EtOH_1h_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction.
This sample is the control: mock treatment for 1h (This is the first of three experimental replicates). |
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Additional Organism Information:
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Sample Description | Plants didnt show any alteration after the treatment. |
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Other Information:
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Timecourse start procedure | Start time was the time when 7-days-old seedlings were transferred on MS medium containing MeJA or ethanol. |
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Protocols for BioSource 1 |
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Slide: Baima_572-3_Ler_MeJA_1h_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction.
This sample has been treated with 30 μM MeJA for 1h (This is the first of three experimental replicates). |
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Additional Organism Information:
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Sample Description | Plants didnt show any alteration after the treatment. |
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Other Information:
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Timecourse start procedure | Start time was the time when 7-days-old seedlings were transferred on MS medium containing MeJA or ethanol. |
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Protocols for BioSource 1 |
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Slide: Baima_572-4_athb1-2_MeJA_1h_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction.
This sample has been treated with 30 μM MeJA for 1h (This is the first of three experimental replicates). |
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Additional Organism Information:
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Sample Description | Plants didnt show any alteration after the treatment. |
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Other Information:
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Timecourse start procedure | Start time was the time when 7-days-old seedlings were transferred on MS medium containing MeJA or ethanol. |
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Protocols for BioSource 1 |
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Slide: Baima_572-5_Ler_EtOH_4h_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction.
This sample is the control: mock treatment for 4h (This is the first of three experimental replicates). |
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Additional Organism Information:
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Sample Description | Plants didnt show any alteration after the treatment. |
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Other Information:
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Timecourse start procedure | Start time was the time when 7-days-old seedlings were transferred on MS medium containing MeJA or ethanol. |
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Protocols for BioSource 1 |
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Slide: Baima_572-6_athb1-2_EtOH_4h_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction.
This sample is the control: mock treatment for 4h (This is the first of three experimental replicates). |
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Additional Organism Information:
| |
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Sample Description | Plants didnt show any alteration after the treatment. |
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Other Information:
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Timecourse start procedure | Start time was the time when 7-days-old seedlings were transferred on MS medium containing MeJA or ethanol. |
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Protocols for BioSource 1 |
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Slide: Baima_572-7_Ler_MeJA_4h_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction.
This sample has been treated with 30 μM MeJA for 4h (This is the first of three experimental replicates). |
---|
Additional Organism Information:
| |
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Sample Description | Plants didnt show any alteration after the treatment. |
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Other Information:
| |
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Timecourse start procedure | Start time was the time when 7-days-old seedlings were transferred on MS medium containing MeJA or ethanol. |
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Protocols for BioSource 1 |
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Slide: Baima_572-8_athb1-2_MeJA_4h_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction.
This sample has been treated with 30 μM MeJA for 4h (This is the first of three experimental replicates). |
---|
Additional Organism Information:
| |
---|
Sample Description | Plants didnt show any alteration after the treatment. |
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Other Information:
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Timecourse start procedure | Start time was the time when 7-days-old seedlings were transferred on MS medium containing MeJA or ethanol. |
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Protocols for BioSource 1 |
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Slide: Baima_572-9_Ler_EtOH_1h_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction.
This sample is the control: mock treatment for 1h (This is the second of three experimental replicates). |
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Additional Organism Information:
| |
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Sample Description | Plants didnt show any alteration after the treatment. |
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Other Information:
| |
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Timecourse start procedure | Start time was the time when 7-days-old seedlings were transferred on MS medium containing MeJA or ethanol. |
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Protocols for BioSource 1 |
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Slide: Baima_572-10_athb1-2_EtOH_1h_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction.
This sample is the control: mock treatment for 1h (This is the second of three experimental replicates). |
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Additional Organism Information:
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Sample Description | Plants didnt show any alteration after the treatment. |
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Other Information:
| |
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Timecourse start procedure | Start time was the time when 7-days-old seedlings were transferred on MS medium containing MeJA or ethanol. |
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Protocols for BioSource 1 |
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Slide: Baima_572-11_Ler_MeJA_1h_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction.
This sample has been treated with 30 μM MeJA for 1h (This is the second of three experimental replicates). |
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Additional Organism Information:
| |
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Sample Description | Plants didnt show any alteration after the treatment. |
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Other Information:
| |
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Timecourse start procedure | Start time was the time when 7-days-old seedlings were transferred on MS medium containing MeJA or ethanol. |
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Protocols for BioSource 1 |
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Slide: Baima_572-12_athb1-2_MeJA_1h_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction.
This sample has been treated with 30 μ;M MeJA for 1h (This is the second of three experimental replicates). |
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Additional Organism Information:
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Sample Description | Plants didnt show any alteration after the treatment. |
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Other Information:
| |
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Timecourse start procedure | Start time was the time when 7-days-old seedlings were transferred on MS medium containing MeJA or ethanol. |
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Protocols for BioSource 1 |
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Slide: Baima_572-13_Ler_EtOH_4h_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction.
This sample is the control: mock treatment for 4h (This is the second of three experimental replicates). |
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Additional Organism Information:
| |
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Sample Description | Plants didnt show any alteration after the treatment. |
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Other Information:
| |
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Timecourse start procedure | Start time was the time when 7-days-old seedlings were transferred on MS medium containing MeJA or ethanol. |
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Protocols for BioSource 1 |
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Slide: Baima_572-14_athb1-2_EtOH_4h_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction.
This sample is the control: mock treatment for 4h (This is the second of three experimental replicates). |
---|
Additional Organism Information:
| |
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Sample Description | Plants didnt show any alteration after the treatment. |
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Other Information:
| |
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Timecourse start procedure | Start time was the time when 7-days-old seedlings were transferred on MS medium containing MeJA or ethanol. |
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Protocols for BioSource 1 |
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Slide: Baima_572-15_Ler_MeJA_4h_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction.
This sample has been treated with 30 μM MeJA for 4h (This is the second of three experimental replicates). |
---|
Additional Organism Information:
| |
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Sample Description | Plants didnt show any alteration after the treatment. |
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Other Information:
| |
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Timecourse start procedure | Start time was the time when 7-days-old seedlings were transferred on MS medium containing MeJA or ethanol. |
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Protocols for BioSource 1 |
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Slide: Baima_572-16_athb1-2_MeJA_4h_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction.
This sample has been treated with 30 μM MeJA for 4h (This is the second of three experimental replicates). |
---|
Additional Organism Information:
| |
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Sample Description | Plants didnt show any alteration after the treatment. |
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Other Information:
| |
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Timecourse start procedure | Start time was the time when 7-days-old seedlings were transferred on MS medium containing MeJA or ethanol. |
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Protocols for BioSource 1 |
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Slide: Baima_572-17_Ler_EtOH_1h_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction.
This sample is the control: mock treatment for 1h (This is the third of three experimental replicates). |
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Additional Organism Information:
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Sample Description | Plants didnt show any alteration after the treatment. |
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Other Information:
| |
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Timecourse start procedure | Start time was the time when 7-days-old seedlings were transferred on MS medium containing MeJA or ethanol. |
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Protocols for BioSource 1 |
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Slide: Baima_572-18_athb1-2_EtOH_1h_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction.
This sample is the control: mock treatment for 1h (This is the third of three experimental replicates). |
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Additional Organism Information:
| |
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Sample Description | Plants didnt show any alteration after the treatment. |
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Other Information:
| |
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Timecourse start procedure | Start time was the time when 7-days-old seedlings were transferred on MS medium containing MeJA or ethanol. |
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Protocols for BioSource 1 |
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Slide: Baima_572-19_Ler_MeJA_1h_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction.
This sample has been treated with 30 μM MeJA for 1h (This is the third of three experimental replicates). |
---|
Additional Organism Information:
| |
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Sample Description | Plants didnt show any alteration after the treatment. |
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Other Information:
| |
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Timecourse start procedure | Start time was the time when 7-days-old seedlings were transferred on MS medium containing MeJA or ethanol. |
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Protocols for BioSource 1 |
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Slide: Baima_572-20_athb1-2_MeJA_1h_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction.
This sample has been treated with 30 μM MeJA for 1h (This is the third of three experimental replicates). |
---|
Additional Organism Information:
| |
---|
Sample Description | Plants didnt show any alteration after the treatment. |
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Other Information:
| |
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Timecourse start procedure | Start time was the time when 7-days-old seedlings were transferred on MS medium containing MeJA or ethanol. |
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|
Protocols for BioSource 1 |
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Slide: Baima_572-21_Ler_EtOH_4h_Rep1_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction.
This sample is the control: mock treatment for 4h (This is the third of three experimental replicates). |
---|
Additional Organism Information:
| |
---|
Sample Description | Plants didnt show any alteration after the treatment. |
---|
Other Information:
| |
---|
Timecourse start procedure | Start time was the time when 7-days-old seedlings were transferred on MS medium containing MeJA or ethanol. |
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|
Protocols for BioSource 1 |
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Slide: Baima_572-22_athb1-2_EtOH_4h_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction.
This sample is the control: mock treatment for 4h (This is the third of three experimental replicates). |
---|
Additional Organism Information:
| |
---|
Sample Description | Plants didnt show any alteration after the treatment. |
---|
Other Information:
| |
---|
Timecourse start procedure | Start time was the time when 7-days-old seedlings were transferred on MS medium containing MeJA or ethanol. |
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|
Protocols for BioSource 1 |
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Slide: Baima_572-23_Ler_MeJA_4h_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction.
This sample has been treated with 30 μM MeJA for 4h (This is the third of three experimental replicates). |
---|
Additional Organism Information:
| |
---|
Sample Description | Plants didnt show any alteration after the treatment. |
---|
Other Information:
| |
---|
Timecourse start procedure | Start time was the time when 7-days-old seedlings were transferred on MS medium containing MeJA or ethanol. |
---|
|
Protocols for BioSource 1 |
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Slide: Baima_572-24_athb1-2_MeJA_4h_Rep3_ATH1 | | |
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Tissue:
| whole plant |
---|
in vivo Treatment:
| Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction.
This sample has been treated with 30 μM MeJA for 4h (This is the third of three experimental replicates). |
---|
Additional Organism Information:
| |
---|
Sample Description | Plants didnt show any alteration after the treatment. |
---|
Other Information:
| |
---|
Timecourse start procedure | Start time was the time when 7-days-old seedlings were transferred on MS medium containing MeJA or ethanol. |
---|
|
Protocols for BioSource 1 |
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Problems? Comments? Suggestions? Contact the Affymetrix Team