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Experiment: Clarification of the genetic basis of the iae1 and iae2 phenotypes
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-96
The mutants iae1 and iae2 have been mapped to two distinct loci on chromosome 2. With the aid of the GetCID service, we believe iae1 has been localised to a relatively small region (~100kb); while mapping has restricted iae2 to a small interval (~50kb) with a very limited number of candidate genes. Although it has proved difficult to determine which gene is mutated in iae1, we belive that use of the transcriptomics service will help identify which genes are affected (directly and indirectly) in this background. The iae2 locus is sufficiently restricted by genetics and by molecular data that identifying a candidate (and any downstream loci affected) should be straightforward. We require the use of the full genome chips. The data from this experiment should help tie together the data we have for a paper that is currently in preparation.
About the ExperimenterName: | Dr David Stevenson |
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Head of Lab Name: | Dr Paul Jarvis |
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Lab:
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Institute:
| University of Leicester |
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Address: | University of Leicester Department of Biology University Road Leicester
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Postcode:
| LE1 7RH |
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Country:
| UK |
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| Telephone Number:
| 0116-2525664 |
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Fax Number:
| 0116-2523330 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| genetic_modification_design |
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Number of Slides: | 9 |
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| Experimental Parameters:
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parameter | genetic_modification |
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Quality Control Measures Taken:
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no-plants-pooled | 30 (done) |
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Stevenson_genome_Rep1
Slide: Stevenson-jarvis_2-4_iae2_Rep1_ATH1 | | |
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Tissue:
| crown, hypocotyl, cotyledons and apical meristem bud |
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Diseased:
| Normal |
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Separation Technique:
| Seedlings were removed from the agar plates on which they were grown by severing the seedling at the crown with scissors |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Stevenson-jarvis_2-7_B1798_Rep1_ATH1 | | |
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Tissue:
| crown, hypocotyl, cotyledons and apical meristem bud |
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Diseased:
| Normal |
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Separation Technique:
| Seedlings were removed from the agar plates on which they were grown by severing the seedling at the crown with scissors |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Stevenson-jarvis_2-1_iae1_Rep1_ATH1 | | |
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Tissue:
| crown, hypocotyl, cotyledons and apical meristem bud |
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Diseased:
| Normal |
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Separation Technique:
| Seedlings were removed from the agar plates on which they were grown by severing the seedling at the crown with scissors |
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Other Information:
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Protocols for BioSource 1 |
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Hybridisation Set: Stevenson_genome_Rep2
Slide: Stevenson-jarvis_2-5_iae2_Rep2_ATH1 | | |
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Tissue:
| crown, hypocotyl, cotyledons and apical meristem bud |
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Diseased:
| Normal |
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Separation Technique:
| Seedlings were removed from the agar plates on which they were grown by severing the seedling at the crown with scissors |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Stevenson-jarvis_2-8_B1798_Rep2_ATH1 | | |
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Tissue:
| crown, hypocotyl, cotyledons and apical meristem bud |
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Diseased:
| Normal |
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Separation Technique:
| Seedlings were removed from the agar plates on which they were grown by severing the seedling at the crown with scissors |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Stevenson-jarvis_2-2_iae1_Rep2_ATH1 | | |
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Tissue:
| crown, hypocotyl, cotyledons and apical meristem bud |
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Diseased:
| Normal |
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Separation Technique:
| Seedlings were removed from the agar plates on which they were grown by severing the seedling at the crown with scissors |
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Other Information:
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Protocols for BioSource 1 |
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Hybridisation Set: Stevenson_genome_Rep3
Slide: Stevenson-jarvis_2-6_iae2_Rep3_ATH1 | | |
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Tissue:
| crown, hypocotyl, cotyledons and apical meristem bud |
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Diseased:
| Normal |
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Separation Technique:
| Seedlings were removed from the agar plates on which they were grown by severing the seedling at the crown with scissors |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Stevenson-jarvis_2-3_iae1_Rep3_ATH1 | | |
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Tissue:
| crown, hypocotyl, cotyledons and apical meristem bud |
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Diseased:
| Normal |
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Separation Technique:
| Seedlings were removed from the agar plates on which they were grown by severing the seedling at the crown with scissors |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Stevenson-jarvis_2-9_B1798_Rep3_ATH1 | | |
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Tissue:
| crown, hypocotyl, cotyledons and apical meristem bud |
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Diseased:
| Normal |
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Separation Technique:
| Seedlings were removed from the agar plates on which they were grown by severing the seedling at the crown with scissors |
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Other Information:
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Protocols for BioSource 1 |
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Problems? Comments? Suggestions? Contact the Affymetrix Team