 | NASCArrays | ,
,
,
,
,
,
,
,
,
,
|
Experiment: Response of the transcription factor mutants wrky11and wrky17 to virulent Pseudomonas(Pst DC3000)
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-393
WRKY11 and WRKY17 act in a partially redundant manner as negative regulators of basal resistance to Pseudomonas. In order to identify candidate target genes that are either directly or indirectly regulated by them, a genome-wide expression analysis in mutant plants was undertaken.Three independent experiments were performed in which leaves of 4 week-old wild-type Col-0, wrky11.1 (ZIGIA En-insertion line 5AAH57) or wrky17.1 plants (T-DNA insertion lines SALK_176337) were inoculated with Pst DC3000 (107 cfu per mL) and harvested 0, 2, 5 and 12 hours after inoculation. After verifying the induction of marker genes of the defence response by Q-RT-PCR, each individual sample from each of the three biologically independent experiments was used for the hybridisation of one GeneChip.
About the ExperimenterName: | Dr Thomas KROJ |
---|
Head of Lab Name: | Dr Dominique ROBY |
---|
Lab:
| |
---|
Institute:
| UMR CNRS-INRA |
---|
Address: | LIPM UMR CNRS-INRA 2594 Chemin de Borderouge BP 52627 CASTANET-TOLOSAN cedex
|
---|
Postcode:
| 31326 |
---|
Country:
| FRANCE |
---|
| Telephone Number:
| 33 5 61 28 53 26 |
---|
Fax Number:
| 33 5 61 28 50 61 |
---|
All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
|
| About this ExperimentExperiment Type:
| genetic_modification_design; time_series_design;compound_treatment_design |
---|
Number of Slides: | 36 |
---|
| Experimental Parameters:
|
|
---|
Parameter | compound_based_treatment |
---|
parameter | gene_knock_out |
---|
parameter | timepoint |
---|
Quality Control Measures Taken:
| |
---|
References:
| |
---|
| Other Information:
| |
---|
|
Slides in this Experiment
Hybridisation Set: KROJ: Response to wrky11and wrky17 to virulent Pst DC3000_genome
Slide: Kroj_1-1_Col-0-0h_Rep1_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of 107 cfu per mL using a blunt syringe on the abaxial side of the leaves. (for whole leaves) |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-2_Col-0-2h_Rep1_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of 107 cfu per mL using a blunt syringe on the abaxial side of the leaves. (for whole leaves) |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-3_Col-0-5h_Rep1_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of 107 cfu per mL using a blunt syringe on the abaxial side of the leaves. (for whole leaves) |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-4_Col-0-12h_Rep1_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of 107 cfu per mL using a blunt syringe on the abaxial side of the leaves. (for whole leaves) |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-5_wrky11-0h_Rep1_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of Pst DC3000 at 107 using a blunt syringe on the abaxial side of the leaves. 4 leaves per plant were inoculated |
---|
Additional Organism Information:
| ZIGIA EN insertion line 5AAH57 |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-6_wrky11-2h_Rep1_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of Pst DC3000 at 107 using a blunt syringe on the abaxial side of the leaves. 4 leaves per plant were inoculated |
---|
Additional Organism Information:
| ZIGIA EN insertion line 5AAH57 |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-7_wrky11-5h_Rep1_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of Pst DC3000 at 107 using a blunt syringe on the abaxial side of the leaves. 4 leaves per plant were inoculated |
---|
Additional Organism Information:
| ZIGIA EN insertion line 5AAH57 |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-8_wrky11-12h_Rep1_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of Pst DC3000 at 107 using a blunt syringe on the abaxial side of the leaves. 4 leaves per plant were inoculated |
---|
Additional Organism Information:
| ZIGIA EN insertion line 5AAH57 |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-9_wrky17-0h_Rep1_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of Pst DC3000 at 107 using a blunt syringe on the abaxial side of the leaves. 4 leaves per plant were inoculated |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-10_wrky17-2h_Rep1_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of Pst DC3000 at 107 using a blunt syringe on the abaxial side of the leaves. 4 leaves per plant were inoculated |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-11_wrky17-5h_Rep1_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of Pst DC3000 at 107 using a blunt syringe on the abaxial side of the leaves. 4 leaves per plant were inoculated |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-12_wrky17-12h_Rep1_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of Pst DC3000 at 107 using a blunt syringe on the abaxial side of the leaves. 4 leaves per plant were inoculated |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-13_Col-0-0h_Rep2_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of 107 cfu per mL using a blunt syringe on the abaxial side of the leaves. (for whole leaves) |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-14_Col-0-2h_Rep2_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of 107 cfu per mL using a blunt syringe on the abaxial side of the leaves. (for whole leaves) |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-15_Col-0-5h_Rep2_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of 107 cfu per mL using a blunt syringe on the abaxial side of the leaves. (for whole leaves) |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-16_Col-0-12h_Rep2_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of 107 cfu per mL using a blunt syringe on the abaxial side of the leaves. (for whole leaves) |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-17_wrky11-0h_Rep2_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of Pst DC3000 at 107 using a blunt syringe on the abaxial side of the leaves. 4 leaves per plant were inoculated |
---|
Additional Organism Information:
| ZIGIA EN insertion line 5AAH57 |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-18_wrky11-2h_Rep2_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of Pst DC3000 at 107 using a blunt syringe on the abaxial side of the leaves. 4 leaves per plant were inoculated |
---|
Additional Organism Information:
| ZIGIA EN insertion line 5AAH57 |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-19_wrky11-5h_Rep2_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of Pst DC3000 at 107 using a blunt syringe on the abaxial side of the leaves. 4 leaves per plant were inoculated |
---|
Additional Organism Information:
| ZIGIA EN insertion line 5AAH57 |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-20_wrky11-12h_Rep2_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of Pst DC3000 at 107 using a blunt syringe on the abaxial side of the leaves. 4 leaves per plant were inoculated |
---|
Additional Organism Information:
| ZIGIA EN insertion line 5AAH57 |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-21_wrky17-0h_Rep2_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of Pst DC3000 at 107 using a blunt syringe on the abaxial side of the leaves. 4 leaves per plant were inoculated |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-22_wrky17-2h_Rep2_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of Pst DC3000 at 107 using a blunt syringe on the abaxial side of the leaves. 4 leaves per plant were inoculated |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-23_wrky17-5h_Rep2_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of Pst DC3000 at 107 using a blunt syringe on the abaxial side of the leaves. 4 leaves per plant were inoculated |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-24_wrky17-12h_Rep2_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of Pst DC3000 at 107 using a blunt syringe on the abaxial side of the leaves. 4 leaves per plant were inoculated |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-25_Col-0-0h_Rep3_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of 107 cfu per mL using a blunt syringe on the abaxial side of the leaves. (for whole leaves) |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-26_Col-0-2h_Rep3_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of 107 cfu per mL using a blunt syringe on the abaxial side of the leaves. (for whole leaves) |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-27_Col-0-5h_Rep3_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of 107 cfu per mL using a blunt syringe on the abaxial side of the leaves. (for whole leaves) |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-28_Col-0-12h_Rep3_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of 107 cfu per mL using a blunt syringe on the abaxial side of the leaves. (for whole leaves) |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-29_wrky11-0h_Rep3_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of Pst DC3000 at 107 using a blunt syringe on the abaxial side of the leaves. 4 leaves per plant were inoculated |
---|
Additional Organism Information:
| ZIGIA EN insertion line 5AAH57 |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-30_wrky11-2h_Rep3_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of Pst DC3000 at 107 using a blunt syringe on the abaxial side of the leaves. 4 leaves per plant were inoculated |
---|
Additional Organism Information:
| ZIGIA EN insertion line 5AAH57 |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-31_wrky11-5h_Rep3_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of Pst DC3000 at 107 using a blunt syringe on the abaxial side of the leaves. 4 leaves per plant were inoculated |
---|
Additional Organism Information:
| ZIGIA EN insertion line 5AAH57 |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-32_wrky11-12h_Rep3_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of Pst DC3000 at 107 using a blunt syringe on the abaxial side of the leaves. 4 leaves per plant were inoculated |
---|
Additional Organism Information:
| ZIGIA EN insertion line 5AAH57 |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-33_wrky17-0h_Rep3_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of Pst DC3000 at 107 using a blunt syringe on the abaxial side of the leaves. 4 leaves per plant were inoculated |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-34_wrky17-2h_Rep3_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of Pst DC3000 at 107 using a blunt syringe on the abaxial side of the leaves. 4 leaves per plant were inoculated |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-35_wrky17-5h_Rep3_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of Pst DC3000 at 107 using a blunt syringe on the abaxial side of the leaves. 4 leaves per plant were inoculated |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Kroj_1-36_wrky17-12h_Rep3_ATH1 | | |
|
|
|
Tissue:
| leaves |
---|
in vivo Treatment:
| |
---|
treatment protocol | Plants used for bacterial inoculations were kept at high humidity 12 h before inoculation. They were injected with a bacterial suspension of Pst DC3000 at 107 using a blunt syringe on the abaxial side of the leaves. 4 leaves per plant were inoculated |
---|
Other Information:
| |
---|
|
Protocols for BioSource 1 |
|
|
|
Problems? Comments? Suggestions? Contact the Affymetrix Team