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Experiment: Cell type specific analysis of root epidermis
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-502
The experiment aims to analyse auxin signalling in root epidermis. We used two GFP marker lines (pCOBL9:GFP, pGL2:GFP) and fluorescent activated cell sorting to generate samples of trichoblast and atrichobast cells. Before protoplasting the roots were either mock treated or exposed to IAA (10µM) for 2 hours. Eventually the study will establish the topology of the auxin response system in these cell types and together with data from other sources will enable us to develop a model of auxin signalling in root epidermis. These samples represent a scaled down experiment aimed at testing the efficiency of our experimental protocol from cell sorting to microarray analysis.
About the ExperimenterName: | DR martin Kieffer |
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Head of Lab Name: | DR Stefan Kepinski |
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Lab:
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Address: | Centre for plant sciences University of Leeds LEEDS
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Postcode:
| LS29JT |
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Country:
| UK |
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| About this ExperimentExperiment Type:
| compound_treatment_design; |
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Number of Slides: | 7 |
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| Experimental Parameters:
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Quality Control Measures Taken:
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References:
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| Other Information:
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title | Cell type specific analysis of root epidermis |
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Slides in this Experiment
Hybridisation Set: Kieffer: Cell type specific analysis of root epidermis_genome
Slide: Kieffer_1-2_Atricoblast-mock_Rep2_ATH1 | | |
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Tissue:
| root tips |
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Additional Organism Information:
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Sample Description | Roots from tissue cultured plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Kieffer_1-1_Atricoblast-mock_Rep1_ATH1 | | |
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Tissue:
| root tips |
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Additional Organism Information:
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Sample Description | Roots from tissue cultured plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Kieffer_1-3_Atricoblast-auxin_Rep1_ATH1 | | |
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Tissue:
| root tips |
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in vivo Treatment:
| IAA 1OµM for 2hrs |
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Additional Organism Information:
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Sample Description | Roots from tissue cultured plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Kieffer_1-4_Atricoblast-auxin_Rep2_ATH1 | | |
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Tissue:
| root tips |
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in vivo Treatment:
| IAA 1OµM for 2hrs |
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Additional Organism Information:
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Sample Description | Roots from tissue cultured plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Kieffer_1-5_Trichoblast-mock_Rep1_ATH1 | | |
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Tissue:
| root tips |
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Additional Organism Information:
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Sample Description | Roots from tissue cultured plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Kieffer_1-6_Trichoblast-mock_Rep2_ATH1 | | |
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Tissue:
| root tips |
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Additional Organism Information:
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Sample Description | Roots from tissue cultured plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Kieffer_1-7_Trichoblast-auxin_Rep1_ATH1 | | |
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Tissue:
| root tips |
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in vivo Treatment:
| IAA 1OµM for 2hrs |
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Additional Organism Information:
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Sample Description | Roots from tissue cultured plants |
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Other Information:
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Protocols for BioSource 1 |
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