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Experiment: Seed development
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-399
Reciprocal crosses of a diploid Arabidopsis with different ploidies results in different endosperm development patterns and phenotype. Typically if the ploidy is higher on the maternal side there are fewer endosperm cells which cellularize early and conversely if the paternal ploidy is higher, there is more number of endosperm cells which cellularize late. Crosses involving diploid and tetraploid Arabidopsis (C24) are viable whereas crosses involving the diploid and hexaploid even though exhibit the above mentioned directional trend in endosperm development, abort (Scott et al 1998). The 'maternalised' and 'paternalised' development of endosperm is also observed in crosses involving some Arabidopsis mutants. Mutants in the fis class of genes e.g. fis1-medea when crossed with a diploid Arabidopsis (pollen parent) show endosperm development-seed development similar to a diploid (seed parent) crossed with hexaploid pollen parent. Reciprocal crosses of homozygous met1 and diploid Arabidopsis also exhibit reciprocal trends in endosperm development where a homozygous met1 mutant (seed parent) crossed with diploid is similar in phenotype to a diploid (seed parent) - tetraploid cross. Endosperm development in the reciprocal cross has phenotypic similarity to the tetraploid (seed parent)-diploid cross (Adams et al 2000).We are interested in understanding gene profiles and trends in expression underlying the endosperm development in the interploidy crosses as well as the fis and met1 mutant.
About the ExperimenterName: | Dr Sushma Tiwari |
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Head of Lab Name: | Prof Rod Scott |
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Lab:
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Institute:
| University of Bath |
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Address: | 1.13, 3 South, Biology and Biochemistry University of Bath Claverton down Bath
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Postcode:
| BA2 7AY |
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Country:
| UK |
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| Telephone Number:
| 01225385427 |
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are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
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co-authors in the work.
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| About this ExperimentExperiment Type:
| strain_or_line_design; genetic_modification_design |
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Number of Slides: | 11 |
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| Experimental Parameters:
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parameter | strain_or_line |
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parameter | gene_knock_out |
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Quality Control Measures Taken:
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References:
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Slides in this Experiment
Hybridisation Set: Tiwari: Seed development_genome
Slide: Tiwari_2-7_2x-X-2x-5dap_Rep1_ATH1 | | |
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Tissue:
| siliques 5 days after fertilization |
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Additional Organism Information:
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Sample Description | |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Tiwari_2-6_fis1-X-2x-2-5dap_Rep2_ATH1 | | |
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Tissue:
| siliques 5 days after fertilization |
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Additional Organism Information:
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Sample Description | |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Tiwari_2-5_fis1-X-2x-1-5dap_Rep1_ATH1 | | |
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Tissue:
| siliques 5 days after fertilization |
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Additional Organism Information:
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Sample Description | |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Tiwari_2-4_2x-X-met1-2-5dap_Rep2_ATH1 | | |
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Tissue:
| siliques 5 days after fertilization |
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Additional Organism Information:
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Sample Description | |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Tiwari_2-3_2x-X-met1-1-5dap_Rep1_ATH1 | | |
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Tissue:
| siliques 5 days after fertilization |
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Additional Organism Information:
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Sample Description | |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Tiwari_2-2_met1-X-2x-2-5dap_Rep2_ATH1 | | |
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Tissue:
| siliques 5 days after fertilization |
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Additional Organism Information:
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Sample Description | |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Tiwari_2-1_met1-X-2x-1-5dap_Rep1_ATH1 | | |
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Tissue:
| siliques 5 days after fertilization |
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Additional Organism Information:
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Sample Description | |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Tiwari_2-8_2x-X-4x-5dap_Rep1_ATH1 | | |
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Tissue:
| siliques 5 days after fertilization |
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Additional Organism Information:
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Sample Description | seed parent crossed with pollen parent as interploidies indicated in the sample name |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Tiwari_2-9_4x-X-2x-5dap_Rep1_ATH1 | | |
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Tissue:
| siliques 5 days after fertilization |
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Additional Organism Information:
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Sample Description | seed parent crossed with pollen parent as interploidies indicated in the sample name |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Tiwari_2-10_2x-X-6x-5dap_Rep1_ATH1 | | |
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Tissue:
| siliques 5 days after fertilization |
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Additional Organism Information:
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Sample Description | seed parent crossed with pollen parent as interploidies indicated in the sample name |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Tiwari_2-11_6x-X-2x-5dap_Rep1_ATH1 | | |
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Tissue:
| siliques 5 days after fertilization |
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Additional Organism Information:
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Sample Description | seed parent crossed with pollen parent as interploidies indicated in the sample name |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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