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Experiment: Investigating the molecular response of Arabidopsis to combined nematode and dehydration stress using the Affymetrix ATH1 microarray.
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-489
Plants respond to stress by activating differential signalling pathways leading to tolerance and resistance. Response to individual abiotic and biotic stresses has been well studied. There are key elements in common between the two pathways including the interplay of plant hormones, transcription factors and signal transduction cascades. It is thought that the two pathways may also act antagonistically in order to prioritise stress response, a process governed by the hormones abscisic acid and jasmonic acid (Anderson et al., 2004 Plant Cell 16: 3460-3479). Little is known about the molecular effect of simultaneous abiotic and biotic stress in plants. This study will characterise transcriptome changes in response to combined abiotic and biotic stress, namely dehydration and nematode infection. The response to these stresses will be analysed in roots and leaves. Arabidopsis Col-0 plants were grown on half MS media for 18 days (growth stage 1.08-1.12, Boyes et al., 2001 Plant Cell 13:1499-1510). Half the plants were challenged with rigorously sterilised, infective Heterodera schachtii nematodes as described before (Fuller et al., 2007 Molecular Plant Pathology 8: 595-609). Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points. Control plants were mock inoculated with sterile water. At 10 days post infection (growth stage 3.2-3.5, Boyes et al, 2001) half the plants from each group were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar in the growth cabinet for a further 30 minutes before harvesting the tissue. Control plants were lifted off the agar and then immediately pla ced back in the growth cabinet for 45 minutes. Root and leaf tissue was harvested separately from each of the four treatment groups and RNA isolated using an RNeasy plant RNA preparation kit (Qiagen). The experiment will be carried out three times to provide biological replicates.
About the ExperimenterName: | Miss Nicola Spencer-Jones |
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Head of Lab Name: | Dr. Peter Urwin |
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Lab:
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Address: | 9.91c Manton Centre for Plant Sciences Faculty of Biological Sciences University of Leeds Woodhouse Lane
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Postcode:
| LS2 9JT |
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Country:
| UK |
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| Telephone Number:
| 01133433035 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| pathogenicity_design |
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Number of Slides: | 24 |
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| Experimental Parameters:
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parameter | infect |
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Quality Control Measures Taken:
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Spencer-Jones: Investigating the molecular response of Arabidopsis to combined nematode and dehydration stress using the Affymetrix ATH1 microarray._genome
Slide: Spencer-Jones_1-24_H.schachtii-dehydrated-root_Rep3_ATH1 | | |
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Tissue:
| roots |
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in vivo Treatment:
| At growth stage 1.081.12 (Boyes et al., 2001) plants were randomly allocated into control and treatment groups. Roots of these treatment plants were infected with H. schachtii J2 stage nematodes. Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points. Control plants were mock inoculated with sterile water.
10 days later plants the plants were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar and put back in the growth cabinet for a further 30 minutes before harvesting the tissue. |
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Additional Organism Information:
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Sample Description | Following dehydration treatment and 30 minutes back in the growth cabinet, root material was separated from the green parts of the plant and was immediately frozen in liquid nitrogen. RNA was pooled from 40 plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Spencer-Jones_1-23_H.schachtii-dehydrated-root_Rep2_ATH1 | | |
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Tissue:
| roots |
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in vivo Treatment:
| At growth stage 1.081.12 (Boyes et al., 2001) plants were randomly allocated into control and treatment groups. Roots of these treatment plants were infected with H. schachtii J2 stage nematodes. Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points. Control plants were mock inoculated with sterile water.
10 days later plants the plants were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar and put back in the growth cabinet for a further 30 minutes before harvesting the tissue. |
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Additional Organism Information:
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Sample Description | Following dehydration treatment and 45 minutes back in the growth cabinet, root material was separated from the green parts of the plant and was immediately frozen in liquid nitrogen. RNA was pooled from 40 plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Spencer-Jones_1-22_H.schachtii-dehydrated-root_Rep1_ATH1 | | |
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Tissue:
| Root |
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in vivo Treatment:
| These plants were innoculated with nematodes and then treated with dehydration stress.
At growth stage 1.081.12 (Boyes et al., 2001) plants were randomly allocated into control and treatment groups. Roots of these treated plants were infected with H. schachtii J2 stage nematodes. Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points.
10 days later plants were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar and put back in the growth cabinet for a further 30 minutes before harvesting the tissue. |
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Additional Organism Information:
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Sample Description | Following mock dehydration treatment and 45 minutes back in the growth cabinet, leaf material was separated from the roots of the plant and was immediately frozen in liquid nitrogen. Root RNA was pooled from 40 plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Spencer-Jones_1-21_H.schachtii-dehydrated-leaf_Rep3_ATH1 | | |
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Tissue:
| leaves |
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in vivo Treatment:
| At growth stage 1.081.12 (Boyes et al., 2001) plants were randomly allocated into control and treatment groups. Roots of these treatment plants were infected with H. schachtii J2 stage nematodes. Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points. Control plants were mock inoculated with sterile water.
10 days later plants the plants were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar and put back in the growth cabinet for a further 30 minutes before harvesting the tissue. |
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Additional Organism Information:
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Sample Description | Following dehydration treatment and 45 minutes back in the growth cabinet, root material was separated from the green parts of the plant and was immediately frozen in liquid nitrogen. RNA was pooled from 40 plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Spencer-Jones_1-20_H.schachtii-dehydrated-leaf_Rep2_ATH1 | | |
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Tissue:
| leaves |
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in vivo Treatment:
| At growth stage 1.081.12 (Boyes et al., 2001) plants were randomly allocated into control and treatment groups. Roots of these treatment plants were infected with H. schachtii J2 stage nematodes. Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points. Control plants were mock inoculated with sterile water.
10 days later plants the plants were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar and put back in the growth cabinet for a further 30 minutes before harvesting the tissue. |
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Additional Organism Information:
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Sample Description | Following dehydration treatment and 45 minutes back in the growth cabinet, root material was separated from the green parts of the plant and was immediately frozen in liquid nitrogen. RNA was pooled from 40 plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Spencer-Jones_1-17_H.schachtii-root_Rep2_ATH1 | | |
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Tissue:
| roots |
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in vivo Treatment:
| At growth stage 1.081.12 (Boyes et al., 2001) plants were randomly allocated into control and treatment groups. Roots of these treatment plants were infected with H. schachtii J2 stage nematodes. Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points. Control plants were mock inoculated with sterile water.
10 days later plants from each group were randomly allocated again into control and dehydration treatment groups. These were control plants so instead of dehydration, plants were carefully lifted off the plate and then immediately placed back on the agar and returned to the growth cabinet for 45 minutes before harvesting the tissue. |
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Additional Organism Information:
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Sample Description | Following mock dehydration treatment and 45 minutes back in the growth cabinet, root material was separated from the green parts of the plant and was immediately frozen in liquid nitrogen. RNA was pooled from 40 plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Spencer-Jones_1-16_H.schachtii-root_Rep1_ATH1 | | |
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Tissue:
| Root |
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in vivo Treatment:
| These plants were innoculated with nematodes, but were not treated with dehydration stress.
At growth stage 1.081.12 (Boyes et al., 2001) plants were randomly allocated into control and treatment groups. Roots of these treated plants were infected with H. schachtii J2 stage nematodes. Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points.
10 days later plants from each group were randomly allocated again into control and dehydration treatment groups. These were control plants so instead of dehydration, plants were carefully lifted off the plate and then immediately placed back on the agar and returned to the growth cabinet for 45 minutes before harvesting the tissue. |
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Additional Organism Information:
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Sample Description | Following mock dehydration treatment and 45 minutes back in the growth cabinet, leaf material was separated from the roots of the plant and was immediately frozen in liquid nitrogen. Root RNA was pooled from 40 plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Spencer-Jones_1-18_H.schachtii-root_Rep3_ATH1 | | |
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Tissue:
| roots |
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in vivo Treatment:
| At growth stage 1.081.12 (Boyes et al., 2001) plants were randomly allocated into control and treatment groups. Roots of these treatment plants were infected with H. schachtii J2 stage nematodes. Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points. Control plants were mock inoculated with sterile water.
10 days later plants from each group were randomly allocated again into control and dehydration treatment groups. These were control plants so instead of dehydration, plants were carefully lifted off the plate and then immediately placed back on the agar and returned to the growth cabinet for 45 minutes before harvesting the tissue. |
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Additional Organism Information:
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Sample Description | Following mock dehydration treatment and 45 minutes back in the growth cabinet, root material was separated from the green parts of the plant and was immediately frozen in liquid nitrogen. RNA was pooled from 40 plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Spencer-Jones_1-15_H.schachtii-leaf_Rep3_ATH1 | | |
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Tissue:
| leaves |
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in vivo Treatment:
| At growth stage 1.081.12 (Boyes et al., 2001) plants were randomly allocated into control and treatment groups. Roots of these treatment plants were infected with H. schachtii J2 stage nematodes. Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points. Control plants were mock inoculated with sterile water.
10 days later plants from each group were randomly allocated again into control and dehydration treatment groups. These were control plants so instead of dehydration, plants were carefully lifted off the plate and then immediately placed back on the agar and returned to the growth cabinet for 45 minutes before harvesting the tissue. |
---|
Additional Organism Information:
| |
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Sample Description | Following mock dehydration treatment and 45 minutes back in the growth cabinet, root material was separated from the green parts of the plant and was immediately frozen in liquid nitrogen. RNA was pooled from 40 plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Spencer-Jones_1-19_H.schachtii-dehydrated-leaf_Rep1_ATH1 | | |
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Tissue:
| Leaf |
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in vivo Treatment:
| These plants were innoculated with nematodes and then treated with dehydration stress.
At growth stage 1.081.12 (Boyes et al., 2001) plants were randomly allocated into control and treatment groups. Roots of these treated plants were infected with H. schachtii J2 stage nematodes. Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points.
10 days later plants were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar and put back in the growth cabinet for a further 30 minutes before harvesting the tissue. |
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Additional Organism Information:
| |
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Sample Description | Following mock dehydration treatment and 45 minutes back in the growth cabinet, leaf material was separated from the roots of the plant and was immediately frozen in liquid nitrogen. Leaf RNA was pooled from 40 plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Spencer-Jones_1-14_H.schachtii-leaf_Rep2_ATH1 | | |
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Tissue:
| leaves |
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in vivo Treatment:
| At growth stage 1.081.12 (Boyes et al., 2001) plants were randomly allocated into control and treatment groups. Roots of these treatment plants were infected with H. schachtii J2 stage nematodes. Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points. Control plants were mock inoculated with sterile water.
10 days later plants from each group were randomly allocated again into control and dehydration treatment groups. These were control plants so instead of dehydration, plants were carefully lifted off the plate and then immediately placed back on the agar and returned to the growth cabinet for 45 minutes before harvesting the tissue. |
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Additional Organism Information:
| |
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Sample Description | Following mock dehydration treatment and 45 minutes back in the growth cabinet, root material was separated from the green parts of the plant and was immediately frozen in liquid nitrogen. RNA was pooled from 40 plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Spencer-Jones_1-13_H.schachtii-leaf_Rep1_ATH1 | | |
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Tissue:
| Leaf |
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in vivo Treatment:
| These plants were innoculated with nematodes, but were not treated with dehydration stress.
At growth stage 1.081.12 (Boyes et al., 2001) plants were randomly allocated into control and treatment groups. Roots of these treated plants were infected with H. schachtii J2 stage nematodes. Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points.
10 days later plants from each group were randomly allocated again into control and dehydration treatment groups. These were control plants so instead of dehydration, plants were carefully lifted off the plate and then immediately placed back on the agar and returned to the growth cabinet for 45 minutes before harvesting the tissue. |
---|
Additional Organism Information:
| |
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Sample Description | Following mock dehydration treatment and 45 minutes back in the growth cabinet, leaf material was separated from the roots of the plant and was immediately frozen in liquid nitrogen. Leaf RNA was pooled from 40 plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Spencer-Jones_1-12_Dehydrated-root_Rep3_ATH1 | | |
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Tissue:
| roots |
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in vivo Treatment:
| At growth stage 1.081.12 (Boyes et al., 2001) plants were randomly allocated into control and nematode treatment groups. These were control plants therefore were mock inoculated with 175µl sterile water.
10 days later plants the plants were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar and put back in the growth cabinet for a further 30 minutes before harvesting the tissue. |
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Additional Organism Information:
| |
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Sample Description | Following dehydration treatment and 45 minutes back in the growth cabinet, root material was separated from the green parts of the plant and was immediately frozen in liquid nitrogen. RNA was pooled from 40 plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Spencer-Jones_1-11_Dehydrated-root_Rep2_ATH1 | | |
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Tissue:
| roots |
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in vivo Treatment:
| At growth stage 1.081.12 (Boyes et al., 2001) plants were randomly allocated into control and nematode treatment groups. These were control plants therefore were mock inoculated with 175µl sterile water.
10 days later plants the plants were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar and put back in the growth cabinet for a further 30 minutes before harvesting the tissue. |
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Additional Organism Information:
| |
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Sample Description | Following dehydration treatment and 45 minutes back in the growth cabinet, root material was separated from the green parts of the plant and was immediately frozen in liquid nitrogen. RNA was pooled from 40 plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Spencer-Jones_1-10_Dehydrated-root_Rep1_ATH1 | | |
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Tissue:
| roots |
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in vivo Treatment:
| At growth stage 1.081.12 (Boyes et al., 2001) plants were randomly allocated into control and nematode treatment groups. These were control plants therefore were mock inoculated with 175µl sterile water.
10 days later plants the plants were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar and put back in the growth cabinet for a further 30 minutes before harvesting the tissue. |
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Additional Organism Information:
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Sample Description | Following dehydration treatment and 45 minutes back in the growth cabinet, root material was separated from the green parts of the plant and was immediately frozen in liquid nitrogen. RNA was pooled from 40 plants. |
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Other Information:
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RNAQC | No |
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RNAReceived | No |
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Protocols for BioSource 1 |
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Slide: Spencer-Jones_1-9_Dehydrated-leaf_Rep3_ATH1 | | |
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Tissue:
| leaves |
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in vivo Treatment:
| At growth stage 1.081.12 (Boyes et al., 2001) plants were randomly allocated into control and nematode treatment groups. These were control plants therefore were mock inoculated with 175µl sterile water.
10 days later plants the plants were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar and put back in the growth cabinet for a further 30 minutes before harvesting the tissue. |
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Additional Organism Information:
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Sample Description | Following dehydration treatment and 45 minutes back in the growth cabinet, root material was separated from the green parts of the plant and was immediately frozen in liquid nitrogen. RNA was pooled from 40 plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Spencer-Jones_1-8_Dehydrated-leaf_Rep2_ATH1 | | |
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Tissue:
| leaves |
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in vivo Treatment:
| At growth stage 1.081.12 (Boyes et al., 2001) plants were randomly allocated into control and nematode treatment groups. These were control plants therefore were mock inoculated with 175µl sterile water.
10 days later plants the plants were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar and put back in the growth cabinet for a further 30 minutes before harvesting the tissue. |
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Additional Organism Information:
| |
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Sample Description | Following dehydration treatment and 45 minutes back in the growth cabinet, root material was separated from the green parts of the plant and was immediately frozen in liquid nitrogen. RNA was pooled from 40 plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Spencer-Jones_1-7_Dehydrated-leaf_Rep1_ATH1 | | |
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Tissue:
| Leaf |
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in vivo Treatment:
| These plants were not innoculated with nematodes, but were treated with dehydration stress.
At growth stage 1.081.12 (Boyes et al., 2001) plants were randomly allocated into control and nematode treatment groups. These were control plants therefore were mock inoculated with 175µl sterile water.
10 days later plants the plants were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar and put back in the growth cabinet for a further 30 minutes before harvesting the tissue. |
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Additional Organism Information:
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Sample Description | Following mock dehydration treatment and 45 minutes back in the growth cabinet, leaf material was separated from the roots of the plant and was immediately frozen in liquid nitrogen. Leaf RNA was pooled from 40 plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Spencer-Jones_1-6_Control-root_Rep3_ATH1 | | |
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Tissue:
| roots |
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Additional Organism Information:
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Sample Description | At growth stage 1.081.12 (Boyes et al., 2001) plants were randomly allocated into control and nematode treatment groups. These were control plants therefore were mock inoculated with 175µl sterile water.
10 days later plants from each group were randomly allocated again into control and dehydration treatment groups. These were control plants so instead of dehydration, plants were carefully lifted off the plate and then immediately placed back on the agar and returned to the growth cabinet for 45 minutes before harvesting the tissue. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Spencer-Jones_1-5_Control-root_Rep2_ATH1 | | |
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Tissue:
| roots |
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Additional Organism Information:
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Sample Description | At growth stage 1.081.12 (Boyes et al., 2001) plants were randomly allocated into control and nematode treatment groups. These were control plants therefore were mock inoculated with 175µl sterile water.
10 days later plants from each group were randomly allocated again into control and dehydration treatment groups. These were control plants so instead of dehydration, plants were carefully lifted off the plate and then immediately placed back on the agar and returned to the growth cabinet for 45 minutes before harvesting the tissue. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Spencer-Jones_1-4_Control-root_Rep1_ATH1 | | |
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Tissue:
| Root |
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in vivo Treatment:
| These control plants underwent similar handling procedures to the treated plants in order to minimise differences in gene response:
At growth stage 1.081.12 (Boyes et al., 2001) plants were randomly allocated into control and nematode treatment groups. These were control plants therefore were mock inoculated with 175µl sterile water.
10 days later plants from each group were randomly allocated again into control and dehydration treatment groups. These were control plants so instead of dehydration, plants were carefully lifted off the plate and then immediately placed back on the agar and returned to the growth cabinet for 45 minutes before harvesting the tissue. |
---|
Additional Organism Information:
| |
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Sample Description | Following mock dehydration treatment and 45 minutes back in the growth cabinet, root material was separated from the leaves of the plant and was immediately frozen in liquid nitrogen. Root RNA was pooled from 40 plants. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Spencer-Jones_1-1_Control-leaf_Rep1_ATH1 | | |
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Tissue:
| Leaf |
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in vivo Treatment:
| These control plants underwent similar handling procedures to the treated plants in order to minimise differences in gene response:
At growth stage 1.081.12 (Boyes et al., 2001) plants were randomly allocated into control and nematode treatment groups. These were control plants therefore were mock inoculated with 175µl sterile water.
10 days later plants from each group were randomly allocated again into control and dehydration treatment groups. These were control plants so instead of dehydration, plants were carefully lifted off the plate and then immediately placed back on the agar and returned to the growth cabinet for 45 minutes before harvesting the tissue. |
---|
Additional Organism Information:
| |
---|
Sample Description | Following mock dehydration treatment and 45 minutes back in the growth cabinet, leaf material was separated from the roots of the plant and was immediately frozen in liquid nitrogen. RNA was pooled from 40 plants. |
---|
Other Information:
| |
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|
Protocols for BioSource 1 |
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Slide: Spencer-Jones_1-2_Control-leaf_Rep2_ATH1 | | |
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Tissue:
| leaves |
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Additional Organism Information:
| |
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Sample Description | At growth stage 1.081.12 (Boyes et al., 2001) plants were randomly allocated into control and nematode treatment groups. These were control plants therefore were mock inoculated with 175µl sterile water.
10 days later plants from each group were randomly allocated again into control and dehydration treatment groups. These were control plants so instead of dehydration, plants were carefully lifted off the plate and then immediately placed back on the agar and returned to the growth cabinet for 45 minutes before harvesting the tissue. |
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Other Information:
| |
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|
Protocols for BioSource 1 |
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Slide: Spencer-Jones_1-3_Control-leaf_Rep3_ATH1 | | |
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Tissue:
| leaves |
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Additional Organism Information:
| |
---|
Sample Description | At growth stage 1.081.12 (Boyes et al., 2001) plants were randomly allocated into control and nematode treatment groups. These were control plants therefore were mock inoculated with 175µl sterile water.
10 days later plants from each group were randomly allocated again into control and dehydration treatment groups. These were control plants so instead of dehydration, plants were carefully lifted off the plate and then immediately placed back on the agar and returned to the growth cabinet for 45 minutes before harvesting the tissue. |
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Other Information:
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|
Protocols for BioSource 1 |
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Problems? Comments? Suggestions? Contact the Affymetrix Team