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Experiment: Transcriptome of Arabidopsis APS kinase null mutants
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-457
The aim of the experiment is to look at transcriptional changes in null mutants of APS kinase. There are four APS kinase isoforms in Arabidopsis, we are interested in the double mutant APK1/APK2, created by crossing two single salk lines. The transcriptomes of WT (Col-0) plants and the double knockout (ab) will be compared. The plants were grown under controlled environment conditions of 10h day, 14h night, 22 degrees centigrade, 60% humidity for five weeks. 10 whole rosettes were pooled for each replicate, there are three replicates for each genotype.
About the ExperimenterName: | Dr Sarah Mugford |
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Head of Lab Name: | Dr Stanislav Kopriva |
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Lab:
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Address: | Dept. of Metabolic Biology John Innes Centre Norwich Research Park Colney Lane Norwich
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Postcode:
| NR4 7UH |
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Country:
| UK |
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| Telephone Number:
| 01603450252 |
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Fax Number:
| 01603450045 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| genetic_modification_design |
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Number of Slides: | 6 |
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| Experimental Parameters:
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parameter | gene_knock_out |
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Quality Control Measures Taken:
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Mugford: Transcriptome of Arabidopsis APS kinase null mutants_genome
Slide: Mugford_1-1_Col0-control_Rep1_ATH1 | | |
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Tissue:
| whole rosette |
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Additional Organism Information:
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Separation technique | Rosettes were harvested by cutting with sharp scissors at the top of the main root |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Mugford_1-2_Col0-control_Rep2_ATH1 | | |
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Tissue:
| whole rosette |
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Additional Organism Information:
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Separation technique | Rosettes were harvested by cutting with sharp scissors at the top of the main root |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Mugford_1-3_Col0-control_Rep3_ATH1 | | |
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Tissue:
| whole rosette |
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Additional Organism Information:
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Separation technique | Rosettes were harvested by cutting with sharp scissors at the top of the main root |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Mugford_1-4_ab-double-knockout_Rep1_ATH1 | | |
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Tissue:
| whole rosette |
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Additional Organism Information:
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Separation technique | Rosettes were harvested by cutting with sharp scissors at the top of the main root |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Mugford_1-5_ab-double-knockout_Rep2_ATH1 | | |
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Tissue:
| whole rosette |
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Additional Organism Information:
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Separation technique | Rosettes were harvested by cutting with sharp scissors at the top of the main root |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Mugford_1-6_ab-double-knockout_Rep3_ATH1 | | |
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Tissue:
| whole rosette |
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Additional Organism Information:
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Separation technique | Rosettes were harvested by cutting with sharp scissors at the top of the main root |
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Other Information:
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Protocols for BioSource 1 |
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