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Experiment: Incompatible interaction Plasmodiophora-Arabidopsis
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-400
A monogenetic inherited resistance phenotype to Plasmodiophora brassicae has been found in Arabidopsis ecotype Tsu-0. The dominantly inherited RPB1 gene mediates a hypersensitive-like response reaction. Inoculated roots of the resistant line will be compared to control roots at different time points after inoculation in order to get insights into the incompatible interaction. The corresponding dataset has been published (Siemens et al. 2006, MPMI). Furthermore, crossing experiments of the line Tsu-0 with mutant lines of salicylic acid, jasmonic acid and ethylene revealed no influence of these hormones in the resistance reaction. Furthermore, mutations of sgt1b and rar1 showed no influence on RPB1 mediated resistance, but a mutation of sgt1a (At4g23570) reveals to be epistatic to RPB1, indicating to protein ubiquitination and protein degradation as important part of clubroot resistance mechanism. The RPB1 locus has been localized to a region on chromosome 1 with 14 coding sequences according to the sequence of ecotype Columbia. One of these genes is polymorphic between resistant and susceptible ecotypes and encodes for a protein interacting with a SCF-complex. Therefor this gene is probably identical to RPB1.
About the ExperimenterName: | Dr. Johannes Siemens |
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Head of Lab Name: | Dr. Johannes Siemens |
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Lab:
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Institute:
| Technical University Dresden |
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Address: | Technical University Dresden Dept. Biology Molecular Biotechnology Dresden
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Postcode:
| 01062 |
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Country:
| Germany |
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| Telephone Number:
| +49 351 463 36789 |
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Fax Number:
| +49 351 463 37749 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
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Number of Slides: | 6 |
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| Experimental Parameters:
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exp-factors | infect |
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Quality Control Measures Taken:
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References:
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| Other Information:
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other factors | Infected with Plasmodiophora brassicae |
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Slides in this Experiment
Hybridisation Set: Siemens: Incompatible interaction Plasmodiophora-Arabidopsis_genome
Slide: Siemens_1-1_Tsu10dpi_Rep1_ATH1 | | |
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Tissue:
| roots |
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Additional Organism Information:
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Sample Description | The plants were dug up 28 days after germination and soil was removed from the roots with the help of brushes. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Siemens_1-2_Tsu10dpi_Rep2_ATH1 | | |
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Tissue:
| roots |
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Additional Organism Information:
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Sample Description | The plants were dug up 28 days after germination and soil was removed from the roots with the help of brushes. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Siemens_1-3_Tsu14dpi_Rep1_ATH1 | | |
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Tissue:
| roots |
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in vivo Treatment:
| Fourteen day old plants were inoculated by injecting the soil around the plant with 2 ml of a resting spore suspension with a concentration of 10 to 6 spores/ml of the pathogen Plasmodiophora brassicae isolate "e3". |
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Additional Organism Information:
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Sample Description | The plants were dug up 14 days after inoculation (28 days after germination) by soil was removed from the roots with the help of brushes. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Siemens_1-4_Tsu24dpg_Rep1_ATH1 | | |
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Tissue:
| roots |
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Additional Organism Information:
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Sample Description | The plants were dug up 28 days after germination and soil was removed from the roots with the help of brushes. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Siemens_1-5_Tsu28dpg_Rep1_ATH1 | | |
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Tissue:
| roots |
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Additional Organism Information:
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Sample Description | The plants were dug up 28 days after germination and soil was removed from the roots with the help of brushes. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Siemens_1-6_Tsu24dpg_Rep2_ATH1 | | |
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Tissue:
| root |
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Additional Organism Information:
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Sample Description | Plants were grown in soil for 24 days |
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Other Information:
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Protocols for BioSource 1 |
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