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Experiment: Identification of AtATM dependent X-ray inducible transcript
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-370
ATM plays an important role in the response of plants to forms of DNA damage that cause DNA double strand breaks. Arabidopsis ATM has been shown to be important for the transcriptional induction of several DNA repair genes including AtRAD51 (Garcia et al 2003 Plant Cell 15, 119-132). In our experiment we compare the transcript profiles of wild type and mutant plants exposed to 10Gy X-rays (dose rate ~0.8 Gy/min). Plants will be grown on 0.5*MS + 1%sucrose at 22degC under short (9h) day conditions and treated after 5h light. We will irradiate wild type (Col-0) and atatm-3 mutant (N589805) seedlings at growth stage 1.06 and harvest tissue 1h post irradiation. RNA will be isolated using the SV RNA isolation kit (Promega). Comparison of the transcript profiles will then allow AtATM-dependent X-ray inducible transcripts to be identified.
About the ExperimenterName: | Dr Chris West |
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Head of Lab Name: | Dr Chris West |
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Lab:
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Institute:
| University of Leeds |
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Address: | Centre for Plant Sciences University of Leeds Woodhouse Lane Leeds West Yorkshire
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Postcode:
| LS2 9JT |
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Country:
| UK |
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| Telephone Number:
| 01133432915 |
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Fax Number:
| 0113 3433144 |
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are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| genetic_modification_design |
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Number of Slides: | 6 |
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| Experimental Parameters:
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parameter | gene_knock_out |
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Quality Control Measures Taken:
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: West: Identification of AtATM dependent X-ray inducible transcript_genome
Slide: West_2-1_Col0_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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treatment | Fourteen day old seedlings were exposed, whilst on the plates, to a 10 Gy dose of X-rays over the course of 7.5 minutes using a 320 kV X-ray system. Plants were harvested 1 hour following treatment. |
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Additional Organism Information:
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sampledescription | Whole seedlings (14 days old) |
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Other Information:
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Protocols for BioSource 1 |
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Slide: West_2-2_Col0_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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treatment | Fourteen day old seedlings were exposed, whilst on the plates, to a 10 Gy dose of X-rays over the course of 7.5 minutes using a 320 kV X-ray system. Plants were harvested 1 hour following treatment. |
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Additional Organism Information:
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sampledescription | Whole seedlings (14 days old) |
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Other Information:
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Protocols for BioSource 1 |
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Slide: West_2-3_Col0_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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treatment | Fourteen day old seedlings were exposed, whilst on the plates, to a 10 Gy dose of X-rays over the course of 7.5 minutes using a 320 kV X-ray system. Plants were harvested 1 hour following treatment. |
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Additional Organism Information:
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sampledescription | Whole seedlings (14 days old) |
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Other Information:
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Protocols for BioSource 1 |
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Slide: West_2-4_atm_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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treatment | Fourteen day old seedlings were exposed, whilst on the plates, to a 10 Gy dose of X-rays over the course of 7.5 minutes using a 320 kV X-ray system. Plants were harvested 1 hour following treatment. |
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Additional Organism Information:
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sampledescription | Whole seedlings (14 days old) |
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Other Information:
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Protocols for BioSource 1 |
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Slide: West_2-5_atm_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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treatment | Fourteen day old seedlings were exposed, whilst on the plates, to a 10 Gy dose of X-rays over the course of 7.5 minutes using a 320 kV X-ray system. Plants were harvested 1 hour following treatment. |
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Additional Organism Information:
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sampledescription | Whole seedlings (14 days old) |
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Other Information:
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Protocols for BioSource 1 |
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Slide: West_2-6_atm_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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treatment | Fourteen day old seedlings were exposed, whilst on the plates, to a 10 Gy dose of X-rays over the course of 7.5 minutes using a 320 kV X-ray system. Plants were harvested 1 hour following treatment. |
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Additional Organism Information:
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sampledescription | Whole seedlings (14 days old) |
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Other Information:
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|
Protocols for BioSource 1 |
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