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Experiment: Etiolated seedling of the exo70A1 mutant versus wild type
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-435
The EXO70A1 protein is supposed to be a subunit of putative plant exocyst complex. We characterized two independent T-DNA insertional mutants of the AtEXO70A1 gene (exo70A1-1 and exo70A1-2). Heterozygous EXO70A1/exo70A1 plants appear normal and segregate in a 1:2:1 ratio, suggesting that neither male nor female gametophytes are affected by the EXO70A1 disruption. However, both exo70A1-1 and exo70A1-2 homozygotes exhibit a suite of phenotypic defects. Polar growth of root hairs and stigmatic papillae is disturbed. Hypocotyls of etiolated seedlings are shorter. Organs are generally smaller, plants show loss of apical dominance and an indeterminate growth where instead of floral meristems new lateral inflorescences are initiated in a reiterative manner. Both exo70A1 mutants have dramatically reduced fertility. These results suggest that the putative exocyst subunit EXO70A1 is involved in cell and organ morphogenesis.
About the ExperimenterName: | PhD. Lukas Synek |
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Head of Lab Name: | RNDr. Viktor Zarsky |
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Lab:
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Address: | Institute of Experimental Botany Academy of Sciences of the Czech Republic Rozvojova 263 Prague 6
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Postcode:
| 165 02 |
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Country:
| Czech Republic |
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| Telephone Number:
| 420225106458 |
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| About this ExperimentExperiment Type:
| genetic_modification_design |
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Number of Slides: | 8 |
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| Experimental Parameters:
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parameter | gene_knock_out |
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Quality Control Measures Taken:
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no-plants-pooled | |
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References:
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Slides in this Experiment
Hybridisation Set: Synek: Etiolated seedling of the exo70A1 mutant versus wild type_genome
Slide: Synek_1-1_wt-etiolated_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | etiolated 7-day-old seedlings with elongated hypocotyls (20 mm) |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Synek_1-2_wt-etiolated_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | etiolated 7-day-old seedlings with elongated hypocotyls (20 mm) |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Synek_1-3_exo70A1-mutant-etiolated_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | etiolated 7-day-old seedlings with elongated hypocotyls (8 mm) |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Synek_1-4_exo70A1-mutant-etiolated_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | etiolated 7-day-old seedlings with elongated hypocotyls (8 mm) |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Synek_1-5_wt_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | wild-type plants at the stage 6.00, typical for continuous light conditions |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Synek_1-6_wt_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | wild-type plants at the stage 6.00, typical for continuous light conditions |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Synek_1-7_exo70A1-mutant_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Synek_1-8_exo70A1-mutant_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Other Information:
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Protocols for BioSource 1 |
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