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Experiment: Oligogalacturonide treatment of Arabidopsis seedlings
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-409
To assess the contribution to defenses against necrotrophic fungal pathogens that may be mediated by recognition of oligogalacturonides (OGs), cell wall fragments released by the activity of fungal polygalacturonases, we treated seedlings with OGs and assayed transcript levels 1 and 6 hours after addition of OGs to culture medium. For each sample, approximately 30 seedlings were grown in shallow liquid MS medium for 10 days. Plants were then treated with either 200 ug/ml OGs or, for control plants, an equal volume of water. Three replicate samples were assayed for each treatment.
About the ExperimenterName: | Dr. Julia Dewdney |
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Head of Lab Name: | Dr. Frederick Ausubel |
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Lab:
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Institute:
| Massachusetts General Hospital |
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Address: | Dept. of Molecular Biology Massachusetts General Hospital Simches 7700 185 Cambridge St. Boston, MA
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Postcode:
| 02114 |
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Country:
| USA |
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| Telephone Number:
| 617-643-3325 |
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Fax Number:
| 617 643 3050 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| time_series_design; compound_based_treatment_design |
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Number of Slides: | 18 |
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| Experimental Parameters:
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parameter | compound_based_treatment |
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parameter | timepoint |
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Quality Control Measures Taken:
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Dewdney: Oligogalacturonide treatment of Arabidopsis seedlings_genome
Slide: Dewdney_1-1_Flg22-1h_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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treatment | Flg22, final concentration 1 uM, was added to the medium on day 10. |
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Other Information:
| Columbia, USA Seed stock maintained in lab for 20 years. |
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Protocols for BioSource 1 |
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Slide: Dewdney_1-2_Flg22-1h_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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treatment | Flg22, final concentration 1 uM, was added to the medium on day 10. |
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Other Information:
| Columbia, USA Seed stock maintained in lab for 20 years. |
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Protocols for BioSource 1 |
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Slide: Dewdney_1-3_Flg22-1h_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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treatment | Flg22, final concentration 1 uM, was added to the medium on day 10. |
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Other Information:
| Columbia, USA Seed stock maintained in lab for 20 years. |
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Protocols for BioSource 1 |
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Slide: Dewdney_1-4_Flg22-3h_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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treatment | Flg22, final concentration 1 uM, was added to the medium on day 10. |
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Other Information:
| Columbia, USA Seed stock maintained in lab for 20 years. |
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Protocols for BioSource 1 |
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Slide: Dewdney_1-5_Flg22-3h_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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treatment | Flg22, final concentration 1 uM, was added to the medium on day 10. |
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Other Information:
| Columbia, USA Seed stock maintained in lab for 20 years. |
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Protocols for BioSource 1 |
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Slide: Dewdney_1-6_Flg22-3h_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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treatment | Flg22, final concentration 1 uM, was added to the medium on day 10. |
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Other Information:
| Columbia, USA Seed stock maintained in lab for 20 years. |
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Protocols for BioSource 1 |
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Slide: Dewdney_1-7_H20-1h_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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treatment | water was added to the medium on day 10 |
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Other Information:
| Columbia, USA Seed stock maintained in lab for 20 years. |
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Protocols for BioSource 1 |
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Slide: Dewdney_1-8_H20-1h_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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treatment | water was added to the medium on day 10 |
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Other Information:
| Columbia, USA Seed stock maintained in lab for 20 years. |
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Protocols for BioSource 1 |
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Slide: Dewdney_1-9_H20-1h_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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treatment | water was added to the medium on day 10 |
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Other Information:
| Columbia, USA Seed stock maintained in lab for 20 years. |
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Protocols for BioSource 1 |
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Slide: Dewdney_1-10_H20-3h_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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treatment | water was added to the medium on day 10 |
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Other Information:
| Columbia, USA Seed stock maintained in lab for 20 years. |
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Protocols for BioSource 1 |
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Slide: Dewdney_1-11_H20-3h_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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treatment | water was added to the medium on day 10 |
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Other Information:
| Columbia, USA Seed stock maintained in lab for 20 years. |
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Protocols for BioSource 1 |
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Slide: Dewdney_1-12_H20-3h_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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treatment | water was added to the medium on day 10 |
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Other Information:
| Columbia, USA Seed stock maintained in lab for 20 years. |
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Protocols for BioSource 1 |
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Slide: Dewdney_1-13_OGs-1h_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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treatment | 50 ug/ml oligogalacturonides, degree of polymerization 10-15, were added to the medium on day 10. |
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Other Information:
| Columbia, USA Seed stock maintained in lab for 20 years. |
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Protocols for BioSource 1 |
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Slide: Dewdney_1-14_OGs-1h_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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treatment | 50 ug/ml oligogalacturonides, degree of polymerization 10-15, were added to the medium on day 10. |
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Other Information:
| Columbia, USA Seed stock maintained in lab for 20 years. |
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Protocols for BioSource 1 |
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Slide: Dewdney_1-15_OGs-1h_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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treatment | 50 ug/ml oligogalacturonides, degree of polymerization 10-15, were added to the medium on day 10. |
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Other Information:
| Columbia, USA Seed stock maintained in lab for 20 years. |
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|
Protocols for BioSource 1 |
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Slide: Dewdney_1-16_OGs-3h_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| |
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treatment | 50 ug/ml oligogalacturonides, degree of polymerization 10-15, were added to the medium on day 10. |
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Other Information:
| Columbia, USA Seed stock maintained in lab for 20 years. |
---|
|
Protocols for BioSource 1 |
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Slide: Dewdney_1-17_OGs-3h_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| |
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treatment | 50 ug/ml oligogalacturonides, degree of polymerization 10-15, were added to the medium on day 10. |
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Other Information:
| Columbia, USA Seed stock maintained in lab for 20 years. |
---|
|
Protocols for BioSource 1 |
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Slide: Dewdney_1-18_OGs-3h_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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treatment | 50 ug/ml oligogalacturonides, degree of polymerization 10-15, were added to the medium on day 10. |
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Other Information:
| Columbia, USA Seed stock maintained in lab for 20 years. |
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|
Protocols for BioSource 1 |
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Problems? Comments? Suggestions? Contact the Affymetrix Team