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Experiment: Transcriptome analysis of DELAY OF GERMINATION1 (DOG1) mutant during seed maturation
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-614
Seed dormancy controls the start of a plant's life cycle by preventing germination of a viable seed in an unfavorable season. The molecular mechanisms controlling seed dormancy are not well understood. DELAY OF GERMINATION 1 (DOG1) has been identified as a major Quantitative Trait Locus (QTL) for seed dormancy in a Recombinant Inbred Line (RIL) population between the lowly dormant accession Landsberg erecta (Ler) and the high dormant accession Cape Verde Islands (Cvi) (Alonso Blanco et al., 2003). DOG1 is a key regulator of seed dormancy because dog1 mutants are completely non-dormant and do not show any obvious pleiotropic phenotypes, apart from a reduced seed longevity (Bentsink et al., 2006). We compared the transcriptome of dog1-1 mutant with that of its wild-type background NIL DOG1_Cvi on siliques at mid-maturation phase when DOG1 is highly expressed. This analysis shows the influence of DOG1 on gene expression related to dormancy induction during seed maturation.
About the ExperimenterName: | Dr. Kazumi Nakabayashi |
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Head of Lab Name: | Dr. Wim Soppe |
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Lab:
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Address: | Max Planck Institute for Plant Breedinf Research Carl-von-Linne-Weg 10 Cologne
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Postcode:
| 50829 |
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Country:
| Germany |
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are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| genetic_modification_design |
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Number of Slides: | 4 |
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| Experimental Parameters:
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parameter | gene_knock_out |
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Quality Control Measures Taken:
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References:
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Slides in this Experiment
Hybridisation Set: Nakabayashi: Transcriptome analysis of DELAY OF GERMINATION1 (DOG1) mutant during seed maturation
Slide: Nakabayashi_614-4_mutant_Rep2_ATH1 | | |
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Tissue:
| siliques |
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Additional Organism Information:
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Sample Description | In the growth condition described above, bolting/flowering started nearly the same time in both genotypes, and seeds were matured fully at 15 days after pollination. Siliques were harvested after 12-13 days after pollination. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Nakabayashi_614-1_background_Rep1_ATH1 | | |
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Tissue:
| siliques |
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Additional Organism Information:
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Sample Description | In the growth condition described above, bolting/flowering started nearly the same time in both genotypes, and seeds were matured fully at 15 days after pollination. Siliques were harvested after 12-13 days after pollination. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Nakabayashi_614-2_background_Rep2_ATH1 | | |
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Tissue:
| siliques |
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Additional Organism Information:
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Sample Description | In the growth condition described above, bolting/flowering started nearly the same time in both genotypes, and seeds were matured fully at 15 days after pollination. Siliques were harvested after 12-13 days after pollination. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Nakabayashi_614-3_mutant_Rep1_ATH1 | | |
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Tissue:
| siliques |
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Additional Organism Information:
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Sample Description | In the growth condition described above, bolting/flowering started nearly the same time in both genotypes, and seeds were matured fully at 15 days after pollination. Siliques were harvested after 12-13 days after pollination. |
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Other Information:
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Protocols for BioSource 1 |
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