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Experiment: Microarray Analysis of cpl3 Mutant
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-657
An Arabidopsis (Arabidopsis thaliana) multigene family (predicted to be more than 20 members) encodes plant C-terminal domain (CTD) phosphatases that dephosphorylate Ser residues in tandem heptad repeat sequences of the RNA polymerase II C terminus. CTD phosphatase-like (CPL) isoform 3 (CPL3)is a regulator of osmotic stress and abscisic acid (ABA) signaling. Recombinant CPL3 interacts with AtRAP74, an Arabidopsis ortholog of a FCP1-interacting TFIIF subunit. A CPL3 C-terminal fragment that contains the BRCT domain mediates molecular interaction with AtRAP74. Consistent with its predicted role in transcriptional regulation, green fluorescent protein fusion protein of CPL3 co-localizes to the nucleus with AtRAP74. cpl3 mutations that eliminate the BRCT or FCP1 homology domain cause ABA hyperactivation of the stress-inducible RD29a promoter. Hence, CPL3 is an isoform that specially facilitates ABA signaling.Reference:Bang et.al., 2006.
About the ExperimenterName: | Hisashi koiwa |
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Head of Lab Name: | Hisashi koiwa |
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Lab:
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Address: | Department of Horticultural Sciences, College of Agriculture and Life Science, Texas A & M University 202 Horticulture/Forest Science Building, , College Station, Texas, TX 77843-2133, UNITED STATES
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Postcode:
| TX 77843-2133 |
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Country:
| UNITED STATES |
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| Telephone Number:
| +1 979-845-5282 |
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Fax Number:
| +1 979-845-0627 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| genetic_modification |
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Number of Slides: | 6 |
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| Experimental Parameters:
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parameter | genetic_modification |
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Quality Control Measures Taken:
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References:
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Reference | Bang et.al., 2006. |
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Slides in this Experiment
Hybridisation Set: koiwa: Microarray Analysis of cpl3 Mutant
Slide: koiwa_657-1_C24_WT_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Whole plant is used for sampling. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: koiwa_657-2_C24_WT_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Whole plant is used for sampling. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: koiwa_657-3_C24_WT_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Whole plant is used for sampling. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: koiwa_657-4_cpl1-3_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Whole plant is used for sampling. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: koiwa_657-5_cpl1-3_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Whole plant is used for sampling. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: koiwa_657-6_cpl1-3_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Whole plant is used for sampling. |
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Other Information:
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Protocols for BioSource 1 |
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