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Experiment: Expression analysis on low phosphate conditions
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-648
Low phosphate conditions induce massive changes in the root system architecture (RSA). Our aim was to find differences in the expression pattern of Pi starvation related genes under high and low phosphate conditions in the wild type versus starvation hypersensitive and insensitive (double-) mutants pdr2 and lpr1lpr2, respectively. For this, we used RNA from root tissue of 5 days old seedlings which were grown for 4 days on high phosphate medium and subsequently transferred to low phosphate medium for 20h.
About the ExperimenterName: | Mr Jens Mueller |
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Head of Lab Name: | Prof Steffen Abel |
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Lab:
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Address: | Department of Molecular Signal Processing, Leibniz Institute of Plant Biochemistry Weinberg 3 , Halle, Sachsen-Anhalt, 06120, GERMANY
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Postcode:
| 06120 |
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Country:
| GERMANY |
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| Telephone Number:
| +4934555821240 |
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are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
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co-authors in the work.
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| About this ExperimentExperiment Type:
| starvation |
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Number of Slides: | 18 |
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| Experimental Parameters:
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parameter | starvation |
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Quality Control Measures Taken:
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Mueller: Expression analysis on low phosphate conditions
Slide: Mueller_648-18_lpr1lpr2(-)_Rep3_ATH1 | | |
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Tissue:
| whole root |
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in vivo Treatment:
| Plants were germinated and grown for 4 days on medium with high phosphate (2,5mM / (+)) and subsequently transferred for 20h to either medium with no phosphate (0mM / (-)) or to medium with high phosphate (control plants). |
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Additional Organism Information:
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Sample Description | Samples were derived from roots of 5 days old seedlings. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Mueller_648-17_pdr2(-)_Rep3_ATH1 | | |
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Tissue:
| whole root |
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in vivo Treatment:
| Plants were germinated and grown for 4 days on medium with high phosphate (2,5mM / (+)) and subsequently transferred for 20h to either medium with no phosphate (0mM / (-)) or to medium with high phosphate (control plants). |
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Additional Organism Information:
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Sample Description | Samples were derived from roots of 5 days old seedlings. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Mueller_648-16_Col (-)_Rep3_ATH1 | | |
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Tissue:
| whole root |
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in vivo Treatment:
| Plants were germinated and grown for 4 days on medium with high phosphate (2,5mM / (+)) and subsequently transferred for 20h to either medium with no phosphate (0mM / (-)) or to medium with high phosphate (control plants). |
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Additional Organism Information:
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Sample Description | Samples were derived from roots of 5 days old seedlings. |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Mueller_648-15_lpr1lpr2(+)_Rep3_ATH1 | | |
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Tissue:
| whole root |
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in vivo Treatment:
| Plants were germinated and grown for 4 days on medium with high phosphate (2,5mM / (+)) and subsequently transferred for 20h to either medium with no phosphate (0mM / (-)) or to medium with high phosphate (control plants). |
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Additional Organism Information:
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Sample Description | Samples were derived from roots of 5 days old seedlings. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Mueller_648-14_pdr2(+)_Rep3_ATH1 | | |
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Tissue:
| whole root |
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in vivo Treatment:
| Plants were germinated and grown for 4 days on medium with high phosphate (2,5mM / (+)) and subsequently transferred for 20h to either medium with no phosphate (0mM / (-)) or to medium with high phosphate (control plants). |
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Additional Organism Information:
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Sample Description | Samples were derived from roots of 5 days old seedlings. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Mueller_648-13_Col(+)_Rep3_ATH1 | | |
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Tissue:
| whole root |
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in vivo Treatment:
| Plants were germinated and grown for 4 days on medium with high phosphate (2,5mM / (+)) and subsequently transferred for 20h to either medium with no phosphate (0mM / (-)) or to medium with high phosphate (control plants). |
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Additional Organism Information:
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Sample Description | Samples were derived from roots of 5 days old seedlings. |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Mueller_648-12_lpr1lpr2(-)_Rep2_ATH1 | | |
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Tissue:
| whole root |
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in vivo Treatment:
| Plants were germinated and grown for 4 days on medium with high phosphate (2,5mM / (+)) and subsequently transferred for 20h to either medium with no phosphate (0mM / (-)) or to medium with high phosphate (control plants). |
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Additional Organism Information:
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Sample Description | Samples were derived from roots of 5 days old seedlings. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Mueller_648-11_pdr2(-) _Rep2_ATH1 | | |
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Tissue:
| whole root |
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in vivo Treatment:
| Plants were germinated and grown for 4 days on medium with high phosphate (2,5mM / (+)) and subsequently transferred for 20h to either medium with no phosphate (0mM / (-)) or to medium with high phosphate (control plants). |
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Additional Organism Information:
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Sample Description | Samples were derived from roots of 5 days old seedlings. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Mueller_648-10_Col(-)_Rep2_ATH1 | | |
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Tissue:
| whole root |
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in vivo Treatment:
| Plants were germinated and grown for 4 days on medium with high phosphate (2,5mM / (+)) and subsequently transferred for 20h to either medium with no phosphate (0mM / (-)) or to medium with high phosphate (control plants). |
---|
Additional Organism Information:
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Sample Description | Samples were derived from roots of 5 days old seedlings. |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Mueller_648-9_lpr1lpr2(+)_Rep2_ATH1 | | |
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Tissue:
| whole root |
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in vivo Treatment:
| Plants were germinated and grown for 4 days on medium with high phosphate (2,5mM / (+)) and subsequently transferred for 20h to either medium with no phosphate (0mM / (-)) or to medium with high phosphate (control plants). |
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Additional Organism Information:
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Sample Description | Samples were derived from roots of 5 days old seedlings. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Mueller_648-8_pdr2(+)_Rep2_ATH1 | | |
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Tissue:
| whole root |
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in vivo Treatment:
| Plants were germinated and grown for 4 days on medium with high phosphate (2,5mM / (+)) and subsequently transferred for 20h to either medium with no phosphate (0mM / (-)) or to medium with high phosphate (control plants). |
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Additional Organism Information:
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Sample Description | Samples were derived from roots of 5 days old seedlings. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Mueller_648-7_Col(+)_Rep2_ATH1 | | |
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Tissue:
| whole root |
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in vivo Treatment:
| Plants were germinated and grown for 4 days on medium with high phosphate (2,5mM / (+)) and subsequently transferred for 20h to either medium with no phosphate (0mM / (-)) or to medium with high phosphate (control plants). |
---|
Additional Organism Information:
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Sample Description | Samples were derived from roots of 5 days old seedlings. |
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Other Information:
| |
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Mueller_648-6_lpr1lpr2(-)_Rep1_ATH1 | | |
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Tissue:
| whole root |
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in vivo Treatment:
| Plants were germinated and grown for 4 days on medium with high phosphate (2,5mM / (+)) and subsequently transferred for 20h to either medium with no phosphate (0mM / (-)) or to medium with high phosphate (control plants). |
---|
Additional Organism Information:
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Sample Description | Samples were derived from roots of 5 days old seedlings. |
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Other Information:
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|
Protocols for BioSource 1 |
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Slide: Mueller_648-5_pdr2(-)_Rep1_ATH1 | | |
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Tissue:
| whole root |
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in vivo Treatment:
| Plants were germinated and grown for 4 days on medium with high phosphate (2,5mM / (+)) and subsequently transferred for 20h to either medium with no phosphate (0mM / (-)) or to medium with high phosphate (control plants). |
---|
Additional Organism Information:
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Sample Description | Samples were derived from roots of 5 days old seedlings. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Mueller_648-4_Col(-)_Rep1_ATH1 | | |
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Tissue:
| whole root |
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in vivo Treatment:
| Plants were germinated and grown for 4 days on medium with high phosphate (2,5mM / (+)) and subsequently transferred for 20h to either medium with no phosphate (0mM / (-)) or to medium with high phosphate (control plants). |
---|
Additional Organism Information:
| |
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Sample Description | Samples were derived from roots of 5 days old seedlings. |
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Other Information:
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ecotype_habitat | |
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|
Protocols for BioSource 1 |
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Slide: Mueller_648-3_lpr1lpr2(+)_Rep1_ATH1 | | |
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Tissue:
| whole root |
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in vivo Treatment:
| Plants were germinated and grown for 4 days on medium with high phosphate (2,5mM / (+)) and subsequently transferred for 20h to either medium with no phosphate (0mM / (-)) or to medium with high phosphate (control plants). |
---|
Additional Organism Information:
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Sample Description | Samples were derived from roots of 5 days old seedlings. |
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Other Information:
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|
Protocols for BioSource 1 |
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Slide: Mueller_648-2_pdr2(+)_Rep1_ATH1 | | |
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Tissue:
| whole root |
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in vivo Treatment:
| Plants were germinated and grown for 4 days on medium with high phosphate (2,5mM / (+)) and subsequently transferred for 20h to either medium with no phosphate (0mM / (-)) or to medium with high phosphate (control plants). |
---|
Additional Organism Information:
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Sample Description | Samples were derived from roots of 5 days old seedlings. |
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Other Information:
| |
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|
Protocols for BioSource 1 |
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Slide: Mueller_648-1_Col(+)_Rep1_ATH1 | | |
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Tissue:
| whole root |
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in vivo Treatment:
| Plants were germinated and grown for 4 days on medium with high phosphate (2,5mM) and subsequently transferred for 20h to medium with no phosphate (0mM). Control plants were transferred from high phosphate to high phosphate in order to ensure that changes in expression are not induced by mechanical stress. |
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Additional Organism Information:
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Sample Description | Samples were derived from roots of 5 days old seedlings. |
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Other Information:
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ecotype_habitat | |
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|
Protocols for BioSource 1 |
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