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Experiment: Group II-A WRKY transcription factors and early leaf senescence
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-398
In our laboratory we are interested in studying the functions of WRKY zinc finger type transcription factors. There are 74 members of this gene family in Arabidopsis. WRKY factors are key regulators of distinct plant defense responses and are involved in certain developmental programs e.g. trichome development and plant leaf senescence. We would like to determine the functions of a small sub-group (group II-a) of WRKY factors in response to bacterial infection. Our aim is to compare and contrast the gene expression profiles of 3-week-old wild type and WRKY T-DNA knockout mutants grown in a growth chamber under long day growth conditions and subsequently challenged for 6 hours with the virulent bacterial pathogen /Pseudomonas syringae/ DC3000.
About the ExperimenterName: | Dr. Bekir Uelker |
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Head of Lab Name: | Dr. Imre Somssich |
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Lab:
| Somsissich Lab |
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Institute:
| Max-Planck-Institute for Plant Breeding Research |
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Address: | Department of Plant Microbe Interactions Carl-von-Linne-Weg 10 Cologne
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Postcode:
| 50829 |
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Country:
| Germany |
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| Telephone Number:
| 49-221-5062-310 |
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Fax Number:
| 49-221-5062-353 |
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| URL for this person/group: | http://www2.mpiz-koeln.mpg.de/~somssich/wrky_webpage/wrky_family/wrky_family_index.html |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| genetic_modification_design |
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Number of Slides: | 7 |
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| Experimental Parameters:
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parameter | gene_knock_out |
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Quality Control Measures Taken:
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no-plants-pooled | 3 |
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References:
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Slides in this Experiment
Hybridisation Set: Uelker_genome_set
Slide: Ulker_2-1_WT-Col-0-L-MgCl2_Rep1_ATH1 | | |
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Tissue:
| Entire rosette |
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Diseased:
| Normal |
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Other Information:
| Control plants vacuum infiltrated with 5mM solution of MgCl2 |
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Protocols for BioSource 1 |
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Slide: Ulker_2-2_WRKY-KO-02-Pst-DC3000_Rep1_ATH1 | | |
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Tissue:
| Entire rosette |
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Other Information:
| Vacuum infiltration with /P. syringae / DC3000 (1x107 cfu/ml), harvested 6 hours after treatment |
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Protocols for BioSource 1 |
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Slide: Ulker_2-3_WRKY-KO-07-Pst-DC3000_Rep1_ATH1 | | |
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Tissue:
| Entire rosette |
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Other Information:
| Vacuum infiltration with /P. syringae / DC3000 (1x107 cfu/ml), harvested 6 hours after treatment |
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Protocols for BioSource 1 |
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Slide: Ulker_2-4_WRKY-KO-30-Pst-DC3000_Rep1_ATH1 | | |
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Tissue:
| Entire rosette |
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Other Information:
| Vacuum infiltration with /P. syringae / DC3000 (1x107 cfu/ml), harvested 6 hours after treatment |
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Protocols for BioSource 1 |
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Slide: Ulker_2-5_WRKY-KO-40-Pst-DC3000_Rep1_ATH1 | | |
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Tissue:
| Entire rosette |
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Other Information:
| Vacuum infiltration with /P. syringae / DC3000 (1x107 cfu/ml), harvested 6 hours after treatment |
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Protocols for BioSource 1 |
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Slide: Ulker_2-6_WRKY-KO-13-Pst-DC3000_Rep1_ATH1 | | |
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Tissue:
| Entire rosette |
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Other Information:
| Vacuum infiltration with /P. syringae / DC3000 (1x107 cfu/ml), harvested 6 hours after treatment |
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Protocols for BioSource 1 |
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Slide: Ulker_2-7_WT-Col-0-L-Pst-DC3000_Rep1_ATH1 | | |
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Tissue:
| Entire rosette |
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Other Information:
| Vacuum infiltration with /P. syringae / DC3000 (1x107 cfu/ml), harvested 6 hours after treatment |
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Protocols for BioSource 1 |
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