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Experiment: Microarray Analyses of Arabidopsis cgl1 (complex glycan 1) Mutants and Genetic Interaction with stt3a (staurosporin&temperature-sensitive 3a)
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-547
We compare two Arabidopsis thaliana cgl1 alleles and report on genetic interaction with stt3a in double mutants. STT3a encodes a subunit of oligo-saccharyl-trans-ferase that affects efficiency of N-glycan transfer to nascent secretory proteins in the ER; cgl1 mutants lack N-acetyl-gluco-saminyl-trans-ferase-I activity and are unable to form complex N-glycans in the Golgi apparatus. In cgl1 C6, a point mutation affects the 3'-splice site of intron 14, resulting in frame shifts; and in cgl1-T, intron 11 fails to splice due to insertion of a T-DNA copy. Introgression of stt3a-2 did not restore complex glycan formation in cgl1 C6 or cgl1-T (Frank et.al.,2008). In this microarray experiment, Seeds were grown on 1/4 MS media supplemented with 0.5% sucrose and 0.8% agar in a growth chamber under long-day photoperiod (16-hr light, 8-hr darkness) at 24 ± 2°C for 10 days. Then, seedlings were transferred into soil and grown three more weeks in a growth chamber at 25 ± 2ºC under long-day photoperiod at a light intensity of 40 μmol m-2s-1. Then, shoots from 5 plants were pooled and total RNAs were extracted according to Chomczynski (1993).
About the ExperimenterName: | Hisashi koiwa |
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Head of Lab Name: | Hisashi koiwa |
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Lab:
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Address: | Department of Horticultural Sciences, College of Agriculture and Life Science, Texas A & M University 202 Horticulture/Forest Science Building, , College Station, Texas, TX 77843-2133, UNITED STATES
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Country:
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| Telephone Number:
| +1 979-845-5282 |
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Fax Number:
| +1 979-845-0627 |
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are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| genetic_modification_design |
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Number of Slides: | 14 |
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| Experimental Parameters:
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parameter | gene_knock_out |
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Quality Control Measures Taken:
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References:
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Slides in this Experiment
Hybridisation Set: koiwa: Microarray Analyses of Arabidopsis cgl1 (complex glycan 1) Mutants and Genetic Interaction with stt3a (staurosporin&temperature-sensitive 3a)
Slide: koiwa_547-1_Col-0_Rep1_ATH1 | | |
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Tissue:
| shoot |
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Additional Organism Information:
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Sample Description | RNA was extracted from shoots pooled from 5 plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: koiwa_547-2_Col-0_Rep2_ATH1 | | |
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Tissue:
| shoot |
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Additional Organism Information:
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Sample Description | RNA was extracted from shoots pooled from 5 plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: koiwa_547-3_Col-0_Rep3_ATH1 | | |
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Tissue:
| shoot |
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Additional Organism Information:
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Sample Description | RNA was extracted from shoots pooled from 5 plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: koiwa_547-4_cgl-C6_Rep1_ATH1 | | |
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Tissue:
| shoot |
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Additional Organism Information:
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Sample Description | RNA was extracted from shoots pooled from 5 plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: koiwa_547-5_cgl-T_Rep1_ATH1 | | |
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Tissue:
| shoot |
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Additional Organism Information:
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Sample Description | RNA was extracted from shoots pooled from 5 plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: koiwa_547-6_cgl-T_Rep2_ATH1 | | |
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Tissue:
| shoot |
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Additional Organism Information:
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Sample Description | RNA was extracted from shoots pooled from 5 plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: koiwa_547-7_cgl-T_Rep3_ATH1 | | |
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Tissue:
| shoot |
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Additional Organism Information:
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Sample Description | RNA was extracted from shoots pooled from 5 plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: koiwa_548-1_stt3a_Rep1_ATH1 | | |
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Tissue:
| shoot |
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Additional Organism Information:
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Sample Description | RNA was extracted from shoots pooled from 5 plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: koiwa_548-2_stt3a_Rep2_ATH1 | | |
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Tissue:
| shoot |
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Additional Organism Information:
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Sample Description | RNA was extracted from shoots pooled from 5 plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: koiwa_548-3_stt3a_Rep3_ATH1 | | |
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Tissue:
| shoot |
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Additional Organism Information:
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Sample Description | RNA was extracted from shoots pooled from 5 plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: koiwa_548-4_stt3a-cglC6_Rep1_ATH1 | | |
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Tissue:
| shoot |
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Other Information:
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Protocols for BioSource 1 |
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Slide: koiwa_548-5_stt3a-cglT_Rep1_ATH1 | | |
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Tissue:
| shoot |
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Additional Organism Information:
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Sample Description | RNA was extracted from shoots pooled from 5 plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: koiwa_548-6_stt3a-cglT_Rep2_ATH1 | | |
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Tissue:
| shoot |
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Additional Organism Information:
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Sample Description | RNA was extracted from shoots pooled from 5 plants |
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Other Information:
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Protocols for BioSource 1 |
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Slide: koiwa_548-7_stt3a-cglT_Rep3_ATH1 | | |
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Tissue:
| shoot |
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Additional Organism Information:
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Sample Description | RNA was extracted from shoots pooled from 5 plants |
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Other Information:
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Protocols for BioSource 1 |
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