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Experiment: Effect of mycotoxin treatment on gene expression of wild-type and an altered sensitivity mutant
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-71
Fungal secondary metabolites can not only cause toxic effects in animals and humans, but also serve as virulence factors of the producing fungi for causing plant diseases.Thus, the severity of plant diseases associated with mycotoxins depend on the sensitivity towards the toxin. In previous experiments, we have evaluated the phytotoxic effect ofa mycotoxin on root growth of Arabidopsis wild-type and mutant seedlings. Mycotoxin treatment of a new conditional root expansion mutant partially restores the expansion phenotype (JE100; Werner et al., unpublished). AIM: This experiment aims to identify genes, in early and later phases after mycotoxin treatment in wild-type and mutant seedlings. EXPERIMENTAL PLAN: Eight Affymetrix chips are needed for this experiment. RNA preparation will be provided from wild-type, accession Columbia, and mutant seedlings after different time points of mycotoxin treatment. As control, separate seedlings will be treated with the same concentration of solvent (DMSO). Briefly, seeds will ben, seedlings will be transferred to liquid MS medium and shaken for another 3 days for acclimatization. Seedlings will be harvested after 2 and 24 hours of tk,...), the experiment will be repeated three times and RNA samples will be pooled. EXPECTED RESULTS: The experiment should identify genes differentially expressed: 1) between wild-type and mutant seedlings, 2) upon mycotoxin treatment in wild-type, 3) upon mycotoxin treatment of mutant seedlings and 4) upon solvent treatment.;
About the ExperimenterName: | Dipl.Ing. Ulrike Werner |
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Head of Lab Name: | Dr. Marie-Theres Hauser |
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Lab:
| Institute of Applied Genetics and Cell Biology |
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Institute:
| BOKU |
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Address: | Center of Applied Genetics University of Agricultural Sciences Vienna Muthgasse 18 Vienna
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Postcode:
| 1190 |
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Country:
| Austria |
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| Telephone Number:
| +43-1-36006-6371 |
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Fax Number:
| +43-1-36006-6392 |
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| URL for this person/group: | http://www.boku.ac.at/zag/AG_hauser.htm |
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are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| genetic_modification_design; compound_treatment_design; |
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Number of Slides: | 8 |
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| Experimental Parameters:
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Parameter | compound_based_treatment |
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parameter | gene_knock_out |
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parameter | organism |
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parameter | age |
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Quality Control Measures Taken:
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no-plants-pooled | 10 - 20 seedlings |
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References:
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| Other Information:
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ArrayExpressAccession | E-NASC-52 |
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Slides in this Experiment
Hybridisation Set: Werner_genome
Slide: Werner_1-1_wildtype-2hr-control(c2s)_Rep1_ATH1 | | |
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Tissue:
| 15 DAG seedlings |
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Other Information:
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Treatment | After three days of acclimatization plants were treated with solvent (10% DMSO in 0.01 N KOH, 50µl in 10ml medium)for two hours as Mock control. |
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Protocols for BioSource 1 |
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Slide: Werner_1-2_wildtype-24hr-control(c4s)_Rep1_ATH1 | | |
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Tissue:
| 15 DAG seedlings |
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Diseased:
| Normal |
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Other Information:
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Treatment | After three days of acclimatization plants were treated with solvent (10% DMSO in 0.01 N KOH, 50µl in 10ml medium)for 24 hours as Mock control. |
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Protocols for BioSource 1 |
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Slide: Werner_1-3_mutant-2hr-control(j2s)_Rep1_ATH1 | | |
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Tissue:
| 15 DAG seedlings |
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Diseased:
| Normal |
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Other Information:
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Treatment | After three days of acclimatization plants were treated with solvent(10% DMSO in 0.01 N KOH, 50µl in 10ml medium)for two hours as Mock control. |
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Protocols for BioSource 1 |
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Slide: Werner_1-4_mutant-24hr-control(j4s)_Rep1_ATH1 | | |
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Tissue:
| 15 DAG seedlings |
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Diseased:
| Normal |
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Other Information:
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Treatment | After three days of acclimatization plants were treated with solvent(10% DMSO in 0.01 N KOH, 50µl in 10ml medium)for 24 hours as Mock control. |
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Protocols for BioSource 1 |
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Slide: Werner_1-5_wildtype-2hr-zearalenone(c2t)_Rep1_ATH1 | | |
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Tissue:
| 15 DAG seedlings |
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Diseased:
| Normal |
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in vitro Treatment:
| 50µM of zearalenone |
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Other Information:
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Treatment | After three days of acclimatization plants were treated with 50µM of zearalenone (solved in 10% DMSO in 0.01 N KOH, 50µl in 10ml medium)for two hours. |
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Protocols for BioSource 1 |
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Slide: Werner_1-6_wildtype-24hr-zearalenone(c4t)_Rep1_ATH1 | | |
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Tissue:
| 15 DAG seedlings |
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Diseased:
| Normal |
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in vitro Treatment:
| 50µM of zearalenone |
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Other Information:
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Treatment | After three days of acclimatization plants were treated with 50µM of zearalenone (solved in 10% DMSO in 0.01 N KOH, 50µl in 10ml medium)for 24 hours. |
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Protocols for BioSource 1 |
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Slide: Werner_1-7_mutant-2hr-zearalenone(j2t)_Rep1_ATH1 | | |
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Tissue:
| 15 DAG seedlings |
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Diseased:
| Normal |
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Other Information:
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Treatment | After three days of acclimatization plants were treated with 50µM zearalenone (solved in 10% DMSO in 0.01 N KOH, 50µl in 10ml medium) for two hours. |
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Protocols for BioSource 1 |
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Slide: Werner_1-8_mutant-24hr-zearalenone(j4t)_Rep1_ATH1 | | |
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Tissue:
| 15 DAG seedlings |
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Diseased:
| Normal |
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Other Information:
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Treatment | After three days of acclimatization plants were treated with 50µM zearalenone (solved in 10% DMSO in 0.01 N KOH, 50µl in 10ml medium) for 24 hours. |
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Protocols for BioSource 1 |
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Problems? Comments? Suggestions? Contact the Affymetrix Team