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Experiment: Compatibility to Downy Mildew (Hyaloperonospora arabidopsidis) infection
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-585
The analysis of physiological and molecular determinants accounting for successful infection by pathogenic oomycetes has become a topic of prime scientific interest during the last years. The hunt is now on for pathogen effectors subverting the host cell, and for the plant compatibility functions manipulated by these effectors. An understanding of the molecular mechanisms underlying successful infection should make it possible to develop new crop protection strategies based on interference with compatibility to prevent disease. We identified several Arabidopsis genes that account for full susceptibility to the Downy Mildew pathogen, Hyaloperonospora arabidopsidis. A common denominator is that mutants, in which these genes were knocked-out, are more resistant (= less susceptible) to infection. Here, we aim at analyzing the metabolic pathways that are responsible for the lowered susceptibility phenotype of three mutant lines, which are deficient for a microtubule-associated protein (MAP65-3), a leucine-rich repeat receptor-like kinase (PSKR1), and a glycosyltransferase.The Wassilewskija (Ws-4) wild-typ and the mutant lines dyc283, eyw110, and egy19 were from the T-DNA insertion collection at INRA, Versailles, France. Seeds were sown to high density on a soil/sand mixture in 6 cm x 6 cm pots, stratified for 3 days at 4 °C, and then grown under a 16 h photoperiod in a growth chamber at 20 °C. For control treatment and infection, 8-day-old plants were spray-inoculated to saturation with either water, or a H. arabidopsidis isolate Emwa1 spore suspension at 40,000 spores/ml, respectively. Plants were kept in a growth cabinet at 16°C for 3 d with a 16 h photoperiod, before the aerial parts including hypocotyls, fully expanded cotyledons, and leaf primordia were pooled from ~500 plantlets for RNA extraction.
About the ExperimenterName: | Dr Harald Keller |
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Head of Lab Name: | Dr Harald Keller |
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Lab:
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Institute:
| Equipe Interactions Plantes-Oomycetes, UMR Interactions Biotiques et Santale, Institut National de la Recherche Agronomique (INRA)
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Address: | 400, Route des Chappes. Boïte Postale 167 , Sophia-Antipolis Cedex, Antibes, Alpes-Maritimes
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Postcode:
| 06903 |
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Country:
| FRANCE
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| Telephone Number:
| 33(0)4 92 38 65 94 |
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Fax Number:
| 33(0)4 92 38 65 87 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| genetic_modification_design |
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Number of Slides: | 16 |
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| Experimental Parameters:
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parameter | infect |
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parameter | gene_knock_out |
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parameter | |
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Quality Control Measures Taken:
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Keller: Compatibility to Downy Mildew (Hyaloperonospora arabidopsidis) infection
Slide: Keller_585-1_Ws_Water_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod. |
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Additional Organism Information:
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Sample Description | Aerial parts, hypocotyls, cotyledons and leaf primordia |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Keller_585-2_Ws_Water_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod. |
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Additional Organism Information:
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Sample Description | Aerial parts, hypocotyls, cotyledons and leaf primordia |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Keller_585-3_Ws_Ha_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod. |
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Additional Organism Information:
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Sample Description | Aerial parts, hypocotyls, cotyledons and leaf primordia |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Keller_585-4_Ws_Ha_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod. |
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Additional Organism Information:
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Sample Description | Aerial parts, hypocotyls, cotyledons and leaf primordia |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Keller_585-5_dyc283_Water_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod. |
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Additional Organism Information:
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Sample Description | Aerial parts, hypocotyls, cotyledons and leaf primordia |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Keller_585-6_dyc283_Water_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod. |
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Additional Organism Information:
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Sample Description | Aerial parts, hypocotyls, cotyledons and leaf primordia |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Keller_585-7_dyc283_Ha_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod. |
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Additional Organism Information:
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Sample Description | Aerial parts, hypocotyls, cotyledons and leaf primordia |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Keller_585-8_dyc283_Ha_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod. |
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Additional Organism Information:
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Sample Description | Aerial parts, hypocotyls, cotyledons and leaf primordia |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Keller_585-9_eyw110_Water_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod. |
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Additional Organism Information:
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Sample Description | Aerial parts, hypocotyls, cotyledons and leaf primordia |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Keller_585-10_eyw110_Water_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod. |
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Additional Organism Information:
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Sample Description | Aerial parts, hypocotyls, cotyledons and leaf primordia |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Keller_585-11_eyw110_Ha_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod. |
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Additional Organism Information:
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Sample Description | Aerial parts, hypocotyls, cotyledons and leaf primordia |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Keller_585-12_eyw110_Ha_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod. |
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Additional Organism Information:
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Sample Description | Aerial parts, hypocotyls, cotyledons and leaf primordia |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Keller_585-13_egy19_Water_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod. |
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Additional Organism Information:
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Sample Description | Aerial parts, hypocotyls, cotyledons and leaf primordia |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Keller_585-14_egy19_Water_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod. |
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Additional Organism Information:
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Sample Description | Aerial parts, hypocotyls, cotyledons and leaf primordia |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Keller_585-15_egy19_Ha_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod. |
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Additional Organism Information:
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Sample Description | Aerial parts, hypocotyls, cotyledons and leaf primordia |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Keller_585-16_egy19_Ha_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod. |
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Additional Organism Information:
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Sample Description | Aerial parts, hypocotyls, cotyledons and leaf primordia |
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Other Information:
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|
Protocols for BioSource 1 |
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Problems? Comments? Suggestions? Contact the Affymetrix Team