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Experiment: Fenclorim safening of Arabidopsis
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-494
Wildtype arabidopsis thaliana Col-0 root cultures, were treated with fenclorim or 4-chloro-6-methyl-2-phenylpyrimidine dissolved in acetone to achieve a final concentration of 100uM. The final acetone concentration of 0.1% was replicated in control root cultures. Samples were taken at four and twenty-four hours post addition in biological triplicate. Root cultures were routinely maintained at 25oC in the dark.
About the ExperimenterName: | Dr Mark Skipsey |
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Head of Lab Name: | Prof Robert Edwards |
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Lab:
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Address: | Durham University School of Biological and Biomedical Sciences University Science Laboratories South Road Durham
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Postcode:
| DH1 3LE |
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Country:
| UK |
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| Telephone Number:
| 01913342143 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| time_series_design; compound_treatment_design |
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Number of Slides: | 18 |
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| Experimental Parameters:
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parameter | timepoint |
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parameter | compound_based_treatment |
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Quality Control Measures Taken:
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Skipsey: Fenclorim safening of Arabidopsis_genome
Slide: Skipsey_1-8_Fenclorim_4hr_Rep2_ATH1 | | |
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Tissue:
| root |
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in vivo Treatment:
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Treatment | The three treatments employed in this study were addition of acetone as a control treatment as this solvent carrier was used to dissolve the compounds in the other treatments. Secondly the safener compound fenclorim was added to cultures. Treatment compounds were added to Arabidopsis root cultures for 4 h and 24 h periods prior to RNA extraction. |
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Other Information:
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RNAQC | No |
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RNAReceived | No |
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Protocols for BioSource 1 |
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Slide: Skipsey_1-7_Fenclorim_4hr_Rep1_ATH1 | | |
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Tissue:
| root |
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in vivo Treatment:
| |
---|
Treatment | The three treatments employed in this study were addition of acetone as a control treatment as this solvent carrier was used to dissolve the compounds in the other treatments. Secondly the safener compound fenclorim was added to cultures. Treatment compounds were added to Arabidopsis root cultures for 4 h and 24 h periods prior to RNA extraction. |
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Other Information:
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RNAQC | No |
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RNAReceived | No |
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Protocols for BioSource 1 |
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Slide: Skipsey_1-6_Acetone_24hr_Rep3_ATH1 | | |
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Tissue:
| root |
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in vivo Treatment:
| |
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Treatment | The inducing chemical treatments were prepared as 100 mM stocks in acetone and added to the medium as a 1:1000 dilution. Control treatments consisted of 0.1% v/v acetone. Treatment compounds were added to Arabidopsis root cultures for 4 h and 24 h periods prior to RNA extraction. |
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Other Information:
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RNAQC | No |
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RNAReceived | No |
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Protocols for BioSource 1 |
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Slide: Skipsey_1-5_Acetone_24hr_Rep2_ATH1 | | |
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Tissue:
| root |
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in vivo Treatment:
| |
---|
Treatment | The inducing chemical treatments were prepared as 100 mM stocks in acetone and added to the medium as a 1:1000 dilution. Control treatments consisted of 0.1% v/v acetone. Treatment compounds were added to Arabidopsis root cultures for 4 h and 24 h periods prior to RNA extraction. |
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Other Information:
| |
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RNAQC | No |
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RNAReceived | No |
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Protocols for BioSource 1 |
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Slide: Skipsey_1-4_Acetone_24hr_Rep1_ATH1 | | |
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Tissue:
| root |
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in vivo Treatment:
| |
---|
Treatment | The inducing chemical treatments were prepared as 100 mM stocks in acetone and added to the medium as a 1:1000 dilution. Control treatments consisted of 0.1% v/v acetone. Treatment compounds were added to Arabidopsis root cultures for 4 h and 24 h periods prior to RNA extraction. |
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Other Information:
| |
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RNAQC | No |
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RNAReceived | No |
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Protocols for BioSource 1 |
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Slide: Skipsey_1-3_Acetone_4hr_Rep3_ATH1 | | |
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Tissue:
| root |
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in vivo Treatment:
| |
---|
Treatment | The inducing chemical treatments were prepared as 100 mM stocks in acetone and added to the medium as a 1:1000 dilution. Control treatments consisted of 0.1% v/v acetone. Treatment compounds were added to Arabidopsis root cultures for 4 h and 24 h periods prior to RNA extraction. |
---|
Other Information:
| |
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RNAQC | No |
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RNAReceived | No |
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|
Protocols for BioSource 1 |
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Slide: Skipsey_1-2_Acetone_4hr_Rep2_ATH1 | | |
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Tissue:
| root |
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in vivo Treatment:
| |
---|
Treatment | The inducing chemical treatments were prepared as 100 mM stocks in acetone and added to the medium as a 1:1000 dilution. Control treatments consisted of 0.1% v/v acetone. Treatment compounds were added to Arabidopsis root cultures for 4 h and 24 h periods prior to RNA extraction. |
---|
Other Information:
| |
---|
RNAQC | No |
---|
RNAReceived | No |
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|
Protocols for BioSource 1 |
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Slide: Skipsey_1-1_Acetone_4hr_Rep1_ATH1 | | |
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|
Tissue:
| root |
---|
in vivo Treatment:
| |
---|
Treatment | The inducing chemical treatments were prepared as 100 mM stocks in acetone and added to the medium as a 1:1000 dilution. Control treatments consisted of 0.1% v/v acetone. Treatment compounds were added to Arabidopsis root cultures for 4 h and 24 h periods prior to RNA extraction. |
---|
Other Information:
| |
---|
RNAQC | No |
---|
RNAReceived | No |
---|
|
Protocols for BioSource 1 |
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Slide: Skipsey_1-9_Fenclorim_4hr_Rep3_ATH1 | | |
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|
Tissue:
| root |
---|
in vivo Treatment:
| |
---|
Treatment | The three treatments employed in this study were addition of acetone as a control treatment as this solvent carrier was used to dissolve the compounds in the other treatments. Secondly the safener compound fenclorim was added to cultures. Treatment compounds were added to Arabidopsis root cultures for 4 h and 24 h periods prior to RNA extraction. |
---|
Other Information:
| |
---|
RNAQC | No |
---|
RNAReceived | No |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Skipsey_1-10_Fenclorim_24hr_Rep1_ATH1 | | |
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|
Tissue:
| root |
---|
in vivo Treatment:
| |
---|
Treatment | The three treatments employed in this study were addition of acetone as a control treatment as this solvent carrier was used to dissolve the compounds in the other treatments. Secondly the safener compound fenclorim was added to cultures. Treatment compounds were added to Arabidopsis root cultures for 4 h and 24 h periods prior to RNA extraction. |
---|
Other Information:
| |
---|
RNAQC | No |
---|
RNAReceived | No |
---|
|
Protocols for BioSource 1 |
|
|
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Slide: Skipsey_1-11_Fenclorim_24hr_Rep2_ATH1 | | |
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Tissue:
| root |
---|
in vivo Treatment:
| |
---|
Treatment | The three treatments employed in this study were addition of acetone as a control treatment as this solvent carrier was used to dissolve the compounds in the other treatments. Secondly the safener compound fenclorim was added to cultures. Treatment compounds were added to Arabidopsis root cultures for 4 h and 24 h periods prior to RNA extraction. |
---|
Other Information:
| |
---|
RNAQC | No |
---|
RNAReceived | No |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Skipsey_1-12_Fenclorim_24hr_Rep3_ATH1 | | |
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|
Tissue:
| root |
---|
in vivo Treatment:
| |
---|
Treatment | The three treatments employed in this study were addition of acetone as a control treatment as this solvent carrier was used to dissolve the compounds in the other treatments. Secondly the safener compound fenclorim was added to cultures. Treatment compounds were added to Arabidopsis root cultures for 4 h and 24 h periods prior to RNA extraction. |
---|
Other Information:
| |
---|
RNAQC | No |
---|
RNAReceived | No |
---|
|
Protocols for BioSource 1 |
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Slide: Skipsey_1-13_CMP_4hr_Rep1_ATH1 | | |
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Tissue:
| root |
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in vivo Treatment:
| |
---|
Treatment | The three treatments employed in this study were addition of acetone as a control treatment as this solvent carrier was used to dissolve the compounds in the other treatments. Finally 4-chloro-6-methyl-2-phenylpyrimidine (CMP) was added to cultures. Treatment compounds were added to Arabidopsis root cultures for 4 h and 24 h periods prior to RNA extraction. |
---|
Other Information:
| |
---|
RNAQC | No |
---|
RNAReceived | No |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Skipsey_1-14_CMP_4hr_Rep2_ATH1 | | |
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|
Tissue:
| root |
---|
in vivo Treatment:
| |
---|
Treatment | The three treatments employed in this study were addition of acetone as a control treatment as this solvent carrier was used to dissolve the compounds in the other treatments. Finally 4-chloro-6-methyl-2-phenylpyrimidine (CMP) was added to cultures. Treatment compounds were added to Arabidopsis root cultures for 4 h and 24 h periods prior to RNA extraction. |
---|
Other Information:
| |
---|
RNAQC | No |
---|
RNAReceived | No |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Skipsey_1-15_CMP_4hr_Rep3_ATH1 | | |
|
|
|
Tissue:
| root |
---|
in vivo Treatment:
| |
---|
Treatment | The three treatments employed in this study were addition of acetone as a control treatment as this solvent carrier was used to dissolve the compounds in the other treatments. Finally 4-chloro-6-methyl-2-phenylpyrimidine (CMP) was added to cultures. Treatment compounds were added to Arabidopsis root cultures for 4 h and 24 h periods prior to RNA extraction. |
---|
Other Information:
| |
---|
RNAQC | No |
---|
RNAReceived | No |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Skipsey_1-16_CMP_24hr_Rep1_ATH1 | | |
|
|
|
Tissue:
| root |
---|
in vivo Treatment:
| |
---|
Treatment | The three treatments employed in this study were addition of acetone as a control treatment as this solvent carrier was used to dissolve the compounds in the other treatments. Finally 4-chloro-6-methyl-2-phenylpyrimidine (CMP) was added to cultures. Treatment compounds were added to Arabidopsis root cultures for 4 h and 24 h periods prior to RNA extraction. |
---|
Other Information:
| |
---|
RNAQC | No |
---|
RNAReceived | No |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Skipsey_1-17_CMP_24hr_Rep2_ATH1 | | |
|
|
|
Tissue:
| root |
---|
in vivo Treatment:
| |
---|
Treatment | The three treatments employed in this study were addition of acetone as a control treatment as this solvent carrier was used to dissolve the compounds in the other treatments. Finally 4-chloro-6-methyl-2-phenylpyrimidine (CMP) was added to cultures. Treatment compounds were added to Arabidopsis root cultures for 4 h and 24 h periods prior to RNA extraction. |
---|
Other Information:
| |
---|
RNAQC | No |
---|
RNAReceived | No |
---|
|
Protocols for BioSource 1 |
|
|
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Slide: Skipsey_1-18_CMP_24hr_Rep3_ATH1 | | |
|
|
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Tissue:
| root |
---|
in vivo Treatment:
| |
---|
Treatment | The three treatments employed in this study were addition of acetone as a control treatment as this solvent carrier was used to dissolve the compounds in the other treatments. Finally 4-chloro-6-methyl-2-phenylpyrimidine (CMP) was added to cultures. Treatment compounds were added to Arabidopsis root cultures for 4 h and 24 h periods prior to RNA extraction. |
---|
Other Information:
| |
---|
RNAQC | No |
---|
RNAReceived | No |
---|
|
Protocols for BioSource 1 |
|
|
|
Problems? Comments? Suggestions? Contact the Affymetrix Team