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Experiment: DUO1 ectopic expression
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-439
The experiment is designed to examine changes in gene expression upon ectopic expression of the germ line transcription factor DUO1 in Arabidopsis. Plants were transformed with the vector pMDC7DUO1, allowing induction of DUO1 by treatment with estradiol. T2 seedlings were selected on agar plates containing hygromycin for 12 days before seedlings were transferred to plates containing either 2 µM 17ß-estradiol (induced), or control plates containing DMSO (non-induced). Plants were grown for 6, 12 or 24 hours before harvesting. RNA was extracted using the RNAeasy kit from Qiagen. Each sample consists of 20 seedlings combined and all treatments have been conducted in triplicate.
About the ExperimenterName: | Dr Lynette Brownfield |
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Head of Lab Name: | Prof Twell David |
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Lab:
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Address: | Department of Biology University of Leicester University Rd Leicester
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Postcode:
| LE1 7RH |
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Country:
| UK |
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| Telephone Number:
| 01162522266 |
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Fax Number:
| 01162523330 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| compound_treatment_design; time_series_design; |
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Number of Slides: | 18 |
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| Experimental Parameters:
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parameter | compound_based_treatment |
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parameter | timepoint |
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Quality Control Measures Taken:
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Brownfield: DUO1 ectopic expression_genome
Slide: Brownfield_1-1_6hr-not-induced_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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Treatment protocol | Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing DMSO (2ul in 100ml) and incubated under constant light at 22°C further 6 h. |
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Additional Organism Information:
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sampledescription | Seedling with 4 true leaves plus cotyledons, root approximately 2 cm |
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Other Information:
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Allele | At3g60460 |
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Protocols for BioSource 1 |
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Slide: Brownfield_1-2_6hr-not-induced_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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Treatment protocol | Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing DMSO (2ul in 100ml) and incubated under constant light at 22°C further 6 h. |
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Additional Organism Information:
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sampledescription | Seedling with 4 true leaves plus cotyledons, root approximately 2 cm |
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Other Information:
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Allele | At3g60460 |
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Protocols for BioSource 1 |
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Slide: Brownfield_1-3_6hr-not-induced_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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Treatment protocol | Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing DMSO (2ul in 100ml) and incubated under constant light at 22°C further 6 h. |
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Additional Organism Information:
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sampledescription | Seedling with 4 true leaves plus cotyledons, root approximately 2 cm |
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Other Information:
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Allele | At3g60460 |
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Protocols for BioSource 1 |
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Slide: Brownfield_1-4_6hr-induced_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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Treatment protocol | Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing 2 um estradiol and incubated under constant light at 22°C further 6 h. |
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Additional Organism Information:
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sampledescription | Seedling with 4 true leaves plus cotyledons, root approximately 2 cm. |
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Other Information:
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Allele | At3g60460 |
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Protocols for BioSource 1 |
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Slide: Brownfield_1-5_6hr-induced_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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Treatment protocol | Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing 2 um estradiol and incubated under constant light at 22°C further 6 h. |
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Additional Organism Information:
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sampledescription | Seedling with 4 true leaves plus cotyledons, root approximately 2 cm. |
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Other Information:
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Allele | At3g60460 |
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Protocols for BioSource 1 |
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Slide: Brownfield_1-6_6hr-induced_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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Treatment protocol | Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing 2 um estradiol and incubated under constant light at 22°C further 6 h. |
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Additional Organism Information:
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sampledescription | Seedling with 4 true leaves plus cotyledons, root approximately 2 cm. |
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Other Information:
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Allele | At3g60460 |
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Protocols for BioSource 1 |
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Slide: Brownfield_1-7_12hr-not-induced_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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Treatment protocol | Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing DMSO (2 ul in 100 ml) and incubated under constant light at 22°C further 12 h. |
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Additional Organism Information:
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sampledescription | Seedling with 4 true leaves plus cotyledons, root approximately 2 cm. |
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Other Information:
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Allele | At3g60460 |
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Protocols for BioSource 1 |
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Slide: Brownfield_1-8_12hr-not-induced_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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Treatment protocol | Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing DMSO (2 ul in 100 ml) and incubated under constant light at 22°C further 12 h. |
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Additional Organism Information:
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sampledescription | Seedling with 4 true leaves plus cotyledons, root approximately 2 cm. |
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Other Information:
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Allele | At3g60460 |
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Protocols for BioSource 1 |
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Slide: Brownfield_1-9_12hr-not-induced_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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Treatment protocol | Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing DMSO (2 ul in 100 ml) and incubated under constant light at 22°C further 12 h. |
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Additional Organism Information:
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sampledescription | Seedling with 4 true leaves plus cotyledons, root approximately 2 cm. |
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Other Information:
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Allele | At3g60460 |
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Protocols for BioSource 1 |
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Slide: Brownfield_1-10_12hr-induced_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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Treatment protocol | Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing 2 uM estradiol and incubated under constant light at 22°C further 12 h. |
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Additional Organism Information:
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sampledescription | Seedling with 4 true leaves plus cotyledons, root approximately 2 cm. |
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Other Information:
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Allele | At3g60460 |
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Protocols for BioSource 1 |
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Slide: Brownfield_1-11_12hr-induced_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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Treatment protocol | Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing 2 uM estradiol and incubated under constant light at 22°C further 12 h. |
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Additional Organism Information:
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sampledescription | Seedling with 4 true leaves plus cotyledons, root approximately 2 cm. |
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Other Information:
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Allele | At3g60460 |
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Protocols for BioSource 1 |
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Slide: Brownfield_1-12_12hr-induced_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| |
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Treatment protocol | Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing 2 uM estradiol and incubated under constant light at 22°C further 12 h. |
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Additional Organism Information:
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sampledescription | Seedling with 4 true leaves plus cotyledons, root approximately 2 cm. |
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Other Information:
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Allele | At3g60460 |
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Protocols for BioSource 1 |
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Slide: Brownfield_1-13_24hr-not-induced_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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Treatment protocol | Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing DMSO (2 ul in 100 ml) and incubated under constant light at 22°C further 24 h. |
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Additional Organism Information:
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sampledescription | Seedling with 4 true leaves plus cotyledons, root approximately 2 cm. |
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Other Information:
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Allele | At3g60460 |
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Protocols for BioSource 1 |
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Slide: Brownfield_1-14_24hr-not-induced_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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Treatment protocol | Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing DMSO (2 ul in 100 ml) and incubated under constant light at 22°C further 24 h. |
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Additional Organism Information:
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sampledescription | Seedling with 4 true leaves plus cotyledons, root approximately 2 cm. |
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Other Information:
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Allele | At3g60460 |
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Protocols for BioSource 1 |
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Slide: Brownfield_1-15_24hr-not-induced_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| |
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Treatment protocol | Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing DMSO (2 ul in 100 ml) and incubated under constant light at 22°C further 24 h. |
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Additional Organism Information:
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sampledescription | Seedling with 4 true leaves plus cotyledons, root approximately 2 cm. |
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Other Information:
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Allele | At3g60460 |
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Protocols for BioSource 1 |
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Slide: Brownfield_1-16_24hr-induced_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| |
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Treatment protocol | Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing 2 uM estradiol and incubated under constant light at 22°C further 24 h. |
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Additional Organism Information:
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sampledescription | Seedling with 4 true leaves plus cotyledons, root approximately 2 cm. |
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Other Information:
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Allele | At3g60460 |
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Protocols for BioSource 1 |
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Slide: Brownfield_1-17_24hr-induced_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| |
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Treatment protocol | Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing 2 uM estradiol and incubated under constant light at 22°C further 24 h. |
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Additional Organism Information:
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sampledescription | Seedling with 4 true leaves plus cotyledons, root approximately 2 cm. |
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Other Information:
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Allele | At3g60460 |
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Protocols for BioSource 1 |
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Slide: Brownfield_1-18_24hr-induced_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
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Treatment protocol | Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing 2 uM estradiol and incubated under constant light at 22°C further 24 h. |
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Additional Organism Information:
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sampledescription | Seedling with 4 true leaves plus cotyledons, root approximately 2 cm. |
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Other Information:
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Allele | At3g60460 |
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Protocols for BioSource 1 |
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Problems? Comments? Suggestions? Contact the Affymetrix Team