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Experiment: cpl4 microarray analysis
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-658
An Arabidopsis thaliana gene called RNA polymerase II CTD phosphatase-like 4 (CPL4) is orthologous to fungal and metazoan FCP1, and is necessary for normal plant growth and development. RNAi suppression of CPL4 expression results in growth defects that range from transient retardation of seedling development to lethality prior to maturation. The aim of this experiment is to identify genes differentially expressed in CPL4 RNAi plants. For this purpose, seeds were surface sterilized by 70% ethanol and washed with distilled water for several times. Seeds were grown on ¼ strength MS basal media containing 0.5% sucrose, solidified with 0.8% plant cell tissue grade agar. Approximately 200 seeds were sown on each petri dish and stratified for 4 days at 4°C. Then, they were transferred to a growth chamber providing 16h of light and 8h of darkness at 25°C. After 10 days of growth at these conditions, total RNA was extracted from 100mg of seedlings by using TRIzol (Invitrogen, Cat. No. 15596-018) method according to the manufacturer’s procedure. Then, total RNA samples were treated with DNase I (Qiagen, Cat No. 79254) at 37°C for 1 hours and cleaned using Qiagen minicolumns.
About the ExperimenterName: | Hisashi koiwa |
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Head of Lab Name: | Hisashi koiwa |
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Lab:
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Address: | Department of Horticultural Sciences, College of Agriculture and Life Science, Texas A & M University 202 Horticulture/Forest Science Building, , College Station, Texas, TX 77843-2133, UNITED STATES
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Postcode:
| TX 77843-2133 |
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Country:
| UNITED STATES |
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| Telephone Number:
| +1 979-845-5282 |
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Fax Number:
| +1 979-845-0627 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| genetic_modification |
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Number of Slides: | 6 |
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| Experimental Parameters:
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parameter | gene_knock_out |
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Quality Control Measures Taken:
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References:
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Slides in this Experiment
Hybridisation Set: koiwa: cpl4 microarray analysis
Slide: koiwa_658-1_CPL4-RNAi_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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Other Information:
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Protocols for BioSource 1 |
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Slide: koiwa_658-2_CPL4-RNAi_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Other Information:
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Protocols for BioSource 1 |
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Slide: koiwa_658-3_CPL4-RNAi_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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Other Information:
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Protocols for BioSource 1 |
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Slide: koiwa_658-4_gl1-RNAi_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | 10 day old whole seedlings were used for RNA extraction |
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Other Information:
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Protocols for BioSource 1 |
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Slide: koiwa_658-5_gl1-RNAi_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | 10 day old whole seedlings were used for RNA extraction |
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Other Information:
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Protocols for BioSource 1 |
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Slide: koiwa_658-6_gl1-RNAi_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | 10 day old whole seedlings were used for RNA extraction |
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Other Information:
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Protocols for BioSource 1 |
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