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Experiment: Comparison of gonst1 mutant callus with WT
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-704
The aim of this experiment is to compare two independent knockout mutations in the gonst1 gene (At2g13650) with WT. As the plants do not grow on soil, and poorly on agar, we have made callus cell culture lines, from which this RNA is prepared. GONST1 is thought to be a Golgi-localsied GDP-sugar transporter (Baldwin et al., 2001; Handford et al., 2004), although it's precise role in planta is unknown.Baldwin, TC, Handford, MG, Yuseff, I, Orellana, O and P Dupree. (2001) Identification and Characterization of GONST1, a Golgi-Localized GDP- Mannose Transporter in Arabidopsis. The Plant Cell, 13, 2283ÂÂ-2295Handford MG, Sicilia F, Brandizzi F, Chung JH and P Dupree. (2004) Arabidopsis thaliana expresses multiple Golgi-localised nucleotide-sugar transporters related to GONST1. Molecular and General Genomics, 272, 397-410
About the ExperimenterName: | Dr Jenny Mortimer |
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Head of Lab Name: | Dr Paul Dupree |
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Lab:
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Address: | Hopkins Building Department of Biochemistry University of Cambridge Building O, Downing Site Cambridge
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Postcode:
| CB1 3AW |
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Country:
| UK |
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| Telephone Number:
| 01223 333686 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| genetic_modification |
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Number of Slides: | 6 |
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| Experimental Parameters:
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parameter | genetic_modification |
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Quality Control Measures Taken:
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References:
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Reference | Baldwin, TC, Handford, MG, Yuseff, I, Orellana, O and P Dupree. (2001) |
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Reference | Baldwin et al., 2001; Handford et al., 2004 |
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Reference | The Plant Cell, 13, 2283Â-2295 Handford MG, Sicilia F, Brandizzi F, Chung JH and P Dupree. (2004) |
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Reference | Molecular and General Genomics, 272, 397-410 |
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Slides in this Experiment
Hybridisation Set: Mortimer: Comparison of gonst1 mutant callus with WT
Slide: Mortimer_704-1_Col0-callus_Rep1_ATH1 | | |
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Tissue:
| Callus cells |
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Additional Organism Information:
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Sample Description | Callus cells |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Mortimer_704-2_Col0-callus_Rep2_ATH1 | | |
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Tissue:
| Callus cells |
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Additional Organism Information:
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Sample Description | Callus cells |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Mortimer_704-3_gonst1-2-callus_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Callus suspension cell culture |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Mortimer_704-4_gonst1-2-callus_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Callus suspension cell culture |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Mortimer_704-5_gonst1-3-callus_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Callus suspension cell culture |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Mortimer_704-6_gonst1-3-callus_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | Callus suspension cell culture |
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Other Information:
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Protocols for BioSource 1 |
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