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Experiment: Cell-specific nitrogen responses in the Arabidopsis root
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-490
Overview:
The organs of multicellular species are comprised of cell types that must function together to perform specific tasks. One critical organ function is responding to internal or external change but little is known about how responses are tailored to specific cell types or coordinated among them on a global level. Here we use cellular profiling of five Arabidopsis root cell types in response to a limiting resource, nitrogen, to uncover a vast and predominantly cell-specific response that was largely undetectable using traditional methods. These methods reveal a new class of cell-specific nitrogen responses. As a proof-of-principle, we dissected one cell-specific response circuit that mediates nitrogen-induced changes in root branching from pericycle cells. Thus, cellular response profiling links gene modules to discrete functions in specific cell types.
Aim:
We used microarrays to detail the global programme of gene expression involved in the plants response to nitrogen in the root and identified distinct classes of up- and down-regulated genes during this process. We further distinguished the nitrogen-responsive genes into (i) five cell types, and (ii) inorganic nitrogen (nitrate) or organic nitrogen (amino acid) responsive genes.
Experimental setup:
The whole experiment was carried out in at least triplicate. The nitrogen response in 5 FACS-sorted root cell types (LRC, lateral root cap; EpiC, epidermis and cortex; EndoP, endodermis and pericycle; Peri, pericycle; Stele, stele), protoplasts and Col-0 whole roots was assayed using microarrays. 5mM KNO3 was used as a nitrogen treatment for 2 hours. 5mM KCl was used as a control treatment for the same length of time. 1mM MSX in combination with 5mM KNO3 (or with 5mM KCl as a control) was used to block the assimilation of KNO3, and 5mM Gln added to restore it in order to separate KNO3 effects from assimilated-nitrogen effects. 5mM KNO3 was either maintained in the protoplast-generating solution (continuous treat) or not (transitory treat) to test how to best preserve the cellular nitrogen-response during the 1 hr protoplast generating procedure. As controls we used protoplasts, whole roots frozen directly after the 2hr treatment or 3.5hr treatment, or whole roots incubated in protoplast-generating solution (minus enzymes; transitory or continuous treated) and then frozen. After FACS-sorting or mock FACS-sorting cells were frozen.
Reference:
Gifford ML, Dean A, Gutierrez RA, Coruzzi GM and Birnbaum KD; PNAS 2009
About the ExperimenterName: | Dr Miriam Gifford |
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Head of Lab Name: | Prof Gloria Coruzzi |
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Lab:
| Center for Genomics and Systems Biology, New York University |
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Address: | 1009 Silver Building 100 Washington Square East New York
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Postcode:
| NY10003 |
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Country:
| USA |
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| Telephone Number:
| 0012129954918 |
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Fax Number:
| 0012129954986 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| organism_part_comparison_design; compound_treatment; time_series |
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Number of Slides: | 91 |
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| Experimental Parameters:
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parameter | organism_part |
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parameter | compound_based_treatment |
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parameter | Nutrients |
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parameter | timepoint |
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Quality Control Measures Taken:
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References:
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Reference | Gifford ML, Dean A, Gutierrez RA, Coruzzi GM and Birnbaum KD; PNAS 2009 |
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Gifford: Cell-specific nitrogen responses in the Arabidopsis root_genome
Slide: Gifford_1-70_Whole-Roots-Frozen-KCl_Rep4_ATH1 | | |
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Tissue:
| Whole roots |
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in vivo Treatment:
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Treatment | KCL (5mM) for 2 hours |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Whole roots 2hr KCl control treated then frozen, biological rep4 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-69_Whole-Roots-Frozen-KCl_Rep3_ATH1 | | |
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Tissue:
| Whole roots |
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in vivo Treatment:
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Treatment | KCL (5mM) for 2 hours |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Whole roots 2hr KCl control treated then frozen, biological rep3 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-68_Whole-Roots-Frozen-KCl_Rep2_ATH1 | | |
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Tissue:
| Whole roots |
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in vivo Treatment:
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Treatment | KCL (5mM) for 2 hours |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Whole roots 2hr KCl control treated then frozen, biological rep2 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-67_Whole-Roots-Frozen-KCl_Rep1_ATH1 | | |
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Tissue:
| Whole roots |
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in vivo Treatment:
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Treatment | KCL (5mM) for 2 hours |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Whole roots 2hr KCl control treated then frozen, biological rep1 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-66_Whole-Roots-Frozen-3_5hr-KNO3_Rep3_ATH1 | | |
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Tissue:
| Whole roots |
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in vivo Treatment:
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Treatment | KNO3 (5mM) for 3.5 hours |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Whole roots 3.5hr KNO3 treated then frozen, biological rep3 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-65_Whole-Roots-Frozen-3_5hr-KNO3_Rep2_ATH1 | | |
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Tissue:
| Whole roots |
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in vivo Treatment:
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Treatment | KNO3 (5mM) for 3.5 hours |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Whole roots 3.5hr KNO3 treated then frozen, biological rep2 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-64_Whole-Roots-Frozen-3_5hr-KNO3_Rep1_ATH1 | | |
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Tissue:
| Whole roots |
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in vivo Treatment:
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Treatment | KNO3 (5mM) for 3.5 hours |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Whole roots 3.5hr KNO3 treated then frozen, biological rep1 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-63_Whole-Roots-Frozen-3_5hr-KCl_Rep3_ATH1 | | |
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Tissue:
| Whole roots |
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in vivo Treatment:
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Treatment | KCL (5mM) for 3.5 hours |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Whole roots 3.5hr KCl control treated then frozen, biological rep3 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-62_Whole-Roots-Frozen-3_5hr-KCl_Rep2_ATH1 | | |
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Tissue:
| Whole roots |
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in vivo Treatment:
| |
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Treatment | KCL (5mM) for 3.5 hours |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Whole roots 3.5hr KCl control treated then frozen, biological rep2 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-61_Whole-Roots-Frozen-3_5hr-KCl_Rep1_ATH1 | | |
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Tissue:
| Whole roots |
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in vivo Treatment:
| |
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Treatment | KCL (5mM) for 3.5 hours |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Whole roots 3.5hr KCl control treated then frozen, biological rep1 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-60_Protoplasts-KNO3_Rep3_ATH1 | | |
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Tissue:
| Root protoplasts |
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in vivo Treatment:
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Treatment | KNO3 (5mM) for 2 hours then KN03 maintained during protoplasting (3.5 hr total) |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Protoplasted root cells 2hr continuous KNO3 treated, biological rep3 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-59_Protoplasts-KNO3_Rep2_ATH1 | | |
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Tissue:
| Root protoplasts |
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in vivo Treatment:
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Treatment | KNO3 (5mM) for 2 hours then KN03 maintained during protoplasting (3.5 hr total) |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Protoplasted root cells 2hr continuous KNO3 treated, biological rep2 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-58_Protoplasts-KNO3_Rep1_ATH1 | | |
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Tissue:
| Root protoplasts |
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in vivo Treatment:
| |
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Treatment | KNO3 (5mM) for 2 hours then KN03 maintained during protoplasting (3.5 hr total) |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Protoplasted root cells 2hr continuous KNO3 treated, biological rep1 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-57_Protoplasts-KCl_Rep3_ATH1 | | |
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Tissue:
| Root protoplasts |
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in vivo Treatment:
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Treatment | KCL (5mM) for 2 hours |
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Additional Organism Information:
| |
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Protoplasted root cells 2hr KCl control treated, biological rep3 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-56_Protoplasts-KCl_Rep2_ATH1 | | |
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Tissue:
| Root protoplasts |
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in vivo Treatment:
| |
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Treatment | KCL (5mM) for 2 hours |
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Additional Organism Information:
| |
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Protoplasted root cells 2hr KCl control treated, biological rep2 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-55_Protoplasts-KCl_Rep1_ATH1 | | |
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Tissue:
| Root protoplasts |
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in vivo Treatment:
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Treatment | KCL (5mM) for 2 hours |
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Additional Organism Information:
| |
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Protoplasted root cells 2hr KCl control treated, biological rep1 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-54_Stele-Transitory-KNO3_Rep3_ATH1 | | |
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Tissue:
| FACS-sorted stele cells |
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in vivo Treatment:
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Treatment | KNO3 (5mM) for 2 hours |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Stele root cells 2hr transitory KNO3 treated, biological rep3 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-53_Stele-Transitory-KNO3_Rep2_ATH1 | | |
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Tissue:
| FACS-sorted stele cells |
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in vivo Treatment:
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Treatment | KNO3 (5mM) for 2 hours |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Stele root cells 2hr transitory KNO3 treated, biological rep2 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-52_Stele-Transitory-KNO3_Rep1_ATH1 | | |
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Tissue:
| FACS-sorted stele cells |
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in vivo Treatment:
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Treatment | KNO3 (5mM) for 2 hours |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Stele root cells 2hr transitory KNO3 treated, biological rep1 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-51_Stele-KCl_Rep3_ATH1 | | |
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Tissue:
| FACS-sorted stele cells |
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in vivo Treatment:
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Treatment | KCL (5mM) for 2 hours |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Stele root cells 2hr KCl control treated, biological rep3 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-50_Stele-KCl_Rep2_ATH1 | | |
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Tissue:
| FACS-sorted stele cells |
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in vivo Treatment:
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Treatment | KCL (5mM) for 2 hours |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Stele root cells 2hr KCl control treated, biological rep2 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-49_Stele-KCl_Rep1_ATH1 | | |
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Tissue:
| FACS-sorted stele cells |
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in vivo Treatment:
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Treatment | KCL (5mM) for 2 hours |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Stele root cells 2hr KCl control treated, biological rep1 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-48_Stele-Continuous-KNO3_Rep3_ATH1 | | |
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Tissue:
| FACS-sorted stele cells |
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in vivo Treatment:
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Treatment | KNO3 (5mM) for 2 hours then KN03 maintained during protoplasting (3.5 hr total) |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Stele root cells 2hr continuous KNO3 treated, biological rep3 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-47_Stele-Continuous-KNO3_Rep2_ATH1 | | |
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Tissue:
| FACS-sorted stele cells |
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in vivo Treatment:
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Treatment | KNO3 (5mM) for 2 hours then KN03 maintained during protoplasting (3.5 hr total) |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Stele root cells 2hr continuous KNO3 treated, biological rep2 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-46_Stele-Continuous-KNO3_Rep1_ATH1 | | |
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Tissue:
| FACS-sorted stele cells |
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in vivo Treatment:
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Treatment | KNO3 (5mM) for 2 hours then KN03 maintained during protoplasting (3.5 hr total) |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Stele root cells 2hr continuous KNO3 treated, biological rep1 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-45_Peri-MSX-Continuous-KNO3-Gln_Rep3_ATH1 | | |
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Tissue:
| FACS-sorted pericycle cells |
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in vivo Treatment:
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Treatment | KNO3 (5mM) and MSX (1mM) and Gln (5mM) for 2 hours then all maintained during protoplasting (3.5 hr total) |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Pericycle root cells 2hr continuous KNO3 and MSX and Gln treated, biological rep3 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-44_Peri-MSX-Continuous-KNO3-Gln_Rep2_ATH1 | | |
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Tissue:
| FACS-sorted pericycle cells |
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in vivo Treatment:
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Treatment | KNO3 (5mM) and MSX (1mM) and Gln (5mM) for 2 hours then all maintained during protoplasting (3.5 hr total) |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Pericycle root cells 2hr continuous KNO3 and MSX and Gln treated, biological rep2 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-43_Peri-MSX-Continuous-KNO3-Gln_Rep1_ATH1 | | |
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Tissue:
| FACS-sorted pericycle cells |
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in vivo Treatment:
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Treatment | KNO3 (5mM) and MSX (1mM) and Gln (5mM) for 2 hours then all maintained during protoplasting (3.5 hr total) |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Pericycle root cells 2hr continuous KNO3 and MSX and Gln treated, biological rep1 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-42_Peri-MSX-Continuous-KNO3_Rep3_ATH1 | | |
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Tissue:
| FACS-sorted pericycle cells |
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in vivo Treatment:
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Treatment | KNO3 (5mM) and MSX (1mM) for 2 hours then both maintained during protoplasting (3.5 hr total) |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Pericycle root cells 2hr continuous KNO3 and MSX control treated, biological rep3 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-41_Peri-MSX-Continuous-KNO3_Rep2_ATH1 | | |
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Tissue:
| FACS-sorted pericycle cells |
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in vivo Treatment:
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Treatment | KNO3(5mM) and MSX (1mM) for 2 hours then both maintained during protoplasting (3.5 hr total) |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Pericycle root cells 2hr continuous KNO3 and MSX control treated, biological rep2 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-40_Peri-MSX-Continuous-KNO3_Rep1_ATH1 | | |
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Tissue:
| FACS-sorted pericycle cells |
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in vivo Treatment:
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Treatment | KNO3 (5mM) and MSX (1mM) for 2 hours then both maintained during protoplasting (3.5 hr total) |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Pericycle root cells 2hr continuous KNO3 and MSX control treated, biological rep1 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-39_Peri-KCl-MSX-for-Continuous-KNO3_Rep3_ATH1 | | |
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Tissue:
| FACS-sorted pericycle cells |
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in vivo Treatment:
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Treatment | KCl (5mM) and MSX (1mM) for 2 hours then both maintained during protoplasting (3.5 hr total) |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Pericycle root cells 2hr continuous KCl and MSX treated, biological rep3 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-38_Peri-KCl-MSX-for-Continuous-KNO3_Rep2_ATH1 | | |
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Tissue:
| FACS-sorted pericycle cells |
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in vivo Treatment:
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Treatment | KCl (5mM) and MSX (1mM) for 2 hours then both maintained during protoplasting (3.5 hr total) |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Pericycle root cells 2hr continuous KCl and MSX treated, biological rep2 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-37_Peri-KCl-MSX-for-Continuous-KNO3_Rep1_ATH1 | | |
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Tissue:
| FACS-sorted pericycle cells |
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in vivo Treatment:
| |
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Treatment | KCl (5mM) and MSX (1mM) for 2 hours then both maintained during protoplasting (3.5 hr total) |
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Additional Organism Information:
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Pericycle root cells 2hr continuous KCl and MSX treated, biological rep1 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-36_Peri-Transitory-KNO3_Rep3_ATH1 | | |
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Tissue:
| FACS-sorted pericycle cells |
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in vivo Treatment:
| |
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Treatment | KNO3 (5mM) for 2 hours |
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Additional Organism Information:
| |
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
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Sample description | Pericycle root cells 2hr transitory KNO3 treated, biological rep3 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-35_Peri-Transitory-KNO3_Rep2_ATH1 | | |
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Tissue:
| FACS-sorted pericycle cells |
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in vivo Treatment:
| |
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Treatment | KNO3 (5mM) for 2 hours |
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Additional Organism Information:
| |
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
| |
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Sample description | Pericycle root cells 2hr transitory KNO3 treated, biological rep2 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-34_Peri-Transitory-KNO3_Rep1_ATH1 | | |
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Tissue:
| FACS-sorted pericycle cells |
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in vivo Treatment:
| |
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Treatment | KNO3 (5mM) for 2 hours |
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Additional Organism Information:
| |
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
| |
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Sample description | Pericycle root cells 2hr transitory KNO3 treated, biological rep1 |
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Protocols for BioSource 1 |
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Slide: Gifford_1-33_Peri-KCl_Rep3_ATH1 | | |
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Tissue:
| FACS-sorted pericycle cells |
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in vivo Treatment:
| |
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Treatment | KCL (5mM) for 2 hours |
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Additional Organism Information:
| |
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| Long day conditions; seedlings treated at dawn on day 12 |
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Other Information:
| |
---|
Sample description | Pericycle root cells 2hr KCl control treated, biological rep3 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-32_Peri-KCl_Rep2_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted pericycle cells |
---|
in vivo Treatment:
| |
---|
Treatment | KCL (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Pericycle root cells 2hr KCl control treated, biological rep2 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-31_Peri-KCl_Rep1_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted pericycle cells |
---|
in vivo Treatment:
| |
---|
Treatment | KCL (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Pericycle root cells 2hr KCl control treated, biological rep1 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-30_Peri-Continuous-KNO3_Rep3_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted pericycle cells |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours then KN03 maintained during protoplasting (3.5 hr total) |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Pericycle root cells 2hr continuous KNO3 treated, biological rep3 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-29_Peri-Continuous-KNO3_Rep2_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted pericycle cells |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours then KN03 maintained during protoplasting (3.5 hr total) |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Pericycle root cells 2hr continuous KNO3 treated, biological rep2 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-28_Peri-Continuous-KNO3_Rep1_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted pericycle cells |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours then KN03 maintained during protoplasting (3.5 hr total) |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Pericycle root cells 2hr continuous KNO3 treated, biological rep1 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-27_LRC-Transitory-KNO3_Rep3_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted lateral root cap cells |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Lateral Root Cap root cells 2hr transitory KNO3 treated, biological rep3 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-26_LRC-Transitory-KNO3_Rep2_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted lateral root cap cells |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Lateral Root Cap root cells 2hr transitory KNO3 treated, biological rep2 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-25_LRC-Transitory-KNO3_Rep1_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted lateral root cap cells |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Lateral Root Cap root cells 2hr transitory KNO3 treated, biological rep1 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-24_LRC-KCl_Rep3_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted lateral root cap cells |
---|
in vivo Treatment:
| |
---|
Treatment | KCL (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Lateral Root Cap root cells 2hr KCl control treated, biological rep3 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-23_LRC-KCl_Rep2_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted lateral root cap cells |
---|
in vivo Treatment:
| |
---|
Treatment | KCL (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Lateral Root Cap root cells 2hr KCl control treated, biological rep2 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-22_LRC-KCl_Rep1_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted lateral root cap cells |
---|
in vivo Treatment:
| |
---|
Treatment | KCL (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Lateral Root Cap root cells 2hr KCl control treated, biological rep1 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-21_LRC-Continuous-KNO3_Rep3_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted lateral root cap cells |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours then KN03 maintained during protoplasting (3.5 hr total) |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Lateral Root Cap root cells 2hr continuous KNO3 treated, biological rep3 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-20_LRC-Continuous-KNO3_Rep2_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted lateral root cap cells |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours then KN03 maintained during protoplasting (3.5 hr total) |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Lateral Root Cap root cells 2hr continuous KNO3 treated, biological rep2 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-19_LRC-Continuous-KNO3_Rep1_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted lateral root cap cells |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours then KN03 maintained during protoplasting (3.5 hr total) |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Lateral Root Cap root cells 2hr continuous KNO3 treated, biological rep1 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-18_EpiC-Transitory-KNO3_Rep3_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted epidermis and cortex cells |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Epidermis and Cortex root cells 2hr transitory KNO3 treated, biological rep3 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-17_EpiC-Transitory-KNO3_Rep2_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted epidermis and cortex cells |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Epidermis and Cortex root cells 2hr transitory KNO3 treated, biological rep2 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-16_EpiC-Transitory-KNO3_Rep1_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted epidermis and cortex cells |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Epidermis and Cortex root cells 2hr transitory KNO3 treated, biological rep1 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-15_EpiC-KCl_Rep3_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted epidermis and cortex cells |
---|
in vivo Treatment:
| |
---|
Treatment | KCL (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Epidermis and Cortex root cells 2hr KCl control treated, biological rep3 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-14_EpiC-KCl_Rep2_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted epidermis and cortex cells |
---|
in vivo Treatment:
| |
---|
Treatment | KCL (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Epidermis and Cortex root cells 2hr KCl control treated, biological rep2 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-13_EpiC-KCl_Rep1_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted epidermis and cortex cells |
---|
in vivo Treatment:
| |
---|
Treatment | KCL (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Epidermis and Cortex root cells 2hr KCl control treated, biological rep1 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-12_EpiC-Continuous-KNO3_Rep3_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted epidermis and cortex cells |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours then KN03 maintained during protoplasting (3.5 hr total) |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Epidermis and Cortex root cells 2hr continuous KNO3 treated, biological rep3 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-11_EpiC-Continuous-KNO3_Rep2_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted epidermis and cortex cells |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours then KN03 maintained during protoplasting (3.5 hr total) |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Epidermis and Cortex root cells 2hr continuous KNO3 treated, biological rep2 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-10_EpiC-Continuous-KNO3_Rep1_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted epidermis and cortex cells |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours then KN03 maintained during protoplasting (3.5 hr total) |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Epidermis and Cortex root cells 2hr continuous KNO3 treated, biological rep1 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-9_EndoP-Transitory-KNO3_Rep3_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted endodermis and pericycle cells |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Endodermis and Pericycle root cells 2hr transitory KNO3 treated, biological rep3 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-8_EndoP-Transitory-KNO3_Rep2_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted endodermis and pericycle cells |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Endodermis and Pericycle root cells 2hr transitory KNO3 treated, biological rep2 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-7_EndoP-Transitory-KNO3_Rep1_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted endodermis and pericycle cells |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Endodermis and Pericycle root cells 2hr transitory KNO3 treated, biological rep1 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-6_EndoP-KCl_Rep3_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted endodermis and pericycle cells |
---|
in vivo Treatment:
| |
---|
Treatment | KCL (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Endodermis and Pericycle root cells 2hr KCl control treated, biological rep3 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-5_EndoP-KCl_Rep2_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted endodermis and pericycle cells |
---|
in vivo Treatment:
| |
---|
Treatment | KCL (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Endodermis and Pericycle root cells 2hr KCl control treated, biological rep2 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-4_EndoP-KCl_Rep1_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted endodermis and pericycle cells |
---|
in vivo Treatment:
| |
---|
Treatment | KCL (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Endodermis and Pericycle root cells 2hr KCl control treated, biological rep1 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-3_EndoP-Continuous-KNO3_Rep3_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted endodermis and pericycle cells |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours then KN03 maintained during protoplasting (3.5 hr total) |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Endodermis and Pericycle root cells 2hr continuous KNO3 treated, biological rep3 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-2_EndoP-Continuous-KNO3_Rep2_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted endodermis and pericycle cells |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours then KN03 maintained during protoplasting (3.5 hr total) |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Endodermis and Pericycle root cells 2hr continuous KNO3 treated, biological rep2 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-1_EndoP-Continuous-KNO3_Rep1_ATH1 | | |
|
|
|
Tissue:
| FACS-sorted endodermis and pericycle cells |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours then KN03 maintained during protoplasting (3.5 hr total) |
---|
Additional Organism Information:
| |
---|
| ">Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Endodermis and Pericycle root cells 2hr continuous KNO3 treated, biological rep1 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-76_Whole-Roots-Frozen-KNO3_Rep4_ATH1 | | |
|
|
|
Tissue:
| Whole roots |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Whole roots 2hr KNO3 treated then frozen, biological rep4 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-75_Whole-Roots-Frozen-KNO3_Rep3_ATH1 | | |
|
|
|
Tissue:
| Whole roots |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Whole roots 2hr KNO3 treated then frozen, biological rep3 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-74_Whole-Roots-Frozen-KNO3_Rep2_ATH1 | | |
|
|
|
Tissue:
| Whole roots |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Whole roots 2hr KNO3 treated then frozen, biological rep2 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-73_Whole-Roots-Frozen-KNO3_Rep1_ATH1 | | |
|
|
|
Tissue:
| Whole roots |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Whole roots 2hr KNO3 treated then frozen, biological rep1 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-72_Whole-Roots-Frozen-KCl_Rep6_ATH1 | | |
|
|
|
Tissue:
| Whole roots |
---|
in vivo Treatment:
| |
---|
Treatment | KCL (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Whole roots 2hr KCl control treated then frozen, biological rep6 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-71_Whole-Roots-Frozen-KCl_Rep5_ATH1 | | |
|
|
|
Tissue:
| Whole roots |
---|
in vivo Treatment:
| |
---|
Treatment | KCL (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Whole roots 2hr KCl control treated then frozen, biological rep5 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-77_Whole-Roots-Frozen-KNO3_Rep5_ATH1 | | |
|
|
|
Tissue:
| Whole roots |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Whole roots 2hr KNO3 treated then frozen, biological rep5 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-78_Whole-Roots-Frozen-KNO3_Rep6_ATH1 | | |
|
|
|
Tissue:
| Whole roots |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Whole roots 2hr KNO3 treated then frozen, biological rep6 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-79_Whole-Roots-Frozen-KNO3_Rep7_ATH1 | | |
|
|
|
Tissue:
| Whole roots |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Whole roots 2hr KNO3 treated then frozen, biological rep7 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-80_Whole-Roots-Frozen-KNO3_Rep8_ATH1 | | |
|
|
|
Tissue:
| Whole roots |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Whole roots 2hr KNO3 treated then frozen, biological rep8 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-81_Whole-Roots-Incubated-Continuous-KNO3_Rep1_ATH1 | | |
|
|
|
Tissue:
| Whole roots |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours then KN03 maintained during incubation (3.5 hr total) |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Whole roots 2hr KNO3 treated then incubated in protoplast-generating solution minus enzymes with KNO3, biological rep1 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-82_Whole-Roots-Incubated-Continuous-KNO3_Rep2_ATH1 | | |
|
|
|
Tissue:
| Whole roots |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours then KN03 maintained during incubation (3.5 hr total) |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Whole roots 2hr KNO3 treated then incubated in protoplast-generating solution minus enzymes with KNO3, biological rep2 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-83_Whole-Roots-Incubated-Continuous-KNO3_Rep3_ATH1 | | |
|
|
|
Tissue:
| Whole roots |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours then KN03 maintained during incubation (3.5 hr total) |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Whole roots 2hr KNO3 treated then incubated in protoplast-generating solution minus enzymes with KNO3, biological rep3 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-84_Whole-Roots-Incubated-KCl_Rep1_ATH1 | | |
|
|
|
Tissue:
| Whole roots |
---|
in vivo Treatment:
| |
---|
Treatment | KCL (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Whole roots 2hr KCl treated then incubated in protoplast-generating solution minus enzymes, biological rep1 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-85_Whole-Roots-Incubated-KCl_Rep2_ATH1 | | |
|
|
|
Tissue:
| Whole roots |
---|
in vivo Treatment:
| |
---|
Treatment | KCL (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Whole roots 2hr KCl treated then incubated in protoplast-generating solution minus enzymes, biological rep2 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-86_Whole-Roots-Incubated-KCl_Rep3_ATH1 | | |
|
|
|
Tissue:
| Whole roots |
---|
in vivo Treatment:
| |
---|
Treatment | KCL (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Whole roots 2hr KCl treated then incubated in protoplast-generating solution minus enzymes, biological rep3 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-87_Whole-Roots-Incubated-KCl_Rep4_ATH1 | | |
|
|
|
Tissue:
| Whole roots |
---|
in vivo Treatment:
| |
---|
Treatment | KCL (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Whole roots 2hr KCl treated then incubated in protoplast-generating solution minus enzymes, biological rep4 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-88_Whole-Roots-Incubated-Transitory-KNO3_Rep1_ATH1 | | |
|
|
|
Tissue:
| Whole roots |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Whole roots 2hr KNO3 treated then incubated in protoplast-generating solution minus enzymes, biological rep1 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-89_Whole-Roots-Incubated-Transitory-KNO3_Rep2_ATH1 | | |
|
|
|
Tissue:
| Whole roots |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Whole roots 2hr KNO3 treated then incubated in protoplast-generating solution minus enzymes, biological rep2 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-90_Whole-Roots-Incubated-Transitory-KNO3_Rep3_ATH1 | | |
|
|
|
Tissue:
| Whole roots |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Whole roots 2hr KNO3 treated then incubated in protoplast-generating solution minus enzymes, biological rep3 |
---|
|
Protocols for BioSource 1 |
|
|
|
Slide: Gifford_1-91_Whole-Roots-Incubated-Transitory-KNO3_Rep4_ATH1 | | |
|
|
|
Tissue:
| Whole roots |
---|
in vivo Treatment:
| |
---|
Treatment | KNO3 (5mM) for 2 hours |
---|
Additional Organism Information:
| |
---|
| Long day conditions; seedlings treated at dawn on day 12 |
---|
Other Information:
| |
---|
Sample description | Whole roots 2hr KNO3 treated then incubated in protoplast-generating solution minus enzymes, biological rep4 |
---|
|
Protocols for BioSource 1 |
|
|
|
Problems? Comments? Suggestions? Contact the Affymetrix Team