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Experiment: ERFVII and hypoxia
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-588
The aim of this analysis is to compare plants with increased expression of At1g53910 and decreased expression of At1g53910 and At3g14230 in their response to hypoxia (1% O2). HA-tagged overexpressors of At1g53910 and knock-out lines obtained by overexpression of an artificial microRNA against At1g53910 and At3g14230 were used. Plants were treated for 90 mins under hypoxic or normoxic conditions and then the above-ground tissues were collected and rapidly frozen in liquid nitrogen. RNA was extracted using the RNeasy (Qiagen) extraction kit.Control wt are also used to test differentially regulated genes in plants overexpressing an HA tagged version of At5g21960.
About the ExperimenterName: | Mr Francesco Licausi |
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Head of Lab Name: | Dr Joost van Dongen |
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Lab:
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Institute:
| Department 1: Molecular Physiology |
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Address: | Max-Planck-Institut fur Molekulare Pflanzenphysiologie Wissenschaftspark Golm, Am Muhlenberg 1, Potsdam, Golm, Brandenburg
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Postcode:
| 14476 |
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Country:
| GERMANY |
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| Telephone Number:
| +49 0331 567 8353 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| genetic_modification_design, stimulus_or_stress_design |
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Number of Slides: | 20 |
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| Experimental Parameters:
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parameter | change_oxygen_concentration |
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parameter | genetic_modification |
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Quality Control Measures Taken:
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Licausi: ERFVII and hypoxia
Slide: Licausi_588-1_WT_Control_Rep1_ATH1 | | |
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in vivo Treatment:
| Plants treated for 90 min in the dark at normoxic conditions (21% O2). |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Licausi_588-2_WT_Control_Rep2_ATH1 | | |
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in vivo Treatment:
| Plants treated for 90 min in the dark at normoxic conditions (21% O2). |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Licausi_588-3_WT_Control_Rep3_ATH1 | | |
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in vivo Treatment:
| Plants treated for 90 min in the dark at normoxic conditions (21% O2). |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Licausi_588-4_WT_Hypo_Rep1_ATH1 | | |
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in vivo Treatment:
| Plants treated for 90 min in the dark at hypoxia conditions (1% O2). |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Licausi_588-5_WT_Hypo_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Plants treated for 90 min in the dark at hypoxia conditions (1% O2). |
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Additional Organism Information:
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Sample Description | healthy green plants with 3cm diameter rosette. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Licausi_588-6_WT_Hypo_Rep3_ATH1 | | |
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Tissue:
| whole rosette |
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in vivo Treatment:
| Plants treated for 90 min in the dark at hypoxia conditions (1% O2). |
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Additional Organism Information:
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Sample Description | healthy green plants with 3cm diameter rosette. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Licausi_588-7_HA-RAP2.12_Control_Rep1_ATH1 | | |
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Tissue:
| whole rosette |
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in vivo Treatment:
| Plants treated for 90 min in the dark at normoxic conditions (1% O2). |
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Additional Organism Information:
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Sample Description | healthy green plants with 3cm diameter rosette. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Licausi_588-8_HA-RAP2.12_Control_Rep2_ATH1 | | |
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Tissue:
| whole rosette |
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in vivo Treatment:
| Plants treated for 90 min in the dark at normoxic conditions (1% O2). |
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Additional Organism Information:
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Sample Description | healthy green plants with 3cm diameter rosette. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Licausi_588-9_HA-RAP2.12_Control_Rep3_ATH1 | | |
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Tissue:
| whole rosette |
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in vivo Treatment:
| Plants treated for 90 min in the dark at normoxic conditions (1% O2). |
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Additional Organism Information:
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Sample Description | healthy green plants with 3cm diameter rosette. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Licausi_588-10_HA-RAP2.12_Hypo_Rep1_ATH1 | | |
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Tissue:
| whole rosette |
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in vivo Treatment:
| Plants treated for 90 min in the dark at hypoxic conditions (21% O2). |
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Additional Organism Information:
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Sample Description | healthy green plants with 3cm diameter rosette. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Licausi_588-11_HA-RAP2.12_Hypo_Rep2_ATH1 | | |
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Tissue:
| whole rosette |
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in vivo Treatment:
| Plants treated for 90 min in the dark at hypoxic conditions (1% O2). |
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Additional Organism Information:
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Sample Description | healthy green plants with 3cm diameter rosette. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Licausi_588-12_HA-RAP2.12_Hypo_Rep3_ATH1 | | |
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Tissue:
| whole rosette |
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in vivo Treatment:
| Plants treated for 90 min in the dark at hypoxic conditions (1% O2). |
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Additional Organism Information:
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Sample Description | healthy green plants with 3cm diameter rosette. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Licausi_588-13_ami-RAP_Control_Rep1_ATH1 | | |
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Tissue:
| whole rosette |
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in vivo Treatment:
| Plants treated for 90 min in the dark at normoxic conditions (21% O2). |
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Additional Organism Information:
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Sample Description | healthy green plants with 3cm diameter rosette. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Licausi_588-14_ami-RAP_Control_Rep2_ATH1 | | |
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Tissue:
| whole rosette |
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in vivo Treatment:
| Plants treated for 90 min in the dark at normoxic conditions (21% O2). |
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Additional Organism Information:
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Sample Description | healthy green plants with 3cm diameter rosette. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Licausi_588-15_ami-RAP_Control_Rep3_ATH1 | | |
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Tissue:
| whole rosette |
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in vivo Treatment:
| Plants treated for 90 min in the dark at normoxic conditions (21% O2). |
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Additional Organism Information:
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Sample Description | healthy green plants with 3cm diameter rosette. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Licausi_588-16_ami-RAP_Hypo_Rep1_ATH1 | | |
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Tissue:
| whole rosette |
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in vivo Treatment:
| Plants treated for 90 min in the dark at normoxic conditions (21% O2). |
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Additional Organism Information:
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Sample Description | healthy green plants with 3cm diameter rosette. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Licausi_588-17_ami-RAP_Hypo_Rep2_ATH1 | | |
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Tissue:
| whole rosette |
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in vivo Treatment:
| Plants treated for 90 min in the dark at normoxic conditions (21% O2). |
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Additional Organism Information:
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Sample Description | healthy green plants with 3cm diameter rosette. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Licausi_588-18_ami-RAP_Hypo_Rep3_ATH1 | | |
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Tissue:
| whole rosette |
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in vivo Treatment:
| Plants treated for 90 min in the dark at hypoxic conditions (1% O2). |
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Additional Organism Information:
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Sample Description | healthy green plants with 3cm diameter rosette. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Licausi_588-19_35S::HA::ERF16 _Rep1_ATH1 | | |
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Tissue:
| whole rosette |
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Additional Organism Information:
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Sample Description | healthy green plants with 3cm diameter rosette. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Licausi_588-20_35S::HA::ERF16 _Rep2_ATH1 | | |
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Tissue:
| whole rosette |
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Additional Organism Information:
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Sample Description | healthy green plants with 3cm diameter rosette. |
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Other Information:
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Protocols for BioSource 1 |
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Problems? Comments? Suggestions? Contact the Affymetrix Team