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Experiment: Analysis MYB26 targets by using Dex inducible line
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-635
MALE STERILITY 35 (MS35/ MYB26) is a R2R3-type MYB transcription factor (Steiner-Large et al., 2003). MYB26 is suggested to be a key regulator in the process of lignified secondary thickening in the anther endothecium which provides mechanical force for anther dehiscence (Dawson et al., 1999). This microarray experiment aims to identify genes controlled by MYB26 and thus establish the precise role MYB26 plays during anther development. Comparisons of gene expression will be carried out between functional complemented ms35 mutant line-MYB26promoter::MYB26gDNA-GRYFP and ms35 mutant at the time points 0h, 2h, 4h and 6h after Dex treatment to identify genes with altered expression due to the functional MYB26 released by the Dex induction. The Dex treated ms35 mutant was chosen to ensure that the changes of gene expression are not due to the Dex effect. Plants were grown in growth room at 22h of daylight (167 umol.m2.sec-1) at 24 C and 2h of night at 24 C. Flowering plants with 3-5 sterile siliques were sprayed with 25uM Dex; closed buds were collected from both lines at the time points 0h, 2h, 4h and 6h. RNA was extracted using RNeasy Mini Kit (Qiagen, USA) and digested with DNase to eliminate genomic DNA contamination.
About the ExperimenterName: | Dr Caiyun Yang |
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Head of Lab Name: | Zoe Wilson |
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Lab:
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Address: | Plant and Crop Sciences Division, Plant Sciences Building, School of Biosciences, University of Nottingham Sutton Bonington Campus, College Road, Sutton Bonington, Nottinghamshire, LE12 5RD, UNITED KINGDOM
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Postcode:
| LE125RD |
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Country:
| United Kingdom |
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| Telephone Number:
| 01159516383 |
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Fax Number:
| 01159516334 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| timepoint |
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Number of Slides: | 16 |
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| Experimental Parameters:
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parameter | timepoint |
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Quality Control Measures Taken:
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References:
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Reference | Steiner-Large et al., 2003 |
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Reference | Dawson et al., 1999 |
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Yang: Analysis MYB26 targets by using Dex inducible line
Slide: Yang_635-10_Dex _2h_buds_MYB26-ms35_Rep2_ATH1 | | |
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Tissue:
| buds |
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in vivo Treatment:
| When plants flowering with 3-5 silique (5 weeks), 25uM Dex +0.02% Silwet was spayed onto infloroscences and covered with lid. All unopened flower buds from primary infloroscences of 5 plats were collected. |
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Additional Organism Information:
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Sample Description | All unopened flower buds from primary infloroscences of 5 plants were collected at 2 hours after Dex treatment. |
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Other Information:
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Timecourse start procedure | when Dex was applied to plants |
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Protocols for BioSource 1 |
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Slide: Yang_635-9_Dex _0h_buds_MYB26-ms35_Rep2_ATH1 | | |
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Tissue:
| buds |
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Additional Organism Information:
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Sample Description | All unopened flower buds from primary infloroscences of 5 plants were collected before Dex treatment. |
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Other Information:
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Timecourse start procedure | Just before Dex was applied to plants |
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Protocols for BioSource 1 |
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Slide: Yang_635-7_Dex_4h_buds_ms35 mutant_Rep1_ATH1 | | |
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Tissue:
| buds |
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in vivo Treatment:
| When plants flowering with 3-5 silique (5 weeks), 25uM Dex +0.02% Silwet was spayed onto infloroscences and covered with lid. All unopened flower buds from primary infloroscences of 5 plats were collected. |
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Additional Organism Information:
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Sample Description | All unopened flower buds from primary infloroscences of 5 plants were collected 4 hours after Dex treatment. |
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Other Information:
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Timecourse start procedure | When Dex was applied to plants |
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Protocols for BioSource 1 |
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Slide: Yang_635-6_Dex_2h_buds_ms35 mutant_Rep1_ATH1 | | |
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Tissue:
| buds |
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in vivo Treatment:
| When plants flowering with 3-5 silique (5 weeks), 25uM Dex +0.02% Silwet was spayed onto infloroscences and covered with lid. All unopened flower buds from primary infloroscences of 5 plats were collected. |
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Additional Organism Information:
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Sample Description | All unopened flower buds from primary infloroscences of 5 plants were collected before Dex treatment. |
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Other Information:
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Timecourse start procedure | When Dex was applied to plants |
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Protocols for BioSource 1 |
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Slide: Yang_635-5_Dex_0h_buds_ms35 mutant_Rep1_ATH1 | | |
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Tissue:
| buds |
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Additional Organism Information:
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Sample Description | All unopened flower buds from primary infloroscences of 5 plants were collected before Dex treatment. |
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Other Information:
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Timecourse start procedure | Just before Dex was applied to plants |
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Protocols for BioSource 1 |
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Slide: Yang_635-4_Dex _6h_buds_MYB26-ms35_Rep1_ATH1 | | |
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Tissue:
| buds |
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in vivo Treatment:
| When plants flowering with 3-5 silique (5 weeks), 25uM Dex +0.02% Silwet was spayed onto infloroscences and covered with lid. All unopened flower buds from primary infloroscences of 5 plats were collected. |
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Additional Organism Information:
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Sample Description | All unopened flower buds from primary infloroscences of 5 plants were collected at 6 hours after Dex treatment. |
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Other Information:
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Timecourse start procedure | when Dex was applied to plants |
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Protocols for BioSource 1 |
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Slide: Yang_635-3_Dex _4h_buds_MYB26-ms35_Rep1_ATH1 | | |
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Tissue:
| buds |
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in vivo Treatment:
| When plants flowering with 3-5 silique (5 weeks), 25uM Dex +0.02% Silwet was spayed onto infloroscences and covered with lid. All unopened flower buds from primary infloroscences of 5 plats were collected. |
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Additional Organism Information:
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Sample Description | All unopened flower buds from primary infloroscences of 5 plants were collected at 4 hours after Dex treatment. |
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Other Information:
| |
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Timecourse start procedure | when Dex was applied to plants |
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|
Protocols for BioSource 1 |
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Slide: Yang_635-1_Dex _0h_buds_MYB26-ms35_Rep1_ATH1 | | |
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Tissue:
| buds |
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Additional Organism Information:
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Sample Description | When plants flowering with 3-5 silique (5 weeks), all unopened flower buds were collected |
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Other Information:
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Timecourse start procedure | Just before the Dex treatment |
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Protocols for BioSource 1 |
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Slide: Yang_635-2_Dex _2h_buds_MYB26-ms35_Rep1_ATH1 | | |
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Tissue:
| buds |
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in vivo Treatment:
| When plants flowering with 3-5 silique (5 weeks), 25uM Dex +0.02% Silwet was spayed onto infloroscences and covered with lid. All unopened flower buds from primary infloroscences of 5 plats were collected. |
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Additional Organism Information:
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Sample Description | All unopened flower buds from primary infloroscences of 5 plants were collected at 2 hours after Dex treatment. |
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Other Information:
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Timecourse start procedure | when Dex was applied to plants |
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Protocols for BioSource 1 |
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Slide: Yang_635-8_Dex_6h_buds_ms35 mutant_Rep1_ATH1 | | |
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Tissue:
| buds |
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in vivo Treatment:
| When plants flowering with 3-5 silique (5 weeks), 25uM Dex +0.02% Silwet was spayed onto infloroscences and covered with lid. All unopened flower buds from primary infloroscences of 5 plats were collected. |
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Additional Organism Information:
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Sample Description | All unopened flower buds from primary infloroscences of 5 plants were collected 6 hours after Dex treatment. |
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Other Information:
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Timecourse start procedure | When Dex was applied to plants |
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Protocols for BioSource 1 |
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Slide: Yang_635-11_Dex _4h_buds_MYB26-ms35_Rep2_ATH1 | | |
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Tissue:
| buds |
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in vivo Treatment:
| When plants flowering with 3-5 silique (5 weeks), 25uM Dex +0.02% Silwet was spayed onto infloroscences and covered with lid. All unopened flower buds from primary infloroscences of 5 plats were collected. |
---|
Additional Organism Information:
| |
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Sample Description | All unopened flower buds from primary infloroscences of 5 plants were collected at 4 hours after Dex treatment. |
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Other Information:
| |
---|
Timecourse start procedure | when Dex was applied to plants |
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|
Protocols for BioSource 1 |
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Slide: Yang_635-16_Dex_6h_buds_ms35 mutant_Rep2_ATH1 | | |
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Tissue:
| buds |
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in vivo Treatment:
| When plants flowering with 3-5 silique (5 weeks), 25uM Dex +0.02% Silwet was spayed onto infloroscences and covered with lid. All unopened flower buds from primary infloroscences of 5 plats were collected. |
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Additional Organism Information:
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Sample Description | All unopened flower buds from primary infloroscences of 5 plants were collected 6 hours after Dex treatment. |
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Other Information:
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Timecourse start procedure | When Dex was applied to plants |
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|
Protocols for BioSource 1 |
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Slide: Yang_635-15_Dex_4h_buds_ms35 mutant_Rep2_ATH1 | | |
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Tissue:
| buds |
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in vivo Treatment:
| When plants flowering with 3-5 silique (5 weeks), 25uM Dex +0.02% Silwet was spayed onto infloroscences and covered with lid. All unopened flower buds from primary infloroscences of 5 plats were collected. |
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Additional Organism Information:
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Sample Description | All unopened flower buds from primary infloroscences of 5 plants were collected 4 hours after Dex treatment. |
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Other Information:
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Timecourse start procedure | When Dex was applied to plants |
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|
Protocols for BioSource 1 |
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Slide: Yang_635-14_Dex_2h_buds_ms35 mutant_Rep2_ATH1 | | |
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Tissue:
| buds |
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in vivo Treatment:
| When plants flowering with 3-5 silique (5 weeks), 25uM Dex +0.02% Silwet was spayed onto infloroscences and covered with lid. All unopened flower buds from primary infloroscences of 5 plats were collected. |
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Additional Organism Information:
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Sample Description | All unopened flower buds from primary infloroscences of 5 plants were collected 2 hours after Dex treatment. |
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Other Information:
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Timecourse start procedure | When Dex was applied to plants |
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|
Protocols for BioSource 1 |
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Slide: Yang_635-13_Dex_0h_buds_ms35 mutant_Rep2_ATH1 | | |
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Tissue:
| buds |
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Additional Organism Information:
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Sample Description | All unopened flower buds from primary infloroscences of 5 plants were collected just before Dex treatment. |
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Other Information:
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Timecourse start procedure | Just before Dex was applied to plants |
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Protocols for BioSource 1 |
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Slide: Yang_635-12_Dex _6h_buds_MYB26-ms35_Rep2_ATH1 | | |
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Tissue:
| buds |
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in vivo Treatment:
| When plants flowering with 3-5 silique (5 weeks), 25uM Dex +0.02% Silwet was spayed onto infloroscences and covered with lid. All unopened flower buds from primary infloroscences of 5 plats were collected. |
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Additional Organism Information:
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Sample Description | All unopened flower buds from primary infloroscences of 5 plants were collected at 6 hours after Dex treatment. |
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Other Information:
| |
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Timecourse start procedure | when Dex was applied to plants |
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|
Protocols for BioSource 1 |
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Problems? Comments? Suggestions? Contact the Affymetrix Team