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Experiment: Ethylene signaling enhances salt tolerance in Arabidopsis thaliana
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-659
Ethylene has been regarded as a stress hormone to regulate myriad stress responses. But how ethylene signaling is involved in the salt stress is poorly understood. To dissect the underlying molecular mechanisms, we performed transcriptome profiling of transgenic plants overexpressing EIN3 (EIN3ox), ein3eil1 double mutant and wild type Col-0. EIN3 and EIL1 are two key transcription factors in ethylene signaling pathway. For this analysis, 5-d-old seedlings treated with or without 200 mM NaCl for 6 h were used for RNA preparation. This design enabled us to examine the overlap in expression pattern among these plants, as well as to identify salt-induced and EIN3/EIL1-dependent genes.
About the ExperimenterName: | Dr. Zhonghai Li |
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Head of Lab Name: | Professor Hongwei Guo |
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Lab:
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Address: | College of Life Sciences Peking University Beijing, 100871 P.R.China
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Postcode:
| 100871 |
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Country:
| P.R.China |
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work based on any of this data, please acknowledge us, contact the
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co-authors in the work.
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| About this ExperimentExperiment Type:
| growth_condition_design; |
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Number of Slides: | 6 |
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| Experimental Parameters:
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parameter | Salt stress |
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Quality Control Measures Taken:
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Li: Ethylene signaling enhances salt tolerance in Arabidopsis thaliana
Slide: Li_659-Col-0_Control_Rep_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Li_659-Col-0_NaCl_Rep_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | 5-d-old seedlings are treated with 200 mM NaCl for 6 hours |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Li_659-ein3eil1_Control_Rep_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | 5-d-old seedlings are treated with 200 mM NaCl for 6 hours |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Li_659-ein3eil1_NaCl_Rep_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | 5-d-old seedlings are treated with 200 mM NaCl for 6 hours |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Li_659-EIN3ox_Control_Rep_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | 5-d-old seedlings are treated with 200 mM NaCl for 6 hours |
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Other Information:
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ecotype_habitat | |
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Protocols for BioSource 1 |
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Slide: Li_659-EIN3ox_NaCl_Rep_ATH1 | | |
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Protocols for BioSource 1 |
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