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Experiment: Response to gamma-irradiation in Arabidopsis thaliana mutant and wild-type seedlings
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-487
Background: At5g24280 is an ATM dependent gamma-irradiation inducible gene (Culligan et al., The Plant Journal, 2006). To evaluate its possible role in the transcriptional response upon ionizing radiation, we collected total RNA from wildtype and one At5g24280 T-DNA insertion line upon gamma-irradiation. Experimental Setup: Seeds were sterilized, washed and imbibed in ddH2O for 3 days at 4°C, sown on 1/2 MS plates and grown under continuous light at 22°C for 5 days. Plants were irradiated using a Cs137 source (100Gy), returned back into the growth cabinet and harvested 1.5h post irraditaion. RNA was extracted from irradiated and control seedlings using Tri-Reagent (Sigma), then DNase-treated with TurboDNase (Ambion) following the manufacturers suggestions. Additional purification with PCI /CI extraction was followed by EtOH-precipitation. Strains: wildtype plants and the At5g24280 T-DNA insertion line (SALK_036931) are in a Columbia (Col-O) background.
About the ExperimenterName: | Dr Gudrun Boehmdorfer |
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Head of Lab Name: | Dr Dieter Schweizer |
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Lab:
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Address: | GMI Gregor Mendel Institute of Molecular Plant Biology Dr. Bohr-Gasse 3 Vienna
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Postcode:
| 1030 |
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Country:
| Austria |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| compound_treatment_design;time_series_design |
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Number of Slides: | 18 |
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| Experimental Parameters:
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parameter | timepoint |
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parameter | irradiate |
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Quality Control Measures Taken:
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References:
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Boehmdorfer: response to gamma-irradiation in mutant and wild-type seedlings_genome
Slide: Boehmdorfer_1-16_mutant-1.5h-untreated_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | 5 day old seedlings |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Boehmdorfer_1-15_wt-24h-post-irradiation_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| 5 day old seedlings were exposed to 100Gy of gamma irradiation using a Cs137 source, then returned to the growth cabinet and harvested 24h post irradiation. |
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Additional Organism Information:
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Sample Description | 5 day old seedlings |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Boehmdorfer_1-14_wt-1.5h-post-irradiation_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| 5 day old seedlings were exposed to 100Gy of gamma irradiation using a Cs137 source, then returned to the growth cabinet and harvested 1.5h post irradiation. |
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Additional Organism Information:
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Sample Description | 5 day old seedlings |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Boehmdorfer_1-8_wt-1.5h-post-irradiation_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| 5 day old seedlings were exposed to 100Gy of gamma irradiation using a Cs137 source, then returned to the growth cabinet and harvested 1.5h post irradiation. |
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Additional Organism Information:
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Sample Description | 5 day old seedlings |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Boehmdorfer_1-9_wt-24h-post-irradiation_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| 5 day old seedlings were exposed to 100Gy of gamma irradiation using a Cs137 source, then returned to the growth cabinet and harvested 24h post irradiation. |
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Additional Organism Information:
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Sample Description | 5 day old seedlings |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Boehmdorfer_1-10_mutant-1.5h-untreated_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| 5 day old seedlings were exposed to 100Gy of gamma irradiation using a Cs137 source, then returned to the growth cabinet and harvested 1.5h post irradiation. |
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Additional Organism Information:
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Sample Description | 5 day old seedlings |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Boehmdorfer_1-11_mutant-1.5h-post-irradiation_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| 5 day old seedlings were exposed to 100Gy of gamma irradiation using a Cs137 source, then returned to the growth cabinet and harvested 1.5h post irradiation. |
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Additional Organism Information:
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Sample Description | 5 day old seedlings |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Boehmdorfer_1-12_mutant-24h-post-irradiation_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| 5 day old seedlings were exposed to 100Gy of gamma irradiation using a Cs137 source, then returned to the growth cabinet and harvested 24h post irradiation. |
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Additional Organism Information:
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Sample Description | 5 day old seedlings |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Boehmdorfer_1-13_wt-1.5h-untreated_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | 5 day old seedlings |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Boehmdorfer_1-7_wt-1.5h-untreated_Rep2_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | 5 day old seedlings |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Boehmdorfer_1-6_mutant-24h-post-irradiation_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Seedlings were gamma-irradiated using a Cs137 source (100Gy), returned to the growth cabinet and harvested 24h post irradiation. |
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Additional Organism Information:
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Sample Description | 5 day old seedling |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Boehmdorfer_1-17_mutant-1.5h-post-irradiation_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| 5 day old seedlings were exposed to 100Gy of gamma irradiation using a Cs137 source, then returned to the growth cabinet and harvested 1.5h post irradiation. |
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Additional Organism Information:
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Sample Description | 5 day old seedlings |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Boehmdorfer_1-18_mutant-24h-post-irradiation_Rep3_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Seedlings were gamma-irradiated using a Cs137 source (100Gy), returned to the growth cabinet and harvested 24h post irradiation. |
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Additional Organism Information:
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Sample Description | 5 day old seedling |
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Other Information:
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Protocols for BioSource 1 |
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Hybridisation Set: Boehmdorfer: response to gamma-irradiation in mutant and wild-type seedlings_genome
Slide: Boehmdorfer_1-1_wt-1.5h-untreated_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | 5 day old seedling |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Boehmdorfer_1-2_wt-1.5h-post-irradiation_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Seedlings were gamma-irradiated using a Cs137 source (100Gy), returned to the growth cabinet and harvested 1.5h post irradiation. |
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Additional Organism Information:
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Sample Description | 5 day old seedling |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Boehmdorfer_1-3_wt-24h-post-irradiation_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Seedlings were gamma-irradiated using a Cs137 source (100Gy), returned to the growth cabinet and harvested 24h post irradiation. |
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Additional Organism Information:
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Sample Description | 5 day old seedling |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Boehmdorfer_1-4_mutant-1.5h-untreated_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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Additional Organism Information:
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Sample Description | 5 day old seedling |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Boehmdorfer_1-5_mutant-1.5h-post-irradiation_Rep1_ATH1 | | |
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Tissue:
| whole plant |
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in vivo Treatment:
| Seedlings were gamma-irradiated using a Cs137 source (100Gy), returned to the growth cabinet and harvested 1.5h post irradiation. |
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Additional Organism Information:
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Sample Description | 5 day old seedling |
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Other Information:
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Protocols for BioSource 1 |
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