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Experiment: Priming for enhanced MeJA-responsive gene expression in Arabidopsis plants expressing P. fluorescens WCS417r-mediated ISR
Experiment Description
NASCArrays Experiment Reference Number: NASCARRAYS-463
Upon appropriate stimulation, plants can develop an enhanced capacity to express infection-induced cellular defense responses, a phenomenon known as the primed state. Colonization of the roots of Arabidopsis thaliana by the beneficial rhizobacterial strain Pseudomonas fluorescens WCS417r primes the leaf tissue for enhanced pathogen- and insect-induced expression of jasmonate (JA)-responsive genes, resulting in an induced systemic resistance (ISR) that is effective against different types of pathogens and insect herbivores. Here we investigated the molecular mechanism of this rhizobacteria-induced priming response by following a whole-genome transcript profiling approach. To this end we profiled the transcriptome of the leaves of 5-week-old Arabidopsis Col-0 plants at 0, 1, 3, and 6 hours after exogenous application of 50 µM MeJA. This was done with Col-0 plants that were grown in soil with or without ISR-inducing Pseudmonas fluorescens WCS417r rhizobacteria. This microarray analysis is described in Pozo, M.J., Van der Ent, S., Van Loon, L.C. and Pieterse, C.M.J. (2008). The transcription factor MYC2 is involved in priming for enhanced defense during rhizobacteria-induced systemic resistance in Arabidopsis. New Phytologist, in press.
About the ExperimenterName: | Prof. Corne Pieterse |
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Head of Lab Name: | Corne Pieterse |
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Lab:
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Address: | Plant-Microbe Interactions Department of Biology Utrecht University Padualaan 8 Utrecht
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Postcode:
| 3584 CH |
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Country:
| Netherlands |
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| Telephone Number:
| 0302536887 |
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Fax Number:
| 0302532837 |
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All of the data available in this website/database is free, and you
are free to do whatever you please with it. If you intend to publish
work based on any of this data, please acknowledge us, contact the
experimenter above, and either acknowledge them or use them as
co-authors in the work.
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| About this ExperimentExperiment Type:
| compound_treatment_design; time_series_design;pathogenicity_design |
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Number of Slides: | 8 |
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| Experimental Parameters:
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parameter | compound_based_treatment |
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parameter | infect |
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parameter | timepoint |
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Quality Control Measures Taken:
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References:
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reference | Pozo, M.J., Van der Ent, S., Van Loon, L.C. and Pieterse, C.M.J. (2008) |
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| Other Information:
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Slides in this Experiment
Hybridisation Set: Pieterse: Priming for enhanced MeJA-responsive gene expression in Arabidopsis plants expressing Pseudomonas fluorescens WCS417r-mediated ISR_genome
Slide: Pieterse_2-1_Control-0h_Rep1_ATH1 | | |
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Tissue:
| whole rosette |
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in vivo Treatment:
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Treatment protocol | Leaves were dipped in a solution containing 50 µM MeJA and 0.015% of Silwet L-77 |
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Separation Technique:
| Rosette was cut with a razor blade from the root system. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Pieterse_2-2_Control-1h_Rep1_ATH1 | | |
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Tissue:
| whole rosette |
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in vivo Treatment:
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Treatment protocol | Leaves were dipped in a solution containing 50 µM MeJA and 0.015% of Silwet L-77 |
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Separation Technique:
| Rosette was cut with a razor blade from the root system. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Pieterse_2-3_Control-3h_Rep1_ATH1 | | |
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Tissue:
| whole rosette |
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in vivo Treatment:
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Treatment protocol | Leaves were dipped in a solution containing 50 µM MeJA and 0.015% of Silwet L-77 |
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Separation Technique:
| Rosette was cut with a razor blade from the root system. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Pieterse_2-4_Control-6h_Rep1_ATH1 | | |
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Tissue:
| whole rosette |
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in vivo Treatment:
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Treatment protocol | Leaves were dipped in a solution containing 50 µM MeJA and 0.015% of Silwet L-77 |
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Separation Technique:
| Rosette was cut with a razor blade from the root system. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Pieterse_2-5_ISR-0h_Rep1_ATH1 | | |
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Tissue:
| whole rosette |
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in vivo Treatment:
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Treatment protocol | Plants were grown in soil containing ISR-inducing Pseudomonas fluorescens WCS417r bacteria to a final density of 5x10E7 cfu per gram of soil. When 5 weeks old, plants were dipped were dipped in a solution containing 50 µM MeJA and 0.015% of Silwet L-77. |
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Separation Technique:
| Rosette was cut with a razor blade from the root system. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Pieterse_2-6_ISR-1h_Rep1_ATH1 | | |
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Tissue:
| whole rosette |
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in vivo Treatment:
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Treatment protocol | Plants were grown in soil containing ISR-inducing Pseudomonas fluorescens WCS417r bacteria to a final density of 5x10E7 cfu per gram of soil. When 5 weeks old, plants were dipped were dipped in a solution containing 50 µM MeJA and 0.015% of Silwet L-77. |
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Separation Technique:
| Rosette was cut with a razor blade from the root system. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Pieterse_2-7_ISR-3h_Rep1_ATH1 | | |
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Tissue:
| whole rosette |
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in vivo Treatment:
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Treatment protocol | Plants were grown in soil containing ISR-inducing Pseudomonas fluorescens WCS417r bacteria to a final density of 5x10E7 cfu per gram of soil. When 5 weeks old, plants were dipped were dipped in a solution containing 50 µM MeJA and 0.015% of Silwet L-77. |
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Separation Technique:
| Rosette was cut with a razor blade from the root system. |
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Other Information:
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Protocols for BioSource 1 |
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Slide: Pieterse_2-8_ISR-6h_Rep1_ATH1 | | |
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Tissue:
| whole rosette |
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in vivo Treatment:
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Treatment protocol | Plants were grown in soil containing ISR-inducing Pseudomonas fluorescens WCS417r bacteria to a final density of 5x10E7 cfu per gram of soil. When 5 weeks old, plants were dipped were dipped in a solution containing 50 µM MeJA and 0.015% of Silwet L-77. |
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Separation Technique:
| Rosette was cut with a razor blade from the root system. |
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Other Information:
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Protocols for BioSource 1 |
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